全文获取类型
收费全文 | 183篇 |
免费 | 3篇 |
专业分类
186篇 |
出版年
2015年 | 2篇 |
2013年 | 3篇 |
2012年 | 3篇 |
2011年 | 3篇 |
2010年 | 4篇 |
2009年 | 9篇 |
2008年 | 6篇 |
2007年 | 8篇 |
2006年 | 4篇 |
2005年 | 10篇 |
2004年 | 5篇 |
2003年 | 3篇 |
2001年 | 5篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 5篇 |
1994年 | 3篇 |
1993年 | 6篇 |
1992年 | 7篇 |
1991年 | 9篇 |
1990年 | 7篇 |
1989年 | 5篇 |
1988年 | 3篇 |
1987年 | 6篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1972年 | 1篇 |
1971年 | 5篇 |
1970年 | 3篇 |
1969年 | 4篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1965年 | 1篇 |
1957年 | 1篇 |
1953年 | 1篇 |
1950年 | 2篇 |
排序方式: 共有186条查询结果,搜索用时 10 毫秒
11.
JAMAL Z. FAROOQUI BILLYE W. AUCLAIR EDWARD ROBB EDWARD SARKISIAN CAROL COOPER J. WESLEY ALEXANDER GLEN WARDEN RAYMOND E. BOISSY JAMES NORLUND 《Pigment cell & melanoma research》1993,6(4):226-233
The mechanisms for hyperpigmentation observed in human cutaneous xenografts placed on athymic nude mice was investigated. Histologic, biochemical, histochemical, and ultrastructural examinations were performed on human skin prior to grafting and at various times ranging from 2 weeks to 30 weeks post-grafting (PG). Hyperpigmentation was macroscopically visible on the graft as early as 4–6 weeks. The number of Dopa-positive melanocytes per unit area was increased at 2 weeks PG and remained elevated until 20 weeks PG. The surface area of the melanocytes, a measure of the activity of the cells, also increased significantly and remained above the pre-grafting size throughout the study. Western blot analysis using tyrosinase specific antibody (αTy-SP) revealed the presence of tyrosinase exclusively in the grafted skin from 2 weeks to 12 weeks PG tested. Histological and ultrastructural observations revealed the presence of numerous dendritic melanocytes, indeterminant clear cells suggestive of Langerhans cells, and dermal melanophages. The results of this study suggest that the observed hyperpigmentation in grafted tissue is caused by an increase in the number of Dopa-positive melanocytes and probably from enhanced melanin production. Extracts of proteins from the xenografts exhibited prominent differences in low and high molecular proteins between pre- and post-grafted skin. Among them, the exclusive appearance of a protein doublet with apparent mw ~14 kDa was found in grafted skin, and subsequent studies indicate it has potent effects on melanocyte function. 相似文献
12.
13.
14.
Signalling self‐ability to maintain vigilance may help in securing a mate, while providing accurate information about vigilance status may result in conspecifics adjusting their own scanning rate of the environment, potentially to the individual's benefit. In birds, vigilance is often associated with head‐up postures adopted within a bout of head‐down activity, and this can be used by conspecifics to assess the vigilance of their flock mates. However, vigilance behaviour is not always obvious and other cues may then be used to assess vigilance rates of conspecifics. Here we assess whether iris/eyelid/face patterns from 43 duck species are consistent with the hypothesis that eyelid brightness has evolved so as to contrast with iris brightness, which may then help in signalling individual vigilance status. Ducks generally flock when resting during the day, and because of their wide visual fields, individuals can monitor their environment while remaining in a resting head‐down position. Ducks also show a wide variety of plumage and iris patterns, with both light‐headed and dark‐headed species. Matching our prediction, most ducks with dark irises had pale eyelids, irrespective of head colour. Furthermore, the smaller number of species with a pale iris generally have darker eyelids. A phylogenetic analysis shows a clear and significant association in the evolution of eyelid and iris brightness patterns in both males and females. These data therefore provide support for the hypothesis that eyelid brightness has evolved to act as a contrast with iris brightness. Further studies are now needed to examine the extent to which and the way this is used in vigilance information transfer between individuals. 相似文献
15.
DAVID BODIE CAROL A. BENNETT-CLARKE KATHLEEN DAVIS JENNIFER P. POSTELWAITE NICOLAS L. CHIAIA ROBERT W. RHOADES 《Somatosensory & motor research》2013,30(3):168-180
Previous experiments from this laboratory have indicated that transection of the infraorbital nerve (ION, the trigeminal \[V] branch that supplies the mystacial vibrissae follicles) at birth and in adulthood has markedly different effects on galanin immunoreactivity in the V brainstem complex. Adult nerve transection increases galanin immunoreactivity in the superficial layers of V subnucleus caudalis (SpC) only, while neonatal nerve transection results in increased galanin expression in vibrissae-related primary afferents throughout the V brainstem complex. The present study describes the distribution of binding sites for this peptide in the mature and developing V ganglion and brainstem complex and determines the effects of neonatal and adult ION damage and the associated changes in galanin levels upon their distribution and density. Galanin binding sites are densely distributed in all V brainstem subnuclei and are particularly dense in V subnucleus interpolaris and the superficial layers of SpC. They are present at birth (P-0) and their distribution is similar to that in adult animals. Transection of the ION in adulthood and examination of brainstem 7 days later indicated marked reductions in the density of galanin binding sites in the V brainstem complex. With the exception of the superficial laminae of SpC, the same reduction in density remained apparent in rats that survived 45 days after nerve cuts. Transection of the ION on P-0 resulted in no change in the density of galanin binding sites in the brainstem after either 7 or 60 days survival. These results indicate that densely distributed galanin binding sites are present in the V brainstem complex of both neonatal and adult rats, that they are located in regions not innervated by galanin-positive axons, and that their density is not significantly influenced by large lesion-induced changes in the primary afferent content of their natural ligand. 相似文献
16.
Mitochondrial (mt) DNA control region sequences were used to test the genetic and phylogeographic structure of walleye Stizostedion vitreum populations at different geographical scales: among spawning sites, lake basins, lakes, and putative glacial refugia in the Great Lakes region. Sequencing 199 walleye revealed nucleotide substitutions and tandemly repeated sequences that varied in copy number, as well as in sequence composition, in 1200 bp of the mtDNA control region. Variable numbers of copies of an 11-bp tandem repeat showed no geographical patterning and were not used in further analyses. Substitutions in the other areas of the control region yielded 19 haplotypes, revealing phylogeographic structure and significant differences among glacial refugia, lakes, basins and some spawning sites. Differences among spawning populations were consistent with reduced gene flow, philopatry and possible natal homing. Analysis of spawning populations showed consistency of genotypic frequencies among years and between males and females, supporting philopatry in both sexes. The unglaciated plateau in southern Ohio, USA housed a very different haplotype that diverged prior to the Missouri, Mississippi and Atlantic glacial refugia types. Haplotypes from the three refugia colonized the Great Lakes after retreat of the Wisconsin glaciers, and their present distribution reflects the geography of their prior isolation and differential colonization. Populations that became associated with spawning localities appear to have diverged further due to philopatry, resulting in fine-scale phylogeographic structuring. 相似文献
17.
18.
CAROL A. FURNESS fls 《Botanical journal of the Linnean Society. Linnean Society of London》2007,155(1):29-48
Apertures are key characters of pollen grains with systematic importance in angiosperms. They function as sites for pollen tube exit, water uptake, transfer of recognition substances and accommodation of volume changes. Not all pollen has apertures; inaperturate pollen (lacking obvious apertures) characterizes many angiosperm groups, especially in early divergent angiosperms and monocots, but also eudicots. In order to expand our knowledge of the systematic distribution, possible functional significance and development of inaperturate pollen in angiosperms, this review focuses on inaperturate and cryptoaperturate (with hidden apertures) pollen in the large eudicot clade, which comprises about 75% of present‐day angiosperm species. It includes new TEM observations of inaperturate pollen from four exemplar taxa selected from different parts of the eudicot phylogeny. Two categories of inaperturate (including cryptoaperturate) pollen occur in eudicots. (1) Sterile attractant or feeding pollen associated with functional dioecy has evolved iteratively at least six times in conjunction with complex breeding systems in the core eudicots. (2) Fertile pollen has evolved numerous times independently throughout eudicots, though generally in a relatively small number of individual taxa. Notable exceptions are the petaliferous crotonoid Euphorbiaceae s.s., in which fertile inaperturate pollen occurs in c. 1500 species, and two subfamilies of Apocynaceae s.l. (Secamonoideae and Asclepiadoideae) with c. 2500 species with fertile inaperturate pollen in pollinia. Fertile inaperturate pollen is sometimes (but not always) associated with an aquatic habit, parasitism, insectivory, heterostyly, anemophily or pollinia. Most fertile inaperturate pollen has a thin exine, or the exine is largely restricted to isolated components (muri, protuberances, subunits) separated by thinner areas which probably function as apertures. In cryptoaperturate pollen, the aperture is covered by continuous exine which probably has a protective function, similar to an operculum. Developmentally, inaperturate pollen is not associated with any particular tetrad type or meiotic spindle orientation (unlike some apertures) due to the absence of a colpal shield of endoplasmic reticulum or other organelles and hence is independent of microsporogenesis type. The lack of a colpal shield during the tetrad stage of development permits complete deposition of first primexine and then exine around each microspore, possibly mediated by the action of the DEX1 protein. © 2007 The Linnean Society of London, Botanical Journal of the Linnean Society, 2007, 155 , 29–48. 相似文献
19.
20.
Response of grazing snails to phosphorus enrichment of modern stromatolitic microbial communities 总被引:1,自引:0,他引:1
JAMES J. ELSER JOHN H. SCHAMPEL MARCIA KYLE JAMES WATTS EVAN W. CARSON THOMAS E. DOWLING CAROL TANG PETER D. ROOPNARINE 《Freshwater Biology》2005,50(11):1826-1835
1. The effects of added phosphorus (P) on the growth, P and RNA : DNA contents, and survivorship of snails grazing on laminated microbial mats (living ‘stromatolites’) were examined in the Rio Mesquites at Cuatro Ciénegas, Mexico (total P, c. 0.60 μmol L?1) to test the hypothesis that strong P‐limitation of microautotroph growth produces a stoichiometric constraint on herbivores because of mineral P‐limitation. 2. In a 3‐week experiment performed in summer 2001, addition of phosphorus (+15 μmol L?1) resulted in a strong decline in stromatolite biomass C : P ratio from very high levels (c. 2300 : 1 by atoms) to moderate levels (c. 550 : 1). The endemic hydrobiid snail Mexithauma quadripaludium responded to P‐enrichment with elevated body P content and higher RNA : DNA ratios, especially for small animals likely to be actively growing. This positive response is consistent with the existence of a stoichiometric constraint on snail growth. 3. In a longer experiment (8 weeks) involving a more moderate P enrichment (+5 μmol L?1) in summer 2002, P enrichment reduced stromatolite C : P ratio from moderate values in control treatments (c. 750) to very low values (<100 : 1). Snails responded to stromatolite P‐enrichment with increased body P content but, in contrast to the first experiment, with lower RNA : DNA ratio, lower growth rates, and higher mortality. 4. These contrasting results suggest that both very high and very low biomass C : P ratios in stromatolites are detrimental to M. quadripaludium performance, leading us to hypothesise that these herbivores live on a ‘stoichiometric knife edge’. 相似文献