全文获取类型
收费全文 | 608篇 |
免费 | 52篇 |
专业分类
660篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 13篇 |
2020年 | 4篇 |
2019年 | 11篇 |
2018年 | 16篇 |
2017年 | 11篇 |
2016年 | 22篇 |
2015年 | 30篇 |
2014年 | 31篇 |
2013年 | 44篇 |
2012年 | 48篇 |
2011年 | 62篇 |
2010年 | 35篇 |
2009年 | 33篇 |
2008年 | 40篇 |
2007年 | 40篇 |
2006年 | 27篇 |
2005年 | 28篇 |
2004年 | 23篇 |
2003年 | 21篇 |
2002年 | 29篇 |
2001年 | 7篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1998年 | 11篇 |
1997年 | 9篇 |
1996年 | 5篇 |
1994年 | 6篇 |
1993年 | 5篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 7篇 |
1984年 | 1篇 |
1978年 | 1篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有660条查询结果,搜索用时 15 毫秒
181.
182.
MoDEL (Molecular Dynamics Extended Library): a database of atomistic molecular dynamics trajectories
Meyer T D'Abramo M Hospital A Rueda M Ferrer-Costa C Pérez A Carrillo O Camps J Fenollosa C Repchevsky D Gelpí JL Orozco M 《Structure (London, England : 1993)》2010,18(11):1399-1409
More than 1700 trajectories of proteins representative of monomeric soluble structures in the protein data bank (PDB) have been obtained by means of state-of-the-art atomistic molecular dynamics simulations in near-physiological conditions. The trajectories and analyses are stored in a large data warehouse, which can be queried for dynamic information on proteins, including interactions. Here, we describe the project and the structure and contents of our database, and provide examples of how it can be used to describe the global flexibility properties of proteins. Basic analyses and trajectories stripped of solvent molecules at a reduced resolution level are available from our web server. 相似文献
183.
184.
F. Lézot B.L. Thomas C. Blin‐Wakkach B. Castaneda A. Bolanos D. Hotton P.T. Sharpe D. Heymann G.F. Carles A.E. Grigoriadis A. Berdal 《Journal of cellular physiology》2010,223(3):779-787
Skeletal growth and homeostasis require the finely orchestrated secretion of mineralized tissue matrices by highly specialized cells, balanced with their degradation by osteoclasts. Time‐ and site‐specific expression of Dlx and Msx homeobox genes in the cells secreting these matrices have been identified as important elements in the regulation of skeletal morphology. Such specific expression patterns have also been reported in osteoclasts for Msx genes. The aim of the present study was to establish the expression patterns of Dlx genes in osteoclasts and identify their function in regulating skeletal morphology. The expression patterns of all Dlx genes were examined during the whole osteoclastogenesis using different in vitro models. The results revealed that Dlx1 and Dlx2 are the only Dlx family members with a possible function in osteoclastogenesis as well as in mature osteoclasts. Dlx5 and Dlx6 were detected in the cultures but appear to be markers of monocytes and their derivatives. In vivo, Dlx2 expression in osteoclasts was examined using a Dlx2/LacZ transgenic mouse. Dlx2 is expressed in a subpopulation of osteoclasts in association with tooth, brain, nerve, and bone marrow volumetric growths. Altogether the present data suggest a role for Dlx2 in regulation of skeletal morphogenesis via functions within osteoclasts. J. Cell. Physiol. 223:779–787, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
185.
In temporary streams, the annual constriction of drying is associated with high local extinction risk. To survive in such
habitats, organisms with no specific biological traits for coping with dry periods should experience high colonisation rates
from permanent reaches of the same basin or from other basins. Hydropsyche siltalai is a widespread caddisfly common in permanent and temporary headwaters reaches in the Mediterranean climate region of the
Iberian Peninsula. In this study, we used genetic analyses to test if populations of H. siltalai in temporary streams are resettled from populations of the same basin or from other basins. The geographical distribution
of H. siltalai was surveyed in 97 temporary and permanent reaches across four basins; larvae were found in 22 reaches (12 temporary and
10 permanent). Population genetic analyses of 11 selected reaches (6 temporary and 5 permanent) revealed low genetic diversity
and no genetic population structure among and within basins. Overall, H. siltalai appeared to disperse well among basins independent of stream temporality. Permanent reaches from different basins act as
a source of the individuals that recolonise temporary reaches after local extinctions, indicating a metapopulation structure
at regional scale. Moreover, our results support other studies that showed that dispersal among basins is a recurrent pattern
in aquatic insects. 相似文献
186.
Jérémie Roux Michel Carles Hidefumi Koh Arnaud Goolaerts Michael T. Ganter Brian B. Chesebro Marybeth Howard Benjamin T. Houseman Walter Finkbeiner Kevan M. Shokat Agnès C. Paquet Michael A. Matthay Jean-Fran?ois Pittet 《The Journal of biological chemistry》2010,285(7):4278-4290
Exogenous or endogenous β2-adrenergic receptor agonists enhance alveolar epithelial fluid transport via a cAMP-dependent mechanism that protects the lungs from alveolar flooding in acute lung injury. However, impaired alveolar fluid clearance is present in most of the patients with acute lung injury and is associated with increased mortality, although the mechanisms responsible for this inhibition of the alveolar epithelial fluid transport are not completely understood. Here, we found that transforming growth factor β1 (TGF-β1), a critical mediator of acute lung injury, inhibits β2-adrenergic receptor agonist-stimulated vectorial fluid and Cl− transport across primary rat and human alveolar epithelial type II cell monolayers. This inhibition is due to a reduction in the cystic fibrosis transmembrane conductance regulator activity and biosynthesis mediated by a phosphatidylinositol 3-kinase (PI3K)-dependent heterologous desensitization and down-regulation of the β2-adrenergic receptors. Consistent with these in vitro results, inhibition of the PI3K pathway or pretreatment with soluble chimeric TGF-β type II receptor restored β2-adrenergic receptor agonist-stimulated alveolar epithelial fluid transport in an in vivo model of acute lung injury induced by hemorrhagic shock in rats. The results demonstrate a novel role for TGF-β1 in impairing the β- adrenergic agonist-stimulated alveolar fluid clearance in acute lung injury, an effect that could be corrected by using PI3K inhibitors that are safe to use in humans. 相似文献
187.
188.
189.
Alvaro Cervera Anna M. Planas Carles Justicia Xabier Urra Jens C. Jensenius Ferran Torres Francisco Lozano Angel Chamorro 《PloS one》2010,5(2)
Background
The complement system is a major effector of innate immunity that has been involved in stroke brain damage. Complement activation occurs through the classical, alternative and lectin pathways. The latter is initiated by mannose-binding lectin (MBL) and MBL-associated serine proteases (MASPs). Here we investigated whether the lectin pathway contributes to stroke outcome in mice and humans.Methodology/Principal Findings
Focal cerebral ischemia/reperfusion in MBL-null mice induced smaller infarctions, better functional outcome, and diminished C3 deposition and neutrophil infiltration than in wild-type mice. Accordingly, reconstitution of MBL-null mice with recombinant human MBL (rhMBL) enhanced brain damage. In order to investigate the clinical relevance of these experimental observations, a study of MBL2 and MASP-2 gene polymorphism rendering the lectin pathway dysfunctional was performed in 135 stroke patients. In logistic regression adjusted for age, gender and initial stroke severity, unfavourable outcome at 3 months was associated with MBL-sufficient genotype (OR 10.85, p = 0.008) and circulating MBL levels (OR 1.29, p = 0.04). Individuals carrying MBL-low genotypes (17.8%) had lower C3, C4, and CRP levels, and the proinflammatory cytokine profile was attenuated versus MBL-sufficient genotypes.Conclusions/Significance
In conclusion, genetically defined MBL-deficiency is associated with a better outcome after acute stroke in mice and humans. 相似文献190.