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31.
Snyder VL Turner M Li PK El-Sharkawy A Dunphy G Ely DL 《The Journal of steroid biochemistry and molecular biology》2000,73(5):251-256
The objective of this study was to examine the response of tissue steroid sulfatase (STS) levels in hypertensive rat strains, when blood pressure (BP) was lowered by different techniques at an early age. A 4×3 factoral design was used, in which males (n=6–8) from four rat strains (WKY, SHR, SHR/a, SHR/y) at 4 weeks of age, were randomly assigned to one of three treatment groups: a hydralazine group, a castration group and a control group. BP was measured by the tail cuff technique and verified by tail catheter at the end of the experiment. BP was significantly reduced by both treatments in the hypertensive strains (SHR, SHR/a, SHR/y) compared to respective control groups. At 15–17 weeks of age, animals were euthanized and heart, kidney, adrenal glands and liver were assayed for STS levels. The major trend in tissue STS was that castration significantly lowered: adrenal, heart and liver STS in specific strains. In conclusion, castration and hydralazine significantly lowered the BP in the hypertensive rat strains, but only castration consistently lowered STS levels across strains implicating testosterone as a regulator of tissue STS. 相似文献
32.
Fungi and vascular plant interactions are necessary components of natural community establishment, productivity and degradation. While many fungal species serve as decomposers of organic matter, others have evolved mutualistic or parasitic relationships with vascular plants. This research focused on characterizing associations among macrofungi, vascular plant communities and soils. Ha Ha Tonka State Park is in central Missouri and has a varying landscape with numerous natural community types that provide diverse habitats and microhabitats that are ideally suited to the investigation of fungal, floral and soil associations. Five communities sampled within the park included glades, open woodlands, flatwoods, closed-canopy forests and karst sinks. Permanent 0.01 ha. plots were surveyed in the 2006 and 2007 growing seasons. Surveys of plots and entire communities yielded 249 fungal taxa and approximately 265 floral taxa. Soils were analyzed to help define specific edaphic components of each community and used to associate soil attributes with plant and fungal communities. Forest communities contained the most ectomycorrhizal (ECM) fungi species. Karst sinks and glades had higher soil pH and phosphorus and fewer ectomycorrhizal fungi. Statistical analyses included non-metric multidimensional scaling, multiresponse permutation procedure and indicator species analysis. Indicator species were identified for flatwood, forest and karst communities, but results were inconclusive for glades and open woodlands. 相似文献
33.
Unprotected streams within the agricultural Midwest region of the United States are subject to sedimentation, nutrification, and agricultural chemicals. Grass riparian filter strips (GRFSs) have been implemented as a best management practice to minimize sedimentation and associated materials that are harmful to aquatic ecosystems; however, few studies have examined the benthic community response to GRFS installation. This study introduces a least-desired index (LDI) multimetric approach of evaluating benthic communities in response to GRFS installation. LDI was determined in a reciprocal fashion to that of a benthic macroinvertebrate index of biotic integrity (B-IBI). When reference conditions are not available for the use of B-IBI, anti-reference sites, representing least-desired conditions, can be used in constructing an LDI. A B-IBI and LDI were constructed in the Claypan Till Plains Subsection of Missouri and comparatively used to evaluate two test sites where tall fescue GRFS were installed. Five metrics were used to develop the B-IBI and six for the LDI. The LDI tended to be more conservative at evaluation in comparison to the B-IBI. Paired t-tests showed that LDI and B-IBI were significantly different at scoring test sites. The LDI assessed both test sites as showing no response to GRFS installation, whereas the B-IBI suggested moderate improvement. The LDI was considered to be a better index for evaluation because the streams used to develop the B-IBI were not suitable reference sites. An argument for the use of chironomid based metrics in low gradient agricultural streams is presented. 相似文献
34.
Ely KH Ahmed M Kohlmeier JE Roberts AD Wittmer ST Blackman MA Woodland DL 《Journal of immunology (Baltimore, Md. : 1950)》2007,179(6):3535-3542
Increasing age is associated with the development of CD8+ T cell clonal expansions (TCE) that can dominate the peripheral T cell repertoire and interfere with immune responses to infection and vaccination. Some TCE are driven by chronic infections, consistent with dysregulated outgrowth of T cell clones in response to persistent antigenic stimulation. However, a second class of TCE develops with age in the absence of chronic infections and is poorly understood in terms of origin or Ag dependence. In this study, we present evidence that Ag-specific TCE develop at high frequencies from conventional memory CD8+ T cell pools elicited by nonpersistent influenza and parainfluenza virus infections. Putative TCE occurred in both the central- and effector-memory CD8+ T cell populations and did not require Ag for their maintenance. In addition, they were similar to normal memory T cells in terms of phenotype and function, suggesting that they develop stochastically from the memory T cell pool. These data suggest that memory T cell pools become progressively dysregulated over time and this may have a significant impact on immune responsiveness in the aged. 相似文献
35.
Ely JT 《Experimental biology and medicine (Maywood, N.J.)》2007,232(7):847-851
In this Comment, the ultimate intent is to increase survival of the anticipated global flu pandemic. The apparent failure of "medicine" to provide a completely understood and logically based biochemical prevention and treatment for all influenzas (and many other viral diseases) may be an unavoidable result of the evolving complexity of the H5N1 virus. However, clinical experience cited in all accounts, including the 2003 to 2006 period, suggest that: (i) ascorbic acid is not being administered to humans infected or at risk for influenza, and (ii) ascorbic acid is (mistakenly) believed to be a vitamin ("vitamin C"). Proper use of ascorbic acid as described here could provide effective containment for the flu pandemic. 相似文献
36.
37.
Background
Moral sensitivity refers to the interpretive awareness of moral conflict and can be justice or care oriented. Justice ethics is associated primarily with human rights and the application of moral rules, whereas care ethics is related to human needs and a situational approach involving social emotions. Among the core brain regions involved in moral issue processing are: medial prefrontal cortex, anterior (ACC) and posterior (PCC) cingulate cortex, posterior superior temporal sulcus (pSTS), insula and amygdala. This study sought to inform the long standing debate of whether care and justice moral ethics represent one or two different forms of cognition.Methodology/Principal Findings
Model-free and model-based connectivity analysis were used to identify functional neural networks underlying care and justice ethics for a moral sensitivity task. In addition to modest differences in patterns of associated neural activity, distinct modes of functional and effective connectivity were observed for moral sensitivity for care and justice issues that were modulated by individual variation in moral ability.Conclusions/Significance
These results support a neurobiological differentiation between care and justice ethics and suggest that human moral behavior reflects the outcome of integrating opposing rule-based, self-other perspectives, and emotional responses. 相似文献38.
Across bacterial phyla, distantly-related genomes with similar genomic GC content have similar patterns of amino acid usage 总被引:1,自引:0,他引:1
The GC content of bacterial genomes ranges from 16% to 75% and wide ranges of genomic GC content are observed within many bacterial phyla, including both gram negative and gram positive phyla. Thus, divergent genomic GC content has evolved repeatedly in widely separated bacterial taxa. Since genomic GC content influences codon usage, we examined codon usage patterns and predicted protein amino acid content as a function of genomic GC content within eight different phyla or classes of bacteria. We found that similar patterns of codon usage and protein amino acid content have evolved independently in all eight groups of bacteria. For example, in each group, use of amino acids encoded by GC-rich codons increased by approximately 1% for each 10% increase in genomic GC content, while the use of amino acids encoded by AT-rich codons decreased by a similar amount. This consistency within every phylum and class studied led us to conclude that GC content appears to be the primary determinant of the codon and amino acid usage patterns observed in bacterial genomes. These results also indicate that selection for translational efficiency of highly expressed genes is constrained by the genomic parameters associated with the GC content of the host genome. 相似文献
39.
40.
Alvarado Bremer JR Viñas J Mejuto J Ely B Pla C 《Molecular phylogenetics and evolution》2005,36(1):169-187
Comparative phylogeography has revealed remarkable patterns of concordance in the maternal phylogenies of many species. The phylogeography and historical demography of the mitochondrial control region I for 607 Atlantic bluefin tuna (Thunnus thynnus) and 275 swordfish (Xiphias gladius) were analyzed to clarify the complex phylogenetic signals in the North Atlantic-Mediterranean region where they are sympatric. Atlantic bluefin tuna mtDNA is polyphyletic, and includes rare sequences sister to Pacific bluefin tuna (Thunnus orientalis) and introgressed albacore (Thunnus alalunga) sequences. There is no geographic partitioning between Atlantic and Mediterranean samples of Atlantic bluefin tuna (Phi(ST)=0.002). In contrast, Atlantic and Mediterranean swordfish are differentiated (Phi(ST)=0.091) due to the combined effects of vicariance, secondary contact, and dissimilar regional demographic histories. Mediterranean swordfish has substantially less variation, and a more recent history (tau=2.42) than that of Atlantic swordfish (tau=7.02). In spite of the discordant phylogenetic and phylogeographic signals, the demographic history of Atlantic swordfish and Atlantic bluefin tuna (tau=7.51) suggests concordance in the timeline of population expansion. Possible scenarios of cladogenesis, expansion, and contraction, influenced by glacial cycles during the Pleistocene, are formulated. 相似文献