全文获取类型
收费全文 | 654102篇 |
免费 | 72819篇 |
国内免费 | 522篇 |
出版年
2018年 | 6060篇 |
2017年 | 5737篇 |
2016年 | 8362篇 |
2015年 | 11072篇 |
2014年 | 12909篇 |
2013年 | 18348篇 |
2012年 | 20761篇 |
2011年 | 21486篇 |
2010年 | 14443篇 |
2009年 | 13138篇 |
2008年 | 18820篇 |
2007年 | 19470篇 |
2006年 | 17908篇 |
2005年 | 17452篇 |
2004年 | 17191篇 |
2003年 | 16480篇 |
2002年 | 15868篇 |
2001年 | 30838篇 |
2000年 | 30946篇 |
1999年 | 24424篇 |
1998年 | 8286篇 |
1997年 | 8631篇 |
1996年 | 8362篇 |
1995年 | 7574篇 |
1994年 | 7639篇 |
1993年 | 7375篇 |
1992年 | 19424篇 |
1991年 | 18683篇 |
1990年 | 18459篇 |
1989年 | 18117篇 |
1988年 | 16401篇 |
1987年 | 15794篇 |
1986年 | 14408篇 |
1985年 | 14479篇 |
1984年 | 11921篇 |
1983年 | 10325篇 |
1982年 | 7765篇 |
1981年 | 7147篇 |
1980年 | 6601篇 |
1979年 | 11157篇 |
1978年 | 8656篇 |
1977年 | 7947篇 |
1976年 | 7551篇 |
1975年 | 8270篇 |
1974年 | 8837篇 |
1973年 | 8774篇 |
1972年 | 7806篇 |
1971年 | 7246篇 |
1970年 | 6209篇 |
1969年 | 5902篇 |
排序方式: 共有10000条查询结果,搜索用时 484 毫秒
951.
952.
953.
954.
The binding of norepinephrine (NE) to plasma proteins of fresh human blood obtained from healthy volunteers was studied by ultrafiltration at different NE concentrations and incubation times at 37 degrees C. At 1.7 nM L-[3H]-NE binding was approximately 25%. The binding was rapid and was not influenced by the incubation time. [3H]-NE could be dissociated from its binding sites by acid precipitation and, after HPLC, showed to be unchanged NE. No difference in NE binding was found between plasma collected in EGTA-GSH or heparin solution. There was no degradation of NE when incubated in plasma at 37 degrees C for 10 h, even without the addition of antioxidants. Therefore, in the present study, binding represented interaction of unchanged NE with plasma proteins. The whole plasma binding was saturable over the range of 0.66 nM to 0.59 mM of NE. Scatchard plot of specific binding revealed high-affinity sites with a Kd of 5.4 nM and a Bmax of 3.9 fmoles.mg-1 protein, and low-affinity sites with a Kd of 2.7 microM and a Bmax of 3.3 pmoles.mg-1 protein. Electrophoretic characterization of NE-binding proteins showed that about 60% of bound NE was associated to albumin, and 20% to prealbumin. NE binding to pure human plasma proteins was also studied using ultrafiltration. Scatchard analyses revealed a single class of very high-affinity binding sites for prealbumin (Kd 4.9 nM), a single class of binding sites for alpha 1-acid glycoprotein (Kd 54 microM) and two classes of binding sites for albumin with high (Kd 1.7 microM) and low (Kd 0.8 mM) affinities respectively. The main results obtained in this study - a) reversibility of NE binding, b) stability of free and bound NE in plasma, c) involvement of the prealbumin as a specific binding protein - point out to a specific transport for NE in human blood plasma. 相似文献
955.
Using comparative ion-exchange chromatography on Dowex 1X4, the product of dephosphorylation of fructose 2,6-bisphosphate with purified yeast fructose-2,6-bisphosphate 6-phosphohydrolase, was shown to be identical to the furanose form of fructose 2-phosphate prepared by chemical synthesis according to Pontis and Fischer [Biochem. J. 89, 452-459 (1963)]. As expected for the furanose form of fructose 2-phosphate, the enzymatically formed product consumes 1 mol periodate/mol fructose 2-phosphate, whereas the chemically synthesized pyranose form consumes 2 mol periodate/mol. In addition, it is shown that the enzymatic product behaves identically to the furanose, not the pyranose, form of fructose 2-phosphate in hydrolysis of the ester bond at pH 4 and 37 degrees C, as described previously for the chemically synthesized compounds [Pontis and Fischer (1963) vide supra]. 相似文献
956.
957.
958.
959.
O Chisaka K Araki T Ochiya T Tsurimoto W Hiranyawasitte-Attatippaholkun N Yanaihara K Matsubara 《Gene》1987,60(2-3):183-189
A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles. 相似文献
960.
Charles Romeo Naoko Moriwaki Kerry T. Yasunobu Irwin C. Gunsalus Hideo Koga 《Journal of Protein Chemistry》1987,6(3):253-261
The first 12 NH2-terminal amino acids of the Pseudomonas putida putidaredoxin reductase were shown to be Met-Asn-Ala-Asn-Asp-Asn-Val-Val-Ile-Val-Gly-Thr. Comparison of these data with the DNA sequence of the BamHI-HindIII 197-base fragment derived from the PstI 2.2-kb fragment obtained from the P. putida plasmid showed that the putidaredoxin reductase gene was downstream from the cytochrome P-450 gene and the intergenic region had the 24-nucleotide sequence TAAACACATGGGAGTGCGTGCTAA. The Shine-Dalgarno sequence GGAG was detected in this region. The initiating triplet for the reductase gene was GTG, which normally codes for valine, but in the initiating codon position codes for methionine. From the amino acid sequence and X-ray data comparisons with other flavoproteins, what appears to be the AMP binding region of the FAD can be recognized in the NH2-terminal portion of the reductase involving residues 5–35.This article was presented during the proceedings of the International Conference on Macromolecular Structure and Function, held at the National Defence Medical College, Tokorozawa, Japan, December 1985. 相似文献