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251.
252.
Andrea Moglia Sergio Lanteri Cinzia Comino Lionel Hill Daniel Knevitt Cecilia Cagliero Patrizia Rubiolo Stephen Bornemann Cathie Martin 《Plant physiology》2014,166(4):1777-1787
Tomato (Solanum lycopersicum), like other Solanaceous species, accumulates high levels of antioxidant caffeoylquinic acids, which are strong bioactive molecules and protect plants against biotic and abiotic stresses. Among these compounds, the monocaffeoylquinic acids (e.g. chlorogenic acid [CGA]) and the dicaffeoylquinic acids (diCQAs) have been found to possess marked antioxidative properties. Thus, they are of therapeutic interest both as phytonutrients in foods and as pharmaceuticals. Strategies to increase diCQA content in plants have been hampered by the modest understanding of their biosynthesis and whether the same pathway exists in different plant species. Incubation of CGA with crude extracts of tomato fruits led to the formation of two new products, which were identified by liquid chromatography-mass spectrometry as diCQAs. This chlorogenate:chlorogenate transferase activity was partially purified from ripe fruit. The final protein fraction resulted in 388-fold enrichment of activity and was subjected to trypsin digestion and mass spectrometric sequencing: a hydroxycinnamoyl-Coenzyme A:quinate hydroxycinnamoyl transferase (HQT) was selected as a candidate protein. Assay of recombinant HQT protein expressed in Escherichia coli confirmed its ability to synthesize diCQAs in vitro. This second activity (chlorogenate:chlorogenate transferase) of HQT had a low pH optimum and a high Km for its substrate, CGA. High concentrations of CGA and relatively low pH occur in the vacuoles of plant cells. Transient assays demonstrated that tomato HQT localizes to the vacuole as well as to the cytoplasm of plant cells, supporting the idea that in this species, the enzyme catalyzes different reactions in two subcellular compartments.The importance of plant-based foods in preventing or reducing the risk of chronic disease has been widely demonstrated (Martin et al., 2011, 2013). In addition to vitamins, a large number of other nutrients in plant-based foods promote health and reduce the risk of chronic diseases; these are often referred to as phytonutrients. The presence of phytonutrients in fruit and vegetables is of significant nutritional and therapeutic importance, as many have been found to possess strong antioxidant activity (Rice-Evans et al., 1997). Phenolics are the most widespread dietary antioxidants and caffeoylquinic acids, such as chlorogenic acid (CGA), dicaffeoylquinic acids (diCQAs), and tricaffeoylquinic acids (triCQAs), play important roles in promoting health (Clifford, 1999; Niggeweg et al., 2004). CGA limits low density lipid oxidation (Meyer et al., 1998), diCQAs possess antihepatotoxic activity (Choi et al., 2005), and triCQAs reduce the blood Glc levels of diabetic rats (Islam, 2006). diCQA derivatives have been shown to protect humans from various kinds of diseases; diCQAs suppress melanogenesis effectively (Kaul and Khanduja, 1998), show anti-inflammatory activity in vitro (Peluso et al., 1995), and exhibit a selective inhibition of HIV replication (McDougall et al., 1998). The physiological effects of caffeoylquinic acid derivatives with multiple caffeoyl groups are generally greater than those of monocaffeoylquinic acids, perhaps because the antioxidant activity is largely determined by the number of hydroxyl groups present on the aromatic rings (Wang et al., 2003; Islam, 2006). Furthermore, both diCQAs and triCQAs may function as inhibitors of the activity of HIV integrase, which catalyzes the insertion of viral DNA into the genome of host cells (McDougall et al., 1998; Slanina et al., 2001; Gu et al., 2007).CGA is the major soluble phenolic in Solanaceous crops (Clifford, 1999) and the major antioxidant in the average U.S. diet (Luo et al., 2008), while different isomers of diCQAs have been identified in many crops such as coffee (Coffea canephora), globe artichoke (Cynara cardunculus), tomato (Solanum lycopersicum), lettuce (Lactuca sativa), and sweet potato (Ipomoea batatas; Clifford, 1999; Islam, 2006; Moco et al., 2006, 2007; Moglia et al., 2008). In tomato, CGA accounts for 75% and 35% of the total phenolics in mature green and ripe fruit, respectively, amounting to 2 to 40 mg 100 g–1 dry weight (DW), although levels decline after ripening and during postharvest storage (Slimestad and Verheul, 2009). diCQAs and triCQAs also accumulate in tomato fruit (diCQAs, approximately 2 mg 100 g–1
DW; and triCQAs, 1–2 mg 100 g–1
DW; Chanforan et al., 2012).Three pathways (Villegas and Kojima, 1986; Hoffmann et al., 2003; Niggeweg et al., 2004) have been proposed for the synthesis of CGA: (1) the direct pathway involving caffeoyl-CoA transesterification with quinic acid by hydroxycinnamoyl-Coenzyme A:quinate hydroxycinnamoyl transferase (HQT; Niggeweg et al., 2004; Comino et al., 2009; Menin et al., 2010; Sonnante et al., 2010); (2) the route by which p-coumaroyl-CoA is first transesterified with quinic acid via hydroxycinnamoyl-Coenzyme A transferase (HCT) acyltransferase (Hoffmann et al., 2003; Comino et al., 2007), followed by the hydroxylation of p-coumaroyl quinate to 5-caffeoylquinic acid, catalyzed by C3′H (p-coumaroyl-3-hydroxylase; Schoch et al., 2001; Mahesh et al., 2007; Moglia et al., 2009); and (3) the use of caffeoyl-glucoside as the acyl-donor (Villegas and Kojima, 1986). In tomato, the synthesis of CGA involves transesterification of caffeoyl-CoA with quinic acid by HQT (Niggeweg et al., 2004).To date, it is not clear whether diCQAs are derived directly from the monocaffeoylquinic acids (such as CGA) through a second acyltransferase reaction involving an acyl-CoA or not, although their structural similarity provides good a priori evidence supporting this hypothesis. Recently the in vitro synthesis of 3,5-diCQA from CGA and CoA by HCT from coffee has been reported (Lallemand et al., 2012). By contrast, in sweet potato, an enzyme that catalyzes the transfer of the caffeoyl moiety of CGA to another molecule of CGA, leading to the synthesis of isochlorogenate (3,5-di-O-caffeoylquinate), has been described, but the corresponding gene has not been identified (Villegas and Kojima, 1986).We report a chlorogenate:chlorogenate transferase (CCT) activity leading to the synthesis of diCQAs in tomato fruits and describe how alternative catalysis, by a single enzyme, leads to the production of both CGA and diCQA in different cellular compartments. 相似文献
253.
Vertebrate cranial placodes are crucial contributors to the vertebrate cranial sensory apparatus. Their evolutionary origin has attracted much attention from evolutionary and developmental biologists, yielding speculation and hypotheses concerning their putative homologues in other lineages and the developmental and genetic innovations that might have underlain their origin and diversification. In this article we first briefly review our current understanding of placode development and the cell types and structures they form. We next summarise previous hypotheses of placode evolution, discussing their strengths and caveats, before considering the evolutionary history of the various cell types that develop from placodes. In an accompanying review, we also further consider the evolution of ectodermal patterning. Drawing on data from vertebrates, tunicates, amphioxus, other bilaterians and cnidarians, we build these strands into a scenario of placode evolutionary history and of the genes, cells and developmental processes that underlie placode evolution and development. 相似文献
254.
Fazeelat Karamat Alexandre Olry Ryosuke Munakata Takao Koeduka Akifumi Sugiyama Cedric Paris Alain Hehn Frédéric Bourgaud Kazufumi Yazaki 《The Plant journal : for cell and molecular biology》2014,77(4):627-638
Furanocoumarins constitute a sub‐family of coumarin compounds with important defense properties against pathogens and insects, as well as allelopathic functions in plants. Furanocoumarins are divided into two sub‐groups according to the alignment of the furan ring with the lactone structure: linear psoralen and angular angelicin derivatives. Determination of furanocoumarin type is based on the prenylation position of the common precursor of all furanocoumarins, umbelliferone, at C6 or C8, which gives rise to the psoralen or angelicin derivatives, respectively. Here, we identified a membrane‐bound prenyltransferase PcPT from parsley (Petroselinum crispum), and characterized the properties of the gene product. PcPT expression in various parsley tissues is increased by UV irradiation, with a concomitant increase in furanocoumarin production. This enzyme has strict substrate specificity towards umbelliferone and dimethylallyl diphosphate, and a strong preference for the C6 position of the prenylated product (demethylsuberosin), leading to linear furanocoumarins. The C8‐prenylated derivative (osthenol) is also formed, but to a much lesser extent. The PcPT protein is targeted to the plastids in planta. Introduction of this PcPT into the coumarin‐producing plant Ruta graveolens showed increased consumption of endogenous umbelliferone. Expression of PcPT and a 4–coumaroyl CoA 2'–hydroxylase gene in Nicotiana benthamiana, which does not produce furanocoumarins, resulted in formation of demethylsuberosin, indicating that furanocoumarin production may be reconstructed by a metabolic engineering approach. The results demonstrate that a single prenyltransferase, such as PcPT, opens the pathway to linear furanocoumarins in parsley, but may also catalyze the synthesis of osthenol, the first intermediate committed to the angular furanocoumarin pathway, in other plants. 相似文献
255.
256.
257.
Charlotte T. Lee Cedric O. Puleston Shripad Tuljapurkar 《Theoretical population biology》2009,76(3):179-188
The population dynamics of preindustrial societies depend intimately on their surroundings, and food is a primary means through which environment influences population size and individual well-being. Food production requires labor; thus, dependence of survival and fertility on food involves dependence of a population’s future on its current state. We use a perturbation approach to analyze the effects of random environmental variation on this nonlinear, age-structured system. We show that in expanding populations, direct environmental effects dominate induced population fluctuations, so environmental variability has little effect on mean hunger levels, although it does decrease population growth. The growth rate determines the time until population is limited by space. This limitation introduces a tradeoff between population density and well-being, so population effects become more important than the direct effects of the environment: environmental fluctuation increases mortality, releasing density dependence and raising average well-being for survivors. We discuss the social implications of these findings for the long-term fate of populations as they transition from expansion into limitation, given that conditions leading to high well-being during growth depress well-being during limitation. 相似文献
258.
Ana Arbeloa James Garnett James Lillington Richard R. Bulgin Cedric N. Berger Susan M. Lea Steve Matthews Gad Frankel 《Cellular microbiology》2010,12(5):654-664
We investigated how the type III secretion system WxxxE effectors EspM2 of enterohaemorrhagic Escherichia coli, which triggers stress fibre formation, and SifA of Salmonella enterica serovar Typhimurium, which is involved in intracellular survival, modulate Rho GTPases. We identified a direct interaction between EspM2 or SifA and nucleotide‐free RhoA. Nuclear Magnetic Resonance Spectroscopy revealed that EspM2 has a similar fold to SifA and the guanine nucleotide exchange factor (GEF) effector SopE. EspM2 induced nucleotide exchange in RhoA but not in Rac1 or H‐Ras, while SifA induced nucleotide exchange in none of them. Mutating W70 of the WxxxE motif or L118 and I127 residues, which surround the catalytic loop, affected the stability of EspM2. Substitution of Q124, located within the catalytic loop of EspM2, with alanine, greatly attenuated the RhoA GEF activity in vitro and the ability of EspM2 to induce stress fibres upon ectopic expression. These results suggest that binding of SifA to RhoA does not trigger nucleotide exchange while EspM2 is a unique Rho GTPase GEF. 相似文献
259.
Long noncoding RNAs with enhancer-like function in human cells 总被引:5,自引:0,他引:5
Ørom UA Derrien T Beringer M Gumireddy K Gardini A Bussotti G Lai F Zytnicki M Notredame C Huang Q Guigo R Shiekhattar R 《Cell》2010,143(1):46-58
260.
To stabilize neutrophil polarity, PIP3 and Cdc42 augment RhoA activity at the back as well as signals at the front 总被引:1,自引:0,他引:1
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Van Keymeulen A Wong K Knight ZA Govaerts C Hahn KM Shokat KM Bourne HR 《The Journal of cell biology》2006,174(3):437-445
Chemoattractants like f-Met-Leu-Phe (fMLP) induce neutrophils to polarize by triggering divergent signals that promote the formation of protrusive filamentous actin (F-actin; frontness) and RhoA-dependent actomyosin contraction (backness). Frontness locally inhibits backness and vice versa. In neutrophil-like HL60 cells, blocking phosphatidylinositol-3,4,5-tris-phosphate (PIP3) accumulation with selective inhibitors of PIP3 synthesis completely prevents fMLP from activating a PIP3-dependent kinase and Cdc42 but not from stimulating F-actin accumulation. PIP3-deficient cells show reduced fMLP-dependent Rac activity and unstable pseudopods, which is consistent with the established role of PIP3 as a mediator of positive feedback pathways that augment Rac activation at the front. Surprisingly, such cells also show reduced RhoA activation and RhoA-dependent contraction at the trailing edge, leading to the formation of multiple lateral pseudopods. Cdc42 mediates PIP3's positive effect on RhoA activity. Thus, PIP3 and Cdc42 maintain stable polarity with a single front and a single back not only by strengthening pseudopods but also, at longer range, by promoting RhoA-dependent actomyosin contraction at the trailing edge. 相似文献