全文获取类型
收费全文 | 195篇 |
免费 | 15篇 |
国内免费 | 1篇 |
出版年
2021年 | 4篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 8篇 |
2014年 | 8篇 |
2013年 | 7篇 |
2012年 | 9篇 |
2011年 | 10篇 |
2010年 | 12篇 |
2009年 | 12篇 |
2008年 | 9篇 |
2007年 | 9篇 |
2006年 | 6篇 |
2005年 | 3篇 |
2004年 | 4篇 |
2003年 | 6篇 |
2002年 | 3篇 |
2001年 | 9篇 |
2000年 | 4篇 |
1998年 | 10篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1994年 | 2篇 |
1993年 | 7篇 |
1990年 | 2篇 |
1987年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 3篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1971年 | 2篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1964年 | 2篇 |
1962年 | 1篇 |
1961年 | 2篇 |
1957年 | 1篇 |
1956年 | 2篇 |
1946年 | 1篇 |
1943年 | 1篇 |
1941年 | 1篇 |
排序方式: 共有211条查询结果,搜索用时 15 毫秒
121.
122.
123.
124.
125.
126.
I Prieto-Potín JA Roman-Blas MJ Martínez-Calatrava R Gómez R Largo Gabriel Herrero-Beaumont 《Arthritis research & therapy》2013,15(4):R81
Objective
The aim of this study was to determine whether hypercholesterolemia increases articular damage in a rabbit model of chronic arthritis.Methods
Hypercholesterolemia was induced in 18 rabbits by administrating a high-fat diet (HFD). Fifteen rabbits were fed normal chow as controls. Chronic antigen-induced arthritis (AIA) was induced in half of the HFD and control rabbits, previously immunized, by intra-articular injections of ovalbumin. After sacrifice, lipid and systemic inflammation markers were analyzed in blood serum. Synovium was analyzed by Krenn score, multinucleated cell counting, immunohistochemistry of RAM11 and CD31, and TNF-α and macrophage chemoattractant protein-1 (MCP-1) gene expression. Active bone resorption was assessed by protein expression of receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG) and quantification of cathepsin K, contact surface and the invasive area of pannus into bone.Results
Rabbits receiving the HFD showed higher total serum cholesterol, HDL, triglycerides and CRP levels than rabbits fed a normal diet. Synovitis score was increased in HFD, and particularly in AIA and AIA + HFD groups. AIA + HFD synovium was characterized by a massive infiltration of RAM11+ cells, higher presence of multinucleated foam cells and bigger vascularization than AIA. Cathepsin K+ osteoclasts and the contact surface of bone resorbing pannus were also increased in rabbits with AIA + HFD compared with AIA alone. Synovial TNF-α and MCP-1 gene expression was increased in AIA and HFD rabbits compared with healthy animals. RANKL protein expression in AIA and AIA + HFD groups was higher compared with either HFD or normal groups.Conclusions
This experimental model demonstrates that hypercholesterolemia increments joint tissue damage in chronic arthritis, with foam macrophages being key players in this process. 相似文献127.
Impacts of yeast metabolic network structure on enzyme evolution 总被引:1,自引:1,他引:0
A comment on D Vitkup, P Kharchenko and A Wagner: Influence of metabolic network structure and function on enzyme evolution. Genome Biol 2006, 7:R39. 相似文献
128.
BD Pascal MJ Chalmers SA Busby CC Mader MR Southern NF Tsinoremas PR Griffin 《BMC bioinformatics》2007,8(1):156
Background
The combination of mass spectrometry and solution phase amide hydrogen/deuterium exchange (H/D exchange) experiments is an effective method for characterizing protein dynamics, and protein-protein or protein-ligand interactions. Despite methodological advancements and improvements in instrumentation and automation, data analysis and display remains a tedious process. The factors that contribute to this bottleneck are the large number of data points produced in a typical experiment, each requiring manual curation and validation, and then calculation of the level of backbone amide exchange. Tools have become available that address some of these issues, but lack sufficient integration, functionality, and accessibility required to address the needs of the H/D exchange community. To date there is no software for the analysis of H/D exchange data that comprehensively addresses these issues. 相似文献129.
130.
ON and OFF retinal ganglion cells (RGCs) display differences in their intrinsic electrophysiology: OFF cells maintain spontaneous
activity in the absence of any input, exhibit subthreshold membrane potential oscillations, rebound excitation and burst firing;
ON cells require excitatory input to drive their activity and display none of the aforementioned phenomena. The goal of this
study was to identify and characterize ionic currents that explain these intrinsic electrophysiological differences between
ON and OFF RGCs. A mathematical model of the electrophysiological properties of ON and OFF RGCs was constructed and validated
using published patch-clamp data from isolated intact mouse retina. The model incorporates three ionic currents hypothesized
to play a role in generating behaviors that are different between ON and OFF RGCs. These currents are persistent Na + , I
NaP, hyperpolarization-activated, I
h, and low voltage activated Ca2 + , I
T, currents. Using computer simulations of Hodgkin-Huxley type neuron with a single compartment model we found two distinct
sets of I
NaP, I
h, I
T conductances that correspond to ON and OFF RGCs populations. Simulations indicated that special properties of I
T explain the differences in intrinsic electrophysiology between ON and OFF RGCs examined here. The modelling shows that the
maximum conductance of I
T is higher in OFF than in ON cells, in agreement with recent experimental data. 相似文献