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121.
A. J. Monforte M. J. Asíns E. A. Carbonell 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(4):706-713
A study of genotype-by-salinity interaction was carried out to compare the behavior of quantitative trait loci (QTLs) in
two F2 populations derived from crosses between the cherry tomato, Lycopersicon esculentum Mill. var. cerasiforme, and two wild relatives Lycopersicon pimpinellifolium (Jusl.) Mill. and Lycopersicon chesmannii f. minor (Hook. f.) Mull., grown at two environmental conditions (optimum and high salinity). QTLs for earliness and fruit yield could
be classified into four groups: “response-sensitive”, those detected only under control conditions or whose contribution significantly
decreased in salinity; “response-tolerant”, detected only in salinity or in which the direction of their additive effects
changed; “constitutive”, detected in both growing conditions; and “altered” QTLs, those where the degree of dominance changed
according to the presence or absence of salt. Epistatic interactions were also influenced by the salt treatment. This differential
allele effect at some (non-constitutive) QTLs induced by salt stress will make selection under an “optimum environment” unfruitful
for the “response-tolerant” QTLs. Similarly, selection under salinity will ignore “response-sensitive” QTLs. Given that salinity
is highly variable in the field, marker-assisted selection should take into account not only the “response-tolerant” but also
the “response-sensitive” QTLs although there might be cases where selection in some QTLs for both conditions is not feasible.
Comparing both populations, very few QTLs showed the same behavior.
Received: 5 August 1996 / Accepted: 25 October 1996 相似文献
122.
123.
Polyamines are small positively charged molecules with a widespread presence in all living organisms. In plants they modulate several aspects of growth and differentiation, and they also participate in the response to abiotic stress. Here we review the molecular mechanisms involved in the regulation of polyamine biosynthesis, which is exerted at different levels including gene expression, protein synthesis, and formation of multienzyme complexes. The importance of polyamines both in development and in stress resistance is also subtended by the phenotype of loss-of-function mutants and of overexpressing lines affecting the different genes that encode polyamine metabolism enzymes. 相似文献
124.
A. J. Monforte M. J. Asíns E. A. Carbonell 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):284-293
Salt tolerance was studied comparatively in three families derived from crosses between Lycopersicon esculentum Mill. and two related wild species [two accessions of Lycopersicon pimpinellifolium (Jusl.) Mill. and one accession of Lycopersicon chesmannii f.minor (Hook.f.) Mull.] by means of QTL analysis of fruit yield and earliness under conditions of salinity. From six polymorphic
genomic regions involved in salt tolerance, three contained segregant salt-tolerant QTLs for the three families; two were
found only in both families derived from L.pimpinellifolium; and one, involved in fruit number, was detected only in one of the L.pimpinellifolium families. Some differences regarding the effects of the wild alleles at orthologous QTLs were found. These effects were always
negative in the L. chesmannii family. Comparing both L. pimpinellifolium families, the “wild” alleles at two out of nine common QTLs for fruit number and weight had effects with opposite directions,
and the mode of gene action was clearly different at five of them. QTL analysis of earliness revealed the largest genotypic
differences among families. Most drastic differences were found for the epistatic interactions in which all genomic regions
containing QTLs were involved. These interactions between unlinked genes increased the range of variation of means, mainly
upwards, as compared with genotypes at individual QTLs. Only one (affecting fruit weight) out of 27 interactions was detected
in both L.pimpinellifolium families. Heterotic effects found for salt tolerance in one of the families can be explained by the presence of overdominant
(or pseudo-overdominant) and dominant gene effects at QTLs controlling final fruit yield under conditions of salinity. Allelic
variation at salt-tolerant QTLs exists, changing the additive and, mainly, the non-additive components of the genotypic value.
Consequently, it may negatively affect the general applicability (or efficiency) of marker-assisted selection to improve salt
tolerance in other segregant populations where QTLs were not studied. The use of more informative co-dominant markers, like
microsatelites, might overcome these problems.
Received: 5 August 1996/Accepted: 25 October 1996 相似文献
125.
Simi S Carbonell GV Falcón RM Gatti MS Joazeiro PP Darini AL Yano T 《Canadian journal of microbiology》2003,49(7):479-482
Seven of 50 Enterobacter cloacae strains from clinical isolates produced small turbid zones of hemolysis in horse and sheep blood agar plates, and the culture supernatants were also positive for hemolytic activity. The hemolysin was partially purified from the culture supernatant of E. cloacae by ultrafiltration (PM-10 membrane) and extraction with acetone. Semipurified hemolysin was stable to heating (100 degrees C, 30 min) and was soluble in organic solvents (acetone, ethanol, and methanol). The toxin showed no loss of biological activity after treatment with trypsin and was stable to acid treatment at pH 2.0 but not at a pH greater than 7.0. In the rat intestinal loop assay, the hemolysin caused hemorrhagic fluid accumulation and severe histological alterations. These findings indicate that this hemolysin may be a putative virulence factor in E. cloacae infections. 相似文献
126.
127.
Kinetic and Morphological Observations on the Yeast Phase of Histoplasma capsulatum During Protoplast Formation 总被引:2,自引:0,他引:2 下载免费PDF全文
Protoplast formation by Histoplasma capsulatum yeasts using high concentrations of MgSO(4) occurs either by lysis of the bud or lysis of the entire cell wall. Both mechanisms may also occur simultaneously. Neither the protoplast emerging through a hole in the cell wall nor the freshly released protoplast has a recognizable cell wall or the remnant of such. The protoplast contains all the organelles of the normal cell except for mesosomes. During protoplast formation the nucleus increases in size and produces several nuclear masses by the invaginations of the internal layer of the nuclear membrane. All these nuclear masses are surrounded by the external layer of the nuclear membrane. Several nuclei with a normal nuclear membrane are formed later. 相似文献
128.
Plate-cultured bacterial colonies are intriguing models to study host-parasite interactions in senescent populations. During the growth of bacteriophage-infected colonies there is a synchronous prophage induction episode among lysogenic cells that allows a dramatic but time-restricted amplification of viral particles. We report here that the dynamics of phage spread depends on the history of the lysogenic cell that establishes the clonal population, the duration of the pre-burst period being shorter when the founder, infected cell derives from older colonies. These results offer a physiologic explanation for the self-contained progression of the viral spread in closed environments, that ensures both viral dissemination but also survival of most of the host cells. 相似文献
129.
Cell proliferation in the developing rat pineal gland. A bromodeoxyuridine immunohistochemical study
The immunohistochemical detection of bromodeoxyuridine (BrdU) was used to study the cell proliferation in the developing rat pineal gland, from the appearance of pineal primordium in the embryonic day 15 (E15) until 30 days after birth. The results showed three different proliferative phases. From E15 to E21, the pineal gland shows a phase of rapid proliferation. The second phase corresponds to the first postnatal week, in which the number of labeled cells per surface unit decreases suddenly to values between 20% to 10% of those of embryonic period. From the second postnatal week onwards, the number of BrdU-positive cells progressively decreases. 相似文献
130.
Comparison of probabilistic combination methods for protein secondary structure prediction 总被引:2,自引:0,他引:2
Liu Y Carbonell J Klein-Seetharaman J Gopalakrishnan V 《Bioinformatics (Oxford, England)》2004,20(17):3099-3107
MOTIVATION: Protein secondary structure prediction is an important step towards understanding how proteins fold in three dimensions. Recent analysis by information theory indicates that the correlation between neighboring secondary structures are much stronger than that of neighboring amino acids. In this article, we focus on the combination problem for sequences, i.e. combining the scores or assignments from single or multiple prediction systems under the constraint of a whole sequence, as a target for improvement in protein secondary structure prediction. RESULTS: We apply several graphical chain models to solve the combination problem and show that they are consistently more effective than the traditional window-based methods. In particular, conditional random fields (CRFs) moderately improve the predictions for helices and, more importantly, for beta sheets, which are the major bottleneck for protein secondary structure prediction. 相似文献