全文获取类型
收费全文 | 771700篇 |
免费 | 90216篇 |
国内免费 | 422篇 |
专业分类
862338篇 |
出版年
2018年 | 6623篇 |
2017年 | 6342篇 |
2016年 | 9236篇 |
2015年 | 13058篇 |
2014年 | 14923篇 |
2013年 | 21571篇 |
2012年 | 24407篇 |
2011年 | 24651篇 |
2010年 | 16511篇 |
2009年 | 15248篇 |
2008年 | 21984篇 |
2007年 | 22614篇 |
2006年 | 20945篇 |
2005年 | 20239篇 |
2004年 | 19923篇 |
2003年 | 19189篇 |
2002年 | 18580篇 |
2001年 | 34929篇 |
2000年 | 35315篇 |
1999年 | 28483篇 |
1998年 | 10329篇 |
1997年 | 10746篇 |
1996年 | 10366篇 |
1995年 | 9487篇 |
1994年 | 9661篇 |
1993年 | 9392篇 |
1992年 | 23357篇 |
1991年 | 22323篇 |
1990年 | 22010篇 |
1989年 | 21915篇 |
1988年 | 19767篇 |
1987年 | 18950篇 |
1986年 | 17535篇 |
1985年 | 17432篇 |
1984年 | 14543篇 |
1983年 | 12708篇 |
1982年 | 9834篇 |
1981年 | 8808篇 |
1980年 | 8341篇 |
1979年 | 13870篇 |
1978年 | 10773篇 |
1977年 | 9755篇 |
1976年 | 9339篇 |
1975年 | 10043篇 |
1974年 | 10642篇 |
1973年 | 10439篇 |
1972年 | 9521篇 |
1971年 | 8632篇 |
1970年 | 7424篇 |
1969年 | 7047篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
81.
I. N. Semenchuk L. A. Taranova A. A. Kalenyuk P. V. Il’yasov A. N. Reshetilov 《Applied Biochemistry and Microbiology》2000,36(1):69-72
The operating and storage stability of a receptor element of an amperometric biosensor based on thePseudomonas rathonis strain T capable of degrading surfactants was tested. Microbial cells were immobilized by incorporation in gels (agar, agarose,
and calcium-alginate), polyvinyl alcohol membrane, adhesion to Chromatographic paper GF/A, or by cross-linking induced by
glutaric aldehyde. Incorporation of microbial cells in agar gel provides long-standing conservation of their activity and
viability during measurements of high concentrations of surfactants and allows the receptor element of the biosensor to be
rapidly recovered after measurements. 相似文献
82.
Grape thaumatin-like proteins (TLPs) play roles in plant-pathogen interactions and can cause protein haze in white wine unless removed prior to bottling. Different isoforms of TLPs have different hazing potential and aggregation behavior. Here we present the elucidation of the molecular structures of three grape TLPs that display different hazing potential. The three TLPs have very similar structures despite belonging to two different classes (F2/4JRU is a thaumatin-like protein while I/4L5H and H2/4MBT are VVTL1), and having different unfolding temperatures (56 vs. 62°C), with protein F2/4JRU being heat unstable and forming haze, while I/4L5H does not. These differences in properties are attributable to the conformation of a single loop and the amino acid composition of its flanking regions. 相似文献
83.
Tomohiro Hamasaki Takahiro Matsumoto Naoya Sakamoto Akiko Shimahara Shiori Kato Ayumi Yoshitake Ayumi Utsunomiya Hisayoshi Yurimoto Esteban C. Gabazza Tadaaki Ohgi 《Nucleic acids research》2013,41(12):e126
Radioisotopes and fluorescent compounds are frequently used for RNA labeling but are unsuitable for clinical studies of RNA drugs because of the risk from radiation exposure or the nonequivalence arising from covalently attached fluorophores. Here, we report a practical phosphoramidite solid-phase synthesis of 18O-labeled RNA that avoids these disadvantages, and we demonstrate its application to quantification and imaging. The synthesis involves the introduction of a nonbridging 18O atom into the phosphate group during the oxidation step of the synthetic cycle by using 18O water as the oxygen donor. The 18O label in the RNA was stable at pH 3–8.5, while the physicochemical and biological properties of labeled and unlabeled short interfering RNA were indistinguishable by circular dichroism, melting temperature and RNA-interference activity. The 18O/16O ratio as measured by isotope ratio mass spectrometry increased linearly with the concentration of 18O-labeled RNA, and this technique was used to determine the blood concentration of 18O-labeled RNA after administration to mice. 18O-labeled RNA transfected into human A549 cells was visualized by isotope microscopy. The RNA was observed in foci in the cytoplasm around the nucleus, presumably corresponding to endosomes. These methodologies may be useful for kinetic and cellular-localization studies of RNA in basic and pharmaceutical studies. 相似文献
84.
85.
D S Faffe G H Silva P M Kurtz E M Negri V L Capelozzi P R Rocco W A Zin 《Journal of applied physiology》2001,90(4):1400-1406
The dynamic mechanical properties of lung tissue and its contents of collagen and elastic fibers were studied in strips prepared from mice instilled intratracheally with saline (C) or silica [15 (S15) and 30 days (S30) after instillation]. Resistance, elastance, and hysteresivity were studied during oscillations at different frequencies on S15 and S30. Elastance increased from C to silica groups but was similar between S15 and S30. Resistance was augmented from C to S15 and S30 and was greater in S30 than in S15 at higher frequencies. Hysteresivity was higher in S30 than in C and S15. Silica groups presented a greater amount of collagen than did C. Elastic fiber content increased progressively along time. This increment was related to the higher amount of oxytalan fibers at 15 and 30 days, whereas elaunin and fully developed elastic fibers were augmented only at 30 days. Silicosis led not only to pulmonary fibrosis but also to fibroelastosis, thus assigning a major role to the elastic system in the silicotic lung. 相似文献
86.
Disseminated nocardiosis in three macaque monkeys 总被引:1,自引:0,他引:1
Extrapulmonary nocardiosis was diagnosed at necropsy in two rhesus monkeys (Macaca mulatta) and one pigtailed monkey (M. nemestrina) over a four-year period in a large primate center. Typical lesions were multiple pyogranulomatous foci in the liver, intestines, peritoneum, lung and brain. Partially acid-fast, branching, filamentous organisms were seen in all lesions. Nocardia sp. was isolated from two cases. We postulate that two of the monkeys were infected by the oral route because of the distribution of lesions. 相似文献
87.
88.
S-adenosyl-L-homocysteine (S-AH), a potent inhibitor of biological transmethylation, decreased the response of rat retina adenylate cyclase to dopamine and to 2-amino-6, 7-dihydroxytetrahydronaphtalene (ADTN). This effect appeared for 10?7M of S-adenosyl-L-homocysteine and was linear for concentration ranging to 10?4M. S-adenosyl-L-homocysteine did not decrease the cyclic AMP accumulation with sodium fluoride, a non specific adenylate cyclase activator. On the other hand, the incorporation of methyl group was reduced in rat retina homogenates by S-adenosyl-L-homocysteine. These findings suggest that the activity of the dopamine dependent adenylate cyclase is linked to a methylation process. 相似文献
89.
E. N. Medvedeva N. A. Neverova T. E. Fedorova V. A. Babkin E. S. Meteleva A. V. Dushkin T. G. Tolstikova M. V. Khvostov M. P. Dolgikh 《Russian Journal of Bioorganic Chemistry》2010,36(7):853-859
It has been found by gel-penetrating chromatography and quantitative 13C NMR spectroscopy that mechanochemical processing of arabinogalactan isolated from the wood of the Siberian larch changes
the molecular mass distribution, monosaccharide composition, and the degree of branching of its macromolecules. This is due
to the partial destruction of polysaccharide macromolecules and subsequent recombination of resulting fragments. The intensity
of these processes depends on the conditions of mechanochemical processing. Based on the data of IR and 13C NMR spectra, the conclusion has been drawn that no functionalization of arabinogalactan macromolecules occurs under these
conditions. The toxicopharmacological properties of mechanically processed arabinogalactan have been studied. Its acute toxicity
is >5000 mg/kg. 相似文献
90.