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131.
Sterilization of bioreactor media on the basis of computer calculated thermal input designated asF
0
LaVerne D. Boeck Roger W. Wetzel Stanton C. Burt Floyd M. Huber Gary L. Fowler Joseph S. Alford Jr. 《Journal of industrial microbiology & biotechnology》1988,3(5):305-310
Summary Industrial fermentation media are normally sterilized with steam to destroy the indigenous microbial population prior to inoculation with a specific microorganism. Because biological validation of each sterilization cycle is impractical, an overkill approach is commonly employed on the basis that alteration of heat-sensitive nutrients is less detrimental than survival of indigenous microbes. However, the heat destruction of microbes is known to be a probability function amenable to calculation. A computer has been programmed to calculate the on-line heat input asF
0 values during sterilization of media in stirred bioreactors. The accumulation ofF
0 values is then announced verbally to bioreactor operators by a communications controller with voice synthesizer. 相似文献
132.
B M Goudie A D Burt P Boyle G Macfarlane G G Birnie P R Mills C R Gillis R N MacSween G Watkinson 《BMJ (Clinical research ed.)》1985,291(6509):1597-1598
The occurrence of extrahepatic malignancy was studied in 195 unselected patients who satisfied predetermined biochemical, immunological, and histological criteria for the diagnosis of primary biliary cirrhosis. The incidence of breast cancer in women with primary biliary cirrhosis was found to be significantly higher than in an age and sex matched control population from the same well defined geographical area (p less than 0.0015). The association of breast cancer and primary biliary cirrhosis remains unexplained, though diminished immunological surveillance, fat soluble vitamin deficiency, or endocrine dysfunction may play a part. 相似文献
133.
Benjamin C. C. Hume Cecilia D'Angelo Edward G. Smith Jamie R. Stevens John A. Burt Jörg Wiedenmann 《Journal of phycology》2018,54(5):762-764
The binary designation Symbiodinium thermophilum was invalid due to the absence of an illustration as required by Article 44.2 of the ICN. Herein, it is validated. This species is the most common symbiont in reef corals in the southern Persian/Arabian Gulf, the world's hottest body of water sustaining reef coral growth. 相似文献
134.
Wendy Pyper Michael Burt Lawrie Powell Sonja Webb Lesley Adès June Halliday Elizabeth Jazwinska 《Human genetics》1995,96(4):490-492
D6S265 is a polymorphic dinucleotide repeat, mapped within 70 kb centromeric of HLA-A, on chromosome 6p21.3. While genotyping families for genetic linkage analysis, allele non-amplification resulting in apparent non-Mendelian inheritance was observed at the D6S265 locus in 15 individuals, on chromosomes carrying the HLA-A25 and HLA-A26 antigens. The D6S265 locus was sequenced in a variant individual homozygous for allele non-amplification, and in a non-HLA-A25/-A26 individual, homozygous for D6S265 allele 1. Five base changes were identified in the reverse primer binding region of the variant individual, effectively preventing annealing of the 3 primer to the template. 相似文献
135.
Comparative Genome Organization of Vertebrates 总被引:13,自引:0,他引:13
L. Andersson A. Archibald M. Ashburner S. Audun W. Barendse J. Bitgood C. Bottema T. Broad S. Brown D. Burt C. Charlier N. Copeland S. Davis M. Davisson J. Edwards A. Eggen G. Elgar J. T. Eppig I. Franklin P. Grewe T. Gill J. A. M. Graves R. Hawken J. Hetzel A. Hilyard H. Jacob L. Jaswinska N. Jenkins H. Kunz G. Levan O. Lie L. Lyons P. Maccarone C. Mellersh G. Montgomery S. Moore C. Moran D. Morizot M. Neff F. Nicholas S. O’Brien Y. Parsons J. Peters J. Postlethwait M. Raymond M. Rothschild L. Schook Y. Sugimoto C. Szpirer M. Tate J. Taylor J. VandeBerg M. Wakefield J. Wienberg J. Womack 《Mammalian genome》1996,7(10):717-734
136.
137.
Cephapirin, a new semisynthetic cephalosporin derivative, was found to have an antibacterial spectrum similar to that of cephalothin. Staphylococcus aureus was inhibited by cephapirin concentrations of 0.09 to 12.5 mug/ml. S. epidermidis, S. viridans, S. pyogenes, and Diplococcus pneumonia isolates were inhibited by less than 1 mug/ml. The Enterococcus required a concentration of 25 mug of antibiotic per ml for inhibition. Approximately 65% of Escherichia coli, and all Klebsiella, indole-negative Proteus, and Salmonella strains tested were inhibited by the drug. Serratia, Pseudomonas, indole-positive Proteus, and Erwinia strains were highly resistant. Inoculum size was not an important factor in determining the level of sensitivity of S. aureus to cephapirin. The antibiotic does not appear to be significantly bound to serum protein. In vitro development of resistance to the drug was demonstrated with two isolates of S. aureus. 相似文献
138.
Function of calmodulin in postsynaptic densities. II. Presence of a calmodulin- activatable protein kinase activity
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Because the calmodulin in postsynaptic densities (PSDs) activates a cyclic nucleotide phosphodiesterase, we decided to explore the possibility that the PSD also contains a calmodulin-activatable protein kinase activity. As seen by autoradiographic analysis of coomassie blue-stained SDS polyacrylamide gels, many proteins in a native PSD preparation were phosphorylated in the presence of [γ-(32)P]ATP and Mg(2+) alone. Addition of Ca(2+) alone to the native PSD preparation had little or no effect on phosphorylation. However, upon addition of exogenous calmodulin there was a general increase in background phosphorylation with a statistically significant increase in the phosphorylation of two protein regions: 51,000 and 62,000 M(r). Similar results were also obtained in sonicated or freeze thawed native PSD preparations by addition of Ca(2+) alone without exogenous calmodulin, indicating that the calmodulin in the PSD can activate the kinase present under certain conditions. The calmodulin dependency of the reaction was further strengthened by the observed inhibition of the calmodulin-activatable phosphorylation, but not of the Mg(2+)-dependent activity, by the Ca(2+) chelator, EGTA, which also removes the calmodulin from the structure (26), and by the binding to calmodulin of the antipsychotic drug chlorpromazine in the presence of Ca(2+). In addition, when a calmodulin-deficient PSD preparation was prepared (26), sonicated, and incubated with [γ-(32)P]ATP, Mg(2+) and Ca(2+), one could not induce a Ca(2+)-stimulation of protein kinase activity unless exogenous calmodulin was added back to the system, indicating a reconstitution of calmodulin into the PSD. We have also attempted to identify the two major phosphorylated proteins. Based on SDS polyacrylamide gel electrophoresis, it appears that the major 51,000 M(r) PSD protein is the one that is phosphorylated and not the 51,000 M(r) component of brain intermediate filaments, which is a known PSD contaminant. In addition, papain digestion of the 51,000 M(r) protein revealed multiple phosphorylation sites different from those phosphorylated by the Mg(2+)-dependent kinase(s). Finally, although the calmodulin-activatable protein kinase may phosphorylate proteins I(a) and I(b), the cyclic AMP-dependent protein kinase, which definitely does phosphorylate protein I(a) and I(b) and is present in the PSD, does not phosphorylate the 51,000 and 62,000 M(r) proteins, because specific inhibition of this kinase has no effect on the levels of the phosphorylation of these latter two proteins. 相似文献
139.
The pH dependence of the reaction of tris(hydroxymethyl)aminomethane (Tris) with the activated carbonyl compound 4-trans-benzylidene-2-phenyloxazolin-5-one (I) is given by the equation , where Ka is the dissociation constant of TrisH+. Spectrophotometric experiments show that the Tris ester of α-benzamido-trans-cinnamic acid is formed quantitatively over a range of pH values, regardless of the relative contribution of kb and ka terms to k′2. Hence, both terms refer to alcoholysis. While the mechanism of the reaction is not determined unequivocally in the present work, the magnitude of the kb term, together with its dependence on the basic form of Tris, suggests that ester formation is occurring by nucleophilic attack of a Tris hydroxyl group on the carbonyl carbon of the oxazolinone, with intramolecular catalysis by the Tris amino group. The rate enhancement due to this group is at least 102 and possibly of the order 106. This system is compared with other model systems for the acylation step of catalysis by serine esterases and proteinases. 相似文献
140.
Localization of the increased [32P]-labelling of phosphatidylinositol, caused by arrival of presynaptic impulses in a sympathetic ganglion, was investigated by subcellular fractionation of ganglia that had been labelled before homogenization. Paired superior cervical ganglia were excised from adult rats and incubated with 32P1 and [methyl-14C]choline for 4 h at 37°C. The preganglionic nerve of one ganglion of each pair was stimulated repetitively (10/s) during the last 3 h of incubation. The ganglia were then softened with collagenase, homogenized, and fractionated by density gradient centrifugation. Succinate dehydrogenase served as marker for the distribution of mitochondria on the gradient, and [14C]ACh for synaptosomes. [32P]-labelling of lipids was measured relative to that of phosphatidylcholine. The average changes in relative labelling that were caused by neuronal activity ranged as follows over the homogenate and the various fractions: phosphatidylinositol (PI), increased 39–75%; phosphatidylethanolamine, decreased 10–20%; diphosphatidylglycerol, not significantly affected. The increase in PI labelling was much greater in the denser fractions, in which synaptosomes and mitochondria were concentrated, than in the less dense fractions. The distribution of the PI effect on the gradient could be reasonably well explained by assuming that synaptosomes and mitochondria both contributed to the increase in PI labelling in proportion to the amount of their respective markers in a fraction. One-third or more of the total increase could thus be associated with synaptosomes and one-third or more with mitochondria, although alternative association with other structures could not be excluded. The implications of the inferred association with synaptosomes and mitochondria are discussed, current knowledge of the PI effect caused by impulses entering sympathetic ganglia is summarized, and suggestions concerning its physiological significance are reviewed. It is concluded that PI may have multiple roles in neuronal activity. 相似文献