全文获取类型
收费全文 | 1362篇 |
免费 | 147篇 |
出版年
2022年 | 13篇 |
2021年 | 20篇 |
2020年 | 16篇 |
2019年 | 31篇 |
2018年 | 24篇 |
2017年 | 35篇 |
2016年 | 45篇 |
2015年 | 45篇 |
2014年 | 68篇 |
2013年 | 65篇 |
2012年 | 72篇 |
2011年 | 71篇 |
2010年 | 53篇 |
2009年 | 58篇 |
2008年 | 58篇 |
2007年 | 68篇 |
2006年 | 51篇 |
2005年 | 44篇 |
2004年 | 45篇 |
2003年 | 47篇 |
2002年 | 50篇 |
2001年 | 42篇 |
2000年 | 48篇 |
1999年 | 34篇 |
1998年 | 18篇 |
1997年 | 21篇 |
1996年 | 22篇 |
1995年 | 18篇 |
1994年 | 16篇 |
1993年 | 18篇 |
1992年 | 23篇 |
1991年 | 20篇 |
1990年 | 13篇 |
1989年 | 22篇 |
1988年 | 26篇 |
1987年 | 12篇 |
1986年 | 11篇 |
1985年 | 15篇 |
1984年 | 13篇 |
1983年 | 15篇 |
1982年 | 10篇 |
1979年 | 11篇 |
1978年 | 7篇 |
1977年 | 7篇 |
1975年 | 9篇 |
1973年 | 6篇 |
1971年 | 8篇 |
1970年 | 5篇 |
1967年 | 5篇 |
1965年 | 4篇 |
排序方式: 共有1509条查询结果,搜索用时 15 毫秒
161.
Networks in leaf development 总被引:6,自引:0,他引:6
Byrne ME 《Current opinion in plant biology》2005,8(1):59-66
Shoots are characterized by indeterminate growth resulting from divisions of undifferentiated cells in the central region of the shoot apical meristem. These cells give rise to peripheral derivatives from which lateral organ initials are recruited. During initial stages of cell recruitment, the three-dimensional form of lateral organs is specified. Lateral organs such as leaves develop and differentiate along proximodistal (base-to-tip), dorsoventral (top-to bottom) and mediolateral (middle-to-margin) planes. Current findings are refining our knowledge of the genes and genetic interactions that regulate these early processes and are providing a picture of how these pathways may contribute to variation in leaf form. 相似文献
162.
Byrne M 《The Biological bulletin》2005,208(2):81-91
Cryptasterina hystera has a highly derived life history with intragonadal development and juveniles that emerge from the parent's reproductive tract. The gonads are ovotestes with developing eggs separated from sperm by follicle cells. C. hystera has typical echinosperm that must enter the gonoduct of conspecifics to achieve fertilization. During oogenesis, an initial period of yolk accumulation is followed by hypertrophic lipid deposition, the major contributor to the increase in egg size. 1-Methyladenine induces egg maturation and ovulation, but the spawning component of the hormonal cascade is suppressed. This is the major alteration in reproduction associated with evolution of viviparity in C. hystera. The switch to viviparity was not accompanied by major change in gonad structure, indicating there were few or no anatomical constraints for evolution of a marsupial function for the gonad. Despite their intragonadal habitat, the brachiolaria are equipped for a planktonic life, swimming in gonadal fluid. During the gastrula stage, lipid provisions are released into the blastocoel where they are stored for juvenile development. The eggs of C. hystera have light and dark cytoplasmic regions that mark animal-vegetal polarity. The dark pigment provided a marker to follow the fate of vegetal cells. Live birth is rare in the Echinodermata and the incidence of this form of brooding in the phylum is reviewed. 相似文献
163.
Long runs of seasonal rotifer population data allow analysis of seasonal occurrence using mathematical tools. The application of Fourier analysis to a 15 year dataset describes seasonality in simple mathematical terms. This facilitates comparison of population expression with potential population driving variables and provides a basic modelling tool. Results show that annual patterns of occurrence and density have linkages with annual maximum and minimum environmental temperature, although the exact relationships are not clear. 相似文献
164.
Macrophages activate autophagy as an immediate response to Legionella pneumophila infection, but what marks the pathogen phagosome as a target for the autophagy machinery is not known. Because a variety of bacteria, parasites, viruses, and toxins that associate with the endoplasmic reticulum enter host cells by a cholesterol-dependent route, we tested the hypothesis that autophagy is triggered when microbes engage components of lipid raft domains. As the intracellular respiratory pathogen L. pneumophila or the extracellular uropathogen FimH(+) Escherichia coli entered macrophages by a cholesterol-sensitive mechanism, they immediatezly resided in vacuoles rich in glycosylphosphatidylinositol moieties and the autophagy enzyme Atg7. As expected for autophagosomes, the vacuoles sequentially acquired the endoplasmic reticulum protein BiP, the autophagy markers Atg8 and monodansyl-cadaverine, and the lysosomal protein LAMP-1. A robust macrophage response to the pathogens was cholesterol-dependent, since fewer Atg7-rich vacuoles were observed when macrophages were pretreated with methyl-beta-cyclodextrin or filipin. A model in which macrophages exploit autophagy to capture pathogens within the lipid raft pathway for antigen presentation prior to disposal in lysosomes is discussed. 相似文献
165.
Jung JC West-Mays JA Stramer BM Byrne MH Scott S Mody MK Sadow PM Krane SM Fini ME 《Journal of cellular physiology》2004,201(1):155-164
Prolactin (PRL) has long been implicated in Xenopus metamorphosis as an anti-metamorphic and/or juvenilizing hormone. Numerous studies showed that PRL could prevent effects of either endogenous or exogenous thyroid hormone (TH; T(3)). It has been shown that expression of matrix metalloproteinases (MMPs) is induced by TH during Xenopus metamorphosis. Direct in vivo evidence, however, for such anti-TH effects by PRL with respect to MMPs has not been available for the early phase of Xenopus development or metamorphosis. To understand the functional role of PRL, we investigated effects of PRL on Xenopus collagenase-3 (XCL3) and collagenase-4 (XCL4) expression in a cultured Xenopus laevis cell line, XL-177. Northern blot analysis demonstrated that XCL3 and XCL4 expression were not detected in control or T(3)-treated cells, but were differentially induced by PRL in a dose- and time-dependent fashion. Moreover, treatment with IL-1alpha as well as phorbol myristate acetate (PMA), a protein kinase C (PKC) activator, or H8, a protein kinase A (PKA) inhibitor, augmented PRL-induced collagenase expression, suggesting that multiple protein kinase pathways and cytokines may participate in PRL-induced collagenase expression. Interestingly, XCL3 expression could be induced in XL-177 cells by T(3), but only when co-cultured with prometamorphic Xenopus tadpole tails (stage 54/55), suggesting that the tails secrete a required intermediate signaling molecule(s) for T(3)-induced XCL3 expression. Taken together, these data demonstrate that XCL3 and XCL4 can be differentially induced by PRL and T(3) and further suggest that PRL is a candidate regulator of TH-independent collagenase expression during the organ/tissue remodeling which occurs in Xenopus development. 相似文献
166.
Byrne CJ Holland CV Walsh E Mulligan C Kennedy CR Poole WR 《Journal of helminthology》2004,78(3):201-206
The population biology of the fish acanthocephalan Acanthocephalus clavula was described from 161 wild brown trout, Salmo trutta sampled over a two-year period in Clogher Lake in the west of Ireland. Overall prevalence of the parasite was 86% and the mean abundance was 53 worms per fish. Despite the presence of large numbers of worms in the trout very few females (2%) attained full reproductive maturity. This suggests that trout is an accidental host. A sample of yellow eels, Anguilla anguilla was examined at a different time from the same lake. The prevalence of A. clavula was 97% and the average abundance was 8 worms per fish. In contrast to the situation in trout, the proportion of female worms attaining full reproductive maturity was 61% fulfilling the expected characteristic of a preferred definitive host. The possible explanations for the very high abundance of A. clavula in trout are discussed and include the influence of fluctuations in host populations, host diet and the absence of a potential competitor. 相似文献
167.
168.
Embryonic stem cells (ESCs) can proliferate indefinitely, maintain an undifferentiated pluripotent state and differentiate into any cell type. Differentiation of ESCs into various specific cell-types may be able to cure or alleviate the symptoms of various degenerative diseases. Unresolved issues regarding maintaining function, possible apoptosis and tumor formation in vivo mean a prudent approach should be taken towards advancing ESCs into human clinical trials. Rhesus macaques provide the ideal model organism for testing the feasibility, efficacy and safety of ESC based therapies and significant numbers of primate ESC lines are now available. In this review, we will summarize progress in evaluating the genetic and epigenetic integrity of primate ESCs, examine their current use in pre-clinical trials and discuss the potential of producing ESC-derived cell populations that are genetically identical (isogenic) to the host by somatic cell nuclear transfer. 相似文献
169.
170.
Extraordinary resistance to insecticides reveals exotic Q biotype of Bemisia tabaci in the New World
Dennehy TJ Degain BA Harpold VS Zaborac M Morin S Fabrick JA Nichols RL Brown JK Byrne FJ Li X 《Journal of economic entomology》2010,103(6):2174-2186
A strain of the whitefly Bemisia tabaci (Gennadius) possessing unusually high levels of resistance to a wide range of insecticides was discovered in 2004 in the course of routine resistance monitoring in Arizona. The multiply resistant insects, collected from poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch) plants purchased at a retail store in Tucson, were subjected to biotype analysis in three laboratories. Polyacrylamide gel electrophoresis of naphthyl esterases and sequencing of the mitochondrial cytochrome oxidase I gene (780 bp) confirmed the first detection of the Q biotype of B. tabaci in the New World. This U.S. Q biotype strain, referred to as Poinsettia'04, was highly resistant to two selective insect growth regulators, pyriproxyfen and buprofezin, and to mixtures of fenpropathrin and acephate. It was also unusually low in susceptibility to the neonicotinoid insecticides imidacloprid, acetamiprid, and thiamethoxam, relative to B biotype whiteflies. In 100 collections of whiteflies made in Arizona cotton (Gossypium spp.), vegetable, and melon (Cucumis melo L.) fields from 2001 to 2005, no Q biotypes were detected. Regions of the United States that were severely impacted by the introduction of the B biotype of B. tabaci in the 1980s would be well advised to promote measures that limit movement of the Q biotype from controlled environments into field systems and to formulate alternatives for managing this multiply-resistant biotype, in the event that it becomes more widely distributed. 相似文献