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Rhida M'Rad Marek Sanak Georges Deschenes Jing Zhou Catherine Bonaiti-Pellie Laurent Holvoet-Vermaut Solange Heuertz Marie-Claire Gubler Michel Broyer Jean-Pierre Grunfeld Karl Tryggvason Marie-Claude Hors-Cayla 《Human genetics》1992,90(4):420-426
Thirty one families with Alport syndrome including 3 families with associated syndromes were studied. The location of the COL4A5 gene, responsible for the Alport syndrome, was determined by linkage analysis with eight probes of the Xq arm and by a radiation hybrid panel. Concordant data indicated the localization of the Alport gene between DXS17 and DXS11. Four deletions and one single base mutation of the COL4A5 gene were detected. Homogeneity tests failed to show any evidence of genetic heterogeneity superimposed on clinical heterogeneity for ophthalmic signs and end-stage renal disease age. 相似文献
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取食雄蜂蛹粉对龟纹瓢虫和异色瓢虫卵黄发生的影响 总被引:1,自引:1,他引:0
本文比较了龟纹瓢虫Proylea japonica和异色瓢虫Harmonia axyridis取食蚜虫和取食雄蜂蛹粉时的卵黄发生情况.当取食雄蜂蛹粉时,体内卵黄蛋白出现迟,积累速度慢,产卵前期长.但用保幼激素类似物ZR512点滴处理后则能达到与食蚜对照相当的水平.ZR512对取食雄蜂蛹粉瓢虫的作用显著大于取食岈虫者.进一步的研究表明,ZR512能促进这两种瓢虫取食雄蜂蛹粉,但对成虫的体重没有明显的影响.因此推论,雄蜂蛹粉基本能够满足这两种瓢虫生殖的营养需要,但对其内分泌有一定的影响,使瓢虫处于类似生殖滞育的状态.本文根据不同食物条件对卵黄蛋白发生的影响不同,建议用卵黄蛋白的量作为生理指标,以快速初步筛选和评估人工饲料. 相似文献
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The trk tyrosine protein kinase mediates the mitogenic properties of nerve growth factor and neurotrophin-3. 总被引:45,自引:0,他引:45
C Cordon-Cardo P Tapley S Q Jing V Nanduri E O'Rourke F Lamballe K Kovary R Klein K R Jones L F Reichardt 《Cell》1991,66(1):173-183
The product of the trk proto-oncogene encodes a receptor for nerve growth factor (NGF). Here we show that NGF is a powerful mitogen that can induce resting NIH 3T3 cells to enter S phase, grow in semisolid medium, and become morphologically transformed. These mitogenic effects are absolutely dependent on expression of gp140trk receptors, but do not require the presence of the previously described low affinity NGF receptor. gp140trk also serves as a receptor for the related factor neurotrophin-3 (NT-3), but not for brain-derived neurotrophic factor. Both NGF and NT-3 induce the rapid phosphorylation of gp140trk receptors and the transient expression of c-Fos proteins. However, NT-3 appears to elicit more limited mitogenic responses than NGF. These results indicate that the product of the trk proto-oncogene is sufficient to mediate signal transduction processes induced by NGF and NT-3, at least in proliferating cells. 相似文献
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The trkB tyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3. 总被引:48,自引:0,他引:48
R Klein V Nanduri S A Jing F Lamballe P Tapley S Bryant C Cordon-Cardo K R Jones L F Reichardt M Barbacid 《Cell》1991,66(2):395-403
trkB is a tyrosine protein kinase gene highly related to trk, a proto-oncogene that encodes a receptor for nerve growth factor (NGF) and neurotrophin-3 (NT-3). trkB expression is confined to structures of the central and peripheral nervous systems, suggesting it also encodes a receptor for neurotrophic factors. Here we show that brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of gp145trkB, one of the receptors encoded by trkB. BDNF and NT-3 can induce DNA synthesis in quiescent NIH 3T3 cells that express gp145trkB. Cotransfection of plasmids encoding gp145trkB and BDNF or NT-3 leads to transformation of recipient NIH 3T3 cells. In these assays, BDNF elicits a response at least two orders of magnitude higher than NT-3. Finally, 125I-NT-3 binds to NIH 3T3 cells expressing gp145trkB; binding can be competed by NT-3 and BDNF but not by NGF. These findings indicate that gp145trkB may function as a neurotrophic receptor for BDNF and NT-3. 相似文献
19.
The trk proto-oncogene encodes a receptor for nerve growth factor. 总被引:127,自引:0,他引:127
Two classes of receptors with distinct affinities for nerve growth factor (NGF) have been identified. The low affinity receptor (Kd approximately 10(-9) to 10(-8) M) is a cysteine-rich glycoprotein encoded by the previously characterized LNGFR gene. The structural nature of the high affinity receptor (Kd approximately 10(-11) to 10(-10) M) has yet to be established. In this study we show that the product of the human trk proto-oncogene (gp140trk) binds NGF with high affinity. Moreover, NGF could be chemically cross-linked to the endogenous gp140trk present in rat PC12 pheochromocytoma cells as well as to gp140trk ectopically expressed in mouse fibroblasts and in insect Sf9 cells. High affinity binding of NGF to gp140trk can occur in the absence of low affinity LNGFR receptors, at least in nonneural cells. Addition of NGF to PC12 cells elicits rapid phosphorylation of gp140trk on tyrosine residues and stimulates its tyrosine kinase activity. These results indicate that gp140trk is a functional NGF receptor that mediates at least some of the signal transduction processes initiated by this neurotrophic factor. 相似文献
20.
本文对高胆固醇血症家兔红细胞在交、直流电场中的电泳行为进行了多指标测定,结果显示,高脂组与正常组相比红细胞聚集能力增强,变形能力及膜流动性下降,表明高脂血症可能较易导致血栓形成。中药有效成分8501有对抗高脂引起的红细胞上述改变的作用,提示8501可能对血栓和动脉粥样硬化斑块形成有防治作用。 相似文献