全文获取类型
收费全文 | 5104篇 |
免费 | 374篇 |
国内免费 | 2篇 |
出版年
2023年 | 22篇 |
2022年 | 64篇 |
2021年 | 120篇 |
2020年 | 90篇 |
2019年 | 98篇 |
2018年 | 133篇 |
2017年 | 130篇 |
2016年 | 177篇 |
2015年 | 247篇 |
2014年 | 267篇 |
2013年 | 336篇 |
2012年 | 412篇 |
2011年 | 378篇 |
2010年 | 230篇 |
2009年 | 189篇 |
2008年 | 284篇 |
2007年 | 269篇 |
2006年 | 245篇 |
2005年 | 233篇 |
2004年 | 206篇 |
2003年 | 208篇 |
2002年 | 188篇 |
2001年 | 74篇 |
2000年 | 56篇 |
1999年 | 56篇 |
1998年 | 50篇 |
1997年 | 36篇 |
1996年 | 38篇 |
1995年 | 28篇 |
1994年 | 35篇 |
1993年 | 31篇 |
1992年 | 35篇 |
1991年 | 48篇 |
1990年 | 37篇 |
1989年 | 29篇 |
1988年 | 28篇 |
1987年 | 20篇 |
1986年 | 32篇 |
1985年 | 31篇 |
1984年 | 22篇 |
1983年 | 21篇 |
1982年 | 17篇 |
1981年 | 14篇 |
1980年 | 18篇 |
1979年 | 13篇 |
1977年 | 18篇 |
1974年 | 15篇 |
1972年 | 13篇 |
1970年 | 14篇 |
1969年 | 16篇 |
排序方式: 共有5480条查询结果,搜索用时 13 毫秒
21.
A firm, mobile, oblong mass was discovered as an incidental finding in the stomach of a chimpanzee (Pan troglodytes) during routine surgery. It was removed and determined to be a trichobezoar. 相似文献
22.
The complete primary structure of donkey lysozyme has been established by pulsed liquid-phase sequencing of tryptic and chymotryptic peptides isolated by RP-HPLC. The positions of the Cys residues were identified by labeling the Cys residues with DABIA-reagent. Donkey lysozyme is a c-type lysozyme which is 129 amino acids long. It exhibits 50% homology to the human protein. We observe the full Ca(II) binding site suggested for the homologous alpha-lactalbumines. Although horse lysozyme has been reported to contain asparagine in position 61, which was in conflict with the three-dimensional structure of lysozyme, all other known c-type lysozymes, including donkey, contain Ser 61. 相似文献
23.
Racemic 4',6-dichloroflavan (BW683C), a highly effective inhibitor of rhinovirus serotype 1B in vitro, was resolved by high-performance liquid chromatography on a chiral stationary phase. The enantiomers were separately collected and circular dichroism curves were obtained, in order to determine the absolute configuration of the two enantiomers. The activity of the isomers was studied on human rhinovirus serotype 1B multiplication in HeLa cell cultures, by means of the plaque reduction assay. Both enantiomers were potent inhibitors of virus replication; by comparing the IC50 values, the S form was 3.5 times more effective than the R form. 相似文献
24.
Valeria Cavaliere Franco Graziani Silvia Andone Andrea Manzi Carla Malva 《Molecular & general genetics : MGG》1991,230(3):433-441
Summary The abnormal oocyte phenotype is characterized by instability, as shown by the loss and reappearance of the abo maternal effect under specific genetic conditions. Our previous finding that a correlation exists between the abo phenotype and the presence of a blood transposon in region 32E, led us to perform an extensive genetic and molecular analysis of the most significant aspects of the abo phenotype in different genetic backgrounds. The results of these experiments can be summarized as follows: Complete reversion occurs only when the blood transposon is lost, thus definitively demonstrating that the insertion of the blood transposon in region 32E is the molecular event that causes the pleiotropic abo phenotype. Partial reversion can also occur without loss of the transposon, indicating that different molecular pathways may be involved in the loss of the abo phenotype. Reappearance of the full abo phenotype can occur only in heterozygous lines constructed from partially revertant abo homozygous lines that have not lost the blood transposon. 相似文献
25.
Eugene M. Rinchik Terry Magnuson Bernadette Holdener-Kenny Gavin Kelsey Albert Bianchi Claudio J. Conti Fran?ois Chartier Kathryn A. Brown Stephen D. M. Brown Josephine Peters 《Mammalian genome》1992,3(Z1):S104-S120
Chair of Committee for Mouse Chromosome 7 相似文献
26.
1. Two mutants of the sodium channel II have been expressed inXenopus oocytes and have been investigated using the patch-clamp technique. In mutant E387Q the glutamic acid at position 387 has been replaced by glutamine, and in mutant D384N the aspartic acid at position 384 has been replaced by asparagine.2. Mutant E387Q, previously shown to be resistant to block by tetrodotoxin (Noda et al. 1989), has a single-channel conductance of 4 pS, that can be easily measured only using noise analysis. At variance with the wild-type, the openchannel current-voltage relationship of mutant E387Q is linear over a wide voltage range even under asymmetrical ionic conditions.3. Mutant D384N has a very low permeability for any of the following ions: Cl–, Na+, K+, Li+, Rb+, Ca2+, Mg2+, NH4
+ , TMA+, TEA+. However, asymmetric charge movements similar to the gating currents of the Na+-selective wild-type are still observed.4. These results suggest that residues E387 and D384 interact directly with the pathway of the ions permeating the open channel.Abbreviations TTX
tetrodotoxin; Na+, sodium; K+, potassium;
- NFR
normal frog Ringer
- HEPES
N-2-hydroxylethyl piperazine-N-2-ethanesulfonic acid
- EGTA
ethyleneglycol-bis(-amino-ethyl ether) N,N,N',N'-tetra acetic acid
- TEA
tetraethylammonium
- TMA
tetramethylammonium;I
g
, gating current; , single-channel conductance 相似文献
27.
John J. Reiners Jr. Tim Rupp Claudio J. Conti 《Differentiation; research in biological diversity》1991,47(2):69-75
In situ hybridization and immunocytochemical techniques have been used to examine the distribution of vitamin-D-induced calbindin mRNA and calbindin protein in enterocytes lining the crypts and villi of chicken small intestine. Basal villus enterocytes contained approximately twice as much calbindin but over three times as much calbindin mRNA compared to values found in basal crypt and upper villus enterocytes, all values being measured 2 days after vitamin D injection into D-deficient chickens. Virtually no calbindin mRNA was detected in tissues taken from control D-deficient birds. Direct proportionality found between calbindin mRNA and calbindin content in enterocytes of basal crypt, mid and upper villus suggests pre-translational control over calbindin synthesis. The implications of possible inefficient translation of calbindin mRNA in basal villus enterocytes are discussed. Present methods of analysis provide a novel way to study mechanisms controlling gene expression throughout the whole process of enterocyte differentiation. 相似文献
28.
29.
S K Erickson G Bruscalupi L Conti Devirgiliis S Leoni M T Mangiantini S Spagnuolo A Trentalance 《Biochimica et biophysica acta》1988,963(3):525-533
Maintenance of whole body cholesterol homeostasis is determined in part by the liver. Thus, changes in expression of hepatic parameters important in the regulation of cholesterol metabolism may play key roles in determining how homeostasis is maintained. The expression of hepatic lipoprotein uptake systems was studied during development using as a ligand very-low density lipoproteins rich in apolipoprotein E that had been obtained from hypercholesterolemic adult rats. These lipoproteins can serve as ligands for cell surface receptors recognizing apolipoproteins B and/or E. Uptake was lowest in freshly isolated fetal rat hepatocytes, increased substantially in hepatocytes from neonates and was intermediate in those from adults. Binding of these lipoproteins to liver membranes prepared from fetal, neonatal, suckling, weaned and adult rats was lowest in fetal preparations, while those from suckling, weaned and adult livers behaved similarly. Numbers of binding sites in neonatal liver membranes were similar to those in adult, but showed a different affinity. On the basis of this data, the ability of hepatocytes to recognize and remove apolipoprotein B/E-containing lipoproteins from the plasma appears to be a function of the differential expression or regulation of lipoprotein-uptake systems during development. 相似文献
30.
10 "false negative" chemical carcinogens, i.e. ineffective in bacterial mutagenicity assays, were thoroughly investigated for their genotoxic activity in the mould Aspergillus nidulans. Forward mutations (methionine suppressors), mitotic crossing-over and chromosome malsegregation were the end-points scored. Positive results were obtained in tests for the induction of mitotic segregation with benzene, ethylenethiourea and urethane, which increased the frequency of abnormal presumptive aneuploid colonies with euploid sectors showing whole chromosome segregation (i.e. non-disjunctional diploids and haploids). The same compounds were ineffective in increasing the frequency of mitotic crossing-over or forward mutations. The other chemical carcinogens investigated, namely acetamide, amitrole, dieldrin, heptachlor epoxide, nitrilotriacetic acid, p,p'-DDT and thiourea were ineffective both as inducers of forward mutations and mitotic segregation. 相似文献