AN ADDITIONAL SPECIMEN OF FORKED FROND OF PROTOBLECHNUM WONGII HALLE

In a recently published account entitled “On a forked frond of Protoblechnumwongii Halle”,Dr.Sze has made known an interesting specimen of a forkedfrond of Protoblechnum wongii Halle found from the Shihhotze Series of S.E.     

中缅树鼩形态性状的遗传制约性初步研究

树鼩是一种在医学和生物学上用途广泛的实验动物遗传资源。主要以上海市肿瘤研究所购自云南昆明地区的35只中缅树鼩及其实验室条件下繁殖的G1代为试验素材,对涉及形态性状遗传制约性的一些指标进行分析, 应用Rife-Buranamanas 定律分析中缅树鼩涉及毛色等诸多具有遗传制约性的外貌特征的显隐性关系,得出中缅树鼩典型的外貌特征为野生型毛色有季节性红斑、白腹、没有白眼圈、掌底肤色为肉色、耳朵不竖、圆形的尾尖、两侧乳房每对之间的连线分别与体轴线垂直。 Abstract : Tree shrew is a kind of excellent experimental animal resource in medical science and biology . In this paper, 35 Tupaia blangeri chinensises (TBCs) captured from Kunming,Yunnan province were investigated. We analyzed hereditary conditionality index about some morphological characters. According to Rife-Buranamanas law, We analyzed the appearance characteristics with hereditary conditionality including color of fur, orbit etc.. The results showed the following: wild fur with seasonal red spot, white fur of abdomen, non-white orbit, fleshcolor palm, non-cocked ear, round tip tail, and the line between the breasts of both sides in a vertical position with axis line.     

A multivariate morphometric study on Corylus sieboldiana

Previous taxonomic treatments in Russia and China have considered Corylus mandshurica and C. sieboldiana to be distinct as independent species. A morphometric analysis was conducted to determine if the morphological differentiation from these taxa warrants specific taxonomic recognition with a large sample of field-collected leaves as well as specimens from several herbaria of northeastern Asia. One hundred and fifty two individuals representing Chinese, Korean, and Japanese geographic ranges of the species were scored for 18 morphological characters and the data matrix was used for principal components analysis. The entities that comprise C. sieboldiana complex exhibit widely overlapping ranges in all morphological attributes. The leaf and fruit data may reflect a lack of divergence between taxa. Therefore, we do not regard these to be sufficient for taxonomic splitting of C. mandshurica from C. sieboldiana to warrant the designation of the rank of a species based on morphology. The two taxa were not morphologically well differentiated and their ranges of distribution come together. Therefore, C. mandshurica should be recognized as an infraspecific taxon of C. sieboldiana. C. sieboldiana with short involucres in southern Korea and Japan is often treated as an independent species （C. hallaisanensis） or a variety (C. sieboldiana var. brevirostris), but should be only recognized as a form of C. sieboldiana because this involucral character is highly variable even within the same individual.     

稀有鮈鲫的野生种群结构

稀有鲫(GobiocyprisrarusYeetFu)属鲤科 (Cyprinidae)鱼丹亚科 (Danioninae) 鲫属。       

西藏东南高寒森林大型真菌多样性与植被及环境的关系

通过对西藏东南高寒森林地区3个样地的9个样方两年内较为系统的调查,研究了大型真菌多样性及其与植被类型和大型真菌发生的其他诸多因素之间的关系.基于不同样方物种种类的样方层序聚类分析表明大型真菌的物种发生受植被类型影响较大.多样性指数分析表明急尖长苞冷杉林内的物种丰富度指数和多样性指数大于青冈树和落叶松混交林,后者大于青冈林.应用典范对应分析研究不同植被类型大型真菌物种发生和环境因素的关系,结果表明大型真菌物种的发生受不同环境因子的驱动,其发生是植被类型和环境因素共同作用的结果.     

黄孢原毛皮革菌降解苯胺的工艺研究

通过正交试验优化筛选了适合黄孢原毛皮革菌降解苯胺的适宜培养基和摇瓶培养降解条件。结果表明：其适宜降解的液体培养基组成为：蔗糖20g／L,可溶性淀粉20g／L,(NH4)2SO4l0g／L,Mn^2 lμmol／L,Tween-800．3％,蛋白胨30g／L。适宜降解的摇瓶培养条件为：接种量为20％、pH为7．0、温度为30℃、培养时间为12d．此条件下的苯胺最高降解率可达95．5％。     

结肠腺瘤息肉蛋白突变经PI3K/AKT 信号通路影响犬肾细胞MDCK的细胞粘附

结肠腺瘤息肉蛋白（APC）是一个肿瘤抑制因子,它不仅参与Wnt信号通路的传导,而且对细胞粘附、细胞骨架的组织和迁移等都有影响.APC突变发生于大多数结肠癌中.为了探讨APC突变对细胞粘附的影响及机制,本研究利用细胞粘附实验分析了MDCK-APC-N1和对照MDCK-GFP稳定表达细胞株系的细胞粘附情况.实验结果显示,在MDCK细胞中过表达APC-N1导致细胞-细胞间的粘附减少,细胞-基质间的粘附增加.荧光定量PCR和Western印迹实验表明,在MDCK-APC+N1细胞中,E-cadherin表达水平降低,CD29、P-FAK (Y397)、β-catenin和 P-AKT (T308)表达水平升高. 在MDCK-APC-N1细胞中,敲减β-catenin导致E-cadherin表达量升高,而CD29表达没有明显变化.进一步利用PI3K抑制剂LY294002处理MDCK-APC-N1细胞,结果发现,E-cadherin表达量明显升高,CD29表达量明显降低.这些结果揭示,APC-N1可活化 PI3K/AKT 信号通路,进而改变粘附蛋白E-cadherin和CD29影响细胞粘附.