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91.
Effects of climate warming and prolonged snow cover on phenology of the early life history stages of four alpine herbs on the southeastern Tibetan Plateau 下载免费PDF全文
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Volkan Okur Megan T. Cho Lindsay Henderson Kyle Retterer Michael Schneider Shannon Sattler Dmitriy Niyazov Meron Azage Sharon Smith Jonathan Picker Sharyn Lincoln Mark Tarnopolsky Lauren Brady Hans T. Bjornsson Carolyn Applegate Amy Dameron Rebecca Willaert Berivan Baskin Jane Juusola Wendy K. Chung 《Human genetics》2016,135(7):699-705
94.
Results of field and glasshouse experiments on Solidago shortii, and our observations on this species over many years, were used to construct a conceptual model of the roles of succession, light, soil nutrients and disturbance on population vigor and maintenance of this federal-endangered species. As cover of woody vegetation increased at a population site between 1986 and 1992, number of flowering ramets of S. shortii significantly decreased but number of vegetative ramets remained nearly constant. Adult plants transplanted into a redcedar thicket and those shaded in a glasshouse produced many fewer flowering ramets and capitula per flowering ramet and less biomass and had higher mortality than those in the open. Seedlings/juveniles shaded in a glasshouse had significantly less dry biomass; lower RGR, NAR, leaf area and root/shoot ratio and higher LAR, SLA and LWR than nonshaded ones. In a field site and glasshouse, fertilized plants (NPK) consistently had more flowering ramets and capitula per flowering ramet than nonfertilized ones. Hierarchy of dry weight of plants grown in a glasshouse in soils derived from five types of bedrock was phosphatic limestone > calcareous shale > sandstone > black shale = dolomite. Flowering and biomass production in the field-fertilizer and soil-type experiments were associated closely with levels of P. Number of flowering ramets significantly increased in plants transferred from shaded to nonshaded glasshouse conditions, but no such increase occurred after opening the canopy above plants in a thicket. Both high light and high nutrient levels apparently are necessary to maintain high vigor of S. shortii. In areas subject to invasion by woody plants, periodic high intensity disturbance may be required to prevent population extirpation. 相似文献
95.
Temperature‐compensated cell production rate and elongation zone length in the root of Arabidopsis thaliana 下载免费PDF全文
Xiaoli Yang Gang Dong K. Palaniappan Guohua Mi Tobias I. Baskin 《Plant, cell & environment》2017,40(2):264-276
To understand how root growth responds to temperature, we used kinematic analysis to quantify division and expansion parameters in the root of Arabidopsis thaliana. Plants were grown at temperatures from 15 to 30 °C, given continuously from germination. Over these temperatures, root length varies more than threefold in the wild type but by only twofold in a double mutant for phytochrome‐interacting factor 4 and 5. For kinematics, the spatial profile of velocity was obtained with new software, Stripflow. We find that 30 °C truncates the elongation zone and curtails cell production, responses that probably reflect the elicitation of a common pathway for handling severe stresses. Curiously, rates of cell division at all temperatures are closely correlated with rates of radial expansion. Between 15 to 25 °C, root growth rate, maximal elemental elongation rate, and final cell length scale positively with temperature whereas the length of the meristem scales negatively. Non‐linear temperature scaling characterizes meristem cell number, time to transit through either meristem or elongation zone, and average cell division rate. Surprisingly, the length of the elongation zone and the total rate of cell production are temperature invariant, constancies that have implications for our understanding of how the underlying cellular processes are integrated. 相似文献
96.
We compared various aspects of the seed biology of eight non-pioneer tree species from a tropical seasonal rain forest in
Xishuangbanna, SW China, that differ in time of dispersal, size and fresh seed moisture content (MC). Seeds were tested for
germination under laboratory conditions after dehydration to different moisture levels and under 3.5, 10 and 30% solar irradiances
in neutral-shade houses. For six species, germination was also compared in forest understory (3.5% light) and center of a
forest gap (32.5% light). Under continuous dehydration over activated silica gel, 100% of seeds of four species had lost the
ability to germinate after 48 h, and those of all species except Castanopsis hystrix (decreased from >90 to 30% germination) had lost the ability to germinate after 120 h. Four species did not differ in final
germination percentages at the three irradiances (i.e. uniform germination). However, final germination percentages of Horsfieldia pandurifolia and Litsea pierrei var. szemaois were significantly lower in 30% than in 10 or 3.5% light, and seeds of Antiaris toxicaria and C. hystrix germinated to higher percentages in 30 and 10% than in 3.5% light. Mean time to germination (MTG) of the eight species (forest
and shade house data combined) ranged from 5–5 days for Pometia
tomentosa to 72–207days for L. pierrei; MTG for four species was ≤21 days. There was no obvious relationship between relative desiccation resistance and either
time of dispersal, MTG or uniformity of germination at the three light levels, or between seed size and MC or MTG. However,
the relationship between seed MC at maturity (25–60% fresh mass basis) and MC at 50% loss of seed viability (12.4–42.5%) was
significant. Seven of the species fit Garwood’s (Ecol Monogr 53:159–181, 1983) rapid-rainy germination syndrome and one, L. pierrei, either her delayed-rainy or intermediate-dry germination syndrome. However, fresh, non-dehydrated seeds of all eight species
germinated in ≤30 days at constant 30°C in light. 相似文献
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99.
Michael CW Chan Renee WY Chan Wendy CL Yu Carol CC Ho WH Chui CK Lo Kit M Yuen Yi Guan John M Nicholls JS Malik Peiris 《Respiratory research》2009,10(1):102
Background
Highly pathogenic avian influenza (HPAI) H5N1 virus is entrenched in poultry in Asia and Africa and continues to infect humans zoonotically causing acute respiratory disease syndrome and death. There is evidence that the virus may sometimes spread beyond respiratory tract to cause disseminated infection. The primary target cell for HPAI H5N1 virus in human lung is the alveolar epithelial cell. Alveolar epithelium and its adjacent lung microvascular endothelium form host barriers to the initiation of infection and dissemination of influenza H5N1 infection in humans. These are polarized cells and the polarity of influenza virus entry and egress as well as the secretion of cytokines and chemokines from the virus infected cells are likely to be central to the pathogenesis of human H5N1 disease.Aim
To study influenza A (H5N1) virus replication and host innate immune responses in polarized primary human alveolar epithelial cells and lung microvascular endothelial cells and its relevance to the pathogenesis of human H5N1 disease.Methods
We use an in vitro model of polarized primary human alveolar epithelial cells and lung microvascular endothelial cells grown in transwell culture inserts to compare infection with influenza A subtype H1N1 and H5N1 viruses via the apical or basolateral surfaces.Results
We demonstrate that both influenza H1N1 and H5N1 viruses efficiently infect alveolar epithelial cells from both apical and basolateral surface of the epithelium but release of newly formed virus is mainly from the apical side of the epithelium. In contrast, influenza H5N1 virus, but not H1N1 virus, efficiently infected polarized microvascular endothelial cells from both apical and basolateral aspects. This provides a mechanistic explanation for how H5N1 virus may infect the lung from systemic circulation. Epidemiological evidence has implicated ingestion of virus-contaminated foods as the source of infection in some instances and our data suggests that viremia, secondary to, for example, gastro-intestinal infection, can potentially lead to infection of the lung. HPAI H5N1 virus was a more potent inducer of cytokines (e.g. IP-10, RANTES, IL-6) in comparison to H1N1 virus in alveolar epithelial cells, and these virus-induced chemokines were secreted onto both the apical and basolateral aspects of the polarized alveolar epithelium.Conclusion
The predilection of viruses for different routes of entry and egress from the infected cell is important in understanding the pathogenesis of influenza H5N1 infection and may help unravel the pathogenesis of human H5N1 disease. 相似文献100.
Rahman A Nakasone A Chhun T Ooura C Biswas KK Uchimiya H Tsurumi S Baskin TI Tanaka A Oono Y 《The Plant journal : for cell and molecular biology》2006,47(5):788-801
2,4-dichlorophenoxyacetic acid (2,4-D), a chemical analogue of indole-3-acetic acid (IAA), is widely used as a growth regulator and exogenous source of auxin. Because 2,4-D evokes physiological and molecular responses similar to those evoked by IAA, it is believed that they share a common response pathway. Here, we show that a mutant, antiauxin resistant1 (aar1), identified in a screen for resistance to the anti-auxin p-chlorophenoxy-isobutyric acid (PCIB), is resistant to 2,4-D, yet nevertheless responds like the wild-type to IAA and 1-napthaleneacetic acid in root elongation and lateral root induction assays. That the aar1 mutation alters 2,4-D responsiveness specifically was confirmed by analysis of GUS expression in the DR5:GUS and HS:AXR3NT-GUS backgrounds, as well as by real-time PCR quantification of IAA11 expression. The two characterized aar1 alleles both harbor multi-gene deletions; however, 2,4-D responsiveness was restored by transformation with one of the genes missing in both alleles, and the 2,4-D-resistant phenotype was reproduced by decreasing the expression of the same gene in the wild-type using an RNAi construct. The gene encodes a small, acidic protein (SMAP1) with unknown function and present in plants, animals and invertebrates but not in fungi or prokaryotes. Taken together, these results suggest that SMAP1 is a regulatory component that mediates responses to 2,4-D, and that responses to 2,4-D and IAA are partially distinct. 相似文献