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961.
962.
A substitution mutation in OsCCD7 cosegregates with dwarf and increased tillering phenotype in rice 总被引:2,自引:0,他引:2
KRISHNANAND P. KULKARNI CHANDRAPAL VISHWAKARMA SARADA P. SAHOO JOHN M. LIMA MANOJ NATH PRASAD DOKKU RAJESH N. GACCHE TRILOCHAN MOHAPATRA S. ROBIN N. SARLA M. SESHASHAYEE ASHOK K. SINGH KULDEEP SINGH NAGENDRA K. SINGH R. P. SHARMA 《Journal of genetics》2014,93(2):389-401
Dwarf plant height and tillering ability are two of the most important agronomic traits that determine the plant architecture, and have profound influence on grain yield in rice. To understand the molecular mechanism controlling these two traits, an EMS-induced recessive dwarf and increased tillering1 (dit1) mutant was characterized. The mutant showed proportionate reduction in each internode as compared to wild type revealing that it belonged to the category of dn-type of dwarf mutants. Besides, exogenous application of GA3 and 24-epibrassinolide, did not have any effect on the phenotype of the mutant. The gene was mapped on the long arm of chromosome 4, identified through positional candidate approach and verified by cosegregation analysis. It was found to encode carotenoid cleavage dioxygenase7 (CCD7) and identified as an allele of htd1. The mutant carried substitution of two nucleotides CC to AA in the sixth exon of the gene that resulted in substitution of serine by a stop codon in the mutant, and thus formation of a truncated protein, unlike amino acid substitution event in htd1. The new allele will facilitate further functional characterization of this gene, which may lead to unfolding of newer signalling pathways involving plant development and architecture. 相似文献
963.
DANIEL JAMES WHITE JONCI NIKOLAI WOLFF MELANIE PIERSON NEIL JOHN GEMMELL 《Molecular ecology》2008,17(23):4925-4942
Mitochondrial DNA (mtDNA) is a pivotal tool in molecular ecology, evolutionary and population genetics. The power of mtDNA analyses derives from a relatively high mutation rate and the apparent simplicity of mitochondrial inheritance (maternal, without recombination), which has simplified modelling population history compared to the analysis of nuclear DNA. However, in biology things are seldom simple, and advances in DNA sequencing and polymorphism detection technology have documented a growing list of exceptions to the central tenets of mitochondrial inheritance, with paternal leakage, heteroplasmy and recombination now all documented in multiple systems. The presence of paternal leakage, recombination and heteroplasmy can have substantial impact on analyses based on mtDNA, affecting phylogenetic and population genetic analyses, estimates of the coalescent and the myriad of other parameters that are dependent on such estimates. Here, we review our understanding of mtDNA inheritance, discuss how recent findings mean that established ideas may need to be re‐evaluated, and we assess the implications of these new‐found complications for molecular ecologists who have relied for decades on the assumption of a simpler mode of inheritance. We show how it is possible to account for recombination and heteroplasmy in evolutionary and population analyses, but that accurate estimates of the frequencies of biparental inheritance and recombination are needed. We also suggest how nonclonal inheritance of mtDNA could be exploited, to increase the ways in which mtDNA can be used in analyses. 相似文献
964.
RICHARD LINDROTH SHERRY ROTH ERIC KRUGER JOHN VOLIN & PATRICK KOSS 《Global Change Biology》1997,3(3):279-289
We investigated the effects of long-term CO2 enrichment on foliar chemistry of quaking aspen ( Populus tremuloides ) and the consequences of chemical changes for performance of the gypsy moth ( Lymantria dispar ) and susceptibility of the gypsy moth to a nucleopolyhedrosis virus (NPV). Foliage was collected from outdoor open-top chambers and fed to insects in a quarantine rearing facility. Under enriched CO2 , levels of leaf nitrogen declined marginally, levels of starch and phenolic glycosides did not change, and levels of condensed tannins increased. Long-term bioassays revealed reduced growth (especially females), prolonged development and increased consumption in larvae fed high-CO2 foliage but no significant differences in final pupal weights or female fecundity. Short-term bioassays showed weaker, and sex-specific, effects of CO2 treatment on larval performance. Correlation analyses revealed strong, negative associations between insect performance and phenolic glycoside concentrations, independent of CO2 treatment. Larval susceptibility to NPV did not differ between CO2 treatments, suggesting that effects of this natural enemy on gypsy moths are buffered from CO2 -induced changes in foliar chemistry. Our results emphasize that the impact of enriched CO2 on plant–insect interactions will be determined not only by how concentrations of plant compounds are altered, but also by the relevance of particular compounds for insect fitness. This work also underscores the need for studies of genetic variation in plant responses to enriched CO2 and long-term population-level responses of insects to CO2 -induced changes in host quality. 相似文献
965.
JOHN A'BROOK 《The Annals of applied biology》1981,97(1):11-15
Some preliminary associations are reported between monthly totals of cereal aphids (Metopolophium dirhodum, Rhopalosiphum insertum, R. padi and Sitobion avenae) caught in suction traps and weather data. Catches at 1.2 and 12.2 m during the summer and autumn flight peaks from 1969 to 1979 were compared with combinations of prior monthly totals of rainfall and accumulated day-degree temperatures. The best models fitting these results are reported and proposed for testing against future data. The possible relevance of these models, for forecasting whether measures for the control of barley yellow dwarf virus in autumn-sown cereals are necessary in any year, are discussed. 相似文献
966.
1. Allozyme electrophoresis was used to describe the genetic structure of Helicopsyche borealis caddisflies collected from three sites in each of three streams in northern California, U.S.A.: the Rice Fork of the Eel River; Big Sulphur Creek; and Alameda Creek. Between 7 and 11km separated adjacent sites within these three streams. Helicopsyche borealis from three additional streams in eastern North America (Christiana Creek, Indiana; Byrd's Mill Creek, Oklahoma; Susquehanna River, Pennsylvania) were also analysed electrophoretically to address taxonomic questions that arose during the study. 2. Four genetically distinct groups of individuals were identified (i.e. Helicopsyche types A, B, C, and D). Lack of interbreeding between sympatric groups (as evidenced by fixed allelic differences) and large genetic differences (mean Nei's genetic distances = 0.396–0.693) indicate that these four groups of Helicopsyche were actually reproductively isolated species rather than genetic variants of a single species. 3. Occurrence of Helicopsyche type A at multiple sites permitted an analysis of spatial variation in genetic structure. Within a drainage basin, small differences in allele frequencies were observed among sites in the Rice Fork and Big Sulphur Creek, but not in Alameda Creek. Larger genetic differences were found among sites in separate drainage basins. Genetic distances (Nei's) between Helicopsyche type A from California and from eastern North America sites (mean = 0.236) were greater than interpopulation differences commonly observed for insects, which suggests that Helicopsyche type A from California may represent a different species than Helicopsyche type A from eastern North America. 4. Geographical and taxonomic differences observed in this study underscore the importance of understanding both population structure and genetic relationships among populations in the design and interpretation of stream faunal studies. 相似文献
967.
GABRIELLE M. OSULLIVAN ZLATIBOR M. VELICKOVIC MICHELLE W. KEIR JANET L. MACPHERSON JOHN E.J. RASKO 《Cytotherapy》2019,21(12):1258-1273
Cell and gene therapy products are rapidly being integrated into mainstream medicine. Developing global capability will facilitate broad access to these novel therapeutics. An initial step toward achieving this goal is to understand cell and gene therapy manufacturing capability in each region. We conducted an academic survey in 2018 to assess cell and gene therapy manufacturing capacity in Australia and New Zealand. We examined the following: the number and types of cell therapy manufacturing facilities; the number of projects, parallel processes and clinical trials; the types of products; and the manufacturing and quality staffing levels. It was found that Australia and New Zealand provide diverse facilities for cell therapy manufacturing, infrastructure and capability. Further investment and development will enable both countries to make important decisions to meet the growing need for cell and gene therapy and regenerative medicine in the region. 相似文献
968.
JOHN R. PANNELL DARREN J. OBBARD RICHARD J. A. BUGGS 《Biological journal of the Linnean Society. Linnean Society of London》2004,82(4):547-560
The evolutionary success of polyploidy most directly requires the ability of polyploid individuals to reproduce and transmit their genes to subsequent generations. As a result, the sexual system (i.e. the mating system and the sex allocation of a species) will necessarily play a key role in determining the fate of a new polyploid lineage. The effects of the sexual system on the evolution of polyploidy are complex and interactive. They include both aspects of the genetic system, the genetic load maintained in a population and the ecological context in which selection takes place. Here, we explore these complexities and review the empirical evidence for several potentially important genetic and ecological interactions between ploidy and the sexual system in plants. We place particular emphasis on work in our laboratory on the European annual plant Mercurialis annua , which offers promising scope for detailed investigations on this topic. M. annua forms a polyploid complex that varies in its sexual system from dioecy (separate sexes) through androdioecy (males and hermaphrodites) to functional hermaphroditism. © 2004 The Linnean Society of London, Biological Journal of the Linnean Society , 2004, 82 , 547–560. 相似文献
969.
970.
NICOLE L. GUNTER JOHN M. LEAVENGOOD JUSTIN S. BARTLETT ERIC G. CHAPMAN STEPHEN L. CAMERON 《Systematic Entomology》2013,38(3):626-636
We provide the first molecular phylogeny of the clerid lineage (Coleoptera: Cleridae, Thanerocleridae) within the superfamily Cleroidea to examine the two most recently proposed hypotheses of higher level classification. Phylogenetic relationships of checkered beetles were inferred from approximately ~5000 nt of both nuclear and mitochondrial rDNA (28S, 16S and 12S) and the mitochondrial protein‐coding gene COI. A worldwide sample of ~70 genera representing almost a quarter of generic diversity of the clerid lineage was included and phylogenies were reconstructed using Bayesian and Maximum Likelihood approaches. Results support the monophyly of many proposed subfamilies but were not entirely congruent with either current classification system. The subfamilial relationships within the Cleridae are resolved with support for three main lineages. Tillinae are supported as the sister group to all other subfamilies within the Cleridae, whereas Thaneroclerinae, Korynetinae and a new subfamily formally described here, Epiclininae subf.n ., form a sister group to Clerinae + Hydnocerinae. 相似文献