全文获取类型
收费全文 | 246篇 |
免费 | 21篇 |
专业分类
267篇 |
出版年
2021年 | 4篇 |
2020年 | 4篇 |
2019年 | 2篇 |
2018年 | 4篇 |
2017年 | 11篇 |
2016年 | 9篇 |
2015年 | 7篇 |
2014年 | 9篇 |
2013年 | 12篇 |
2012年 | 12篇 |
2011年 | 13篇 |
2010年 | 8篇 |
2009年 | 3篇 |
2008年 | 4篇 |
2007年 | 7篇 |
2006年 | 10篇 |
2005年 | 6篇 |
2004年 | 9篇 |
2003年 | 12篇 |
2002年 | 8篇 |
2001年 | 8篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1992年 | 6篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1981年 | 2篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 7篇 |
1976年 | 5篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 6篇 |
1971年 | 7篇 |
1970年 | 3篇 |
1968年 | 2篇 |
1947年 | 1篇 |
1907年 | 1篇 |
排序方式: 共有267条查询结果,搜索用时 15 毫秒
41.
Further identification of the nature and linkage of the carbohydrate in hemoglobin A1c. 总被引:24,自引:0,他引:24
H F Bunn D N Haney K H Gabbay P M Gallop 《Biochemical and biophysical research communications》1975,67(1):103-109
We have found that Hb A1c contains neutral sugars which are only partially hydrolyzed from the N-termini of β chains. In both normal and diabetic Hb A1c, 0.2–0.3 equivalents of hexose were released, composed primarily of glucose and mannose in a 3:1 ratio. When Hb A1c was reduced with 3HNa BH4 and then treated with periodic acid, most of the radioactivity was recovered as 3H-formic acid with much lesser amounts of 3H-formaldehyde. From these results, we propose that in the red cell, glucose binds to the α-amino position of hemoglobin β-chains (valine) in an aldimine (Schiff base) linkage. This aldimine can then partially rearrange in a reversible manner to form a ketoamine linkage which is stable to acid hydrolysis. This Amadori-type rearrangement accounts for the formation of mannose, the C-2 epimer of glucose, as well as the inability to demonstrate 3HNa BH4 reduction at the C-1 position. 相似文献
42.
43.
Wayland Yeung Annie Kwon Rahil Taujale Claire Bunn Aarya Venkat Natarajan Kannan 《Molecular biology and evolution》2021,38(12):5625
The emergence of multicellularity is strongly correlated with the expansion of tyrosine kinases, a conserved family of signaling enzymes that regulates pathways essential for cell-to-cell communication. Although tyrosine kinases have been classified from several model organisms, a molecular-level understanding of tyrosine kinase evolution across all holozoans is currently lacking. Using a hierarchical sequence constraint-based classification of diverse holozoan tyrosine kinases, we construct a new phylogenetic tree that identifies two ancient clades of cytoplasmic and receptor tyrosine kinases separated by the presence of an extended insert segment in the kinase domain connecting the D and E-helices. Present in nearly all receptor tyrosine kinases, this fast-evolving insertion imparts diverse functionalities, such as post-translational modification sites and regulatory interactions. Eph and EGFR receptor tyrosine kinases are two exceptions which lack this insert, each forming an independent lineage characterized by unique functional features. We also identify common constraints shared across multiple tyrosine kinase families which warrant the designation of three new subgroups: Src module (SrcM), insulin receptor kinase-like (IRKL), and fibroblast, platelet-derived, vascular, and growth factor receptors (FPVR). Subgroup-specific constraints reflect shared autoinhibitory interactions involved in kinase conformational regulation. Conservation analyses describe how diverse tyrosine kinase signaling functions arose through the addition of family-specific motifs upon subgroup-specific features and coevolving protein domains. We propose the oldest tyrosine kinases, IRKL, SrcM, and Csk, originated from unicellular premetazoans and were coopted for complex multicellular functions. The increased frequency of oncogenic variants in more recent tyrosine kinases suggests that lineage-specific functionalities are selectively altered in human cancers. 相似文献
44.
普氏野马(Equus przewalskii)和野驴(Equus hemionus)是许家窑遗址动物群中的优势属种。本文基于对这两种动物牙齿材料的测量与分析,确定了遗址中马科动物的死亡年龄,并对上、下文化层的死亡年龄分布进行了研究,以期探知古人类获取肉食资源的方式与特点。通过与马科动物在自然生存状态下以及死于不同原因(如疾病或营养衰竭、食肉动物猎杀、现代人类狩猎等)的年龄结构对比,结果表明:古人类在许家窑文化早期(下文化层)可能通过捡拾自然死亡的动物尸体、与食肉类动物抢夺猎物、主动狩猎等多种方式获取马科动物,而在许家窑文化晚期(上文化层)可能以主动狩猎作为获取马科动物的主要方式。此外,古人类在遗址的早期就可能已经具有捕获整个马科动物居群中任意年龄个体的能力,并能做出最优化判断,有选择地去捕猎脂肪和肉量较高的壮年动物群体。 相似文献
45.
46.
47.
Bunn JM Boyer DM Lipman Y St Clair EM Jernvall J Daubechies I 《American journal of physical anthropology》2011,145(2):247-261
Inferred dietary preference is a major component of paleoecologies of extinct primates. Molar occlusal shape correlates with diet in living mammals, so teeth are a potentially useful structure from which to reconstruct diet in extinct taxa. We assess the efficacy of Dirichlet normal energy (DNE) calculated for molar tooth surfaces for reflecting diet. We evaluate DNE, which uses changes in normal vectors to characterize curvature, by directly comparing this metric to metrics previously used in dietary inference. We also test whether combining methods improves diet reconstructions. The study sample consisted of 146 lower (mandibular) second molars belonging to 24 euarchontan taxa. Five shape quantification metrics were calculated on each molar: DNE, shearing quotient, shearing ratio, relief index, and orientation patch count rotated (OPCR). Statistical analyses were completed for each variable to assess effects of taxon and diet. Discriminant function analysis was used to assess ability of combinations of variables to predict diet. Values differ significantly by diets for all variables, although shearing ratios and OPCR do not distinguish statistically between insectivores and folivores or omnivores and frugivores. Combined analyses were much more effective at predicting diet than any metric alone. Alone, relief index and DNE were most effective at predicting diet. OPCR was the least effective alone but is still valuable as the only quantitative measure of surface complexity. Of all methods considered, DNE was the least methodologically sensitive, and its effectiveness suggests it will be a valuable tool for dietary reconstruction. 相似文献
48.
Wang W Guo Y Xu M Huang HH Novikova L Larade K Jiang ZG Thayer TC Frontera JR Aires D Ding H Turk J Mathews CE Bunn HF Stehno-Bittel L Zhu H 《Biochimica et biophysica acta》2011,1812(11):1532-1541
49.
Biosynthesis of the lilac alcohols and alcohol epoxides from linalool in ‘Hortgem Tahi’ kiwifruit (Actinidiaarguta) flowers was investigated by incubating flowers with rac-linalool, rac-[4,4,10,10,10-2H5]linalool, (R)-8-hydroxylinalool and (R)-8-oxolinalool. All substrates were incorporated into the lilac alcohols although the (R)-configured compounds are not normally present in flowers. Biosynthesis of the lilac alcohol epoxides from rac-1,2-epoxy[4,4,10,10,10-2H5]linalool and rac-[4′,4′, 8′, 8′,8′-2H5]lilac aldehyde epoxide, rather than the lilac alcohols, was examined. Both substrates were non-enantioselectively converted to the lilac alcohol epoxides, suggesting two biosynthetic pathways for these compounds, contrary to previous reports. Their ability to process unnatural substrates indicates that A.arguta flowers have a greater biosynthetic capability than is suggested by their phytochemical composition. Linalool, the lilac compounds, and their biosynthetic intermediates were measured in the pistils, stamen, petals and sepals to determine if localisation in different organs contributed to only (S)-linalool being processed to the lilac compounds. Both linalool enantiomers were present in all organs, while most (97%) of the lilac compounds, and their precursors, were found in the petals. (S)-Linalool was not depleted from the flower petals, with respect to (R)-linalool, during the time of maximum production of the metabolites of (S)-linalool. 相似文献
50.
Marie-Anne Durand Lewis Carpenter Hayley Dolan Paulina Bravo Mala Mann Frances Bunn Glyn Elwyn 《PloS one》2014,9(4)