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101.
Halide binding by the purified halorhodopsin chromoprotein. I. Effects on the chromophore 总被引:4,自引:0,他引:4
The halorhodopsin chromoprotein, a retinal-protein complex with an apparent molecular mass of 20 kilo-daltons, exhibits all of the halide-dependent effects found for the chromophore of functional halorhodopsin in cell envelope vesicles. With increasing halide concentration (a) an alkali-dependent 580/410 nm chromophore equilibrium (attributed to reversible deprotonation of the retinal Schiff's base) is shifted toward the 580-nm chromophore and (b) the flash-induced photocycle proceeds increasingly via P520, rather than via P660. The halide-binding site(s) responsible for these effects must reside, therefore, in the chromoprotein. Chloride and bromide are about equivalent, but iodide is much less effective in these effects and in being transported. Several other anions, i.e. thiocyanate, nitrate, phosphate, and acetate, affect the absorption maximum of the chromophore but do not allow the production of P520 upon flash illumination and are not transported. However, these ions appear to compete with chloride in the flash experiments. These observations suggest that binding of anions to a relatively nonspecific site affects the protonation state of the Schiff's base in the chromophore. Either this site directly or a more specific site, connected to the first one by a sequential pathway, is involved with the photocycle intermediates and with chloride transport by halorhodopsin. 相似文献
102.
Narendra S. Goel Carlos F. Doggenweiler Richard L. Thompson 《Bulletin of mathematical biology》1986,48(2):167-187
A model based upon minimization of surface energy is proposed as an explanation for compaction and internalization of cells
during mammalian embryo development. The model is used to simulate and graphically display these phenomena on a computer. 相似文献
103.
S. L. Eduardo 《Systematic parasitology》1986,8(1):57-69
Summary Only Stephanopharynx compactus Fischoeder, 1901 is considered valid under the genus Stephanopharynx Fischoeder, 1901 and S. secundus Stunkard, 1929 and S. coilos Dollfus, 1963 are regarded as its synonyms. The species is redescribed and illustrated based on new material and scanning
electron photomicrographs of its tegumental surfaces and the internal surface of its pharyngeal pouch are provided.
Balanorchis anastrophus Fischoeder, 1901, the one and only species of the genus Balanorchis Fischoeder, 1901 is redescribed and illustrated. Photomicrographs of its tegumental surfaces as revealed by scanning electron
microscopy are given.
Additional information on Bilatorchis papillogenitalis Eduardo, 1980 is included.
Part of a thesis approved by the University of London for the award of the Ph.D. degree.
Part of a thesis approved by the University of London for the award of the Ph.D. degree. 相似文献
104.
Translational control of insulin biosynthesis. Evidence for regulation of elongation, initiation and signal-recognition-particle-mediated translational arrest by glucose. 总被引:14,自引:2,他引:12
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The biosynthesis of insulin in the islets of Langerhans is strongly controlled at the translational level by glucose. We have used a variety of experimental approaches in efforts to dissect the mechanisms underlying the stimulatory effect of glucose. To assess its effects on rates of peptide-chain elongation, isolated rat islets were labelled with [3H]leucine at different glucose concentrations in the presence or absence of low concentrations of cycloheximide. Under these conditions, at glucose concentrations up to 5.6 mM, endogenous insulin mRNA did not become rate-limiting for the synthesis of insulin, whereas stimulation of non-insulin protein synthesis was abolished by cycloheximide at all glucose concentrations, indicating either that insulin synthesis is selectively regulated at the level of elongation at glucose concentrations up to 5.6 mM, or that at these concentrations inactive insulin mRNA is transferred to an actively translating pool. Glucose-induced changes in the intracellular distribution of insulin mRNA in cultured islets were assessed by subcellular fractionation and blot-hybridization using insulin cDNA probes. At glucose concentrations above 3.3 mM, cytoplasmic insulin mRNA was increasingly transferred to fractions co-sedimenting with ribosomes, and relatively more of the ribosome-associated insulin mRNA became membrane-associated, consistent with effects of glucose above 3.3 mM on both the initiation of insulin mRNA and SRP (signal recognition particle)-mediated transfer of cytosolic nascent preproinsulin to the endoplasmic reticulum. When freshly isolated islets were homogenized and incubated with 125I-Tyr-tRNA, run-off incorporation of 125I into preproinsulin was increased by prior incubation of the islets at 16.7 mM-glucose. The addition of purified SRP receptor increased the run-off incorporation of [125I]iodotyrosine into preproinsulin, especially when the islets had been preincubated at 16.7 mM-glucose. These findings taken together suggest that glucose may stimulate elongation rates of nascent preproinsulin at concentrations up to 5.6 mM, stimulates initiation of protein synthesis involving both insulin and non-insulin mRNA at concentrations above 3.3 mM, and increases the transfer of initiated insulin mRNA molecules from the cytoplasm to microsomal membranes by an SRP-mediated mechanism that involves the modification of interactions between SRP and its receptor. 相似文献
105.
Silvia E. Fontenla de Petrino M. Eugenia B. B. de Jorrat Angel Sirena Juan Carlos Valdez Oscar Mesón 《Mycopathologia》1986,94(2):85-89
Candida albicans produces germ-tubes (GT) when it is incubated in animal or human serum. This dimorphism is responsible for its invasive ability.The purpose of the present paper is (1) to evaluate the ability of rat peritoneal macrophages to inhibit GT production of ingested Candida albicans, obtained from immunized rats and then activated in vitro with Candida-induced lymphokines; (2) to determinate any possible alteration of phagocytic and candidacidal activities.The phagocytes were obtained from rats immunized with viable C. albicans. Some of them were exposed to Candida-induced lymphokines in order to activate the macrophages in vitro. The monolayers of activated, immune and normal macrophages were infected with a C. albicans suspension during 4 hr.Activated macrophages presented not only the highest phagocytic and candidacidal activities but a noticeable inhibition of GT formation and incremented candidacidal activity. 相似文献
106.
Iron-Chelating Compounds Produced by Soil Pseudomonads: Correlation with Fungal Growth Inhibition 总被引:8,自引:3,他引:5
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Peter A. Vandenbergh Carlos F. Gonzalez Ann M. Wright Blair S. Kunka 《Applied microbiology》1983,46(1):128-132
Strains of Pseudomonas putida, Pseudomonas sp., and Pseudomonas aeruginosa were examined for their ability to grow in the presence of the iron chelator, ethylenediamine-di-(o-hydroxyphenylacetic acid). In vitro fungal inhibition assays showed that the isolates varied in their ability to inhibit the growth of representative fungal plant pathogens. Fungal inhibition in vitro was superior to that of previously reported Pseudomonas sp. Studies with Fusarium oxysporum forma sp. lycopersici and a susceptible tomato cultivar demonstrated that Pseudomonas putida PPU3.1 was able to significantly reduce wilt disease. 相似文献
107.
Factors Determining Annual Changes in Bacterial Photosynthetic Pigments in Holomictic Lake Cisó, Spain
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The pigments and biomass of anoxygenic phototrophic bacteria were measured during a year cycle in Lake Cisó (Girona, Spain). Two genera, Chromatium and Chlorobium, accounted for most of the bacterial population. The bacteria were present throughout the year despite complete mixing of the lake during fall and winter. This was possible because the sulfide production in the sediment was high enough to make the lake anaerobic to the very surface. Solar radiation, temperature, and biomass of Chromatium sp. were found to be important in determining pigment concentrations by correlation analysis. Sulfide concentration and biomass of Chlorobium spp. were found to be unimportant. A path analysis was performed to determine what percentage of the variability of pigments could be explained by the variables studied. Since a high percentage could be explained, it was possible to conclude that solar radiation, temperature, and biomass of Chromatium sp. were the main variables. 相似文献
108.
Jorge H. Medina María Laura Novas† Eduardo De Robertis† 《Journal of neurochemistry》1983,41(3):703-709
Abstract: This study was designed to analyze possible differences in the binding of [3H]flunitrazepam ([3H]FNZP) and [3H]ethyl - β - carboline - 3 - carboxylate ([3H]β-CCE), to rat brain membranes, in various experimental conditions. In cerebral cortex, hippocampus, cerebellum, and orain stem the number of binding sites for [3H]β-CCE was higher than for [3H]FNZP; both were displaced by clonazepam. Until the 7th day of postnatal brain development the numbers of [3H]FNZP and [3H]β-CCE sites were equivalent; but later on, the β-carboline sites increased to a higher level. Noradrenergic denervation by 6-hydroxydopamine was followed in the hippocampal formation. Already after 2 days, there was a decrease in [3H]FNZP sites, which reached 70% of control after 14 days. Similar results were obtained with DSP-4 denervation. This change was only in Bmax and not in KD, In contrast, the [3H]β-CCE sites did not change with denervation. Neonatal injection of l - 2,4,5 - trihydroxyphenylalamine or DSP-4 produced in the adult a decrease in [3H]FNZP sites in the cerebral cortex, in parallel with the noradrenergic denervation. On the other hand, there was an increase in the cerebellum and brain stem, in correspondence with the hyperinnervation by sprouting. In these rats, the number of sites for [3H]β-CCE did not change in the different brain regions. With 0.1% Triton X-100, applied to synaptosomal membranes, [3H]FNZP binding was reduced by 35%, while that of [3H]β-CCE was not significantly changed. These results suggest that there is heterogeneity of binding sites for benzodiazepine receptors in rat brain. A tentative interpretation of the experiments involving noradrenergic denervation and hyperinnervation, as well as those with Triton X-100, is that [3H]FNZP binds to pre- and postsynaptic receptors, while [3H]β-CCE binds mainly to postsynaptic benzodiazepine receptors. 相似文献
109.
Carlos A. Braumann 《Bulletin of mathematical biology》1983,45(4):635-641
This paper reviews some recent advances in single population stochastic differential equation growth models. They are a natural way to model population growth in a randomly varying environment. The question of which calculus, Itô or Stratonovich, is preferable is addressed. The two calculi coincide when the noise term is linear, if we take into account the differences in the interpretation of the parameters. This clarifies, among other things, the controversy on the theory of niche limiting similarity proposed by May and MacArthur. The effects of correlations in the environmental fluctuations and statistical methods for estimating parameters and for prediction based on a single population trajectory are mentioned. Applications to fisheries, wildlife management and particularly to environmental impact assessment are now becoming possible and are proposed in this paper. 相似文献
110.
S. L. Eduardo 《Systematic parasitology》1983,5(1):25-79
Summary The genus Calicophoron N?smark, 1937 is redefined. Only the following species originally assigned by N?smark to the genus are considered valid:
C. calicophorum (Fischoeder, 1901) N?smark, 1937 (type species); C. papillosum (Stiles & Goldberger, 1910) N?smark, 1937; and C. raja N?smark, 1937.
The following species previously assigned to the genus Paramphistomum Fischoeder, 1901 are considered valid but moved to the genus Calicophoron N?smark, 1937 in new combinations: C. bothriophoron (Braun, 1892); C. microbothrium (Fischoeder, 1901); C. papilligerum (Stiles & Goldberger, 1910); C. clavula (N?smark, 1937); C. microbothrioides (Price & McIntosh, 1944); C. sukari (Dinnik, 1954); C. phillerouxi (Dinnik, 1961); C. daubneyi (Dinnik, 1962) and C. sukumum (Dinnik, 1964).
The genus Bothriophoron (Stiles & Goldberger, 1910) Grétillat 1958 is synoymized with Calicophoron N?smark, 1937. Paramphistomum crassum Stiles & Goldberger, 1910, P. cauliorchis Stiles & Goldberger, 1910, P. ijimai Fukui, 1922, P. (Cauliorchis) skrjabini Popova, 1937, P. erschovi Davydova, 1959, Calicophoron orientalis Mukherjee, 1966, C. wuchengense Wang, 1979 and Cotylophoron skrjabini Mitskevich, 1958 in part are considered synonyms of Calicophoron calicophorum (Fischoeder, 1901) N?smark, 1937. C. zhejiangense Wang, 1979 is regarded a synonym of C. papillosum (Stiles & Goldberger, 1910) N?smark, 1937. P. vangrembergeni Van Strydonck, 1970 and P. togolense Albaret, Bayssade-Dufour, Guilhon, Kulo & Picot, 1978 are considered synonyms of Calicophoron phillerouxi (Dinnik, 1961) n. comb. and Ceylonocotyle petrovi Davydova, 1961 is synonymized with Calicophoron microbothrioides (Price & McIntosh, 1944) n. comb.
The species considered valid under the genus are redescribed and illustrated and scanning electron photomicrographs of the
tegumental surfaces of some species are provided. A key to the species is given.
Part of a thesis approved by the University of London for the award of the Ph.D. degree. 相似文献