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431.
Acute Alterations of Glutamate,Glutamine, GABA,and Other Amino Acids After Spinal Cord Contusion in Rats 总被引:1,自引:0,他引:1
Diaz-Ruiz A Salgado-Ceballos H Montes S Maldonado V Tristan L Alcaraz-Zubeldia M Ríos C 《Neurochemical research》2007,32(1):57-63
Spinal cord injury (SCI) leads to an alteration of energetic metabolism. As a consequence, glutamate, glutamine, aspartate
and other important amino acids are altered after damage, leading to important disregulation of the neurochemical pathways.
In the present study, we characterized the acute-phase changes in tissue concentration of amino acids involved in neurotransmitter
and non-neurotransmitter actions after SCI by contusion in rats. Animals were submitted to either laminectomy or SCI by contusion
and sacrificed at 2, 4, 8, and 12 h after lesion, for the analysis of tissue amino acids by HPLC. Results showed that both
aspartate and glutamate contents diminished after SCI, while glutamine concentrations raised, however, the sum of molar concentrations
of glutamate plus glutamine remained unchanged at all time points. GABA concentrations increased versus control group, while
glycine remained unchanged. Finally, citrulline levels increased by effect of SCI, while taurine-increased only 4 h after
lesion. Results indicate complex acute-phase changes in amino acids concentrations after SCI, reflecting the different damaging
processes unchained after lesion. 相似文献
432.
Carboxylic acids such as citric, lactic, succinic and itaconic acids are useful products and are obtained on large scale by fermentation. This review describes the options for recovering these and other fermentative carboxylic acids. After cell removal, often a primary recovery step is performed, using liquid–liquid extraction, adsorption, precipitation or conventional electrodialysis. If the carboxylate is formed rather than the carboxylic acid, the recovery process involves a step for removing the cation of the formed carboxylate. Then, bipolar electrodialysis and thermal methods for salt splitting can prevent that waste inorganic salts are co-produced. Final carboxylic acid purification requires either distillation or crystallization, usually involving evaporation of water. 相似文献
433.
Melanie McFarlane Camilo Arias-Goeta Estelle Martin Zoe O'Hara Aleksei Lulla Laurence Mousson Stephanie M. Rainey Suzana Misbah Esther Schnettler Claire L. Donald Andres Merits Alain Kohl Anna-Bella Failloux 《PLoS neglected tropical diseases》2014,8(7)
Replication of arboviruses in their arthropod vectors is controlled by innate immune responses. The RNA sequence-specific break down mechanism, RNA interference (RNAi), has been shown to be an important innate antiviral response in mosquitoes. In addition, immune signaling pathways have been reported to mediate arbovirus infections in mosquitoes; namely the JAK/STAT, immune deficiency (IMD) and Toll pathways. Very little is known about these pathways in response to chikungunya virus (CHIKV) infection, a mosquito-borne alphavirus (Togaviridae) transmitted by aedine species to humans resulting in a febrile and arthralgic disease. In this study, the contribution of several innate immune responses to control CHIKV replication was investigated. In vitro experiments identified the RNAi pathway as a key antiviral pathway. CHIKV was shown to repress the activity of the Toll signaling pathway in vitro but neither JAK/STAT, IMD nor Toll pathways were found to mediate antiviral activities. In vivo data further confirmed our in vitro identification of the vital role of RNAi in antiviral defence. Taken together these results indicate a complex interaction between CHIKV replication and mosquito innate immune responses and demonstrate similarities as well as differences in the control of alphaviruses and other arboviruses by mosquito immune pathways. 相似文献
434.
Central nervous system (CNS) diseases are difficult to treat because of the blood-brain barrier (BBB), which prevents most drugs from entering into the brain. Intranasal (IN) administration is a promising approach for drug delivery to the brain, bypassing the BBB; however, its application has been restricted to particularly potent substances and it does not offer localized delivery to specific brain sites. Focused ultrasound (FUS) in combination with microbubbles can deliver drugs to the brain at targeted locations. The present study proposed to combine these two different platform techniques (FUS+IN) for enhancing the delivery efficiency of intranasally administered drugs at a targeted location. After IN administration of 40 kDa fluorescently-labeled dextran as the model drug, FUS targeted at one region within the caudate putamen of mouse brains was applied in the presence of systemically administered microbubbles. To compare with the conventional FUS technique, in which intravenous (IV) drug injection is employed, FUS was also applied after IV injection of the same amount of dextran in another group of mice. Dextran delivery outcomes were evaluated using fluorescence imaging of brain slices. The results showed that FUS+IN enhanced drug delivery within the targeted region compared with that achieved by IN only. Despite the fact that the IN route has limited drug absorption across the nasal mucosa, the delivery efficiency of FUS+IN was not significantly different from that of FUS+IV. As a new drug delivery platform, the FUS+IN technique is potentially useful for treating CNS diseases. 相似文献
435.
Gabriel M. Moulatlet Flávia R. C. Costa Camilo D. Rennó Thaise Emilio Juliana Schietti 《Biotropica》2014,46(4):395-403
Amazonian forests harbor a large variety of understory herbs adapted to areas with different hydrological conditions, ranging from well‐drained to seasonally flooded forests. The presence versus absence of flooding forms the extremes of a hydrological gradient, with various intermediate conditions, such as seasonal soil waterlogged areas, in between. We investigated the relationship between understory herbs and hydrological conditions in Central Amazonian forests using eighty‐eight 250 × 2 m plots distributed along a 600‐km transect. Hydrological conditions were determined regionally by precipitation and locally by topographic conditions based on drainage potential, flooding height and soil permeability (sand content). Soil cation concentration was used as a proxy for soil fertility. The floristic dissimilarities among plots were visualized by Nonmetric Multidimensional Scaling, and simple and multiple regressions were used to identify the best predictor of herb species composition. Local drainage potential was more important in determining herb species composition than soil fertility or precipitation at non‐flooded and flooded sites. Flooded sites comprised a very distinctive herb species composition even when the flood height was low (0.3 m). We conclude that hydrological conditions are the primary constraint of herb distribution within this flat regional landscape with moderate amounts of soil fertility variation (0.09–2.280 cmol(+)/kg). Hydrological models that consider local water conditions explained the largest part of herb species composition. Therefore, predictions of species distribution based on large‐scale climatic variables may underestimate the favorable area for understory herbs if the variation on local hydrological conditions is not considered. 相似文献
436.
Maria C. Melo Camilo Salazar Chris D. Jiggins Mauricio Linares 《Evolution; international journal of organic evolution》2009,63(6):1660-1665
Homoploid speciation generates species without a change in chromosome number via introgressive hybridization and has been considered rare in animals. Heliconius butterflies exhibit bright aposematic color patterns that also act as cues in assortative mating. Heliconius heurippa has a color pattern that can be recreated by introgression of the H. melpomene red band into an H. cydno genetic background. Wild H. heurippa males show assortative mating based on color pattern and we here investigate the origin of this preference by studying first-generation backcross hybrids between H. melpomene and H. cydno that resemble H. heurippa . These hybrids show assortative mating preferences, showing a strong preference for their own color pattern over that of either parental species. This is consistent with a genetic basis to wing pattern preference and implies, first, that assortative mating preferences would facilitate the initial establishment of a homozygous hybrid color pattern by increasing the likelihood that early generation hybrids mate among themselves. Second, once established such a lineage would inherit assortative mating preferences that would lead to partial reproductive isolation from parental lineages. 相似文献
437.
Camilo L. Medina Maria Cristina Sanches Maria Luiza S. Tucci Carlos A. F. Sousa Geraldo Rog��rio F. Cuzzuol Carlos A. Joly 《Annals of botany》2009,104(4):671-680
Background and Aims
Erythrina speciosa is a Neotropical tree that grows mainly in moist habitats. To characterize the physiological, morphological and growth responses to soil water saturation, young plants of E. speciosa were subjected experimentally to soil flooding.Methods
Flooding was imposed from 2 to 4 cm above the soil surface in water-filled tanks for 60 d. Non-flooded (control) plants were well watered, but never flooded. The net CO2 exchange (ACO2), stomatal conductance (gs) and intercellular CO2 concentration (Ci) were assessed for 60 d. Soluble sugar and free amino acid concentrations and the proportion of free amino acids were determined at 0, 7, 10, 21, 28 and 45 d of treatments. After 28, 45 and 60 d, dry masses of leaves, stems and roots were determined. Stem and root cross-sections were viewed using light microscopy.Key Results
The ACO2 and gs were severely reduced by flooding treatment, but only for the first 10 d. The soluble sugars and free amino acids increased until the tenth day but decreased subsequently. The content of asparagine in the roots showed a drastic decrease while those of alanine and γ-aminobutyric increased sharply throughout the first 10 d after flooding. From the 20th day on, the flooded plants reached ACO2 and gs values similar to those observed for non-flooded plants. These events were coupled with the development of lenticels, adventitious roots and aerenchyma tissue of honeycomb type. Flooding reduced the growth rate and altered carbon allocation. The biomass allocated to the stem was higher and the root mass ratio was lower for flooded plants when compared with non-flooded plants.Conclusions
Erythrina speciosa showed 100 % survival until the 60th day of flooding and was able to recover its metabolism. The recovery during soil flooding seems to be associated with morphological alterations, such as development of hypertrophic lenticels, adventitious roots and aerenchyma tissue, and with the maintenance of neutral amino acids in roots under long-term exposure to root-zone O2 deprivation.Key words: Erythrina speciosa, aerenchyma, amino acid content, biomass allocation, photosynthesis, flooding adaptations, stomatal conductance, O2 deficiency, γ-aminobutyric acid (GABA) 相似文献438.
Zeni Jaquelini O. Hoeinghaus David J. Roa-Fuentes Camilo A. Casatti Lilian 《Hydrobiologia》2020,847(18):3829-3843
Hydrobiologia - Beta diversity quantifies changes in assemblages among sites and can identify how anthropogenic changes affect species distributions patterns. We investigated how spatial and... 相似文献
439.
López-Aguirre Camilo Hand Suzanne J. Simmons Nancy B. Silcox Mary T. 《Journal of Mammalian Evolution》2022,29(3):531-545
Journal of Mammalian Evolution - Diet has been linked to the diversification of the bat superfamily Noctilionoidea, a group that underwent an impressive ecological diversification within Mammalia.... 相似文献
440.