全文获取类型
收费全文 | 179篇 |
免费 | 12篇 |
出版年
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 1篇 |
2018年 | 7篇 |
2017年 | 2篇 |
2016年 | 4篇 |
2015年 | 5篇 |
2014年 | 11篇 |
2013年 | 15篇 |
2012年 | 19篇 |
2011年 | 13篇 |
2010年 | 8篇 |
2009年 | 7篇 |
2008年 | 9篇 |
2007年 | 8篇 |
2006年 | 7篇 |
2005年 | 7篇 |
2004年 | 5篇 |
2003年 | 7篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 7篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1984年 | 3篇 |
1982年 | 1篇 |
1976年 | 2篇 |
1968年 | 1篇 |
排序方式: 共有191条查询结果,搜索用时 359 毫秒
101.
Francesco Iemolo Giovanni Duro Claudia Rizzo Laura Castiglia Vladimir Hachinski Calogero Caruso 《Immunity & ageing : I & A》2009,6(1):13-9
The concept of Vascular Dementia (VaD) has been recognized for over a century, but its definition and diagnostic criteria
remain unclear. 相似文献
102.
Armando Bartolazzi Calogero D'Alessandria Maria Gemma Parisella Alberto Signore Fabrizio Del Prete Luca Lavra Sten Braesch-Andersen Roberto Massari Carlo Trotta Alessandro Soluri Salvatore Sciacchitano Francesco Scopinaro 《PloS one》2008,3(11)
Background
The prevalence of thyroid nodules increases with age, average 4–7% for the U.S.A. adult population, but it is much higher (19–67%) when sub-clinical nodules are considered. About 90% of these lesions are benign and a reliable approach to their preoperative characterization is necessary. Unfortunately conventional thyroid scintigraphy does not allow the distinction among benign and malignant thyroid proliferations but it provides only functional information (cold or hot nodules).The expression of the anti-apoptotic molecule galectin-3 is restricted to cancer cells and this feature has potential diagnostic and therapeutic implications. We show here the possibility to obtain thyroid cancer imaging in vivo by targeting galectin-3.Methods
The galectin-3 based thyroid immuno-scintigraphy uses as radiotracer a specific 99mTc-radiolabeled mAb. A position-sensitive high-resolution mini-gamma camera was used as imaging capture device. Human galectin-3 positive thyroid cancer xenografts (ARO) and galectin-3 knockout tumors were used as targets in different experiments in vivo. 38 mice with tumor mass of about 1 gm were injected in the tail vein with 100 µCi of 99mTc-labeled mAb to galectin-3 (30 µg protein/in 100 µl saline solution). Tumor images were acquired at 1 hr, 3 hrs, 6 hrs, 9 hrs and 24 hrs post injection by using the mini-gamma camera.Findings
Results from different consecutive experiments show an optimal visualization of thyroid cancer xenografts between 6 and 9 hours from injection of the radiotracer. Galectin-3 negative tumors were not detected at all. At 6 hrs post-injection galectin-3 expressing tumors were correctly visualized, while the whole-body activity had essentially cleared.Conclusions
These results demonstrate the possibility to distinguish preoperatively benign from malignant thyroid nodules by using a specific galectin-3 radio-immunotargeting. In vivo imaging of thyroid cancer may allow a better selection of patients referred to surgery. The possibility to apply this method for imaging and treatment of other galectin-3 expressing tumors is also discussed. 相似文献103.
RRE: a tool for the extraction of non-coding regions surrounding annotated genes from genomic datasets 总被引:1,自引:0,他引:1
Lazzarato F Franceschinis G Botta M Cordero F Calogero RA 《Bioinformatics (Oxford, England)》2004,20(16):2848-2850
RRE allows the extraction of non-coding regions surrounding a coding sequence [i.e. gene upstream region, 5'-untranslated region (5'-UTR), introns, 3'-UTR, downstream region] from annotated genomic datasets available at NCBI. AVAILABILITY: RRE parser and web-based interface are accessible at http://www.bioinformatica.unito.it/bioinformatics/rre/rre.html 相似文献
104.
Bernardini R Johnson EO Kamilaris T Chiarenza A Cantarella G Calogero AE Lempereur L Chrousos GP Giuffrida R Gold PW 《Life sciences》2001,68(14):1657-1665
We have studied the effect of intravenous injection of interleukin-1 (dose range: from 0.25 to 4.5 microg/kg of body weight) on plasma ACTH and cortisol levels in the marmoset, a primate paradygm of peripheral glucocorticoid resistance. Blood sampling were collected and body temperature recorded 0, 15, 30, 60, 120, 180, 240 and 300 min after injection. Interleukin-1 stimulated secretion of ACTH in a dose-dependent fashion. Maximal secretion occurred 120 min after injection, and lasted up to 240 min. Plasma ACTH levels returned to baseline 300 min after interleukin-1 injection. Plasma cortisol levels were related to ACTH levels. Body temperature elevation, which occurred 10-15 min after injection was dose-dependent, and lasted 3 h. Results suggest that the pyrogenic effect of interleukin is associated, in the marmoset, with integrated activation of the hypothalamic-pituitary-adrenal axis. In light of the proneness of marmosets to hyperimmune disorders, our data are consistent with the hypothesized central biological role of IL-1, as well as the pathophysiological relevance of the neuro-endocrine-immune cross-talk during the acute phase response. 相似文献
105.
Constitutive inositol phosphate formation in cytomegalovirus-infected human fibroblasts is due to expression of the chemokine receptor homologue pUS28
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Minisini R Tulone C Lüske A Michel D Mertens T Gierschik P Moepps B 《Journal of virology》2003,77(8):4489-4501
An open reading frame (ORF), US28, with homology to mammalian chemokine receptors has been identified in the genome of human cytomegalovirus (HCMV). Its protein product, pUS28, has been shown to bind several human CC chemokines, including RANTES, MCP-1, and MIP-1 alpha, and the CX(3)C chemokine fractalkine with high affinity. Addition of CC chemokines to cells expressing pUS28 was reported to cause a pertussis toxin-sensitive increase in the concentration of cytosolic free Ca(2+). Recently, pUS28 was shown to mediate constitutive, ligand-independent, and pertussis toxin-insensitive activation of phospholipase C via G(q/11)-dependent signaling pathways in transiently transfected COS-7 cells. Since these findings are not easily reconciled with the former observations, we analyzed the role of pUS28 in mediating CC chemokine activation of pertussis toxin-sensitive G proteins in cell membranes and phospholipase C in intact cells. The transmembrane signaling functions of pUS28 were studied in HCMV-infected cells rather than in cDNA-transfected cells. Since DNA sequence analysis of ORF US28 of different laboratory and clinical strains had revealed amino acid sequence differences in the amino-terminal portion of pUS28, we compared two laboratory HCMV strains, AD169 and Toledo, and one clinical strain, TB40/E. The results showed that infection of human fibroblasts with all three HCMV strains led to a vigorous, constitutively enhanced formation of inositol phosphates which was insensitive to pertussis toxin. This effect was critically dependent on the presence of the US28 ORF in the HCMV genome but was independent of the amino acid sequence divergence of the three HCMV strains investigated. The constitutive activity of pUS28 is not explained by expression of pUS28 at high density in HCMV-infected cells. The pUS28 ligands RANTES and MCP-1 failed to stimulate binding of guanosine 5'-O-(3-[(35)S]thiotriphosphate to membranes of HCMV-infected cells and did not enhance constitutive activation of phospholipase C in intact HCMV-infected cells. These findings raise the possibility that the effects of CC chemokines and pertussis toxin on G protein-mediated transmembrane signaling previously observed in HCMV-infected cells are either independent of or not directly mediated by the protein product of ORF US28. 相似文献
106.
Muscarella Calogero Luiselli Luca Di Vittorio Massimiliano Sparacio Ignazio Amori Giovanni Dendi Daniele 《Journal of Insect Conservation》2022,26(4):619-626
Journal of Insect Conservation - Polyphylla ragusae (Coleoptera Scarabaeidae) is an endemic beetle restricted to the island of Sicily (Italy). This species is among the most threatened... 相似文献
107.
108.
Fausto Calderazzo Ulli Englertb C cilia Maichle-M ssmer Fabio Marchetti Guido Pampaloni Debora Petroni Calogero Pinzino Joachim Str hle Giovanna Tripepi 《Inorganica chimica acta》1998,270(1-2):177-188
The N,N-diethylcarbamato derivative of zirconium(IV), Zr(O2CNEt2)4 has been studied by X-ray crystallography. Crystal data: C20H40Na4O8Zr, monoclinic, space group C2/c, a = 14.057(1), b = 12.168(1), c = 16.746(2) Å, β = 108.071(4)°, Z = 4, Dc = 1.356, F(000) = 1168, T = 213 K. The compound is isotypic with the corresponding niobium(IV) derivative with a dodecahedral coordination at the zirconium atom. By reaction of NbCl4(THF)2 with Tl(hfacac), the hexafluoroacetylacetonato derivative of niobium (IV), Nb(hfacac)4, has been prepared and structurally characterized. The compound crystallizes in the orthorhombic space group Pna21 with the following cell constants: a = 10.399(4), b = 15.852(9), c = 119.073(1) Å. It is not isotypic with the corresponding zirconium(IV) derivative, Zr(hfacac)4. Crystal data: C20H4F24O8Zr, monoclinic, space group P21/n, a = 11.974(4), b = 20.451(6), c = 13.140(3) Å, β = 104.487(11)°, Z = 4, Dc = 1.960, F(000) = 1776, T = 223 K. Although in both compounds the central metal atom shows a square antiprismatic coordination, the coordination mode of the ligands is different and slight deviations from the D4(llll) and C2(llss) ideal geometries have been observed in the case of niobium and zirconium, respectively. An EPR study has been performed on the Nb(IV) derivatives as diluted solid solutions in frozen organic solvents or in the diamagnetic matrix of the corresponding zirconium(IV) compound. The EPR spectra have confirmed the presence of non-interacting paramagnets in the solid solutions and, in the case of Nb(O2CNEt2)4, the point symmetry of the paramagnetic centre has been found to be in agreement with the results of the X-ray investigation. An EPR spectrum of rhombic symmetry has been observed for the hexafluoroacetylacetonato derivative of Nb(IV) when diluted in frozen THF solution or in Zr(hfacac)4. 相似文献
109.
Relationship between size of mRNA ribosomal binding site and initiation factor function 总被引:1,自引:0,他引:1
The rate and the extent of the binding of initiator fMet-tRNA(fMet) to 30S ribosomal subunits in the presence of IF1, IF2 and GTP is either inhibited or slightly stimulated by the presence of IF3 depending on whether the initiation triplet AUG or the polynucleotide poly(AUG) is used as template. To determine the length of the template required for the transition from the AUG- to the poly(AUG)-type of behavior in the presence of IF3, the ribosomal binding of fMet-tRNA was studied in response to AUG triplets extended on either the 5'- or the 3'-side by stretches of homo-oligonucleotides of different lengths. When the binding of fMet-tRNA was studied at equilibrium it was found that IF3 no longer inhibits the amount of ternary complex formed if AUG is extended either 10 nucleotides on the 5'- or 35-40 nucleotides on the 3'-side. When the initial rate of ternary complex formation is considered, shorter extensions (4 nucleotides on the 5'-side or 20-30 nucleotides on the 3'-side) are sufficient to elicit a substantial stimulation by IF3. These results are discussed in relation to the mechanism of action of the initiation factors in the selection of the initiation region of the mRNA by ribosomes. 相似文献
110.