全文获取类型
收费全文 | 1068篇 |
免费 | 90篇 |
出版年
2020年 | 9篇 |
2018年 | 10篇 |
2016年 | 18篇 |
2015年 | 23篇 |
2014年 | 31篇 |
2013年 | 36篇 |
2012年 | 46篇 |
2011年 | 56篇 |
2010年 | 44篇 |
2009年 | 26篇 |
2008年 | 32篇 |
2007年 | 36篇 |
2006年 | 32篇 |
2005年 | 35篇 |
2004年 | 35篇 |
2003年 | 38篇 |
2002年 | 25篇 |
2001年 | 36篇 |
2000年 | 33篇 |
1999年 | 29篇 |
1998年 | 17篇 |
1997年 | 9篇 |
1996年 | 12篇 |
1995年 | 16篇 |
1993年 | 10篇 |
1992年 | 17篇 |
1991年 | 18篇 |
1990年 | 26篇 |
1989年 | 18篇 |
1988年 | 18篇 |
1987年 | 24篇 |
1986年 | 17篇 |
1985年 | 24篇 |
1984年 | 10篇 |
1983年 | 16篇 |
1982年 | 17篇 |
1981年 | 10篇 |
1980年 | 10篇 |
1979年 | 21篇 |
1978年 | 8篇 |
1977年 | 9篇 |
1976年 | 16篇 |
1975年 | 14篇 |
1974年 | 17篇 |
1973年 | 21篇 |
1972年 | 12篇 |
1971年 | 11篇 |
1970年 | 10篇 |
1968年 | 9篇 |
1966年 | 8篇 |
排序方式: 共有1158条查询结果,搜索用时 46 毫秒
141.
Roger J. M. Brüggemann Jan-Willem C. Alffenaar Nicole M. A. Blijlevens Eliane M. Billaud Jos G. W. Kosterink Paul E. Verweij David M. Burger 《Current fungal infection reports》2008,2(1):20-27
Drug-drug interactions are a major concern with triazoles. Azoles are potent metabolic inhibitors and interactions commonly
occur via metabolizing enzymes (ie, cytochrome P450 isoenzyme superfamily) or drug transporters (ie, P-glycoprotein). However,
the clinical relevance of these interactions may vary upon the azole involved and upon the “target” drug. Azoles may also
be under influence of and become targets of metabolic drug-drug interactions. Potential interactions between antifungal agents
and over-the-counter or alternative medicines and herbs should not be underestimated. Here, we provide a comprehensive overview
of different types of pharmacokinetic drug interactions involving azoles with selected examples. 相似文献
142.
Burger C Zhou HW Wang H Sics I Hsiao BS Chu B Graham L Glimcher MJ 《Biophysical journal》2008,95(4):1985-1992
Combined small-angle x-ray scattering and transmission electron microscopy studies of intramuscular fish bone (shad and herring) indicate that the lateral packing of nanoscale calcium-phosphate crystals in collagen fibrils can be represented by irregular stacks of platelet-shaped crystals, intercalated with organic layers of collagen molecules. The scattering intensity distribution in this system can be described by a modified Zernike-Prins model, taking preferred orientation effects into account. Using the model, the diffuse fan-shaped small-angle x-ray scattering intensity profile, dominating the equatorial region of the scattering pattern, could be quantitatively analyzed as a function of the degree of mineralization. The mineral platelets were found to be very thin (1.5 nm ∼ 2.0 nm), having a narrow thickness distribution. The thickness of the organic layers between adjacent mineral platelets within a stack is more broadly distributed with the average value varying from 6 nm to 10 nm, depending on the extent of mineralization. The two-dimensional analytical scheme also leads to quantitative information about the preferred orientation of mineral stacks and the average height of crystals along the crystallographic c axis. 相似文献
143.
The role of VASP in regulation of cAMP- and Rac 1-mediated endothelial barrier stabilization 总被引:2,自引:0,他引:2
Schlegel N Burger S Golenhofen N Walter U Drenckhahn D Waschke J 《American journal of physiology. Cell physiology》2008,294(1):C178-C188
Regulation of actin dynamics is critical for endothelial barrier functions. We provide evidence that the actin-binding protein vasodilator-stimulated phosphoprotein (VASP) is required for endothelial barrier maintenance. Baseline permeability was significantly increased in VASP-deficient (VASP(-/-)) microvascular myocardial endothelial cells (MyEnd) in the absence of discernible alterations of immunostaining for adherens and tight junctions. We tested whether VASP is involved in the endothelium-stabilizing effects of cAMP or Rac 1. Forskolin and rolipram (F/R) to increase cAMP and cytotoxic necrotizing factor 1 (CNF-1) to activate Rac 1 were equally efficient to stabilize barrier functions in VASP(-/-) and wild-type (wt) cells. In wt cells, VASP was phosphorylated in response to F/R but did not localize to intercellular junctions. In contrast, CNF-1 and expression of constitutively active Rac 1 induced translocation of VASP to cell borders in wt cells, where it colocalized with active Rac 1. In VASP(-/-) cells, Rac 1 activity was reduced to 0.4 of wt levels in controls and increased approximately 20-fold in response to CNF-1 compared with 7-fold activation in wt cells. Moreover, inactivation of Rac 1 by lethal toxin led to a greater increase of permeability compared with wt cells. All these data suggest that VASP is involved in the regulation of Rac 1 activity. Taking these findings together, our study indicates that VASP at least in part stabilizes endothelial barrier functions by control of Rho-family GTPases. 相似文献
144.
145.
Potamoplankton is often a well developed component in large lowland rivers, yet little is known about its structure in New Zealand's longest river, the Waikato River. To redress this gap we sampled bimonthly at seven sites along the length of the river over 12 months. Rotifers were the dominant zooplankton in the Waikato River making up 85% of the total densities. Cladocerans represented 9% and copepods only 6%. Rotifers were also the most taxonomically rich group with 41 species in 20 genera identified throughout the study. Thirty rotifer species and nine genera represent new records for the river – two cladoceran species were also recorded for the first time. The highest densities of crustaceans and rotifers were found in the hydro lakes. Densities of crustaceans decreased with increasing distance downstream and densities of rotifers were on average 15 times greater than crustaceans in the lower river. The seasonality of Crustacea was similar to that in New Zealand lakes and rivers with high densities in summer and minimum densities over the winter period. Total rotifer densities showed a similar trend although there were marked seasonal differences between individual species. 相似文献
146.
Basic fibroblast growth factor modulates the expression of glycophorin A and c-kit and inhibits erythroid differentiation in K562 cells. 总被引:1,自引:0,他引:1
Patricia E Burger Pauline T Lukey Sandra Coetzee E Lynette Wilson 《Journal of cellular physiology》2002,190(1):83-91
Basic fibroblast growth factor (bFGF) is produced by bone marrow stromal cells as well as by normal and leukemic hematopoietic cells. In this study, we examine the direct effects of bFGF on erythroid differentiation in K562 cells in order to determine whether bFGF can promote the expression of a primitive phenotype. Low levels of bFGF inhibited erythroid differentiation as evidenced by decreased expression of glycophorin A and increased expression of c-kit. bFGF also increased both the numbers and the sizes of colonies of K562 cells in soft agar assays. The addition of TGF-beta to these cells induced erythroid differentiation which resulted in an increase in glycophorin A and a decrease in c-kit. The simultaneous addition of bFGF and TGF-beta to K562 cells prevented both the TGF-beta-mediated increase in glycophorin A expression and the decrease in c-kit expression associated with erythroid differentiation. bFGF antagonised the TGF-beta-mediated promotion of erythroid differentiation in K562 cells in a dose dependent manner and these two cytokines counteracted each other on an approximately molar basis. These results indicate that bFGF alone increases expression of c-kit and promotes a primitive phenotype in K562 cells. In addition, bFGF counteracts the effects of differentiation-inducing cytokines, such as TGF-beta, on hematopoietic cells. It is therefore possible that enhanced production of bFGF by leukemic cells could contribute to their neoplastic phenotype by opposing the effects of negative regulators or cytokines that induce differentiation. 相似文献
147.
148.
M Burger J A Burger R C Hoch Z Oades H Takamori I U Schraufstatter 《Journal of immunology (Baltimore, Md. : 1950)》1999,163(4):2017-2022
The chemokine receptor CXCR2 is the closest homologue to Kaposi's sarcoma herpesvirus-G protein-coupled receptor (KSHV-GPCR), which is known to be constitutively activated and able to cause oncogenic transformation. Among G protein-coupled receptors, a DRY sequence in the second intracellular loop is highly conserved. However, the KSHV-GPCR shows a VRY sequence instead. In this study, we exchanged Asp138 of the DRY sequence in the CXCR2 with a Val (D138V), the corresponding amino acid in KSHV-GPCR, or with a Gln (D138Q), and investigated the functional consequences of these mutations. In focus formation and soft agar growth assays in NIH 3T3 cells, the D138V mutant exhibited transforming potential similar to the KSHV-GPCR. Surprisingly, the CXCR2 wild type itself showed transforming activity, although not as potently, due to continuous autocrine stimulation, whereas the D138Q mutant formed no foci. In agreement with these results were high levels of inositol phosphate accumulation in the D138V mutant and the KSHV-GPCR, indicating constitutive activity. These data emphasize the importance of the DRY sequence for G protein-coupled signaling of the CXCR2. Either constitutive activation or persistent autocrine stimulation of the CXCR2 causes transformation similar to KSHV-GPCR-transfected cells, probably activating the same signal transduction cascade that can abrogate normal growth control mechanisms. 相似文献
149.
150.
A A Vandenbark D R Burger R M Vetto 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1975,148(4):1233-1236
Guinea pigs injected with Freund's incomplete adjuvant emulsified with guinea pig spinal cord, purified guinea pig myelin basic protein, or human myelin basic protein showed dermal reactivity to both of the basic proteins as well as to mycobacteria antigens. Animals receiving only mycobacteria antigens expressed dermal reactivity to the sensitizing antigen in addition to basic protein. This cross reactivity may help explain the role of mycobacteria in inducing and protecting against EAE, and may have important implications concerning human demyelinating diseases. 相似文献