The role of seasonality in lettuce consumption: a case study of environmental and social aspects

Background, aim and scope  Considerable debate surrounds the assessment of the environmental impacts and the ethical justification for providing a year-round supply of fresh produce to consumers in the developed countries of northern Europe. Society is seeking environmentally sustainable supply chains which maintain the variety of fresh food on offer throughout the year. This paper compares the environmental impacts of different supply chains providing lettuce all year round to the UK and considers consumers' meanings of—and attitudes to—available options. Lettuce has been selected as a case study as its consumption has grown steadily during the last two decades and the supply chains through cold months are protected cropping in the UK and field cropping in Spain; during warm months, lettuce is sourced from field cropping in the UK. Materials and methods  Data were collected from farms supplying each of these supply chains, and life cycle assessment methodology was used to analyse a range of impacts associated with producing (from plant propagation to harvesting and post-harvest cooling) and delivering 1 kg of lettuce to a UK Regional Distribution Centre (RDC). The downstream stages (i.e. retailing, consumption and waste management) are the same regardless of the origin of the product and were omitted from the comparison. The impacts considered included potential to induce global warming and acidification as well as three inventory indicators (primary energy use, land use and water use). Qualitative data were collected in order to assess the consumer considerations of purchasing lettuce also during winter. Results  Importation of Spanish field-grown lettuce into the UK during winter produced fewer greenhouse gas (GHG) emissions than lettuce produced in UK-protected systems at that time (0.4–0.5 vs. 1.5–3.7 kg CO₂-eq/kg lettuce in RDC). Refrigerated transport to the UK was an important element of the global warming potential associated with Spanish lettuce (42.5% of emissions), whilst energy for heating dominated the results in UK-protected cultivation (84.3% of emissions). Results for acidification were more variable and no overall trends are apparent. Results from qualitative social analysis revealed complex and multidimensional meanings of freshness and suggested that the most striking seasonal variation in vegetable/salad eating was a tendency to consume more salads in the summer and more cooked vegetables in the winter, thus suggesting that in-home consumption alone cannot explain the rise in winter imports of lettuce to the UK. Discussion  UK field-grown lettuce had the lowest overall environmental impact; however, those lettuces are only available in summer, so consumers therefore need to either accept the environmental impacts associated with eating lettuce in the winter or to switch consumption to another food product in the winter. When lettuces were field-grown in Spain and then transported by road to the UK, the overall impacts were similar to the UK field lettuces. The variation within farms of the same country employing different cultivation regimes and practices was bigger than between farms of different countries. Conclusions  This paper has explored the environmental consequences of consuming lettuce year-round in the UK. Whilst recognising the small sample size, the comparative analysis of the different supply chains does suggest that seasonality can be an important variable when defining the best choice of lettuce from an environmental point of view. Recommendations and perspectives  Further studies considering more production sites and product types are required to obtain conclusions whose general validity is clear and for different types of fresh produce. A clear distinction to be made in such studies is whether crops are produced in open fields or under protection. New characterisation methods are needed for environmental impacts derived from the use of key agricultural resources such as land and water. Social studies to investigate consumer preferences and the possibility of moving to more seasonal diets should be an integral part of these studies using samples composed of both urban and rural consumers and using a mixed methodology with both quantitative and qualitative components.

The effects of acute treatment with Δ9-THC on exploratory behaviour and memory in the rat

The aim of the present study was to evaluate the effects of delta9-tetrahydrocannabinol (delta9-THC) on exploratory behaviour and memory, independent of its locomotor suppressive effects. Dopamine (DA) and noradrenaline (NA) contents were determined in the areas of the brain directly related to such behaviours (hippocampus, striatum and amygdala). An acute dose of delta9-THC led to a decrease in exploratory parameters and motor activity during the holeboard test. The radial arm maze was used to evaluate the effects of this cannabinoid substance on memory. Animals treated with delta9-THC committed more errors in the maze test compared to control, particularly when the retention process was put to test. Furthermore, treatment with delta9-THC led to reduced NA contents in the hippocampus and increased DA contents in the amygdala, without changes in the striatum.     

Assessing freshwater use impacts in LCA: Part I—inventory modelling and characterisation factors for the main impact pathways

Background, aim and scope

Freshwater is a basic resource for humans; however, its link to human health is seldom related to lack of physical access to sufficient freshwater, but rather to poor distribution and access to safe water supplies. On the other hand, freshwater availability for aquatic ecosystems is often reduced due to competition with human uses, potentially leading to impacts on ecosystem quality. This paper summarises how this specific resource use can be dealt with in life cycle analysis (LCA).

Main features

The main quantifiable impact pathways linking freshwater use to the available supply are identified, leading to definition of the flows requiring quantification in the life cycle inventory (LCI).

Results

The LCI needs to distinguish between and quantify evaporative and non-evaporative uses of ‘blue’ and ‘green’ water, along with land use changes leading to changes in the availability of freshwater. Suitable indicators are suggested for the two main impact pathways [namely freshwater ecosystem impact (FEI) and freshwater depletion (FD)], and operational characterisation factors are provided for a range of countries and situations. For FEI, indicators relating current freshwater use to the available freshwater resources (with and without specific consideration of water ecosystem requirements) are suggested. For FD, the parameters required for evaluation of the commonly used abiotic depletion potentials are explored.

Discussion

An important value judgement when dealing with water use impacts is the omission or consideration of non-evaporative uses of water as impacting ecosystems. We suggest considering only evaporative uses as a default procedure, although more precautionary approaches (e.g. an ‘Egalitarian’ approach) may also include non-evaporative uses. Variation in seasonal river flows is not captured in the approach suggested for FEI, even though abstractions during droughts may have dramatic consequences for ecosystems; this has been considered beyond the scope of LCA.

Conclusions

The approach suggested here improves the representation of impacts associated with freshwater use in LCA. The information required by the approach is generally available to LCA practitioners

Recommendations and perspectives

The widespread use of the approach suggested here will require some development (and consensus) by LCI database developers. Linking the suggested midpoint indicators for FEI to a damage approach will require further analysis of the relationship between FEI indicators and ecosystem health.     

Biomechanic consequences of differences in wing morphology betweenTadarida brasiliensis andMyotis chiloensis

The wing morphology of bats is very diverse, and may correlate with energetic, behavioural, and ecological demands. If these demands conflict, wing shape may reflect compromise solutions. In this study, we compared the wing morphology of two bats,Tadarida brasiliensis (Geoffroy, 1824) andMyotis chiloensis (Waterhouse, 1828), that differ in body size, habitat, and foraging behaviour. We analyzed features of biomechanical and energetic relevance, and sought evidence of compromise solutions to energetic, ecological, and behavioural demands. We found that wing span of both species conformed to expectations based on allometric relationships, but that although the wing area ofM. chiloensis did not differ from predictions, the wing area ofT. brasiliensis was lower.M. chiloensis possessed an unusually low second moment of area of the humerus. Wing form ofM. chiloensis is consistent with highly maneuverable flight needed to live between shrubs and wooded habitats, and its low aspect ratio and low wing loading indicate a high energetic cost and a low flight speed, respectively. The low humeral second moment of area may be related to a reduction of wing mass and may result in decreased inertial power. In contrast,T. brasiliensis showed high aspect ratio and wing loading, characteristic of high speed, energetically economic flight.     

Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective alpha(1b)-adrenoceptor mutant demonstrates membrane targeting of GPCR oligomers

Many G-protein-coupled receptors, including the alpha(1b)-adrenoceptor, form homo-dimers or oligomers. Mutation of hydrophobic residues in transmembrane domains I and IV alters the organization of the alpha(1b)-adrenoceptor oligomer, with transmembrane domain IV playing a critical role. These mutations also result in endoplasmic reticulum trapping of the receptor. Following stable expression of this alpha(1b)-adrenoceptor mutant, cell surface delivery, receptor function and structural organization were recovered by treatment with a range of alpha(1b)-adrenoceptor antagonists that acted at the level of the endoplasmic reticulum. This was accompanied by maturation of the mutant receptor to a terminally N-glycosylated form, and only this mature form was trafficked to the cell surface. Co-expression of the mutant receptor with an otherwise wild-type form of the alpha(1b)-adrenoceptor that is unable to bind ligands resulted in this wild-type variant also being retained in the endoplasmic reticulum. Ligand-induced cell surface delivery of the mutant alpha(1b)-adrenoceptor now allowed co-recovery to the plasma membrane of the ligand-binding-deficient mutant. These results demonstrate that interactions between alpha(1b)-adrenoceptor monomers occur at an early stage in protein synthesis, that ligands of the alpha(1b)-adrenoceptor can act as pharmacological chaperones to allow a structurally compromised form of the receptor to pass cellular quality control, that the mutated receptor is not inherently deficient in function and that an oligomeric assembly of ligand-binding-competent and -incompetent forms of the alpha(1b)-adrenoceptor can be trafficked to the cell surface by binding of a ligand to only one component of the receptor oligomer.     

Assessing freshwater use impacts in LCA,part 2: case study of broccoli production in the UK and Spain

Background, aim and scope  

Milà i Canals et al. (Int J Life Cycle Ass 14(1):28-42, 2009) referred to as ‘Part 1’ in this paper) showed that impacts associated with use of freshwater must be treated more rigorously than is usual in life cycle assessment (LCA), going beyond the conventional consideration only of ‘blue’ water (i.e. irrigation and other abstractions), and suggested an operational method to include the impacts on freshwater ecosystems (freshwater ecosystem impact) and abiotic resource depletion (freshwater depletion). The inclusion of water-related impacts in LCA is of paramount importance, particularly for agricultural systems due to their large water consumption worldwide. A case study of UK consumption of broccoli grown in the UK and Spain is presented here to illustrate the method suggested in Part 1.     

2-aminohydroxamic acid derivatives as inhibitors of Bacillus cereus phosphatidylcholine preferred phospholipase C PC-PLC(Bc)

Phosphatidylcholine preferring phospholipase C (PC-PLC) is an important enzyme that plays a key role in a variety of cellular events and lipid homoeostases. Bacillus cereus phospholipase C (PC-PLC(Bc)) has antigenic similarity with the elusive mammalian PC-PLC, which has not thus far been isolated and purified. Therefore the discovery of inhibitors of PC-PLC(Bc) is of current interest. Here, we describe the synthesis and biological evaluation of a new type of compounds inhibiting PC-PLC(Bc). These compounds have been designed by evolution of previously described 2-aminohydroxamic acid PC-PLC(Bc) inhibitors that block the enzyme by coordination of the zinc active site atoms present in PC-PLC(Bc) [Gonzalez-Roura, A.; Navarro, I.; Delgado, A.; Llebaria, A.; Casas, J. Angew. Chem. Int. Ed.2004, 43, 862]. The new compounds maintain the zinc coordinating groups and possess an extra trimethylammonium function, linked to the hydroxyamide nitrogen by an alkyl chain, which is expected to mimic the trimethylammonium group of the phosphatidylcholine PC-PLC(Bc) substrates. Some of the compounds described inhibit the enzyme with IC(50)'s in the low micromolar range. Unexpectedly, the most potent inhibitors found are those that possess a trimethylammonium group but have chemically blocked the zinc coordinating functionalities. The results obtained suggest that PC-PLC(Bc) inhibition is not due to the interaction of compounds with the phospholipase catalytic zinc atoms, but rather results from the inhibitor cationic group recognition by the PC-PLC(Bc) amino acids involved in choline lipid binding.     

Regulated Secretion of Acid Sphingomyelinase: IMPLICATIONS FOR SELECTIVITY OF CERAMIDE FORMATION*

The acid sphingomyelinase (aSMase) gene gives rise to two distinct enzymes, lysosomal sphingomyelinase (L-SMase) and secretory sphingomyelinase (S-SMase), via differential trafficking of a common protein precursor. However, the regulation of S-SMase and its role in cytokine-induced ceramide formation remain ill defined. To determine the role of S-SMase in cellular sphingolipid metabolism, MCF7 breast carcinoma cells stably transfected with V5-aSMase^WT were treated with inflammatory cytokines. Interleukin-1β and tumor necrosis factor-α induced a time- and dose-dependent increase in S-SMase secretion and activity, coincident with selective elevations in cellular C₁₆-ceramide. To establish a role for S-SMase, we utilized a mutant of aSMase (S508A) that is shown to retain L-SMase activity, but is defective in secretion. MCF7 expressing V5-aSMase^WT exhibited increased S-SMase and L-SMase activity, as well as elevated cellular levels of specific long-chain and very long-chain ceramide species relative to vector control MCF7. Interestingly, elevated levels of only certain very long-chain ceramides were evident in V5-aSMase^S508A MCF7. Secretion of the S508A mutant was also defective in response to IL-1β, as was the regulated generation of C₁₆-ceramide. Taken together, these data support a crucial role for Ser⁵⁰⁸ in the regulation of S-SMase secretion, and they suggest distinct metabolic roles for S-SMase and L-SMase.     

LCA mainstreaming conditions in Latin America—based on learnings from 2005 to 2014

Purpose

Based on the 2005–2014 developments in the Latin American and the Caribbean region (LAC), this paper aims to understand the conditions’ levels for mainstreaming life cycle assessment/life cycle management (LCA/LCM) and map key next actions.

Methods

Along the paper, four mainstreaming conditions are analyzed: expansion of LCM/LCA training activities, availability of LCA studies, national LCA database operating, and existence and activity of national life cycle network(s). Assuming that countries with better conditions are in a better position to develop national LCA based regulations, policies are also researched to complement this study.

Results and discussion

With nine life cycle (LC) networks in 2014, the LAC region has positively developed its networking capacities since 2005 but not the databases area (only one LCA database, Mexicaniuh, is fully operational). It was found that countries with no networks, lack all LCA trainings, studies, and databases.Local capacities are limited which in best case, Chile, does not exceed 18 practitioners per 10 million inhabitants. Based on the total score on mainstreaming conditions, Mexico and Brazil are the most advanced countries, but their markets for LCA professionals are still small (Valdivia et al. 2015), which suggests that tailored made strategies are needed for stronger uptake of LCA by industrial sectors.Argentina, Peru, Chile, and Colombia are in the second tier but still lack a critical mass of business cases and the political will to improve their mainstreaming conditions.

Conclusions

LCA development in the LAC region since 2005 is overall positive but still insufficient to serve the growth of prosperous LCA markets. Well-functioning LC networks are essential to leapfrog LCA. In 2014, about 27 % of LAC countries counted on a LC network. A common language in the region (except for Portuguese in Brazil) has been instrumental for expanding LCA through regional cooperation. LCA-based policies are boosted when local capacities and databases are available following the cases of Mexico, Chile, and Brazil. More data and research are needed to understand the women role in advancing LCA and the causalities and motivations of LAC companies to decide for LCA implementation. The application of the methodology was possible thanks to good quality data available and delivered key findings to develop national road maps for advancing LCA. No indicator used is specific for the LAC region and similar exercises are encouraged in other regions such as Africa and Asia.