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71.
Ana Paula Ribeiro Silvia Maria Amado Jo?o Roberto Casanova Dinato Vitor Daniel Tessutti Isabel Camargo Neves Sacco 《PloS one》2015,10(9)
Aim/Hypothesis
The etiology of plantar fasciitis (PF) has been related to several risk factors, but the magnitude of the plantar load is the most commonly described factor. Although PF is the third most-common injury in runners, only two studies have investigated this factor in runners, and their results are still inconclusive regarding the injury stage.Objective
Analyze and compare the plantar loads and vertical loading rate during running of runners in the acute stage of PF to those in the chronic stage of the injury in relation to healthy runners.Methods
Forty-five runners with unilateral PF (30 acute and 15 chronic) and 30 healthy control runners were evaluated while running at 12 km/h for 40 meters wearing standardized running shoes and Pedar-X insoles. The contact area and time, maximum force, and force-time integral over the rearfoot, midfoot, and forefoot were recorded and the loading rate (20–80% of the first vertical peak) was calculated. Groups were compared by ANOVAs (p<0.05).Results
Maximum force and force-time integral over the rearfoot and the loading rate was higher in runners with PF (acute and chronic) compared with controls (p<0.01). Runners with PF in the acute stage showed lower loading rate and maximum force over the rearfoot compared to runners in the chronic stage (p<0.01).Conclusion
Runners with PF showed different dynamic patterns of plantar loads during running over the rearfoot area depending on the injury stage (acute or chronic). In the acute stage of PF, runners presented lower loading rate and forces over the rearfoot, possibly due to dynamic mechanisms related to pain protection of the calcaneal area. 相似文献72.
Nícholas F. de Camargo Juliana F. Ribeiro Amabílio J. A. de Camargo Emerson M. Vieira 《Acta theriologica》2014,59(1):183-191
Investigation of the effect of endogenous and exogenous factors on the diet of animals is necessary for a better understanding of their feeding habits. This approach can provide relevant information on the autoecology of a species and its ecological interactions. We investigated the composition and intraspecific variation in the diet of the marsupial Gracilinanus agilis in areas of dry woodland forests (i.e., cerradão) in the Cerrado of Central Brazil, taking into consideration the availability of prey (arthropods) in the environment. We found insects, spiders, birds, and fruits in the scats of G. agilis. Insects (orders Hymenoptera, Isoptera, Hemiptera, and Coleoptera) and fruits were the most frequently consumed resources. Males fed more heavily on insects than females did, whereas during the warm–wet season (October to April), the reproductive females fed on insects more than the nonreproductive females did. On the other hand, the consumption of fruits and vertebrates did not vary between seasons, sexes, or according to female reproductive condition. Moreover, reproductive females fed more frequently on ants and beetles than nonreproductive females did. We also detected both positive (for Isoptera and Hemiptera) and negative (for Hymenoptera) selection of insects during the cool–dry season, whereas in the warm–wet season, these resources were consumed according to their availability in the environment. Our study revealed that G. agilis is an insectivore–omnivore species, but fruits also are a relevant part of its diet. This marsupial seemed to select their prey qualitatively according to its energy demands and nutritional requirements. 相似文献
73.
74.
Fabricio Fernandes Fontana Celso Tadeu Barbosa dos Santos Flavia Maria Esteves Ademir Rocha Geisa Ferreira Fernandes Cristiane Candida do Amaral Marcos Abel Domingues Zoilo Pires De Camargo Mario León Silva-Vergara 《Mycopathologia》2010,169(3):159-165
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban
and 126 rural dogs was carried out using ELISA and intradermal tests with the gp43 antigen of P. brasiliensis in Uberaba, Minas Gerais state of Brazil. Forty-one out of 149 urban dogs were euthanatized and had their lungs, liver and
spleen removed. One slice from each viscera was processed for histopathological examination and the remaining was homogenized and then cultivated on mycobiotic agar
at room temperature and Fava-Netto medium at 35°C and observed for 12 weeks. Of urban dogs, 75 (50.3%) were small adult females,
56 (36%) were strays, while 93 (64%) had been donated to the municipal zoonosis control center. Nine (6.2%) had a positive
intradermal test without statistical differences regarding gender, race, nutritional status or origin. No colonies with microscopic
or morphology appearances resembling P. brasiliensis were isolated, nor granulomatous process or fungal structures were observed from histopathological examination. Eighty (53.6%)
of the urban dogs presented seroreactivity, without statistical differences regarding gender, race, nutritional state, origin,
or positive intradermal test. Of 126 rural dogs, 102 (80.5%) presented antibodies against gp43 antigen, and this was statistically
significant in relation to the reactivity detected in urban dogs (P = 0.0001). Thus, dogs are commonly infected with P. brasiliensis, but they probably present natural resistance to develop paracoccidioidomycosis. 相似文献
75.
Patricia Fagundes Costa Geisa Ferreira Fernandes Priscila Oliveira dos Santos Cristiane Candida Amaral Zoilo Pires Camargo 《Mycopathologia》2010,169(1):37-46
The ecological niche or exact habitat of the fungus Paracoccidioides brasiliensis is not known, and few isolates have been obtained from the environment. In this study, ten isolates were analyzed with respect
to antigenic composition, serology, pathogenicity, and molecular aspects. Gp43 is considered to be the molecular basis for
the serodiagnosis of paracoccidioidomycosis; however, in this study only six of the environmental isolates secreted this molecule
(four in great amounts and two in small amounts). Other molecules were also produced. When exoantigens from these isolates
were tested using immunodiffusion, only four preparations were positive by ID tests. However, when these exoantigens were
tested by ELISA, all of them except one were able to detect anti-P. brasiliensis antibodies. In Western blot assays, these exoantigens showed different reactivities. Isolates that secreted gp43 presented
positive reactions for this molecule, and isolates that did not secrete gp43 gave positive reactions for other minor molecules.
RAPD analysis revealed that there is great genetic variation between these environmental isolates. These isolates were non-pathogenic:
no mortality was observed among the inoculated mice during an 18-month follow-up period. 相似文献
76.
77.
The ovary of the tick Amblyomma triste is classified as panoistic, which is characterized by the presence of oogonia without nurse and follicular cells. The present study has demonstrated that the oocytes in all developmental stages (I-IV) are attached to the ovary through a pedicel, a cellular structure that synthesizes and provides carbohydrate, lipids and proteins supplies for the oocytes during the vitellogenesis process. The lipids are deposited during all oocyte stages; they are freely distributed as observed in stages II, III and IV or they form complexes with other elements. The proteins are also deposited in all stages of the oocytes, however, in lower concentration in the stage IV. There is carbohydrate deposition from oocytes in the stage II as well as in stages III and IV. In addition, the present work has demonstrated that the oocyte yolk of A. triste has a glycolipoprotein nature and the elements are deposited in the following sequence: firstly the lipids and proteins, and finally the carbohydrates. 相似文献
78.
Populations of Dalbulus maidis (DeLong and Wolcott) from the northeastern and central-southern regions of Brazil differ morphologically, suggesting that they could be genetically isolated. Here we used the random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) technique to estimate genetic structuring of this leafhopper species among five geographically distant localities across those regions and to estimate gene flow between populations. Ten specimens were sampled per population and genotyped with RAPD markers generated from amplification with nine oligonucleotides. The percentage of polymorphic loci was 78% in relation to the total number of amplified loci, and genetic similarity either between or within populations was higher than 0.72. Cluster analysis grouped specimens from the northeastern population (Mossoró/RN) into a single group, whereas central-southern specimens were not grouped in relation to their places of origin. Overall, the genetic subdivision index (Fst) was low (or= 0.192 and Nm 相似文献
79.
Births were observed in two free-ranging groups of red-handed howlers (Alouatta belzebul) in eastern Brazilian Amazonia. Both events occurred in the early afternoon, and were relatively rapid and discrete. No other group members approached the females during parturition, nor attempted to interfere with the neonate in any way. Only one of the mothers ingested the placenta. This same female carried the neonate ventrally during the first month of life and then it was carried dorsally. The other infant was carried by its mother in a dorsal position from birth onward. Both infants survived their first month, but subsequently disappeared, in the second and fourth months of life. The precise causes of their death are unknown. In one case, we speculate that the infant's death was due to infestation by botfly larvae. 相似文献
80.
Pavanelli WR Kaminami MS Geres JR Sano A Ono MA Camargo IC Itano EN 《Mycopathologia》2007,163(3):117-128
Paracoccidioidomycosis (PCM) is a granulomatous disease caused by a dimorphic fungus, Paracoccidioides brasiliensis. The present study investigated the protective activity of the P. brasiliensis high-molecular-mass (hMM) fraction (~380 kDa) in experimental murine PCM. In the first step, lymphocyte proliferation and
production of IFNγ (but not IL-4) were observed in “in vitro” spleen cells (from female BALB/c mice infected (i.v.) with P. brasiliensis) that were stimulated with hMM fractions. In the second step, female BALB/c mice were previously immunized (s.c.) with hMM
fraction (25 μg/protein = F-25 and 50 μg/protein = F-50), and the colony-forming units (CFU) of the lung and spleen, the histopathological
characteristics of the granulomatous lesions, and plasmatic gp43 soluble antigens and anti-hMM IgG levels were analyzed at
28 and 56 days after infection. The lung and liver CFU were lower in mice previously immunized with the hMM fraction (P < 0.05). The granulomatous lesions revealed a greater degree of compaction and organization, with no dissemination of the
fungus to other organs. Lower soluble antigen levels (P < 0.05) and higher IgG anti-hMM fraction (P < 0.05) were observed in immunized groups. The results for CFU, histopathology and antigenemia suggest that the hMM fraction
has a protective effect in experimental paracoccidioidomycosis in BALB/c mice. 相似文献