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排序方式: 共有144条查询结果,搜索用时 15 毫秒
81.
Zeelenberg IS van Maren WW Boissonnas A Van Hout-Kuijer MA Den Brok MH Wagenaars JA van der Schaaf A Jansen EJ Amigorena S Théry C Figdor CG Adema GJ 《Journal of immunology (Baltimore, Md. : 1950)》2011,187(3):1281-1288
Effective antitumor immunotherapy requires the identification of suitable target Ags. Interestingly, many of the tumor Ags used in clinical trials are present in preparations of secreted tumor vesicles (exosomes). In this study, we compared T cell responses elicited by murine MCA101 fibrosarcoma tumors expressing a model Ag at different localizations within the tumor cell in association with secreted vesicles (exosomes), as a nonsecreted cell-associated protein, or as secreted soluble protein. Remarkably, we demonstrated that only the tumor-secreting vesicle-bound Ag elicited a strong Ag-specific CD8(+) T cell response, CD4(+) T cell help, Ag-specific Abs, and a decrease in the percentage of immunosuppressive regulatory T cells in the tumor. Moreover, in a therapeutic tumor model of cryoablation, only in tumors secreting vesicle-bound Ag could Ag-specific CD8(+) T cells still be detected up to 16 d after therapy. We concluded that the localization of an Ag within the tumor codetermines whether a robust immunostimulatory response is elicited. In vivo, vesicle-bound Ag clearly skews toward a more immunogenic phenotype, whereas soluble or cell-associated Ag expression cannot prevent or even delay outgrowth and results in tumor tolerance. This may explain why particular immunotherapies based on these vesicle-bound tumor Ags are potentially successful. Therefore, we conclude that this study may have significant implications in the discovery of new tumor Ags suitable for immunotherapy and that their location should be taken into account to ensure a strong antitumor immune response. 相似文献
82.
Jacobs JF Aarntzen EH Sibelt LA Blokx WA Boullart AC Gerritsen MJ Hoogerbrugge PM Figdor CG Adema GJ Punt CJ de Vries IJ 《Cancer immunology, immunotherapy : CII》2009,58(1):145-151
The occurrence of vitiligo in patients with melanoma is especially reported for patients undergoing immunotherapy. While vitiligo
in these patients is thought to be related to an immune response directed against melanoma cells, solid evidence is lacking.
Here we report local cytotoxic T cell reactivity in three melanoma patients who developed vitiligo, after experimental immunotherapy
using dendritic cell vaccinations. Tetramer analysis showed that vaccine-induced T cells recognizing gp100 and tyrosinase
are present at the vitiligo lesions. These T cells secrete IFN-γ and IL-2 upon peptide specific stimulation as well as upon
recognition of the autologous tumor. We show that functional CD8+ T cells specific for melanoma differentiation antigens used in a melanoma immunotherapy trial, do not only invade the tumor,
but also the vitiligo lesions. This directly links vitiligo to the immuno-therapeutic intervention and supports the hypothesis
that vitiligo is a marker of immunity against melanoma cells.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
83.
Buschow SI Lasonder E Szklarczyk R Oud MM de Vries IJ Figdor CG 《Journal of Proteomics》2012,75(5):1547-1562
Dendritic cells (DC) take up pathogens through phagocytosis and process them into protein and lipid fragments for presentation to T cells. So far, the proteome of the human DC phagosome, a detrimental compartment for antigen processing and presentation as well as for DC activation, remains largely uncharacterized. Here we have analyzed the protein composition of phagosomes from human monocyte-derived DC. For LC-MS/MS analysis we purified phagosomes from DC using latex beads targeted to DC-SIGN, and quantified proteins using a label-free method. We used organellar enrichment ranking (OER) to select proteins with a high potential to be relevant for phagosome function. The method compares phagosome protein abundance with protein abundance in whole DC. Phagosome enrichment indicates specific recruitment to the phagosome rather than co-purification or passive incorporation. Using OER we extracted the most enriched proteins that we further complemented with functionally associated proteins to define a set of 90 phagosomal proteins that included many proteins with established relevance on DC phagosomes as well as high potential novel candidates. We already experimentally confirmed phagosomal recruitment of Galectin-9, which has not been previously associated with phagocytosis, to both bead and pathogen containing phagosomes, suggesting a role for Galectin-9 in DC phagocytosis. 相似文献
84.
Tel J van der Leun AM Figdor CG Torensma R de Vries IJ 《Cancer immunology, immunotherapy : CII》2012,61(8):1279-1288
The plasmacytoid dendritic cell (pDC) constitutes a unique DC subset that links the innate and adaptive arm of the immune system. Whereas the unique capability of pDCs to produce large amounts of type I IFNs in response to pathogen recognition is generally accepted,their antigen-presenting function is often neglected since most studies on antigen presentation are aimed at other DC subsets. Recently, pDCs were demonstrated capable to present antigen leading to protective tumor immunity. In this review, we discuss how pDCs could be exploited in the fight against cancer by analyzing their capacity to capture,process and (cross-) present antigen. 相似文献
85.
Sabine Milde Georg Hemmrich Friederike Anton-Erxleben Konstantin Khalturin Jörg Wittlieb Thomas CG Bosch 《Genome biology》2009,10(1):R8-16
Background
Despite decades of research, the molecular mechanisms responsible for the evolution of morphological diversity remain poorly understood. While current models assume that species-specific morphologies are governed by differential use of conserved genetic regulatory circuits, it is debated whether non-conserved taxonomically restricted genes are also involved in making taxonomically relevant structures. The genomic resources available in Hydra, a member of the early branching animal phylum Cnidaria, provide a unique opportunity to study the molecular evolution of morphological novelties such as the nematocyte, a cell type characteristic of, and unique to, Cnidaria. 相似文献86.
Lindhout E Vissers JL Hartgers FC Huijbens RJ Scharenborg NM Figdor CG Adema GJ 《Journal of immunology (Baltimore, Md. : 1950)》2001,166(5):3284-3289
DC-CK1 (CCL18) is a dendritic cell (DC)-specific chemokine expressed in both T and B cell areas of secondary lymphoid organs that preferentially attracts CD45RA(+) T cells. In this study, we further explored the nature of DC-CK1 expressing cells in germinal centers (GCs) of secondary lymphoid organs using a newly developed anti-DC-CK1 mAb. Immunohistochemical analysis demonstrated a remarkable difference in the number of DC-CK1 expressing cells in adjacent GCs within one tonsil, implicating that the expression of DC-CK1 in GCs depends on the activation and/or progression stage of the GC reaction. Using immunohistology and RNA analysis, we demonstrated that GCDC are the source of DC-CK1 production in the GCs. Considering the recently described function of GCDC in (naive) B cell proliferation, isotype switching and Ab production, we investigated the ability of DC-CK1 to attract B lymphocytes. Here we demonstrate that DC-CK1 is a pertussis toxin-dependent chemoattractant for B lymphocytes with a preference in attracting mantle zone (CD38(-)) B cells. The findings that GCDC produce DC-CK1 and attract mantle zone B cells support a key role for GCDC in the development of GCs and memory B cell formation. 相似文献
87.
Dendritic cell immunotherapy: mapping the way 总被引:30,自引:0,他引:30
Dendritic cells (DCs) are the professional antigen-presenting cells of the immune system, with the potential to either stimulate or inhibit immune responses. Exploiting the immune-regulatory capacities of dendritic cells holds great promise for the treatment of cancer, autoimmune diseases and the prevention of transplant rejection. Although early clinical trials indicate that DC vaccines can induce immune responses in some cancer patients, careful study design and use of standardized clinical and immunological criteria are needed. 相似文献
88.
Single-copy nuclear DNAs (scnDNAs) of eight species of arvicoline and six
species of murine rodents were compared using DNA-DNA hybridization. The
branching pattern derived from the DNA comparisons is congruent with the
fossil evidence and supported by comparative biochemical, chromosomal, and
morphological studies. The recently improved fossil record for these
lineages provides seven approximate divergence dates, which were used to
calibrate the DNA-hybridization data. The average rate of scnDNA divergence
was estimated as 2.5%/Myr. This is approximately 10 times the rate in the
hominoid primates. These results agree with previous reports of accelerated
DNA evolution in muroid rodents and extend the DNA-DNA hybridization data
set of Brownell.
相似文献
89.
Small-subunit ribosomal RNA sequence from Naegleria gruberi supports the polyphyletic origin of amoebas 总被引:2,自引:0,他引:2
We have sequenced the small-subunit ribosomal RNA gene of the amoebo-
flagellate protozoan Naegleria gruberi. Comparison of this sequence with
the rRNA sequences of other eukaryotes resulted in a phylogenetic tree that
supports the suggested polyphyletic origin of amoebas and suggests a
flagellate ancestry for Naegleria.
相似文献
90.
H Spits H Yssel X Paliard R Kastelein C Figdor J E de Vries 《Journal of immunology (Baltimore, Md. : 1950)》1988,141(1):29-36
Human rIL-4 was studied for its capacity to induce lymphokine-activated killer (LAK) cell activity. In contrast to IL-2, IL-4 was not able to induce LAK cell activity in cell cultures derived from peripheral blood. IL-4 added simultaneously with IL-2 to such cultures suppressed IL-2-induced LAK cell activity measured against Daudi and the melanoma cell line MEWO in a dose-dependent way. IL-4 also inhibited the induction of LAK cell activity in CD2+, CD3-, CD4-, CD8- cells, suggesting that IL-4 acts directly on LAK precursor cells. IL-4 added 24 h after the addition of IL-2 failed to inhibit the generation of LAK cell activity. Cytotoxic activity of various types of NK cell clones was not affected after incubation in IL-4 for 3 days, indicating that IL-4 does not affect the activity of already committed killer cells. No significant differences were observed in the percentages of Tac+, NKH-1+ and CD16+ cells after culturing PBL in IL-2, IL-4 or combinations of IL-2 and IL-4 for 3 days. IL-4 also inhibited the activation of non-specific cytotoxic activity in MLC, as measured against K-562 and MEWO cells. In contrast, the Ag-specific CTL activity against the stimulator cells was augmented by IL-4. Collectively, these data indicate that IL-4 prevents the activation of LAK cell precursors by IL-2, but does not inhibit the generation of Ag-specific CTL. 相似文献