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31.
A. M. Kudryavtsev S. P. Martynov M. Broggio M. Buiatti 《Russian Journal of Genetics》2004,40(10):1102-1110
Polymorphism at 28 SSR loci was analyzed and described in 45 cultivars of spring durum wheat created in the former USSR and Russia during the last 80 years. Each cultivar was shown to have a unique allele combination. This allows SSR markers to be used to identify durum wheat varieties. Meanwhile, these markers can hardly be used to detect phylogenetic relationships among varieties and to specify their pedigrees, because genetic distances calculated on the basis of these markers do not correlate with the distance calculated by coefficient of parentage. 相似文献
32.
Mauro Durante Chiara Geri Marcello Buiatti Sergio Baroncelli Roberto Parenti Vittoria Nuti Ronchi Guido Martini Fulvio Collina Grenci Janine Grisvard Etienne Guill 《Genesis (New York, N.Y. : 2000)》1982,3(1):25-39
The possible relevance of changes in amounts of highly repetitive DNA sequences for plant differentiation and dedifferentiation processes has been suggested in several cases. Data are lacking however on (1) the genetic control of these phenomena and (2) cause-effect relationships between DNA amplification and specific ontogenetic patterns. The present study was carried out on a Nicotiana genetic system consisting of the tumorous amphidiploid N glauca X N langsdorffii, a nontumorous mutant of it, their F1, and a backcross to the tumorous parent. Backcross segregation ratios were shown to be compatible with a “single gene” hypothesis, the F1 plant being nontumorous but showing a low percentage of tumors induced by wounds, 6-azauracil or X-rays. In vitro studies of excised pith tissue grown on Linsmaier and Skoog medium for different periods of time showed the presence, confirmed by cytological analyses, of amplification of highly repetitive sequences only in the nontumorous stock, as judged by reassociation experiments in the first 24–96 hours of culture. CsCl analytical ultracentrifugation of those sequences showed the appearance in the same stock of a heavy DNA satellite (density = 1.721 gm/ml), whose presence was also confirmed by derivative melting curves. Amplification seemed to be essential for the initiation of cell division, which was completely inhibited in the nontumorous genotype and partially influenced in the F1 by incorporation during the critical period (24–96 hours of the primary explant) of 5-bromo-2′-deoxy-uridine. The results are discussed in terms of an hypothesis of an integrated gene-controlled, hormone-mediated regulatory system of cell proliferation involving changes in target repetitive DNA sequences. 相似文献
33.
Stephanie Fichtner Tilko Reents Sonia Ammar Verena Semmler Susanne Kathan Roger Dillier Alexandra Buiatti Gabriele Hessling Isabel Deisenhofer 《Indian pacing and electrophysiology journal》2015,15(3):152-157
Introduction
Pulmonary vein reconnection after pulmonary vein isolation (PVI) is a significant problem in the treatment of paroxysmal atrial fibrillation (AF). We report about patients who underwent contact force (CF) guided PVI using CF catheter and compared them to patients with PVI using an ablation catheter with enhanced tip irrigation.Methods
A total of 59 patients were included in the analysis. In 30 patients circumferential PVI was performed using the Thermocool Smarttouch® ablation catheter (ST) whereas in 29 patients circumferential PVI using the Thermocool Surround Flow SF® ablation catheter (SF) was performed. Patients were compared in regard to procedure time, fluoroscopy time/dose as well as RF-application duration and completeness of PVI. Adverse events (pericardial effusion, PV stenosis, stroke, death) were evaluated. The presence of sinus rhythm off antiarrhythmic medication was assessed during 6 months follow-up using multiple 7 day Holter-ECGs.Results
In both groups, all PVs were isolated without serious adverse events. Procedure time was 2.15 ± 0.5 h (ST) vs. 2.37 ± 1.13 h (SF) (p = 0.19). Duration of RF-applications was 46.6 ± 18 min (ST) and 49.8 ± 19 min (SF) (p = 0.52). Fluoroscopy time was 25.2 ± 13 min (ST) vs. 29 ± 18 min (SF), fluoroscopy dose 2675.6 ± 1658 versus 3038.3 ± 1997 cGym2 (p = 0.36 and 0.46 respectively). Sinus rhythm off antiarrhythmic medication validated with 7 day Holter ECGs was present in both groups in 72% of patients after 6 months of follow up.Conclusion
PVI using the new contact force catheter is safe and effective in patients with paroxysmal AF. 相似文献34.
Polymorphism at 28 SSR loci was analyzed and described in 45 cultivars of spring durum wheat created in the former USSR and Russia during the last 80 years. Each cultivar was shown to have a unique allele combination. This allows SSR markers to be used to identify durum wheat varieties. Meanwhile, these markers can hardly be used to detect phylogenetic relationships among varieties and to specify their pedigrees, because genetic distances calculated on the basis of these markers do not correlate with the distance calculated by coefficient of parentage. 相似文献
35.
With the aim of understanding better the distribution and evolution of Agrobacterium rhizogenes genes transferred in the genus Nicotiana, 42 species were screened for presence of rolB, rolC, ORF13, and ORF14. The transferred sequences were then compared within the genus and with current bacterial sequences. The results obtained showed the presence of at least one bacterial gene in 15 species belonging to different subgenera. Sequence analyses supported the hypothesis of coevolution of bacterial and plant sequences, thus suggesting a possible role for the transferred genes in the early events of Nicotiana species differentiation. The high level of conservation of Agrobacterium sequences and the dependence of their expression from the plant physiological context along with previous data suggesting their involvement in the determination of the plant hormonal balance were all consistent with this hypothesis. The results are finally discussed also as to their relevance for the hypothesis of mono and multi ancient infection by Agrobacterium. 相似文献
36.
E. Storti P. Bogani P. Bettini P. Bittini M. L. Guardiola M. G. Pellegrini D. Inzé M. Buiatti 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(1):89-96
We have studied the effect of a change in the endogenous hormone equilibria on the competence of tomato (Lycopersicon esculentum) cells to defend themselves against the fungal pathogen Fusarium oxysporum f. sp. lycopersici. Calluses from cvs Davis and Red River, respectively resistant and susceptible to Fusarium and transgenic for an auxin- or cytokinin-synthesizing gene from Agrobacterium tumefaciens, were used. The integration of Agrobacterium hormone-related genes into susceptible cv Red River can bring the activation of defense processes to a stable competence as assessed by the inhibition of mycelial growth in dual culture and gem-tube elongation of Fusarium conidia, the determination of callose contents, peroxidase induction and ion leakage in the presence of fusaric acid. This is particularly true when the transformation results in a change of phytohormone equilibria towards an higher cytokin in concentration. On the contrary, in resistant cv Davis the inhibition of both fungal growth in dual culture and conidia germination is higher when the hormone balance is modified in favour of the auxins. No significant effect was observed for ion leakage and peroxidase induction, probably because of a constitutive overproduction of cytokinins in Davis cells. 相似文献
37.
P. Bogani M. Buiatti S. Tegli M. G. Pellegrini P. Bettini A. Scala 《Plant Systematics and Evolution》1985,151(1-2):19-29
Differentiation on hormoneless media, habituation ability and crown gall induction inNicotiana tissue cultures have been used as physiological parameters of evolutionary differentiation between species. Some of them
on hormone free media differentiated whole plantlets, others produced only shoots or roots or showed undifferentiated growth
(habituation), some eventually died. Moreover, the same genotypes showed a differential behaviour as far as tumor formation
byAgrobacterium tumefaciens was concerned. Particularly, the competence for crown gall transformation inNicotiana species seems negatively correlated with differentiation capacity and may be ascribed to differences in the plants capacity
to synthesize growth regulators. The correlation between the results obtained and the phylogenetic position of the genotypes
tested is finally discussed. 相似文献
38.
A. Scala P. Bettini M. Buiatti P. Bogani G. Pellegrini F. Tognoni 《Journal of Phytopathology》1985,113(1):90-94
The behaviour of Tomato cultures from known resistant and susceptible cultivars and lines was examined for callus growth on culture media containing increasing concentrations of Fusarium oxysporum f. sp. lycopersici race 1 culture filtrate and phytoalexin synthesis elicited by Fusarium cell wall components. A strict correlation was found between in vivo resistance to the fungus and in vitro hypersensitive response and phytoalexin induction. On the other hand in vitro tolerance to toxic filtrate does not seem in this case a good indicator of in vivo resistance to the pathogen. 相似文献
39.
40.
A mathematical model of the cell movements due to cell division is presented. In the model we assume that every cell is a computational object with a given volume, and that the cell pushes the neighbouring cells in order to acquire the space for this volume. The Force that each cell exerts over the other cells is derived from a harmonic arbitrary Potential. The main parameter of the model is the average distance among the cells, that checks if the system is in spatial equilibrium or not. We show that just changing the physical constraints we can model two different systems, a two-dimensional culture on a plate and a three-dimensional early embryo. In both cases the patterns of the cell populations we obtain are similar to the real ones. 相似文献