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991.
Peter Geck Jozsef Szelei Jesus Jimenez Tien-Min Lin Carlos Sonnenschein Ana M. Soto 《The Journal of steroid biochemistry and molecular biology》1997,63(4-6):211-218
Androgens control cell numbers in the prostate through three separate pathways: (a) inhibition of cell death, (b) induction of cell proliferation (Step-1) and (c) inhibition of cell proliferation (Step-2, proliferative shutoff). The mechanisms underlying these phenomena are incompletely understood. The human prostate carcinoma LNCaP variants express these pathways as follows: LNCaP-FGC express both steps, LNCaP-LNO expresses Step-2, LNCaP-TAC expresses Step-1, and LNCaP-TJA cells express neither step. These cells facilitated the search for mediators of the androgen-induced proliferative shutoff pathway. Androgen exposure for 24 h or longer induced an irreversible proliferative shutoff in LNCaP-FGC cells. The Wang and Brown approach for identifying differentially expressed mRNAs was used to search for mediators of Step-2. Ten unique inserts were identified and from those ten, three genes were further studied. The basal expression of these genes in shutoff-negative variants was not affected by androgen exposure. They were induced by androgens in shutoff-positive LNCaP variants and the androgen receptor-transfected, shutoff-positive, MCF7-AR1 cells. These genes were induced only in the range of androgen concentrations that elicited the shutoff response. Time course analysis showed that their induction precedes the commitment point by 12–18 h. In addition, they were expressed in the normal prostate during proliferative shutoff. These features suggest that the candidate genes have a role in the regulation cascade for proliferative shutoff. 相似文献
992.
993.
Ivette Perfecto John Vandermeer Paul Hanson Victor Cartín 《Biodiversity and Conservation》1997,6(7):935-945
The coffee (Coffea arabica) agro-ecosystem in the Central Valley of Costa Rica was formerly characterized by a high vegetational diversity. This complex system has been undergoing a major transformation to capital-intensive monocultural plantations where all shade trees are eliminated. In this study we examined the pattern of arthropod biodiversity loss associated with this transformation. Canopy arthropods were sampled in three coffee farms: a traditional plantation with many species of shade trees, a moderately shaded plantation with only Erythrina poeppigeana and coffee, and a coffee monoculture. An insecticidal fogging technique was used to sample both canopy and coffee arthropods. Data are presented on three major taxonomic groups: Coleoptera, non-formicid Hymenoptera, and Formicidae. Data demonstrate that the transformation of the coffee agro-ecosystem results in a significant loss of biological diversity of both canopy arthropods as well as arthropods living in coffee bushes. Percentage of species overlap was very small for all comparisons. Furthermore, species' richness on a per tree basis was found to be within the same order of magnitude as that reported for trees in tropical forests. If results presented here are generalizable, this means that conservation efforts to preserve biological diversity should also include traditional agro-ecosystems as conservation units. 相似文献
994.
L. Jiménez M.J. de la Torre F. Maestre J.L. Ferrer I. Pérez 《Bioresource technology》1997,60(3):199-205
A central composite design was used to investigate the influence of the cooking conditions (time, temperature and phenol concentration) for wheat straw with phenol-water mixtures on the properties of the pulp obtained (yield and holocellulose, -cellulose, lignin and ethanol-benzene extractable contents) and the pH of the resulting wastewater. A second-order polynomial model consisting of three independent process variables was found to accurately describe the organosolv pulping of wheat straw. The equations derived predict the yield, the holocellulose, -cellulose, lignin and ethanol-benzene extractable contents of the pulp, and the pH of the wastewater with multiple-R, R2 and adjusted-R2 high values. The process variables must be set at low variables in order to ensure a high yield and pH. Conversely, if high holocellulose and -cellulose contents, and low lignin and ethanol-benzene extractable contents are desired, then a high temperature (200°C), long cooking time (120 min), and intermediate phenol concentration (65%) must be used. 相似文献
995.
Hydrological cycle and interannual variability of the aquatic community in a temporary saline lake (Fuente de Piedra,Southern Spain) 总被引:4,自引:2,他引:2
García Carlos M. García-Ruiz Roberto Rendón Manuel Niell F. Xavier Lucena Juan 《Hydrobiologia》1997,345(2-3):131-141
Fuente de Piedra is a shallow, temporary saline lake whoseseasonal behavior is strongly dependent on the annual hydrologicalbudget. In this study, we outline the characteristics of Fuente dePiedra Lake for two years that had different hydrological budgets.The high precipitations in 1989–90 caused the lake not to dry asusual, and decreased both salinity and the amplitude of changes.There were also differences in nutrient dynamics, with generallylower concentrations of soluble reactive phosphorus and ammonium,whereas in the more humid year nitrate showed a distinct maximum inwinter. Winter bloom chlorophyll a concentrations were alsomuch higher in 1989–90 (>600 g l-1) but there wasalso a winter productive phase that was presumably poorly coupledwith consumption processes that predominate in spring. Planktonicassemblages were different between years. Highly halotolerantphytoplankton species (Dunaliella salina and D. viridis) became scarcer, and especially two previouslyunrecorded diatoms (Cyclotella sp. and Chaetoceros sp.)became dominant in the bloom time in the wet year. The speciesrichness of the zooplankton increased in the wet year, with newspecies appearing that were not collected during 1987–88(Branchinectella media, Daphnia mediterranea, Macrothrix sp.,Arctodiaptomus salinus, Cyclops sp., Sigara sp...).There was also a much higher development of macrophytes (Ruppiadrepanensis, Althenia orientalis, Lamprothamnium papulosum)and bird populations, especially flamingoes (Phoenicopterusruber).Important interannual variations in this sort of system pointto the need for long term studies to observe the whole range ofstates that define the lake as an entity. 相似文献
996.
Spinalona anophtalma n. gen. n. sp. is describedon parthenogenetic and ephippial females andmales from material collected in a temporary lagoonlocated in the Neovolcanic Province from Mexico at analtitude of 2507 m above sea level. It ischaracterized by a strong armature of the antenna,postabdomen and postabdominal claw, no compound eyeor ocellus, the exopod of thoracic limb IIIwith only four setae and that of P5 with only three setae.This new taxon has no relationwith blind Alona from hypogean habitats. 相似文献
997.
A new genus of Platycopioida is described from a boxcore sample taken at a depth of 534 m in the ArcticBarents Sea. This is the deepest record ofPlatycopioida so far. Sarsicopia gen. n. is thesistergroup of a taxon comprising Platycopia and Nanocopia; the sistergroup ofthese is Antrisocopia. Sarsicopia gen. n.is the only platycopioid to retain 2 inner setae onthe second endopod segment P2–P4, and 8 setae in thethird endopod segment of P2. The male antennnule isremarkable in having a geniculation located betweenancestral segments XX and XXI. It is suggested thatthis flexure zone was already present in thegroundpattern of Copepoda. Platycopia and Nanocopia have secondarily lost thisgeniculation. 相似文献
998.
Several aspects of community organization wereanalyzed comparatively in a small side-arm of theParaná River (Correntoso) and a shallowfloodplain lake (El Tigre) (31° 41 S and60° 42 W), in relation to the hydrology of thesystem. Taxonomic and morphological composition inthe river differed from that in the lake: the riverhad lower species richness (151 vs 218),different contributions of some Classes to totalspecies number (higher Cyano-, Zygo- andDiatomophyceae vs higher Chlorophyceae), anddiffent proportions of nannoplanktonic algae (67.5%vs 80.7%) and netplanktonic filamentousspecies (18.2% vs 4.2%). Phytoplanktonbiomass, higher in the lake than in the river due tothe retention time, was mostly dominated bynannoplankton and netplankton. Loticphytoplankton was dominated by typical fluvialspecies of Diatomophyceae (R-strategists). Riverconditions seem to maintain a subclimacticcommunity, which was little impacted by the flushingof populations from floodplain lakes. Water levelwas the main factor controlling phytoplanktonbiomass, species diversity (H), evenness (E) andcommunity change rate () in the river. Inthe lake, phytoplankton had an autogenicsuccessional sequence during the isolation phase (C-to S-strategists) and other responses todisturbance, mainly during the flood(R-strategists). Frequent changes in phytoplanktoncomposition, biomass, H, E and , revealed aenvironmental instability in the lake, which may beexplained by interactions of external factors(hydrology and climatology) and those of internalorigin, such as nutrients and grazing. 相似文献
999.
Contribution of Mutation and RNA Recombination to the Evolution of a Plant Pathogenic RNA 总被引:10,自引:0,他引:10
Miguel A. Aranda Aurora Fraile Joaquín Dopazo José M. Malpica Fernando García-Arenal 《Journal of molecular evolution》1997,44(1):81-88
The nucleotide sequence of 17 variants of the satellite RNA of cucumber mosaic virus (CMV-satRNA) isolated from field-infected
tomato plants in the springs of 1989, 1990, and 1991 was determined. The sequence of each of the 17 satRNAs was unique and
was between 334 and 340 nucleotides in length; 57 positions were polymorphic. There was much genetic divergence, ranging from
0.006 to 0.141 nucleotide substitutions per site for pairwise comparisons, and averaging 0.074 for any pair. When the polymorphic
positions were analyzed relative to a secondary structure model proposed for CMV-satRNAs, it was found that there were significantly
different numbers of changes in base-paired and non–base-paired positions, and that mutations that did not disrupt base pairing
were preferred at the putatively paired sites. This supports the concept that the need to maintain a functional structure
may limit genetic divergence of CMV-satRNA. Phylogenetic analyses showed that the 17 CMV-satRNA variants clustered into two
subgroups, I and II, and evolutionary lines proceeding by the sequential accumulation of mutations were apparent. Three satRNA
variants were outliers for these two phylogenetic groups. They were shown to be recombinants of subgroup I and II satRNAs
by calculating phylogenies for different molecular regions and by using Sawyer's test for gene conversion. At least two recombination
events were required to produce these three recombinant satRNAs. Thus, recombinants were found to be frequent (∼17%) in natural
populations of CMV-satRNA, and recombination may make an important contribution to the generation of new variants. To our
knowledge this is the first report of data allowing the frequency of recombinant isolates in natural populations of an RNA
replicon to be estimated.
Received: 14 May 1996 / Accepted: 17 July 1996 相似文献
1000.
The Lipid Peroxidation Product, 4-Hydroxy-2-trans-Nonenal, Alters the Conformation of Cortical Synaptosomal Membrane Proteins 总被引:6,自引:1,他引:5
‡Ram Subramaniam ‡Fred Roediger Brad Jordan †‡§Mark P. Mattson §Jeffrey N. Keller Georg Waeg ‡§ D. Allan Butterfield 《Journal of neurochemistry》1997,69(3):1161-1169
Abstract: Alzheimer's disease (AD) is widely held to be a disorder associated with oxidative stress due, in part, to the membrane action of amyloid β-peptide (Aβ). Aβ-associated free radicals cause lipid peroxidation, a major product of which is 4-hydroxy-2- trans -nonenal (HNE). We determined whether HNE would alter the conformation of synaptosomal membrane proteins, which might be related to the known neurotoxicity of Aβ and HNE. Electron paramagnetic resonance spectroscopy, using a protein-specific spin label, MAL-6(2,2,6,6-tetramethyl-4-maleimidopiperidin-1-oxyl), was used to probe conformational changes in gerbil cortical synaptosomal membrane proteins, and a lipid-specific stearic acid label, 5-nitroxide stearate, was used to probe for HNE-induced alterations in the fluidity of the bilayer domain of these membranes. Synaptosomal membranes, incubated with low concentrations of HNE, exhibited changes in protein conformation and bilayer order and motion (fluidity). The changes in protein conformation were found to be concentration- and time-dependent. Significant protein conformational changes were observed at physiologically relevant concentrations of 1–10 µ M HNE, reminiscent of similar changes in synaptosomal membrane proteins from senile plaque- and Aβ-rich AD hippocampal and inferior parietal brain regions. HNE-induced modifications in the physical state of gerbil synaptosomal membrane proteins were prevented completely by using excess glutathione ethyl ester, known to protect neurons from HNE-caused neurotoxicity. Membrane fluidity was found to increase at higher concentrations of HNE (50 µ M ). The results obtained are discussed with relevance to the hypothesis of Aβ-induced free radical-mediated lipid peroxidation, leading to subsequent HNE-induced alterations in the structure and function of key membrane proteins with consequent neurotoxicity in AD brain. 相似文献