Number of ovarioles and fecundity in the black bean aphid, Aphis fabae

The number of ovarioles in the gonads of the host alternating aphid, Aphis fabae Scop., is not constant. The apterous generations on spindle, emigrants and initial apterous colonists of the secondary host each have a characteristic range of number of ovarioles; subsequent generations in the yearly life cycle have twelve, except for alate exules which have a range and the lowest average number of ovarioles. Ovariole number is programmed and is not a function of adult weight or food quality. The generations with the largest number of ovarioles have the greatest fecundity, highest reproductive rate and smallest offspring, and develop early in the season when the environment is favourable. Later generations have a low fecundity and proportionately more fat, a possible adaptation to the harsher environment these aphids experience.

---

Zusammenfassung Die Anzahl der Ovariolen in den Eierstöcken ist bei der wirtswechselnden Bohnenlaus, Aphis fabae, nicht konstant. Bei Läusen der ungeflügelten Generationen auf Pfaffenhütchen, sowie bei Migrantes und den ersten apteren Aphiden auf dem Sekundärwirt variiert die Anzahl der Ovariolen in einem charakteristischen Bereich. Die folgenden Generationen im jährlichen Zyklus haben stets zwölf Ovariolen, ausgenommen alate Exsules, bei denen ebenfalls ein charakteristischer Bereich nachzuweisen war, allerdings mit einem sehr niedrigen Durchschnitt. Die Anzahl der Ovariolen ist programmiert. Ihre Anzahl ist konstant und nicht vom Gewicht der Imagines oder der Nahrungsqualität abhängig. Die Generationen mit der höchsten Anzahl von Ovariolen sind am fruchtbarsten, sie haben die höchste Reproduktionsrate und gebären die kleinsten Läuse. Sie entwickeln sich frühzeitig, wenn die Bedingungen günstig sind. Spätere Generationen zeigen eine niedrigere Fruchtbarkeit, lagern jedoch verhältnismäßig mehr Fett ein, wahrscheinlich eine Anpassung an die schlechteren Bedingungen, denen diese Läuse ausgesetzt sind.

     

Crystallographic and molecular-orbital studies on the geometry of antifolate drugs.

In the common dihydrofolate reductase inhibitors an amino substituent replaces the pteridine carbonyl oxygen atom of folates, with altered hydrogen-bonding properties and size. Flexibility in the amino groups could facilitate enzyme binding. Studies of cycloguanil hydrochloride by neutron diffraction show both in-plane and out-of-plane deformation of amino groups. Molecular-orbital calculations ab initio on 2,4-diamino-5-methylpyrimidinium cation confirm that the 4-amino group is readily deformable. The 2,4-diaminoquinazoline structure is reported. Atomic co-ordinates, thermal parameters, bond distances and bond angles for cycloguanil and 2,4-diaminoquinazoline have been deposited as Supplementary Publication SUP 50108 (13 pages) at the British Library Lending Division, Boston Spa. Wetherby, West Yorkshire LS23, 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1978) 169, 5.     

Incomplete influenza virus: partial functional complementation as revealed by hemadsorbing cell count test.

In MDCK cells inoculated with an appropriate dilution of influenza virus, single hemadsorbing cells could be counted 8 h postinfection against a background of nonadsorbing cells. Standard virus preparation exhibited a linear relationship between the virus dilution and the number of hemadsorbing cells. With incomplete virus preparations obtained by passages of undiluted virus in chicken embryo, the dependence was nonlinear. A ts mutant (ts-29) of A/FPV/Weybridge (Hav1 Neq1) failed to convert MDCK cells into a hemadsorbing state at 42 degrees C. The ability of ts-29 to produce hemadsorbing cells could be rescued by incomplete wild-type virus. The capacity of incomplete virus for this partial functional complementation was inactivated by UV irradiation with one-hit kinetics. The size of the target was estimated to be 5.5 times smaller than that of the virus genome. The results suggest that at least some of the influenza virus genes in defective interfering particles are functional.     

Improved Filtration Technique for Concentrating and Harvesting Bacteria

An improved technique is described for the filtrative concentration and harvesting of bacterial cultures. A pleated tangential flow filtration unit containing 1,000 cm² of 0.2-μm-pore-size microporous membrane was used to rapidly (30 to 50 min) reduce the volume of 5 liters of bacterial culture of approximately 10⁹ cells per ml to 0.2 to 0.5 liters of concentrated bacterial suspension. The effects of cell concentration, filtration pressure, and tangential flow rate were examined with respect to the rate of concentration and cell viability. Recovery efficiencies were between 60 and 75%, with no apparent impairment of organism viability. Cell concentration exerted the predominant effect on the filtration rate.     

Physiological Adaptations in Response to Environmental Stress During an N2-Fixing Anabaena Bloom

Anabaena spiroides has the ability to maintain intense biomass production for extensive periods in the epilimnion of a small eutrophic lake characterized by conditions shown to cause photooxidative death in a number of other phytoplankton. By the enhancement of carotenoid synthesis chlorophyll a was protected from photooxidation and prevented from catalyzing other photooxidative reactions within the cells. By temporally separating CO₂ and N₂ fixation, maximum utilization of photosynthetically active radiation was achieved. Because CO₂ fixation was more sensitive than N₂ fixation to a high oxygen concentration, the former was maximized during morning hours, before the afternoon buildup of dissolved oxygen. The diurnal partitioning of carbon and N₂ fixation has two additional advantages; possible competition for reductant-generating compounds is minimized, and adequate endogenous pools of carbon skeletons are assured to accept newly fixed ammonia. Hence, Anabaena, far from undergoing photooxidative death, appears to utilize a physiological strategy which allows optimization of radiant energy use for reductive processes and dominance of surface waters and shading of deeper phytoplankton during summer blooms.     

Development of Gametophytes in Fokienia Cupressaceae

The structure of the ovule and the development of the gametophytes in Fokienia, an endemic genus of Cupressaceae are described in some detail. Two wings, one small and one large, are developed along the micropylar end of the ovule and two resin canals are present in each of them. The material collected in the middle of April was already pollinated and the pollen began to germinate on the nucellus. The sterile cell, tube cell and spermatogenous cell have been formed in the tube in the first collection of April 17. At the end of June the division of the spermatogenous cell results in two sperms of Similar size and shape and the division plane is usually parallel to long axis of the pollen tube. Both sperms are effective in fertilization. 4096 free nuclei (actual counting, 3733—4224 ones) are produced through 12 times of repeated divisions of the functional megaspore, then cell walls appear among the free nuclei and cellular female gametophyte is formed. The number of archegonia varies from 6 to 16, mostly 9–12. The archegonial complex is enclosed by 2–3 layers of jacket cells. The neck cells are usually 4 in number, arranged in 1–2 layers. The central cell divides and results in the formation of one ventral canal nucleus and one egg nucleus. Fertilization takes place in the middle of the archegonium. The development of the gametophytes of Fokienia is more or less similar to that of Sabina.     

Propidium iodide as a probe for the study of chromatin thermal denaturationin situ

Summary The possibility of using propidium iodide, a phenanthridinic fluorochrome specific for double-stranded nucleic acids, for the study of chromatin thermal denaturationin situ has been examined. Smears of lymphocytes and hepatocyte nuclei from 15-day-old rats were fixed in acetic acid-ethanol (13 v/v), treated with RNAse and submitted to different protein extraction procedures, namely, incubation with pepsin, trypsin and sodium chloride.Denaturation experiments were performed in Sörensen buffer at pH 7.4 containing 10% formamide at temperatures between 27 and 95°C. The samples were stained with propidium iodide and mounted in buffer or glycerol. Measurements were performed with a microfluorometer at a wavelength of 546 nm.The results indicate a higher thermostability of lymphocytes as compared to hepatocytes. The denaturation pattern suggests a certain organization complexity of chromatin, better emphasized by the derivative curves which show the presence of at least three fractions with different melting points. After protein extraction, the denaturation curves exhibit a somewhat simplified pattern, with the disappearance of the most stable peak in the derivative curves. The samples mounted in glycerine exhibit a better stability of staining with time, and an increased quantum efficiency of the fluorochrome with regard to those mounted in buffer.These data confirm the importance of protein-DNA interactions in the organization of chromatin and point to some differences, depending on the cell type and on functional activity.     

Early Initiation of Deoxyribonucleic Acid Replication and Shortening of Generation Time Associated with Inhibition of Lateral Wall Formation by Mecillinam

The effects of mecillinam on the growth of rods of the pH-conditional morphology mutant MirM7 was studied. It has been found that mecillinam causes, coincident with transition to coccal shape, a balanced rise in the rate of viable count increase and the rate of macromolecular synthesis which lasts either until the cells enter a stationary growth phase or indefinitely, in the case of continuously diluted cultures. When the antibiotic is removed from cells which have already become coccoid, cells continue to grow at a faster rate until they resume the rod shape. No change in the per-cell rate of protein synthesis has been seen in untreated or mecillinam-treated cells before or after the change in growth rate. Studies with synchronously growing cells have shown that the antibiotic causes a shortening in the I period (initiation of deoxyribonucleic acid replication). Evaluation of the residual divisions in nalidixic acid-treated, exponential-phase cells has shown that mecillinam also shortens the D period (cell division). It is proposed that, in strain MirM7, inhibition of lateral wall elongation by the antibiotic allows the initiation of a new septum, though inhibition is still in progress. The initiation of a new septum is, in turn, responsible for both the early inibition of deoxyribonucleic acid replication and accelerated division. In the parental strain, MirA12, as well as in other sensitive gram-negative rods which divide, become cocci, and stop dividing after addition of the antibiotic, inhibition of lateral wall formation activates a feedback mechanism which prevents insertion of new septa (Satta et al., J. Bacteriol. 142:43-51, 1980). Consequently, no early initiation of deoxyribonucleic acid replication is observed, and the last division allowed by the antibiotic occurs in due time. This negative control is missing in MirM7.     

Genetic Analyses of the Polarity Alleles in Recombinants from Mitochondrial Genetic Crosses

A number of minority recombinant and parental types from a heterosexual cross were analyzed for the omega allele they carry. It was found that recombinant progeny can be omega(-), that minority parental types among the progeny can be omega(+) rather than omega(-), and, finally, that certain of the results suggest that the omega locus may not be at the proximal end of the mitochondrial genetic map (Bolotin et al., 1971; Grivell et al., 1973) but rather may lie between the [cap1-r/cap-s] and [ery1-r/ery-s] loci.