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S Larroudé N Massei P Reyes‐Marchant C Delattre JF Humbert 《Global Change Biology》2013,19(5):1620-1631
The impact of climate change and of other anthropogenic pressures on the structure and composition of phytoplankton communities of large European rivers remains poorly documented. Here we report the findings of a study of the changes in the phytoplankton community of the middle segment of the river Loire over the past 24 years. An attempt is made to distinguish between the impact of changes acting at the local scale and that of those acting more globally. A dramatic reduction in phytoplankton abundance was observed, particularly in the mid ‐1990s; this was concomitant with an increase in the relative proportion of cyanobacteria. At the same time, the phytoplankton community displayed increasing richness and diversity, and little change in its size structure. All these changes seem to be related to local changes, in particular to the reduction in phosphorus concentrations, as well as to changes in climate, throughout modifications in the river discharge and water temperature. Interestingly, herbicide contamination also appeared to be of particular importance in explaining the unexpected increase in the proportion of cyanobacteria in the phytoplankton community after the 1990s. These findings suggest that combinations of numerous anthropogenic pressures acting at different spatial and temporal scales have led to a mix of predictable and unpredictable changes occurring in the phytoplankton community of the river Loire, with probable consequences for the trophic networks in this river. 相似文献
63.
Victoria?PetriEmail author G?Thomas?Hayman Marek?Tutaj Jennifer?R?Smith Stanley?JF?Laulederkind Shur-Jen?Wang Rajni?Nigam Jeff?De Pons Mary?Shimoyama Melinda?R?Dwinell Elizabeth?A?Worthey Howard?J?Jacob 《Human genomics》2014,8(1):17
Background
Biological systems are exquisitely poised to respond and adjust to challenges, including damage. However, sustained damage can overcome the ability of the system to adjust and result in a disease phenotype, its underpinnings many times elusive. Unraveling the molecular mechanisms of systems biology, of how and why it falters, is essential for delineating the details of the path(s) leading to the diseased state and for designing strategies to revert its progression. An important aspect of this process is not only to define the function of a gene but to identify the context within which gene functions act. It is within the network, or pathway context, that the function of a gene fulfills its ultimate biological role. Resolving the extent to which defective function(s) affect the proceedings of pathway(s) and how altered pathways merge into overpowering the system's defense machinery are key to understanding the molecular aspects of disease and envisioning ways to counteract it. A network-centric approach to diseases is increasingly being considered in current research. It also underlies the deployment of disease pathways at the Rat Genome Database Pathway Portal. The portal is presented with an emphasis on disease and altered pathways, associated drug pathways, pathway suites, and suite networks.Results
The Pathway Portal at the Rat Genome Database (RGD) provides an ever-increasing collection of interactive pathway diagrams and associated annotations for metabolic, signaling, regulatory, and drug pathways, including disease and altered pathways. A disease pathway is viewed from the perspective of networks whose alterations are manifested in the affected phenotype. The Pathway Ontology (PW), built and maintained at RGD, facilitates the annotations of genes, the deployment of pathway diagrams, and provides an overall navigational tool. Pathways that revolve around a common concept and are globally connected are presented within pathway suites; a suite network combines two or more pathway suites.Conclusions
The Pathway Portal is a rich resource that offers a range of pathway data and visualization, including disease pathways and related pathway suites. Viewing a disease pathway from the perspective of underlying altered pathways is an aid for dissecting the molecular mechanisms of disease.64.
Seth M Barribeau Ben M Sadd Louis du Plessis Mark JF Brown Severine D Buechel Kaat Cappelle James C Carolan Olivier Christiaens Thomas J Colgan Silvio Erler Jay Evans Sophie Helbing Elke Karaus H Michael G Lattorff Monika Marxer Ivan Meeus Kathrin N?pflin Jinzhi Niu Regula Schmid-Hempel Guy Smagghe Robert M Waterhouse Na Yu Evgeny M Zdobnov Paul Schmid-Hempel 《Genome biology》2015,16(1)
65.
The structure of pseudorabies virus (PRV) capsids isolated from the nucleus of infected cells and from PRV virions was determined by cryo-electron microscopy (cryo-EM) and compared to herpes simplex virus type 1 (HSV-1) capsids. PRV capsid structures closely resemble those of HSV-1, including distribution of the capsid vertex specific component (CVSC) of HSV-1, which is a heterodimer of the pUL17 and pUL25 proteins. Occupancy of CVSC on all PRV capsids is near 100%, compared to ~ 50% reported for HSV-1 C-capsids and 25% or less that we measure for HSV-1 A- and B-capsids. A PRV mutant lacking pUL25 does not produce C-capsids and lacks visible CVSC density in the cryo-EM-based reconstruction. A reconstruction of PRV capsids in which green fluorescent protein was fused within the N-terminus of pUL25 confirmed previous studies with a similar HSV-1 capsid mutant localizing pUL25 to the CVSC density region that is distal to the penton. However, comparison of the CVSC density in a 9-Å-resolution PRV C-capsid map with the available crystal structure of HSV-1 pUL25 failed to find a satisfactory fit, suggesting either a different fold for PRV pUL25 or a capsid-bound conformation for pUL25 that does not match the X-ray model determined from protein crystallized in solution. The PRV capsid imaged within virions closely resembles C-capsids with the addition of weak but significant density shrouding the pentons that we attribute to tegument proteins. Our results demonstrate significant structure conservation between the PRV and HSV capsids. 相似文献
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JP Valde LG Lawson A Lindberg JF Agger H Saloniemi O Østerås 《Acta veterinaria Scandinavica》2004,45(4):201-210
Data from the national dairy cow recording systems during 1997 were used to calculate lactation-specific cumulative risk of mastitis treatments and cumulative risk of removal from the herds in Denmark, Finland Norway and Sweden. Sweden had the lowest risk of recorded mastitis treatments during 305 days of lactation and Norway had the highest risk. The incidence risk of recorded mastitis treatments during 305 days of lactation in Denmark, Finland, Norway and Sweden was 0.177, 0.139, 0.215 and 0.127 for first parity cows and 0.228, 0.215, 0.358 and 0.204 for parities higher than three, respectively. The risk of a first parity cow being treated for mastitis was almost 3 times higher at calving in Norway than in Sweden. The period with the highest risk for mastitis treatments was from 2 days before calving until 14 days after calving and the highest risk for removal was from calving to 10 days after calving in all countries.The study clearly demonstrated differences in bovine mastitis treatment patterns among the Nordic countries. The most important findings were the differences in treatment risks during different lactations within each country, as well as differences in strategies with respect to the time during lactation mastitis was treated. 相似文献
68.
TIMOTHY J. CURRAN ROBYN L. BROWN EMILEE EDWARDS KRISTINA HOPKINS CATHERINE KELLEY ELIZABETH MCCARTHY ERIN POUNDS RENATA SOLAN JAMI WOLF 《Austral ecology》2008,33(4):451-461
Abstract Cyclones cause profound immediate impacts on tropical rainforest trees, including defoliation, limb loss, snapping of stems and uprooting. Some studies have shown that plant functional traits such as tree size, buttress roots and wood density are correlated with these forms of cyclone damage. On 20 March 2006, Severe Tropical Cyclone Larry crossed the north Queensland coast and proceeded inland across the Atherton Tablelands, impacting the critically endangered Mabi Type 5b rainforest. We investigated the effects of Cyclone Larry on common tree species by categorizing damage to trees as uprooted, snapped, limbs damaged (light, moderate, severe) or upright and estimating levels of defoliation. Damage was then related to functional traits including tree size, presence of buttress roots, wood density, and leaf size and strength. Levels of damage differed between species. Tree size (diameter at breast height) and the presence of buttress roots were not related to damage levels. Wood density was significantly negatively correlated to proportion of trees with snapped stems and significantly positively correlated with the proportion of trees upright with no or light limb damage. Levels of defoliation were significantly related to leaf strength (specific leaf area – SLA) and to leaf width, but not other components of leaf size (area or length) or petiole length. Species with high wood density and low SLA (e.g. Argyrodendron spp.) were found to have high cyclone resistance, the ability to resist damage, while species with low wood density and high SLA (e.g. Dendrocnide photinophylla) exhibited low resistance. However, traits related to low resistance are also those linked to rapid growth and high cyclone resilience, the ability to recover from damage, so it is unlikely that the Mabi forest will experience long‐term changes in floristic composition following Cyclone Larry. 相似文献
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