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61.
CAROLINE V. DUFFIE TRAVIS C. GLENN†‡ F. HERNAN VARGAS§¶ PATRICIA G. PARKER 《Molecular ecology》2009,18(10):2103-2111
We assessed colony- and island-level genetic differentiation for the flightless cormorant ( Phalacrocorax harrisi ), an endangered Galápagos endemic that has one of the most limited geographical distributions of any seabird, consisting of only two adjacent islands. We screened 223 individuals from both islands and nine colonies at five microsatellite loci, recovering 23 alleles. We found highly significant genetic differentiation throughout the flightless cormorant's range on Fernandina and Isabela Islands (global F ST = 0.097; P < 0.0003) both between islands (supported by Bayesian analyses, F ST and R ST values) and within islands (supported only by F ST and R ST values). An overall pattern of isolation-by-distance was evident throughout the sampled range ( r = 0.4169, one-sided P ≤ 0.02) and partial Mantel tests of this relationship confirmed that ocean is a dispersal barrier ( r = 0.500, one-sided P ≤ 0.003), especially across the 5-km gap between the two islands. The degree of detected genetic differentiation among colonies is surprising, given the flightless cormorant's limited range, and suggests a role for low vagility, behavioural philopatry, or both to limit dispersal where physical barriers are absent. We argue that this population should be managed as at least two genetic populations to better preserve the species-level genetic diversity, but, for demographic reasons, advocate the continued conservation of all breeding colonies. 相似文献
62.
63.
A Comparison of Methods for Measuring Turgor Pressures and Osmotic Pressures of Cells of Red Beet Storage Tissue 总被引:6,自引:0,他引:6
TOMOS A. DERI; LEIGH ROGER A.; SHAW CAROLINE A.; JONES R. GARETH WYN 《Journal of experimental botany》1984,35(11):1675-1683
Two methods for measuring the turgor pressures of cells in discsof storage tissue of red beet (Beta vulgaris L.) were compared,and a centrifugation method for extracting sap from frozen andthawed tissue was evaluated. Turgor pressures were measureddirectly using a pressure probe, or indirectly using a vapourpressure osmometer. With the latter, discs were placed directlyin the osmometer chamber and turgor was calculated as the differencein osmotic pressure before and after freezing and thawing. Turgorin freshly cut discs, measured with the pressure probe, wasbetween 0-012 MPa and 0.118 MPa with a mean ±s.d. of0.092±;0.032 MPa (n = 24). That measured with the osmometervaried between 0.08 MPa and 0.12 MPa with a mean ±s.d.of 0.09±0.10 MPa (n = 54). After vacuum infiltrationof discs with distilled water, the turgor measured with thepressure probe increased to 1.051.12 MPa. Turgor measuredwith the osmometer also increased after vacuum infiltrationbut was, on average, 12% lower than that measured with the pressureprobe. Overall, the results suggest that for routine measurements,the osmometer can provide reasonable estimates of the turgorof cells in beet discs. This is because a number of factorsthat, potentially, could interfere with this method have onlya small effect in this tissue. None of the measured turgorsis indicative of that occurring in intact storage roots becauseboth excision and vacuum infiltration of discs alter the concentrationsof solutes in the extracellular space. The osmotic pressureof sap extracted by centrifugation from frozen and thawed discswas not significantly different from that measured by placingfrozen and thawed discs directly in the osmometer. Solute concentrationsin the sap were not significantly different from those measuredby chemical extraction of discs. Key words: Beta vulgaris, Osmotic pressure, Turgor pressure 相似文献
64.
I. CAROLINE LE POOLE REN M.J.G.J. VAN DEN WIJNGAARD RONALD P. VERKRUISEN WOUT H. LAMERS DIRK TROOST WIETE WESTERHOF PRANAB K. DAS 《Pigment cell & melanoma research》1996,9(3):126-133
In vivo, melanocytes were detected in epidermis from human tissue of 6.5 weeks estimated gestinational age (EGA) and older. We have successfully established melanocyte monocultures from tissue of 9 to 10 weeks EGA. To our knowledge, this is the first report on physiology of human foetal melanocytes in monoculture. In culture, such melanocytes retained foetal characteristics. Proliferation rates noted were markedly higher (approximately 2.7-fold) when compared to those in cultures of neonatal melanocytes. Moreover, when analyzing cellular phenotypes by markers for cells of the melanocytic lineage, foetal cells isolated from tissue of 9 weeks EGA reproducibly showed expression of the high molecular weight (HMW) antigen and c-kit to an extent intermediate to that found in neonatal melanocytes and M14 melanoma cells. Such differential expression was not observed if cells were isolated from tissue of 10 weeks EGA, indicating that the foetal environment provides essential differentiation stimuli during the 10th week of gestation. Moreover, these results are supportive of the theory that malignant transformation involves a process of dedifferentiation. In all, human foetal melanocyte culture provides a useful model to investigate pigment cell differentiation. 相似文献
65.
66.
Host plant effects on the performance of the aphid Aulacorthum solani (Kalt.) (Homoptera: Aphididae) at ambient and elevated CO2 总被引:1,自引:0,他引:1
In future elevated CO2 environments, chewing insects are likely to perform less well than at present because of the effects of increased carbon fixation on their host plants. When the aphid, Aulacorthum solani was reared on bean ( Vicia faba ) and tansy ( Tanacetum vulgare ) plants under ambient and elevated CO2, performance was enhanced on both hosts at elevated CO2 . The nature of the response was different on each plant species suggesting that feeding strategy may influence an insect's response to elevated CO2 . On bean, the daily rate of production of nymphs was increased by 16% but there was no difference in development time, whereas on tansy, development time was 10% shorter at elevated CO2 but the rate of production of nymphs was not affected. The same aphid clone therefore responded differently to elevated CO2 on different host plants. This increase in aphid performance could lead to larger populations of aphids in a future elevated CO2 environment. 相似文献
67.
MOULIA BRUNO; LOUP CAROLINE; CHARTIER MICHEL; ALLIRAND JEAN MICHEL; EDELIN CLAUDE 《Annals of botany》1999,84(5):645-656
The maximal architectural development of a modern maize cultivar(Zea mays L. Dea) was studied in fully isolatedand carefully irrigated plants. Under these favourable conditions,this (usually) non-tillering and non-prolific cultivar displayeda large amount of branching (down to the third order), includinglarge basal tillers and prolific ear shoots. This developmentwas analysed by combining: (1) architectural analysis, initiallydeveloped for trees; and (2) quantitative analysis of tilleringkinetics, designed for other grass species. The architecturalunit of maize included a main long axis (A1) and lateral shortshoots bearing a terminal spike (A2). The basal tillers (notedA1') resulted from a complete and sylleptic reiteration. Itskinetics were consistent with tillering models, but with a particularlylong delay in branch emergence (seven phyllochrons), comparedto other grass species. This delay is likely to enhance regulationby leaf (or root) density in stands and explains the inhibitionof branch development, as usually observed in field conditions,even at low density. Similarly, the suppression of the basalreiteration in secondary branches observed in isolated plantsprobably results from increased intra-tussock density. In isolatedplants, androgenous axes combining A1' and A2 morphologies werealso produced in intermediate positions. It is shown that theycan result from a basipetal sequence of A2 differentiation reachingbuds in the course of their A1' development. The consequencesof these unexpected results are discussed in terms of maizedevelopment and architectural analysis of grasses. Copyright1999 Annals of Botany Company Plant architecture, development, maize (Zea mays L.), tillering, branching, shoot, prolificacy, phyllochron. 相似文献
68.
NILS CORDES FREDERIK ALBRECHT LEIF ENGQVIST TIM SCHMOLL MARKUS BAIER CAROLINE MÜLLER KLAUS REINHOLD 《Ecological Entomology》2015,40(1):34-41
1. Variation in larval food composition can have far‐reaching effects on the adult phenotype of insects. To maximise reproductive output, it is therefore beneficial if insects are able to plastically adapt to nutritional cues in their larval diet. 2. The expression of sexual traits implicated in pre‐ and postcopulatory sexual selection of the lesser wax moth, Achroia grisella Fabricius, across different rearing environments of varying diet composition was investigated. 3. Moths reared on diet with relatively low amounts of carbohydrate, but more protein and fats, had lower survival rates, decreased body mass, and longer development times. Males reared on this diet produced songs with higher pulse pair rates (which are attractive to females) and transferred more sperm per copulation than males reared on the alternative diets. 4. There was no evidence for a trade‐off between pre‐ and postcopulatory traits. Interestingly, individuals with both higher pulse pair rates and more transferred sperm came from the treatment group with higher mortality rates and generally poorer development. The present results suggest that both of these sexual characteristics are developmentally plastic, but that only moths reared on the protein‐rich diet were able to benefit from this plasticity. 相似文献
69.
CAROLINE NORTHWOOD† 《Palaeontology》2005,48(1):49-68
Abstract: Coprolites from the Arcadia Formation, Queensland, Australia, were studied in conjunction with the vertebrate fossil assemblages from two localities to maximize our understanding of the palaeoecology of these Early Triassic deposits. Criteria used by other researchers to identify the producers of coprolites were found to be of little value in the Arcadia Formation specimens. Using a combination of shape, biostratigraphic distribution, size and included remains some of the coprolites are attributed to basal archosauromorphs and fish whereas others could not be identified. Perhaps the most important attribute of the Arcadia coprolites is that they preserved rare organisms such as cyanobacteria, insects and other arthropods, and a diversity of fish. Estimates of the number of actinopterygians and dipnoans preserved in coprolites significantly increased relative abundance estimates based on skeletal elements alone. Although coprolites are an important source of palaeobiological information, this information is limited by our poor understanding of the taphonomic processes involved in the fossilization of faecal matter and by the near impossibility of assigning coprolites to specific producers. 相似文献
70.
Thresholds and responses to capsaicin in oil- and water-based model systems were compared. Thresholds were measured among 23 individuals using an ascending forced choice method of limits. Detection thresholds were 11.75 (± 1.31) mg/L in oil and 0.31 (± 0.03) mg/L in water. A horizontal labeled magnitude scale was used to obtain suprathreshold scaling functions in corn and soybean oils and in water systems using an emulsifier (polysorbate) or alcohol to solvate the capsaicin. In the water-based systems, capsaicin ranged from weak to very strong ratings over a range of 0.3 to 10 mg/L while the same perceptual range was achieved in oil over a range of 10 to 316 mg/L. Nonconsumers of hot/spicy foods generally had higher thresholds and higher suprathreshold responses in oil systems. Differences between user groups were less pronounced in water-based stimuli. 相似文献