全文获取类型
收费全文 | 4919篇 |
免费 | 474篇 |
国内免费 | 2篇 |
出版年
2023年 | 22篇 |
2022年 | 67篇 |
2021年 | 100篇 |
2020年 | 59篇 |
2019年 | 77篇 |
2018年 | 93篇 |
2017年 | 63篇 |
2016年 | 136篇 |
2015年 | 203篇 |
2014年 | 230篇 |
2013年 | 252篇 |
2012年 | 357篇 |
2011年 | 336篇 |
2010年 | 192篇 |
2009年 | 170篇 |
2008年 | 285篇 |
2007年 | 308篇 |
2006年 | 247篇 |
2005年 | 258篇 |
2004年 | 257篇 |
2003年 | 192篇 |
2002年 | 197篇 |
2001年 | 66篇 |
2000年 | 60篇 |
1999年 | 75篇 |
1998年 | 64篇 |
1997年 | 35篇 |
1996年 | 31篇 |
1995年 | 28篇 |
1994年 | 29篇 |
1992年 | 46篇 |
1991年 | 40篇 |
1990年 | 49篇 |
1989年 | 43篇 |
1988年 | 33篇 |
1987年 | 39篇 |
1986年 | 26篇 |
1985年 | 28篇 |
1984年 | 30篇 |
1983年 | 32篇 |
1982年 | 30篇 |
1981年 | 44篇 |
1980年 | 25篇 |
1979年 | 30篇 |
1978年 | 23篇 |
1977年 | 37篇 |
1976年 | 28篇 |
1975年 | 25篇 |
1974年 | 27篇 |
1973年 | 26篇 |
排序方式: 共有5395条查询结果,搜索用时 15 毫秒
11.
Localized P1 mutagenesis was used to screen for conditionally lethal mutations in ribosomal protein genes. One such mutation, 2859mis, has been mapped inside the ribosomal protein gene cluster at 72 minutes on the Escherichia coli chromosome and cotransduces at 98% with rpsE (S5). The 2869mis mutation leads to thermosensitivity and impaired assembly in vivo of 50 S ribosomal particles at 42 °C. The strain carrying the mutation has an altered L24 ribosomal protein which at 42 °C shows weaker affinity for 23 S RNA than the wild-type protein. The mutational alteration involves a replacement of glycine by aspartic acid in protein L24 from the mutant. We conclude therefore that the 2859mis mutation affects the structural gene for protein L24 (rplX). 相似文献
12.
Bryan Byrne 《American anthropologist》2000,102(2):369-370
The Material Life of Human Beings: Artifacts, Behavior, and Communication. Michael Brian Schiffer with Andrea R. Miller. New York: Routledge, 1999. 158 pp. 相似文献
13.
Novel endogenous human retroviral sequences were cloned by low-stringency hybridization, using the pol gene of endogenous human retrovirus 51-1. One clone, lambda NP-2, contained gag, pol, env, and long terminal repeat sequences related to the corresponding portions of clone 51-1 and the closely related full-length endogenous human retrovirus 4-1. The sequence of the env gene of NP-2 was 73% homologous to that of 4-1. Genomic Southern blots of male and female DNAs showed that NP-2 is located on the Y chromosome and that the Y chromosome also contains one other sequence closely related to the env and 3' flanking regions of NP-2. Conservation of flanking DNA suggests that the second Y chromosome copy of the NP-2 env sequence arose by gene duplication rather than provirus insertion. 相似文献
14.
Auto anti-A1 and auto anti-NA1 after bone marrow transplantation 总被引:1,自引:0,他引:1
P Herzog P Korínková M Stambergová M Lukásová 《Folia haematologica (Leipzig, Germany : 1928)》1987,114(6):874-880
The production of auto anti-A1 and auto anti-NA1 antibodies in patient with aplastic anemia has been described. The patient of group A1 received bone marrow from his brother of group A2. For immunosuppression cyclosporine A was administered. 相似文献
15.
Bryan D. Noe Sharon L. Milgram A. Balasubramaniam P. C. Andrews Jaroslaw Calka John K. McDonald 《Cell and tissue research》1989,257(2):303-311
Summary Results from a previous report demonstrate that more than one molecular form of neuropeptide Y-like peptide may be present in the islet organ of the anglerfish (Lophius americanus). Most of the neuropeptide Y-like immunoreactive material was anglerfish peptide YG, which is expressed in a subset of islet cells, whereas an additional neuropeptide Y-like peptide(s) was localized in islet nerves. To learn more about the neuropeptide Y-like peptides in islet nerves, we have employed immunohistochemical and biochemical methods to compare peptides found in anglerfish islets and brain. Using antisera that selectively react with either mammalian forms of neuropeptide Y or with anglerfish peptide YG, subsets of neurons were found in the brain that labelled with only one or the other of the antisera. In separate sections, other neurons that were labelled with either antiserum exhibited similar morphologies. Peptides from brains and islets were subjected to gel filtration and reverse-phase high performance liquid chromatography. Radioimmunoassays employing either the neuropeptide Y or peptide YG antisera were used to examine chromatographic eluates. Immunoreactive peptides having retention times of human neuropeptide Y and porcine neuropeptide Y were identified in extracts of both brain and islets. This indicates that peptides structurally similar to both of these peptides from the neuropeptide Y-pancreatic polypeptide family are expressed in neurons of anglerfish brain and nerve fibers of anglerfish islets. The predominant form of neuropeptide Y-like peptide in islets was anglerfish peptide YG. Neuropeptide Y-immunoreactive peptides from islet extracts that had chromatographic retention times identical to human neuropeptide Y and porcine neuropeptide Y were present in much smaller quantities. These results are consistent with the hypothesis that peptides having significant sequence homology with human neuropeptide Y and porcine neuropeptide Y are present in the nerve fibers that permeate the islet. 相似文献
16.
Porcine D-amino acid oxidase: production of the biologically active enzyme in Escherichia coli 总被引:4,自引:0,他引:4
E Ciccarelli M Massaer J P Guillaume A Herzog R Loriau A Cravador P Jacobs A Bollen 《Biochemical and biophysical research communications》1989,161(2):865-872
DNA molecules coding either for mature porcine D-amino acid oxidase or for truncated forms of the enzyme have been obtained by stepwise addition of synthetic oligonucleotides to a partial cDNA. Under the control of the lambda PL thermoregulatable promoter, these DNAs were respectively expressed in Escherichia coli as 36, 28 and 25 kilodalton polypeptides, specifically recognised by antibodies raised against the natural enzyme. None of the truncated proteins were biologically active whereas the mature recombinant species was able to hydrolyze D-alanine in vitro as efficiently as the natural product. 相似文献
17.
Summary Phosphinothricin is a non-selective herbicide which inhibits glutamine synthetase (EC 6.3.1.2) activity causing an overaccumulation of ammonia in higher plants. Alfalfa (Medicago sativa L) shoot tissue and petiole-derived callus exposed to phosphinothricin show 50 and 70% reductions, respectively, in glutamine synthetase activity with a concomitant rise of 10 and 20 fold, respectively, in endogenous ammonia. The diffusibility of ammonia may limit the use of a detoxifying gene, phosphinothricin acetyltransferase, as a selectable marker for alfalfa transformation. However, the addition of up to 40 times the standard levels of ammonium nitrate to the culture media used in this study had no effect on callus growth, although glutamine synthetase activity was inhibited by 50% and endogenous ammonia increased 27 fold. Therefore, ammonia accumulation may not be the primary cause of cell death in alfalfa after exposure to phosphinothricin. It follows that diffusion of ammonia from cell to cell would not restrict the selection for phosphinothricin acetyltransferase transformed cells, thereby indicating that this enzyme could be used as a selectable marker in transformation experiments.Abbreviations PPT
Phosphinothricin
- PAT
Phosphinothricin acetyltransferase 相似文献
18.
Homozygous osteogenesis imperfecta unlinked to collagen I genes 总被引:4,自引:1,他引:3
Katherine Aitchison Donald Ogilvie Mary Honeyman Elizabeth Thompson Bryan Sykes 《Human genetics》1988,78(3):233-236
Summary In a consanguineous pedigree in which a severe type of osteogenesis imperfecta was segregating as an autosomal recessive trait, analysis of genetic markers for both collagen I structural loci COL1A1 and COL1A2 showed that the phenotype was unlinked to either locus. 相似文献
19.
J Bryan 《The Journal of cell biology》1988,106(5):1553-1562
Gelsolin, a Ca2+-modulated actin filament-capping and -severing protein, complexes with two actin monomers. Studies designed to localize binding sites on proteolytic fragments identify three distinct actin-binding peptides. 14NT, a 14-kD fragment that contains the NH2 terminal, will depolymerize F-actin. This peptide forms a 1:1 complex with G-actin which blocks the exchange of etheno-ATP from bound actin. The estimated association and dissociation rates for this complex are 0.3 microM-1 s-1 and 1.35 x 10(-6) s-1 which gives a maximum calculated Kd = 4.5 x 10(-12) M. 26NT, the adjacent peptide on the NH2-terminal half of gelsolin, binds to both G- and F-actin. This fragment has little or no intrinsic severing activity and will bind to F-actin to nearly stoichiometric ratios. The interactions of 14NT and 26NT with actin are largely Ca2+ independent and one of these sites, probably 14NT, is the EGTA-stable site identified in the intact protein. 41CT, the COOH-terminal half of gelsolin, forms a rapidly reversible 1:1 complex with actin, Kd = 25 nM, that slows but does not block etheno-ATP exchange. This interaction is Ca2+ dependent and is the exchangeable site in the intact protein. One of these sites is hidden in the intact protein, but cleavage into half fragments exposes all three and removes the Ca2+ dependence of severing. 相似文献
20.
Comparison of diaphragmatic fatigue in newborn and older rabbits 总被引:1,自引:0,他引:1
Le Souef P. N.; England S. J.; Stogryn H. A.; Bryan A. C. 《Journal of applied physiology》1988,65(3):1040-1044
The ability to maintain occlusion pressure (i.e., fatigability) during activation of the diaphragm via phrenic nerve stimulation was compared in newborn (less than 14 days old) and older (greater than 30 days old) rabbits. The younger animals had lower maximum inspiratory pressures (MIP) and markedly greater falls in pressure during sustained diaphragmatic contractions at greater than 40% MIP than did the older animals. Histological analysis showed a paucity of high-oxidative type I fibers in the diaphragms of the young animals. We therefore conclude that the newborn rabbit diaphragm is extremely susceptible to fatigue and that this susceptibility correlates with the distribution of muscle fiber types. 相似文献