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101.
102.
A. M. Efremov I. V. Dukhovlinov E. B. Dizhe S. V. Burov M. V. Leko B. N. Akifiev D. A. Mogilenko I. A. Ivanov A. P. Perevozchikov S. V. Orlov 《Cell and Tissue Biology》2010,4(4):362-371
The rate and character of superficial tissue regeneration after wounds, burns, and other traumas depend on cell proliferation
within the damaged area. The acceleration of wound healing via the stimulation of cell proliferation and extracellular matrix
synthesis is one of the most important tasks of modern medicine. There are gene therapy approaches to wound treatment, such
as the transfer of genes that encode mitogenic growth factors to the wound area. The most important step in the development
of the gene therapy approaches is the design of gene delivery tools. Despite the high efficiency of viral vectors, the nonviral
approaches have some advantages (low toxicity, low immunogenity, safety, and the absence of side effects). Among the nonviral
gene delivery tools molecular conjugates are the most popular due to their efficiency, simplicity, and the capacity for targeted
gene transfer. In the present work, we have developed two molecular conjugates, NLS-TSF7 and NLS-TSF12, which consist of the
modified signal of the nuclear localization of the T-antigen of the SV40 virus (cationic part) and the peptide ligands of
the mammalian transferrin receptor (ligand part). Those conjugates bind to plasmid DNA via the formation of polyelectrolytic
complexes and are able to deliver plasmid DNA into cells that express transferrin receptors through receptor-mediated endocytosis.
The transfer of the expression vector of the luciferase gene in the complex with the molecular conjugate NLS-TSF7 to murine
surface tissues led to about the 100-fold increase of luciferase activity in comparison with the transfer of the free expression
vector. The treatment of mice with incised wounds with complexes of the expression vector of the synthetic human gene that
encodes insulin-like growth factor 1 with molecular conjugate NLS-TSF7 led to the acceleration of wound healing in comparison
with mice treated with the free expression vector. The obtained results confirm the high efficiency of the developed approach
to regenerative gene therapy for treating the superficial tissue damage of mammals. 相似文献
103.
V I Kirpatovski? V N Burov Iu V Kudriavtsev 《Biulleten' eksperimental'no? biologii i meditsiny》1992,114(10):434-437
The aim of the study is investigation of possibility to bypass small and medium-size arteries with cryopreserved artery allografts, storing 7-10 days at -196 degrees C under the protection of 15% dimethylsulfoxide. In experiments on 40 rabbits were placed a region of the left renal artery by cryopreserved bioprosthesis. Graft patency was 80% after observation up to 6 months. By angiography it was 8 cases of graft thrombosis (all during the 1st week after implantation) and 5 cases of moderate graft dilation (in 4 of them it was accompanied with stenosis of distal anastomosis). In 20 dogs we replaced a region of the femoral artery by cryostoring bioprosthesis. It was only one case of graft thrombosis which occurred on month 2 after the implantation during 1-year follow-up. After 3 months in 3 cases there developed 3 cases of diffuse narrowing of graft lumen without decreasing of blood flow through the prosthesis. Later, the graft lumen did not change. Histological investigations have revealed a viability of cryopreserved vessels, its almost complete de-endothelialization at 3 days and total re-endothelialization 2 weeks after implantation. During the first 2 weeks there were morphological events of graft rejection, which disappeared after 3 months. 相似文献
104.
A. M. Efremov A. O. Buglaeva S. V. Orlov S. V. Burov I. A. Ignatovich E. B. Dizhe V. S. Shavva A. P. Perevozchikov 《Russian Journal of Developmental Biology》2010,41(2):71-76
Genetic modification of mammalian embryos is an important way to model various changes in human development; also, it is an
instrument for studying the functions of certain genes in mammals. Using our own experience in developing modes of delivery
of genetic constructions to mammals in a nonviral way, we present here data on the delivery of a eukaryotic expression vector
to mice embryos through the transpla-cental barrier with the use of hydrodynamic intravenous injections of DNA-hybrid peptide
complexes to pregnant females. The peptide has a cationic part for interaction with DNA and includes a ligand structure towards
receptors of the releasing factor of luteinizing hormone (RFLH, luliberin). Advantages of the suggested method are simplicity,
economy, nonimmunogenicity for females, and the ability to multiply repeat the procedure. On the basis of the method, systemic
gene delivery into tissues of mammalian embryos may be developed. 相似文献
105.
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109.
D. I. Kuzmenko P. G. Burov V. Yu. Serebrov E. A. Fait T. V. Perevozchikova 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2011,5(4):376-380
A functional state of the sphingomyeline cycle and its links with processes of free radical lipid oxidation have been investigated
in rat liver during starvation of animals without any restriction of access to drinking water at day 1, 2, 3 (phase I) and
day 6 (phase II of starvation). The maximal values of the ceramide/sphingomyelin ratio and activities of neutral sphingomyelinase
and executive caspase-3 were found in rat livers at day 3 of starvation. Since day 3 of starvation an increase of tumour necrosis
factor-α, one of neutral sphingomyelinase activators, was detected in serum. During the major part of phase I of starvation
the intensity of liver free radical lipid peroxidation was comparable to that of control due to competent functioning of the
antioxidant defense system. Transition of phase I to phase II of starvation (day 6 of the experiment) was accompanied by the
development of oxidative stress associated with depletion of the antioxidant defense system. The results obtained in this
study suggest that phase I of starvation favors realization of the ceramide-mediated proapoptotic signaling in the liver.
In our viewpoint, ceramide-mediated apoptosis is one of mechanisms used optimization of liver cellular population within the
frame of metabolic adaptation. In rat liver phase I of starvation was characterized by prevailing of the ceramide-mediated
proapoptotic signaling, while in phase II oxidative stress dominated. 相似文献
110.
Funikov S. Yu. Spasskaya D. S. Burov A. V. Teterina E. V. Ustyugov A. A. Karpov V. L. Morozov A. V. 《Molecular Biology》2021,55(1):47-55
Molecular Biology - Proteasomes are multisubunit complexes that degrade most intracellular proteins. Three of the 14 subunits of the 20S proteasome, specifically β1, β2, and... 相似文献