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We tested the hypotheses that relative activity of the less efficient alternative oxidase (AOX) path changes with diurnal temperature changes, and thus changes carbon use efficiency with temperature. The activities of the alternative and cytochrome oxidase (COX) paths in plant tissues of three species were determined by measuring 18O/16O discrimination and total respiration from 17 to 36 °C. A new, more accurate method for calculating oxygen uptake rate from the mass spectrometry data was developed. Total carbon use efficiency was calculated from the ratio of respiratory heat and CO2 rates measured from 10 to 35 °C. Oxygen isotope discrimination (22.9 ± 0.4‰) and AOX participation were invariant with temperature in leaf tissue of Cucurbita pepo , Nicotiana sativa and Vicia faba , thus falsifying the first part of the hypothesis. Stress responses of respiration at the temperature extremes limited the range for which carbon use efficiency could be accurately measured to 15–30 °C in N. sativa , to 10–25 °C in C. pepo and to 20–30 °C in V. faba . Carbon-use efficiency was invariant at these temperatures in these species, demonstrating that changes in other pathways that would vary carbon-use efficiency were also invariant with temperature.  相似文献   
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ABSTRACT Changes in resource selection associated with human predation risk may alter elk distributions and availability for harvest. We used Global Positioning System data collected from telemetered female elk (Cervus elaphus) to evaluate effects of refuges (areas where hunting was prohibited), spatial variation in hunting risk, and landscape attributes on resource selection within an established Greater Yellowstone Area, USA, winter range. We also evaluated elk distributions during and outside of a late-season hunting period. Refuge areas and landscape attributes such as habitat type and snow water equivalents (SWE) affected resource selection. Elk selection for flat grasslands increased as SWE increased, likely because these areas were windswept, leaving grasses exposed for foraging. Elk distributions differed during hunting and no-hunting periods. During the hunting period, elk shifted to privately owned refuge areas and the estimated odds of elk occupying refuge areas more than doubled. Risk-driven changes in resource selection resulted in reduced availability of elk for harvest. Elk selection for areas where hunting is prohibited presents a challenge for resource managers that use hunting as a tool for managing populations and influences grazing patterns on private ranchlands.  相似文献   
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ABSTRACT We evaluated survival of elk (Cervus elaphus) calves on 2 contrasting study areas in north-central Idaho, USA, from 1997 to 2004. Recruitment was modest (>30 calves:100 F [calves of either sex: F elk 1 yr old]) and stable on the South Fork study area and low (<20 calves:100 F) and declining on the Lochsa study area. The primary proximate cause of calf mortality on both study areas was predation by black bears (Ursus americanus) and mountain lions (Puma concolor). We experimentally manipulated populations of black bears and mountain lions on a portion of each study area. Black bear harvest (harvest density/600km2) initially doubled on the Lochsa treatment after manipulating season bag limits. Mountain lion harvest also increased by 60% but varied widely during the manipulation period. Harvest seasons were closed for black bears and mountain lions on the treatment portion of the South Fork study area. Using the Andersen—Gill formulation (A-G) of the Cox proportional hazards model, we examined effects of landscape structure, predator harvest levels, and biological factors on summer calf survival. We used Akaike's Information Criterion (AICc) and multimodel inference to assess some potentially useful predictive factors relative to calf survival. We generated risk ratios for both the best models and for model-averaged coefficients. Our models predicted that calf survival was influenced by biological factors, landscape surrounding calf locations, and predator harvest levels. The model that best explained mortality risk to calves on the Lochsa included black bear harvest (harvest density/600 km2), estimated birth mass of calves, and percentage of shrub cover surrounding calf locations. Incorporating a shrub X time interaction allowed us to correct for nonproportionality and detect that effect of shrub cover was only influential during the first 14 days of a calf's life. Model-averaging indicated that estimated birth mass of calves and black bear harvest were twice as important as the next variables, but age of calves at capture was also influential in calf survival. The model that best explained mortality risk to calves on the South Fork included black bear harvest, age of calves at capture, and gender of calves. Model-averaging indicated that age at capture and black bear harvest were twice as important as the next variable, forest with 33–66% canopy cover (Canopy 33–66). Risk to calves decreased when calves occupied areas with more of this forest cover type. Model-averaging also indicated that increased mountain lion harvest lowered calf mortality risk 4% for every 1-unit increase in lion harvest (harvest density/600 km2) but was lower (<25%) in importance compared to age at capture and black bear harvest. Our results suggest that levels of predator harvest, and presumably predator density, resource limitations expressed through calf birth mass, and habitat structure had substantial effects on calf survival. Our results can be generalized to other areas where managers are dealing with low calf elk recruitment. However, because factors vary spatially, a single management strategy applied in different areas will probably not have the same effect on calf survival.  相似文献   
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The life‐history strategies of some species make them strong candidates for rapid exploitation of novel habitat under new climate regimes. Some early‐responding species may be considered invasive, and negatively impact on ‘naïve’ ecosystems. The barrens‐forming sea urchin Centrostephanus rodgersii is one such species, having a high dispersal capability and a high‐latitude range margin limited only by a developmental temperature threshold. Within this species’ range in eastern Australian waters, sea temperatures have increased at greater than double the global average rate. The coinciding poleward range extension of C. rodgersii has caused major ecological changes, threatening reef biodiversity and fisheries productivity. We investigated microsatellite diversity and population structure associated with range expansion by this species. Generalized linear model analyses revealed no reduction in genetic diversity in the newly colonized region. A ‘seascape genetics’ analysis of genetic distances found no spatial genetic structure associated with the range extension. The distinctive genetic characteristic of the extension zone populations was reduced population‐specific FST, consistent with very rapid population expansion. Demographic and genetic simulations support our inference of high connectivity between pre‐ and post‐extension zones. Thus, the range shift appears to be a poleward extension of the highly‐connected rangewide population of C. rodgersii. This is consistent with advection of larvae by the intensified warm water East Australian current, which has also increased Tasmanian Sea temperatures above the species’ lower developmental threshold. Thus, ocean circulation changes have improved the climatic suitability of novel habitat for C. rodgersii and provided the supply of recruits necessary for colonization.  相似文献   
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Recent attempts at projecting climate change impacts on biodiversity have used the IUCN Red List Criteria to obtain estimates of extinction rates based on projected range shifts. In these studies, the Criteria are often misapplied, potentially introducing substantial bias and uncertainty. These misapplications include arbitrary changes to temporal and spatial scales; confusion of the spatial variables; and assume a linear relationship between abundance and range area. Using the IUCN Red List Criteria to identify which species are threatened by climate change presents special problems and uncertainties, especially for shorter‐lived species. Responses of most species to future climate change are not understood well enough to estimate extinction risks based solely on climate change scenarios and projections of shifts and/or reductions in range areas. One way to further such understanding would be to analyze the interactions among habitat shifts, landscape structure and demography for a number of species, using a combination of models. Evaluating the patterns in the results might allow the development of guidelines for assigning species to threat categories, based on a combination of life history parameters, characteristics of the landscapes in which they live, and projected range changes.  相似文献   
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Odor identification: perceptual and semantic dimensions   总被引:8,自引:8,他引:0  
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