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31.
Alessandro Bruni Maria P. Fasulo Barbara Tosi Giuseppe Dall'Olio Gian Luigi Vannini 《Histochemistry and cell biology》1976,48(4):269-281
Summary The new highly sensitive method of fluorescamine reaction for the topochemical detection of primary amino groups was studied as a substitude of ninhydrin-Schiff's reaction for the localisation of total proteins in plant tissues. The influence of various coagulant and non-coagulatn fixatives on the induction of fluorescamine fluorescence was examined: ethanol, formaldehyde gas and solution, glutaraldehyde, acrolein, osmium tetroxide, Bouin, Rossman, Clarke and Zenker's fluids and FMA were employed. It was found that the use of the fluorogenic method is conditioned by the fixative ability to keep the amino groups disposable and by its capability to reduce the natural autofluorescence of plant material. A detailed account of the fixation methodology demonstrated that non-coagulant acrolein and coagulant mercuric chloride are the most promising fixatives for the use of the fluorescamine reaction in plant histochemistry. 相似文献
32.
Hdmx recruitment into the nucleus by Hdm2 is essential for its ability to regulate p53 stability and transactivation. 总被引:4,自引:0,他引:4
33.
Jenny Anne Glikman Jerry J. Vaske Alistair J. Bath Paolo Ciucci Luigi Boitani 《European Journal of Wildlife Research》2012,58(1):295-302
This article examines the combined influence of cognitions (i.e., impact beliefs) and affect (i.e., feelings) on normative
beliefs (i.e., support for management options) about wolves and brown bears. Data were obtained from stratified random face-to-face
interviews (n = 1,611). The survey was conducted in the Abruzzo Lazio and Molise National Park (central Italy), where people have a long
history of coexistence with large carnivores. Knowledge was hypothesized to moderate the relationships of beliefs and feelings
on support for management actions. Path analyses supported the role of affect in mediating perceived impact beliefs and support
for the protection of large carnivores. Knowledge moderated these relationships in the case of wolves but not brown bears.
Residents of the national park had more knowledge about bears than wolves, which might partly explain both the stronger effect
that knowledge had on the affective component and its lack of a moderating effect on the bear model. Overall, our findings
show the positive attitude of residents toward large carnivores and support the idea of affect being more important than cognition
in predicting normative beliefs. 相似文献
34.
Preferred proline puckerings in cis andtrans peptide groups: Implications for collagen stability 下载免费PDF全文
Luigi Vitagliano Rita Berisio Antonio Mastrangelo Lelio Mazzarella Adriana Zagari 《Protein science : a publication of the Protein Society》2001,10(12):2627-2632
The interplay between side-chain and main-chain conformations is a distinctive characteristic of proline residues. Here we report the results of a statistical analysis of proline conformations using a large protein database. In particular, we found that proline residues with the preceding peptide bond in the cis state preferentially adopt a down puckering. Indeed, out of 178 cis proline residues, as many as 145 (81%) are down. By analyzing the 1-4 and 1-5 nonbonding distances between backbone atoms, we provide a structural explanation for the observed trend. The observed correlation between proline puckering and peptide bond conformation suggests a new mechanism to explain the reported shift of the cis-trans equilibrium in proline derivatives. The implications of these results for the current models of collagen stability are also discussed. 相似文献
35.
36.
Yu PJ Ferrari G Pirelli L Gulkarov I Galloway AC Mignatti P Pintucci G 《Cellular signalling》2007,19(7):1359-1371
Cardiovascular interventions that restore blood circulation to ischemic areas are accompanied by significant tissue damage, which triggers a vascular remodeling response that may result in restenosis of blood conduits. Early endothelial dysfunction and/or impairment is the early event of a cascade that leads, through an inflammatory response and dedifferentiation of medial smooth muscle cells with abundant deposition of extracellular matrix, to intimal hyperplasia. Here we present the molecular and cellular mechanisms of intimal hyperplasia secondary to vascular injury and discuss the potential role of therapeutic modulation of the intracellular signaling pathways that differentially effect vascular endothelial and smooth muscle cells. The role of mitogen-activated protein kinases (MAPKs) and the outcome of their modulation in these processes are highlighted here as they provide a promising therapeutic target for prevention of restenosis. 相似文献
37.
38.
Elisabetta Gianazza Francesco Chillemi Marcello Duranti Pier Giorgio Righetti 《Journal of biochemical and biophysical methods》1983,8(4):339-351
A new method for peptide analysis and purification is described, based on isoelectric focusing in immobilized pH gradients. On the analytical scale, the peptide zones can now be revealed by an stain for primary and secondary amino group (e.g. ninydrin, fluorescamine, dansyl chloride) since the buffering species, unlike conventional carrier ampholytes, contain only carboxyl and tertiary amino groups. For preparative purposes, conditions have been described to remove most contaminants (e.g. unreacted monomers, non-cross-linked, short polyacrylamide chains) from the gel matrix before the electrophoretic run. However, ca. 2% of the gel dry mass is still present as extractable material. The focused peptides can be recovered in higly yields (ca. 90%) with a fairly high degree of purity (75%), the contaminants being mostly components eluted from the polyacrylamide gel. 相似文献
39.
Pier Sandro Cocconcelli Elena Ferrari Filippo Rossi Vittorio Bottazzi 《FEMS microbiology letters》1992,94(3):203-207
To apply recombinant DNA techniques to the genetic manipulation of cellulolytic ruminal bacteria, a plasmid vector transformation system must be available. The objective of this work was to develop a system for plasmid transformation of Ruminococcus albus. Using high voltage electrotransformation, pSC22 and pCK17 plasmid vectors, derived from lactic acid bacteria plasmids and replicating via single-stranded DNA intermediate, were successfully introduced into three freshly isolated R. albus strains and into R. albus type strain ATCC 27210. The optimization of the electrotransformation condition raised the electroporation efficiency up to 3 x 10(5) transformants per microgram of pSC22 plasmid. 相似文献
40.
Raffaele De Vita Delia Cavallo Luigi Raganella Patrizia Eleuteri Maria G. Grollino Alberto Calugi 《Bioelectromagnetics》1995,16(5):330-334
Flow cytometry (FCM) was performed to monitor the cellular effects of extremely-low-frequency magnetic field on mouse spermatogenesis. Groups of five male hybrid F1 mice aged 8–10 weeks were exposed to 50 Hz magnetic field. The strength of the magnetic field was 1.7 mT. Exposure times of 2 and 4 h were chosen. FCM measurements were performed 7, 14, 21, 28, 35, and 42 days after treatment. For each experimental point, a sham-treated group was used as a control. The possible effects were studied by analyzing the DNA content distribution of the different cell types involved in spermatogenesis and using the elongated spermatids as the reference population. The relative frequencies of the various testicular cell types were calculated using specific software. In groups exposed for 2 h, no effects were observed. In groups exposed for 4 h, a statistically significant (P < 0.001) decrease in elongated spermatids was observed at 28 days after treatment. This change suggests a possible cytotoxic and/or cytostatic effect on differentiating spermatogonia. However, further studies are being carried out to investigate the effects of longer exposure times. © 1995 Wiley-Liss, Inc. 相似文献