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111.
The C‐terminal segment (residues 218–289) of the HET‐s protein of the filamentous fungus Podosporina anserina is a prion‐forming domain. The structural model of the HET‐s(218–289) amyloid fibril based on solid‐state nuclear magnetic resonance (NMR) restraints shows a β solenoid topology which is comprised of a β‐sheet core and interconnecting loops. For the single‐point mutants Phe286Ala and Trp287Ala, slower aggregation rates in vitro and loss of prionic infectivity have been reported recently. Here we have used molecular dynamics to compare the flexibility of the mutants and wild type. The simulations, initiated from a trimeric aggregate extracted from the NMR structural model, show structural stability on a 100‐ns time scale for wild type and mutants. Analysis of the fluctuations along the simulations reveals that the mutants are less flexible than the wild type in the C‐terminal segment at only one of the two external monomers. Analysis of interaction energy and buried accessible surface indicates that residue Phe286 in particular is stabilized in the Trp287Ala mutant. The simulation results provide an atomistic explanation of the suggestion (based on indirect experimental evidence) that flexibility at the protofibril end(s) is required for fibril elongation. Moreover, they provide further evidence that the growth of the HET‐s amyloid fibril is directional. Proteins 2014; 82:399–404. © 2013 Wiley Periodicals, Inc. 相似文献
112.
Acid loss in the human duodenum 总被引:2,自引:0,他引:2
113.
A micro pCO2 electrode 总被引:1,自引:0,他引:1
By utilizing a previously developed micro pH glass electrode it has been possible to make a micro pCO2 electrode with a tip diameter of about 10 μm. This was accomplished by placing the micro pH electrode in a conical tube containing a weak NaHCO3 solution. The tip of the conical tube was closed with Teflon® oil wax mixture. This closure prevented the flow of solution, but allowed CO2 to pass into the NaHCO3 solution thus altering the pH of this solution. Changes in pH were seen and measured by the micro pH electrode and could be related to the pCO2 of gas or solution in which the total electrode system was placed. This electrode, principally because of its small size, has many possible applications in biological research. 相似文献
114.
Simon Messner Matthias Altmeyer Hongtao Zhao Andrea Pozivil Bernd Roschitzki Peter Gehrig Dorothea Rutishauser Danzhi Huang Amedeo Caflisch Michael O. Hottiger 《Nucleic acids research》2010,38(19):6350-6362
The chromatin-associated enzyme PARP1 has previously been suggested to ADP-ribosylate histones, but the specific ADP-ribose acceptor sites have remained enigmatic. Here, we show that PARP1 covalently ADP-ribosylates the amino-terminal histone tails of all core histones. Using biochemical tools and novel electron transfer dissociation mass spectrometric protocols, we identify for the first time K13 of H2A, K30 of H2B, K27 and K37 of H3, as well as K16 of H4 as ADP-ribose acceptor sites. Multiple explicit water molecular dynamics simulations of the H4 tail peptide into the catalytic cleft of PARP1 indicate that two stable intermolecular salt bridges hold the peptide in an orientation that allows K16 ADP-ribosylation. Consistent with a functional cross-talk between ADP-ribosylation and other histone tail modifications, acetylation of H4K16 inhibits ADP-ribosylation by PARP1. Taken together, our computational and experimental results provide strong evidence that PARP1 modifies important regulatory lysines of the core histone tails. 相似文献
115.