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61.
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Camilla Esberg Ben du Toit Rickard Olsson Ulrik Ilstedt Reiner Giesler 《Plant and Soil》2010,329(1-2):209-225
Forests growing on highly weathered soils are often phosphorus (P) limited and competition between geochemical and biological sinks affects their soil P dynamics. In an attempt to elucidate the factors controlling the relative importance of these two sinks, we investigated the relationship of between soil microbial growth kinetics and soil chemical properties following amendments with C, N and P in six South African forest soils. Microbial growth kinetics were determined from respiration curves derived from measurements of CO2 effluxes from soil samples in laboratory incubations. We found that microbial growth rates after C + N additions were positively related to NaOH-extractable P and decreased with soil depth, whereas the lag time (the time between substrate addition and exponential growth) was negatively related to extractable P. However, the growth rate and lag time were unrelated to the soil’s sorption properties or Al and Fe contents. Our results indicate that at least some of the NaOH-extractable inorganic P may be biologically available within a relatively short time (days to weeks) and might be more labile than previously thought. Our results also show that microbial utilization of C + N only seemed to be constrained by P in the deeper part of the soil profiles. 相似文献
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Metallic nanoparticles of gold functionalized with oligonucleotides conventionally use a terminal thiol modification and have been used in a wide range of applications. Although readily available, the oligonucleotide–nanoparticle conjugates prepared in this way suffer from a lack of stability when exposed to a variety of small molecules or elevated temperatures. If silver is used in place of gold then this lack of stability is even more pronounced. In this study we report the synthesis of highly stabilized oligonucleotide–nanoparticle conjugates using a simple oligonucleotide modification. A modified solid support was used to generate 3′-thioctic acid modified oligonucleotides by treatment with an N-hydroxysuccimidyl ester of thioctic acid. Unusually, both gold and silver nanoparticles have been investigated in this study and show that these disulphide-modified oligonucleotide probes offer significant improvements in nanoparticle stability when treated with dithiothreitol (DTT) compared with monothiol analogues. This is a significant advance in oligonucleotide–nanoparticle conjugate stability and for the first time allows silver nanoparticles to be prepared that are more stable than standard gold-thiol functionalized nanoparticles. This opens up the possibility of using silver nanoparticles functionalized with oligonucleotides as an alternative to gold. 相似文献
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Ward Delphi Melbourne-Thomas Jessica Pecl Gretta T. Evans Karen Green Madeline McCormack Phillipa C. Novaglio Camilla Trebilco Rowan Bax Narissa Brasier Madeleine J. Cavan Emma L. Edgar Graham Hunt Heather L. Jansen Jan Jones Russ Lea Mary-Anne Makomere Reuben Mull Chris Semmens Jayson M. Shaw Janette Tinch Dugald van Steveninck Tatiana J. Layton Cayne 《Reviews in Fish Biology and Fisheries》2022,32(1):65-100
Reviews in Fish Biology and Fisheries - Marine ecosystems and their associated biodiversity sustain life on Earth and hold intrinsic value. Critical marine ecosystem services include maintenance of... 相似文献
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Bax Narissa Novaglio Camilla Maxwell Kimberley H. Meyers Koen McCann Joy Jennings Sarah Frusher Stewart Fulton Elizabeth A. Nursey-Bray Melissa Fischer Mibu Anderson Kelli Layton Cayne Emad Gholam Reza Alexander Karen A. Rousseau Yannick Lunn Zau Carter Chris G. 《Reviews in Fish Biology and Fisheries》2022,32(1):189-207
Reviews in Fish Biology and Fisheries - Humans have relied on coastal resources for centuries. However, current growth in population and increased accessibility of coastal resources through... 相似文献
68.
Endosomes are known to undergo budding and fission reactions that separate regions destined for lysosomal degradation from carriers to be recycled to the plasma membrane. A recent paper (Rowland et al, 2014 ) shows that contact sites between endosomes and the endoplasmic reticulum (ER) define the position and timing for fission. This uncovers an unanticipated role for the ER in controlling endosomal sorting and maturation. 相似文献
69.
Zhang Yang Catharina Steentoft Camilla Hauge Lars Hansen Allan Lind Thomsen Francesco Niola Malene B. Vester-Christensen Morten Fr?din Henrik Clausen Hans H. Wandall Eric P. Bennett 《Nucleic acids research》2015,43(9):e59
The nuclease-based gene editing tools are rapidly transforming capabilities for altering the genome of cells and organisms with great precision and in high throughput studies. A major limitation in application of precise gene editing lies in lack of sensitive and fast methods to detect and characterize the induced DNA changes. Precise gene editing induces double-stranded DNA breaks that are repaired by error-prone non-homologous end joining leading to introduction of insertions and deletions (indels) at the target site. These indels are often small and difficult and laborious to detect by traditional methods. Here we present a method for fast, sensitive and simple indel detection that accurately defines indel sizes down to ±1 bp. The method coined IDAA for Indel Detection by Amplicon Analysis is based on tri-primer amplicon labelling and DNA capillary electrophoresis detection, and IDAA is amenable for high throughput analysis. 相似文献
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