全文获取类型
收费全文 | 3792篇 |
免费 | 352篇 |
国内免费 | 4篇 |
出版年
2023年 | 18篇 |
2022年 | 29篇 |
2021年 | 56篇 |
2020年 | 55篇 |
2019年 | 43篇 |
2018年 | 63篇 |
2017年 | 65篇 |
2016年 | 100篇 |
2015年 | 160篇 |
2014年 | 163篇 |
2013年 | 203篇 |
2012年 | 263篇 |
2011年 | 266篇 |
2010年 | 176篇 |
2009年 | 168篇 |
2008年 | 242篇 |
2007年 | 255篇 |
2006年 | 211篇 |
2005年 | 217篇 |
2004年 | 208篇 |
2003年 | 182篇 |
2002年 | 180篇 |
2001年 | 35篇 |
2000年 | 53篇 |
1999年 | 47篇 |
1998年 | 43篇 |
1997年 | 42篇 |
1996年 | 42篇 |
1995年 | 24篇 |
1994年 | 33篇 |
1993年 | 30篇 |
1992年 | 20篇 |
1991年 | 24篇 |
1990年 | 29篇 |
1989年 | 21篇 |
1987年 | 22篇 |
1986年 | 12篇 |
1985年 | 19篇 |
1983年 | 13篇 |
1982年 | 23篇 |
1981年 | 26篇 |
1980年 | 24篇 |
1979年 | 21篇 |
1978年 | 13篇 |
1977年 | 13篇 |
1975年 | 18篇 |
1972年 | 12篇 |
1968年 | 12篇 |
1967年 | 12篇 |
1964年 | 12篇 |
排序方式: 共有4148条查询结果,搜索用时 234 毫秒
101.
Different Glycosylation in Acetylcholinesterases from Mammalian Brain and Erythrocytes 总被引:1,自引:0,他引:1
Acetylcholinesterases (EC 3.1.1.7, AChE) have varying amounts of carbohydrates attached to the core protein. Sequence analysis of the known primary structures gives evidence for several asparagine-linked carbohydrates. From the differences in molecular mass determined on sodium dodecyl sulfate-polyacrylamide gel before and after deglycosylation with N-glycosidase F (EC 3.2.2.18), it is seen that dimeric AChE from red cell membranes is more heavily glycosylated than the tetrameric brain enzyme. Furthermore, dimeric and tetrameric forms of bovine AChE are more heavily glycosylated than the corresponding human enzymes. Monoclonal antibodies 2E6, 1H11, and 2G8 raised against detergent-soluble AChE from electric organs of Torpedo nacline timilei as well as Elec-39 raised against AChE from Electrophorus electricus cross-reacted with AChE from bovine and human brain but not with AChE from erythrocytes. Treatment of the enzyme with N-glycosidase F abolished binding of monoclonal antibodies, suggesting that the epitope, or part of it, consists of N-linked carbohydrates. Analysis of N-acetylglucosamine sugars revealed the presence of N-acetylglucosamine in all forms of cholinesterases investigated, giving evidence for N-linked glycosylation. On the other hand, N-acetylgalactosamine was not found in AChE from human and bovine brain or in butyrylcholinesterase (EC 3.1.1.8) from human serum, indicating that these forms of cholinesterase did not contain O-linked carbohydrates. Despite the notion that within one species, the different forms of AChE arise from one gene by different splicing, our present results show that dimeric erythrocyte and tetrameric brain AChE must undergo different postsynthetic modifications leading to differences in their glycosylation patterns. 相似文献
102.
Abstract The effect of spermine on the A-DNA to B-DNA transition in d(CGCGAATTCGCG)2 has been investigated by five A-start molecular dynamics simulations, using the Cornell et al. potential. In the absence of spermine an A→B transition is initiated immediately and the DNA becomes equidistant from the A- and B-forms at 200ps. In three DNA-spermine simulations, when a spermine is located across the major groove of A-DNA in one of three different initial locations, the time taken to reach equidistance from the A- and B-forms is delayed until 800, 950 or 1000ps. In each case the A-form appears to be temporarily stabilized by spermine's electrostatic interactions with phosphates on both sides of the major groove. The onset of the A→B transition can be correlated with the spermine losing contact with phosphates on one side of the groove and with A-like → B-like sugar pucker transitions in the vicinity of the spermine bridge. However in the fifth trajectory, in which the spermine initially threads from the major groove via the backbone into the minor groove, the B→A transition occurs rapidly once again and the DNA is equidistant between the A- and B-forms within 300ps. This indicates that the mere presence of spermine is insufficient to delay the transition and that major groove binding stabilizes A-DNA. 相似文献
103.
Harald Greger 《Biochemical Systematics and Ecology》1978,6(1):11-17
Leaf flavonoids of 13 Anacyclus taxa have been identified and compared. The most common compounds are 3-, 7- or 5-glycosylated flavonols which, together with the accumulation of 2 diosmetin 7-glycosides, help to delimitate species groups according to recent morphological and cytological findings. In addition to quercetagetin, quercetagetin 3'-methyl ether, patuletin and spinacetin have been isolated as 7-glucosides from the yellow disc and ray flowers of Anacyclus radiatus. The distribution patterns of polyacetylenes and particularly related amides, characterize different Anacyclus species and apparently contribute to a more natural interpretation of relationships with other genera, which may also be underlined by the distribution of cyanogenic glycosides. 相似文献
104.
Haijun Bin Indunil Angunawela Beibei Qiu Fallon J. M. Colberts Mengmeng Li Matthew J. Dyson Martijn M. Wienk Harald Ade Yongfang Li Ren A. J. Janssen 《Liver Transplantation》2020,10(34)
Compared to conjugated polymers, small‐molecule organic semiconductors present negligible batch‐to‐batch variations, but presently provide comparatively low power conversion efficiencies (PCEs) in small‐molecular organic solar cells (SM‐OSCs), mainly due to suboptimal nanomorphology. Achieving precise control of the nanomorphology remains challenging. Here, two new small‐molecular donors H13 and H14 , created by fluorine and chlorine substitution of the original donor molecule H11 , are presented that exhibit a similar or higher degree of crystallinity/aggregation and improved open‐circuit voltage with IDIC‐4F as acceptor. Due to kinetic and thermodynamic reasons, H13 ‐based blend films possess relatively unfavorable molecular packing and morphology. In contrast, annealed H14 ‐based blends exhibit favorable characteristics, i.e., the highest degree of aggregation with the smallest paracrystalline π–π distortions and a nanomorphology with relatively pure domains, all of which enable generating and collecting charges more efficiently. As a result, blends with H13 give a similar PCE (10.3%) as those made with H11 (10.4%), while annealed H14 ‐based SM‐OSCs have a significantly higher PCE (12.1%). Presently this represents the highest efficiency for SM‐OSCs using IDIC‐4F as acceptor. The results demonstrate that precise control of phase separation can be achieved by fine‐tuning the molecular structure and film formation conditions, improving PCE and providing guidance for morphology design. 相似文献
105.
Akchheta Karki Joachim Vollbrecht Alexander J. Gillett Philipp Selter Jaewon Lee Zhengxing Peng Nora Schopp Alana L. Dixon Max Schrock Vojtech Ndady Franz Schauer Harald Ade Bradley F. Chmelka Guillermo C. Bazan Richard H. Friend Thuc‐Quyen Nguyen 《Liver Transplantation》2020,10(29)
Even though significant breakthroughs with over 18% power conversion efficiencies (PCEs) in polymer:non‐fullerene acceptor (NFA) bulk heterojunction organic solar cells (OSCs) have been achieved, not many studies have focused on acquiring a comprehensive understanding of the underlying mechanisms governing these systems. This is because it can be challenging to delineate device photophysics in polymer:NFA blends comprehensively, and even more complicated to trace the origins of the differences in device photophysics to the subtle differences in energetics and morphology. Here, a systematic study of a series of polymer:NFA blends is conducted to unify and correlate the cumulative effects of i) voltage losses, ii) charge generation efficiencies, iii) non‐geminate recombination and extraction dynamics, and iv) nuanced morphological differences with device performances. Most importantly, a deconvolution of the major loss processes in polymer:NFA blends and their connections to the complex BHJ morphology and energetics are established. An extension to advanced morphological techniques, such as solid‐state NMR (for atomic level insights on the local ordering and donor:acceptor π? π interactions) and resonant soft X‐ray scattering (for donor and acceptor interfacial area and domain spacings), provide detailed insights on how efficient charge generation, transport, and extraction processes can outweigh increased voltage losses to yield high PCEs. 相似文献
106.
107.
108.
109.
110.
Blanca Domenech‐Ximenos Victor Cuba Pepus Daunis‐i‐Estadella Santiago Thi‐Henestrosa Francisco Jaldo Carles Biarnes Xavier Molina Gemma Xifra Wifredo Ricart Anton Bardera Imma Boada Marco Essig Salvador Pedraza Massimo Federici Jos Manuel Fernndez‐Real Josep Puig 《Obesity (Silver Spring, Md.)》2020,28(9):1663-1670