全文获取类型
收费全文 | 8262篇 |
免费 | 657篇 |
国内免费 | 598篇 |
专业分类
9517篇 |
出版年
2024年 | 16篇 |
2023年 | 104篇 |
2022年 | 266篇 |
2021年 | 435篇 |
2020年 | 306篇 |
2019年 | 347篇 |
2018年 | 371篇 |
2017年 | 251篇 |
2016年 | 358篇 |
2015年 | 503篇 |
2014年 | 571篇 |
2013年 | 599篇 |
2012年 | 759篇 |
2011年 | 634篇 |
2010年 | 384篇 |
2009年 | 369篇 |
2008年 | 408篇 |
2007年 | 378篇 |
2006年 | 347篇 |
2005年 | 277篇 |
2004年 | 237篇 |
2003年 | 202篇 |
2002年 | 175篇 |
2001年 | 143篇 |
2000年 | 114篇 |
1999年 | 133篇 |
1998年 | 79篇 |
1997年 | 89篇 |
1996年 | 80篇 |
1995年 | 74篇 |
1994年 | 85篇 |
1993年 | 60篇 |
1992年 | 73篇 |
1991年 | 69篇 |
1990年 | 60篇 |
1989年 | 33篇 |
1988年 | 36篇 |
1987年 | 25篇 |
1986年 | 19篇 |
1985年 | 23篇 |
1984年 | 9篇 |
1983年 | 11篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1978年 | 1篇 |
排序方式: 共有9517条查询结果,搜索用时 0 毫秒
11.
报道了缢蛏碱性磷酸酶(简称ALP)经不同浓度盐酸胍处理时酶的分子构象所发生的变化以及酶变化和失活的动力学过程。在胍中酶荧光发射峰强度下降,紫外差光谱在246nm和285nm处出现2个负峰,CD谱中酶的α螺旋度下降,且随浓度增大,变化程度也加大。动力学研究表明,酶在0.5mol/L、1.0mol/L、2.0mol/L3.0mol/L、4.0mol/L盐酸胍中的变性速度常数分别为3.21×10~(-4)s~(-1)、6.38×10~(-4)s~(-1)、2.17×10~(-3)s~(-1)、2.33×10~(-3)s~9-1)、5.17×10~(-3)s~(-1);而酶在相应盐酸胍中的失活速度常数分别为2.33×10~(-4)s~(-1)、3.57×10~(-4)s~(-1)、5.86×10~(-4)s~(-1)、1.14×10~(-3)s~(-1)、3.45×10~(-3)s~(-1);表现为失活与构象伸展变化基本平行。 相似文献
12.
The localization of neuropeptide Y (NPY) and atrial natriuretic peptide (ANP) in the endothelial cells of human umbilical blood vessels was studied using the pre-embedding peroxidase-antiperoxidase (PAP) technique for electron microscopy and avidin-biotin-complex (ABC) immunostaining for endothelial cells cultured from umbilical vein. Subpopulations of NPY- and ANP-immunoreactive endothelial cells were present in term umbilical vein and artery. The umbilical vein contained more positive cells than the artery. The percentage of NPY- and ANP-immunoreactive umbilical vein cells in culture was 32% and 44%, respectively, out of a total of 3013 cells examined. The possibility that these potent vasoactive substances located in the endothelial cells of the non-innervated umbilical vessels are involved in the local regulation of blood flow is discussed. 相似文献
13.
Hydrolysis of phosphatidylcholine couples Ras to activation of Raf protein kinase during mitogenic signal transduction. 总被引:12,自引:6,他引:6 下载免费PDF全文
H Cai P Erhardt J Troppmair M T Diaz-Meco G Sithanandam U R Rapp J Moscat G M Cooper 《Molecular and cellular biology》1993,13(12):7645-7651
We have investigated the relationship between hydrolysis of phosphatidylcholine (PC) and activation of the Raf-1 protein kinase in Ras-mediated transduction of mitogenic signals. As previously reported, cotransfection of a PC-specific phospholipase C (PC-PLC) expression plasmid bypassed the block to cell proliferation resulting from expression of the dominant inhibitory mutant Ras N-17. In contrast, PC-PLC failed to bypass the inhibitory effect of dominant negative Raf mutants, suggesting that PC-PLC functions downstream of Ras but upstream of Raf. Consistent with this hypothesis, treatment of quiescent cells with exogenous PC-PLC induced Raf activation, even when normal Ras function was blocked by Ras N-17 expression. Further, activation of Raf in response to mitogenic growth factors was blocked by inhibition of endogenous PC-PLC. Taken together, these results indicate that hydrolysis of PC mediates Raf activation in response to mitogenic growth factors. 相似文献
14.
汉族ABO血型的皮纹特征分析 总被引:2,自引:1,他引:1
作者对芜湖地区382例(男220人;女162人)汉族ABO血型的皮纹特征进行了分析,其中O型130人,A型113人,B型101人,AB型38人。分析比较了指纹类型、指纹组合格局、指嵴纹计数、掌嵴纹计数、atd角,掌部真实花纹,掌褶纹和拇趾球纹等项参数,结果表明,ABO各血型的皮纹参数间有若干统计学差异。 相似文献
15.
16.
Six cDNAs representing unique cold-induced sequences have been cloned from the hardy citrus relative Poncirus trifoliata. Among these, pBCORc115 and pBCORc119 were found to belong to the same gene family. Sequencing data indicated that pBCORc115 and pBCORc119 each contained an open reading frame, coding for a 19.8 kDa protein (COR19) and a smaller 11.4 kDa protein (COR11) respectively. Inspection of the deduced amino acid sequences revealed three large repeats in COR19, but only one was present in the COR11. Two elements: a Q-clustered tract and a K-rich motif were identified in each repeat. The K-rich motifs were similar to those of cotton D-11 and Group 2 LEA proteins. A Serine-cluster, a common feature in many Group 2 LEA-like proteins, was also found in these proteins, but it was in an unusual position at the carboxy-terminus. A bipartite motif of basic residues, similar to known nuclear targeting sequences, was also present in COR19 and COR11, suggesting that members of this protein family may have a nuclear targeting function. The expression of COR19 mRNA in response to cold acclimation, drought, flooding, and salinization was examined. COR19 expression in leaf tissue was induced in response to cold acclimation, but repressed during drought and flooding stress. 相似文献
17.
18.
Characterization of the IS895 family of insertion sequences from the cyanobacterium Anabaena sp. strain PCC 7120. 下载免费PDF全文
J Alam J M Vrba Y Cai J A Martin L J Weislo S E Curtis 《Journal of bacteriology》1991,173(18):5778-5783
A family of repetitive elements from the cyanobacterium Anabaena sp. strain PCC 7120 was identified through the proximity of one element to the psbAI gene. Four members of this seven-member family were isolated and shown to have structures characteristic of bacterial insertion sequences. Each element is approximately 1,200 bp in length, is delimited by a 30-bp inverted repeat, and contains two open reading frames in tandem on the same DNA strand. The four copies differ from each other by small insertions or deletions, some of which alter the open reading frames. By using a system designed to trap insertion elements, one of the elements, denoted IS895, was shown to be mobile. The target site was not duplicated upon insertion of the element. Two other filamentous cyanobacterial strains were also found to contain sequences homologous to IS895. 相似文献
19.
20.