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901.
902.
Chi Hyun Kim Hee Young Kang Bo Ra Kim Hyejin Jeon Yoo Chul Lee Sang Hwa Lee Je Chul Lee 《Journal of microbiology (Seoul, Korea)》2016,54(1):44-49
This study investigated the mechanisms underlying the carbapenem resistance of bloodstream isolates of Pseudomonas aeruginosa obtained from two Korean hospitals. Of the 79 P. aeruginosa isolates, 22 and 21 were resistant to imipenem and meropenem, respectively. The 22 imipenem-resistant P. aeruginosa isolates were classified into 7 sequence types (STs) and 13 pulsotypes. Twelve imipenem-resistant isolates from one hospital were found to belong to the international clone ST111. Two imipenem-resistant P. aeruginosa ST235 isolates carried the bla IMP-6 gene, but the remaining 20 isolates did not produce carbapenemases. Mutations in the oprD gene and a related decrease in gene expression were found in 21 and 5 isolates, respectively. However, all imipenemresistant P. aeruginosa isolates showed no significant expression of OprD in the outer membrane as compared with that of carbapenem-susceptible PAO1 strain. Overexpression of genes associated with efflux pumps, including mexB, mexD, mexF, and mexY, was not found in any imipenem-resistant isolate. One imipenem-resistant P. aeruginosa isolate overexpressed the ampC gene. Our results show that the low permeability of drugs due to the mutational inactivation of OprD is primarily responsible for carbapenem resistance in bloodstream isolates of P. aeruginosa from Korean hospitals. 相似文献
903.
Jae-Bong Lee Seon Hwa Jeon Seok-Gwan Choi Hee-Young Jung Myung Kyum Kim Sathiyaraj Srinivasan 《Journal of microbiology (Seoul, Korea)》2016,54(12):809-813
In this paper, a new bacterial strain designated as 16MFT21T is isolated from the muscle of a fish caught in the Antarctic Ocean. Strain 16MFT21T is a Gram-staining-positive, catalase-oxidase-positive, rod-shaped facultative-aerobic bacterium. The phylogenetic analysis that is based on the 16S-rRNA gene sequence of strain 16MFT21T revealed that it belongs to the genus Bacillus in the family Bacillaceae in the class Bacilli. The highest degrees of the sequence similarity of the strain 16MFT21T is with Bacillus licheniformis ATCC 14580T (96.6%) and Bacillus sonorensis NBRC 101234T (96.6%). The isolate formed a pale-yellow pigment, and it grew in the presence of 0% to 10% (w/v) NaCl (optimum at 2% NaCl), a pH of 6.0 to 10.0 (optimum pH from 7.0 to 8.0), and from 4°C to 30°C (optimum at 30°C). The major polar lipids consist of diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG). The predominant fatty acids are iso-C15:0, anteiso-C15:0, iso-C17:0, and anteiso-C17:0. The main respiratory quinone is menaquinone-7 (MK-7), and based on the use of the meso-diaminopimelic acid as the diagnostic diamino acid, the peptidoglycan cell-wall type is A1γ. Based on the phylogenetic, phenotypic, and chemotaxonomic data, strain 16MFT21T (=KCTC 18866T =JCM 31664T) for which the name Bacillus piscis sp. nov. is proposed should be classified as a new species. 相似文献
904.
Jinmoon Kim Sungil Jang Aeryun Kim Hanfu Su Niluka Gunawardhana Yeong-Eui Jeon Eun Jung Bak Ji-Hye Kim Jeong-Heon Cha 《Journal of microbiology (Seoul, Korea)》2016,54(5):396-402
Mammalian γ-glutamyltranspeptidase (GGT) has been identified as a bone-resorbing factor. Since GGT of Bacillus subtilis exhibits similarity in their primary structure and enzymatic characteristics with mammalian GGTs, the bone-resorbing activity of bacterial GGT was examined in this study. Osteoclastogenesis was performed in a co-culture system of mouse calvaria-derived osteoblasts and bone marrow cells. A conditioned medium from GGT-overproducing B. subtilis culture showed significantly higher activity of osteoclast formation than a conditioned medium from wild-type B. subtilis culture. Recombinant GGT (rGGT) of wild-type B. subtilis and an enzymatic activity-defected rGGT of B. subtilis 2288 mutant were expressed in Escherichia coli and purified using His tag. Both purified rGGTs induced similar levels of osteoclastogenesis, suggesting that B. subtilis GGT possesses virulent bone-resorbing activity and its activity is probably independent of its enzymatic activity. Furthermore, a recombinant protein of B. subtilis GGT heavy subunit (Bs rGGT/H) showed strong activity of osteoclastogenesis while the light subunit failed to show strong activity, suggesting that the bone-resorbing activity is mainly located at the heavy subunit. More importantly, the GGT enzymatic activity may not be required for this virulence activity since the light subunit contains the catalytic pocket. In addition, B. subtilis rGGT stimulated mRNA expressions of receptor activator of nuclear factor kappa-B ligand (RANKL) and cyclooxygenase-2 (COX-2), while an osteoprotegerin inhibited the osteoclast formation induced by Bs rGGT/H. This is the first demonstration that bacterial GGT itself is sufficient to act as a bone-resorbing virulence factor via RANKL-dependent pathway. Therefore, it can be hypothesized that GGT of periodontopathic bacteria may play an important role as a virulence factor in bone destruction. 相似文献
905.
Yong-Joon Jeon Jin Hyun Kim Jong-Il Shin Mini Jeong Jaewook Cho Kyungho Lee 《Molecules and cells》2016,39(2):129-135
Eukaryotic translation initiation factor 2 alpha (eIF2α), which is a component of the eukaryotic translation initiation complex, functions in cell death and survival under various stress conditions. In this study, we investigated the roles of eIF2α phosphorylation in cell death using the breast cancer cell lines MCF-7 and MCF-7/ADR. MCF-7/ADR cells are MCF-7-driven cells that have acquired resistance to doxorubicin (ADR). Treatment of doxorubicin reduced the viability and induced apoptosis in both cell lines, although susceptibility to the drug was very different. Treatment with doxorubicin induced phosphorylation of eIF2α in MCF-7 cells but not in MCF-7/ADR cells. Basal expression levels of Growth Arrest and DNA Damage 34 (GADD34), a regulator of eIF2α, were higher in MCF-7/ADR cells compared to MCF-7 cells. Indeed, treatment with salubrinal, an inhibitor of GADD34, resulted in the upregulation of eIF2α phosphorylation and enhanced doxorubicin-mediated apoptosis in MCF-7/ADR cells. However, MCF-7 cells did not show such synergic effects. These results suggest that dephosphorylation of eIF2α by GADD34 plays an important role in doxorubicin resistance in MCF-7/ADR cells. 相似文献
906.
Hyun‐Bi Kim Dong‐Ho Shin Youn‐Il Park Yong‐Sic Hwang Sang‐Kyu Lee Gynheung An Jong‐Seong Jeon 《植物学报(英文版)》2016,58(2):127-135
We characterized the function of the rice cytosolic hexokinase Os HXK7(Oryza sativa Hexokinase7),which is highly upregulated when seeds germinate under O_2-deficient conditions. According to transient expression assays that used the promoter:luciferase fusion construct,Os HXK7 enhanced the glucose(Glc)-dependent repression of a rice a-amylase gene(RAmy3D) in the mesophyll protoplasts of maize,but its catalytically inactive mutant alleles did not. Consistently,the expression of Os HXK7,but not its catalytically inactive alleles,complemented the Arabidopsis glucose insensitive2-1(gin2-1) mutant,thereby resulting in the wild type characteristics of Glc-dependent repression,seedling development,and plant growth. Interestingly,Os HXK7-mediated Glc-dependent repression was abolished in the O_2-deficient mesophyll protoplasts of maize. This result provides compelling evidence that Os HXK7 functions in sugar signaling via a glycolysis-dependent manner under normal conditions,but its signaling role is suppressed when O_2 is deficient. The germination of two null Os HXK7 mutants,oshxk7-1 and oshxk7-2,was affected by O_2 deficiency,but overexpression enhanced germination in rice. This result suggests the distinct role that OsH XK7 plays in sugar metabolism and efficient germination by enforcing glycolysis-mediated fermentation in O_2-deficient rice. 相似文献
907.
908.
909.
Eradication Rates of Helicobacter pylori in Korea Over the Past 10 years and Correlation of the Amount of Antibiotics Use: Nationwide Survey
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Woon Geon Shin Sang Woo Lee Gwang Ho Baik Kyu Chan Huh Sang In Lee Jun‐Won Chung Woon Tae Jung Moo In Park Hye‐kyung Jung Heung Up Kim Jeong Hwan Kim Sang Young Seol Soon Man Yoon Seong Woo Jeon Su Jin Hong Gwang Ha Kim Dong Ho Lee Hyun Soo Kim Suck Chei Choi Hee Mo Kang Joongyub Lee Jae Gyu Kim Jae J. Kim 《Helicobacter》2016,21(4):266-278
910.
Jinhong Park Poonam Khloya Yohan Seo Satish Kumar Ho K. Lee Dong-Kyu Jeon Sungwoo Jo Pawan K. Sharma Wan Namkung 《PloS one》2016,11(2)
The most common mutation of CFTR, affecting approximately 90% of CF patients, is a deletion of phenylalanine at position 508 (F508del, ΔF508). Misfolding of ΔF508-CFTR impairs both its trafficking to the plasma membrane and its chloride channel activity. To identify small molecules that can restore channel activity of ΔF508-CFTR, we synthesized and evaluated eighteen novel hydroxypyrazoline analogues as CFTR potentiators. To elucidate potentiation activities of hydroxypyrazolines for ΔF508-CFTR, CFTR activity was measured using a halide-sensitive YFP assay, Ussing chamber assay and patch-clamp technique. Compounds 7p, 7q and 7r exhibited excellent potentiation with EC50 value <10 μM. Among the compounds, 7q (a novel CFTR potentiator, CP7q) showed the highest potentiation activity with EC50 values of 0.88 ± 0.11 and 4.45 ± 0.31 μM for wild-type and ΔF508-CFTR, respectively. In addition, CP7q significantly potentiated chloride conductance of G551D-CFTR, a CFTR gating mutant; its maximal potentiation activity was 1.9 fold higher than the well-known CFTR potentiator genistein. Combination treatment with CP7q and VX-809, a corrector of ΔF508-CFTR, significantly enhanced functional rescue of ΔF508-CFTR compared with VX-809 alone. CP7q did not alter the cytosolic cAMP level and showed no cytotoxicity at the concentration showing maximum efficacy. The hydroxypyrazolines may be potential development candidates for drug therapy of cystic fibrosis. 相似文献