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61.
Allen IC Lich JD Arthur JC Jania CM Roberts RA Callaway JB Tilley SL Ting JP 《PloS one》2012,7(1):e30612
Among the 22 members of the nucleotide binding-domain, leucine rich repeat-containing (NLR) family, less than half have been functionally characterized. Of those that have been well studied, most form caspase-1 activating inflammasomes. NLRP12 is a unique NLR that has been shown to attenuate inflammatory pathways in biochemical assays and mediate the lymph node homing of activated skin dendritic cells in contact hypersensitivity responses. Since the mechanism between these two important observations remains elusive, we further evaluated the contribution of NLRP12 to organ specific adaptive immune responses by focusing on the lung, which, like skin, is exposed to both exogenous and endogenous inflammatory agents. In models of allergic airway inflammation induced by either acute ovalbumin (OVA) exposure or chronic house dust mite (HDM) antigen exposure, Nlrp12(-/-) mice displayed subtle differences in eosinophil and monocyte infiltration into the airways. However, the overall development of allergic airway disease and airway function was not significantly altered by NLRP12 deficiency. Together, the combined data suggest that NLRP12 does not play a vital role in regulating Th2 driven airway inflammation using common model systems that are physiologically relevant to human disease. Thus, the allergic airway inflammation models described here should be appropriate for subsequent studies that seek to decipher the contribution of NLRP12 in mediating the host response to agents associated with asthma exacerbation. 相似文献
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Poole TL Callaway TR Bischoff KM Loneragan GH Anderson RC Nisbet DJ 《Journal of applied microbiology》2012,113(3):659-668
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Carboxypeptidase A6 (CPA6) is a member of the M14 metallocarboxypeptidase family that is highly expressed in the adult mouse olfactory bulb and broadly expressed in embryonic brain and other tissues. A disruption in the human CPA6 gene is linked to Duane syndrome, a defect in the abducens nerve/lateral rectus muscle connection. In this study the cellular distribution, processing, and substrate specificity of human CPA6 were investigated. The 50-kDa pro-CPA6 is routed through the constitutive secretory pathway, processed by furin or a furin-like enzyme into the 37-kDa active form, and secreted into the extracellular matrix. CPA6 cleaves the C-terminal residue from a range of substrates, including small synthetic substrates, larger peptides, and proteins. CPA6 has a preference for large hydrophobic C-terminal amino acids as well as histidine. Peptides with a penultimate glycine or proline are very poorly cleaved. Several neuropeptides were found to be processed by CPA6, including Met- and Leu-enkephalin, angiotensin I, and neurotensin. Whereas CPA6 converts enkephalin and neurotensin into forms known to be inactive toward their receptors, CPA6 converts inactive angiotensin I into the biologically active angiotensin II. Taken together, these data suggest a role for CPA6 in the regulation of neuropeptides in the extracellular environment within the olfactory bulb and other parts of the brain. 相似文献
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JC. Brisset F. Gazeau C. Corot N. Nighoghossian Y. Berthezène E. Canet-Soulas M. Wiart 《IRBM》2018,39(2):93-102
Background
Inflammation is a share process in atherosclerosis and stroke and is thought to be a key player in the evolution of these diseases. Ten years ago, inflammation imaging with magnetic resonance imaging (MRI) was considered very promising for both pre-clinical and clinical studies of atherosclerosis and stroke.Contribution
We report here contributions to the field of inflammation imaging with USPIO-enhanced MRI. The goal was to investigate the life cycle of USPIOs in the body, and how the MRI signal has been impacted during their bio-interactions and bioprocessing. Those mechanisms were applied to pre-clinical longitudinal studies of inflammation in atherosclerosis and at the acute stage of ischemic stroke thus allowing the monitoring of treatment effects.Conclusion
This review presents the contribution of the collaborative research project under the “TecSan” grant from the French Research Agency (ANR) as well as pre-clinical and clinical perspectives of USPIO's inflammation MRI in atherosclerosis and stroke. 相似文献68.
Animal, fungal, and bacterial consumers can have dramatic effects on the structure of plant communities, often by consuming
dominant competitors and indirectly increasing the abundance of inferior competitors. We investigated the role of a consumer
plant, the parasite Cuscuta salina, on plant zonation in a western salt marsh. Cuscuta had a strong host species preference in experiments, disproportionally infecting Salicornia virginica, the dominant competitor in most of the marsh. In plots with Cuscuta, which infected 18% of our study area over a 3-year period, Salicornia cover decreased and the cover of Arthrocnemum increased substantially in comparison to plots without Cuscuta. Deep in the Salicornia zone, the cover of Arthrocnemum in Cuscuta-infected plots increased by 558% in 1 year relative to uninfected plots. At the ecotone, the cover of Arthrocnemum in Cuscuta-infected plots increased by only 41% during the same time interval. These data suggest that the relative benefit of a consumer
to a less-preferred, subordinate competitor may be strongest where competition is the most asymmetrical as predicted by recent
theoretical models. By weakening the competitive dominant, which in the absence of the parasite can create virtual monocultures,
Cuscuta enhanced community diversity and altered the ecotone between Salicornia and Arthrocnemum. Cuscuta patches were highly dynamic at the ecotone between Salicornia and Arthrocnemum, and thus the changes we measured in our sample plots were likely to be representative of large portions of the marsh. Our
findings emphasize the importance of trophic interactions in salt marsh structure and zonation.
Received: 23 April 1997 / Accepted: 10 October 1997 相似文献
69.
The Ability of “Low G + C Gram-Positive” Ruminal Bacteria to Resist Monensin and Counteract Potassium Depletion 总被引:1,自引:0,他引:1
Gram-negative ruminal bacteria with an outer membrane are generally more resistant to the feed additive, monensin, than Gram-positive
species, but some bacteria can adapt and increase their resistance. 16S rRNA sequencing indicates that a variety of ruminal
bacteria are found in the “low G + C Gram-positive group,” but some of these bacteria are monensin resistant and were previously
described as Gram-negative species (e.g., Selenomonas ruminantium and Megasphaera elsdenii). The activity of monensin can be assayed by its ability to cause potassium loss, and results indicated that the amount of
monensin needed to catalyze half maximal potassium depletion (Kd) from low G + C gram-positive ruminal bacteria varied by as much as 130-fold. The Kd values for Butyrivibrio fibrisolvens 49, Streptococcus bovis JB1, Clostridium aminophilum F, S. ruminantium HD4, and M. elsdenii B159 were 10, 65, 100, 1020, and 1330 nM monensin, respectively. B. fibrisolvens was very sensitive to monensin, and it did not adapt. S. bovis and C. aminophilum cultures that were transferred repeatedly with sub-lethal doses of monensin had higher Kd values than unadapted cultures, but the Kd was always less than 800 nM. S. ruminantium and M. elsdenii cells were highly resistant (Kd > 1000 nM), and this resistance could be explained by the ability of these low G + C Gram-positive bacteria to synthesize
outer membranes.
Received: 14 May 1999 / Accepted: 24 June 1999 相似文献
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