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971.
Ant queens mate when young and store sperm in their spermatheca to fertilize eggs for several years until their death. In contrast, workers in most species never mate. We have compared the histological organization of spermathecae in 25 poneromorph species exhibiting various degrees of queen-worker dimorphism. The spermathecae of both castes in all species are similar in having a reservoir connected by a sperm duct to the ovary, and a paired gland opening into this duct. The reservoir of queens typically has a columnar epithelium in the hilar region (near the opening of the sperm duct), whereas the epithelium in the distal region is cuboidal. Abundant mitochondria together with apical microvilli and basal invaginations indicate an osmoregulatory function. In contrast, the reservoir epithelium of workers is flattened throughout and lacks these transport characteristics. This single difference shows the importance of a columnar epithelium in the reservoir for sperm storage. However, our data have not revealed inter-specific variations in the development of the hilar region linked with higher fecundity. We have found no consistent differences in associated structures, such as the spermatheca gland or sperm ducts, or in the musculature between queens and workers.This work was funded by IWT, FWO, KULeuven OT and JSPS. 相似文献
972.
973.
Manival X Charron C Fourmann JB Godard F Charpentier B Branlant C 《Nucleic acids research》2006,34(3):826-839
In archaeal rRNAs, the isomerization of uridine into pseudouridine (Ψ) is achieved by the H/ACA sRNPs and the minimal set of proteins required for RNA:Ψ-synthase activity is the aCBF5–aNOP10 protein pair. The crystal structure of the aCBF5–aNOP10 heterodimer from Pyrococcus abyssi was solved at 2.1 Å resolution. In this structure, protein aNOP10 has an extended shape, with a zinc-binding motif at the N-terminus and an α-helix at the C-terminus. Both motifs contact the aCBF5 catalytic domain. Although less efficiently as does the full-length aNOP10, the aNOP10 C-terminal domain binds aCBF5 and stimulates the RNA-guided activity. We show that the C-terminal domain of aCBF5 (the PUA domain), which is wrapped by an N-terminal extension of aCBF5, plays a crucial role for aCBF5 binding to the guide sRNA. Addition of this domain in trans partially complement particles assembled with an aCBF5ΔPUA truncated protein. In the crystal structure, the aCBF5–aNOP10 complex forms two kinds of heterotetramers with parallel and perpendicular orientations of the aNOP10 terminal α-helices, respectively. By gel filtration assay, we showed that aNOP10 can dimerize in solution. As both residues Y41 and L48 were needed for dimerization, the dimerization likely takes place by interaction of parallel α-helices. 相似文献
974.
Tost J Jammes H Dupont JM Buffat C Robert B Mignot TM Mondon F Carbonne B Siméoni U Grangé G Kerjean A Ferré F Gut IG Vaiman D 《Nucleic acids research》2006,34(19):5438-5448
Expression of imprinted genes is classically associated with differential methylation of specific CpG-rich DNA regions (DMRs). The H19/IGF2 locus is considered a paradigm for epigenetic regulation. In mice, as in humans, the essential H19 DMR--target of the CTCF insulator--is located between the two genes. Here, we performed a pyrosequencing-based quantitative analysis of its CpG methylation in normal human tissues. The quantitative analysis of the methylation level in the H19 DMR revealed three unexpected discrete, individual-specific methylation states. This epigenetic polymorphism was confined to the sixth CTCF binding site while a unique median-methylated profile was found at the third CTCF binding site as well as in the H19 promoter. Monoallelic expression of H19 and IGF2 was maintained independently of the methylation status at the sixth CTCF binding site and the IGF2 DMR2 displayed a median-methylated profile in all individuals and tissues analyzed. Interestingly, the methylation profile was genetically transmitted. Transgenerational inheritance of the H19 methylation profile was compatible with a simple model involving one gene with three alleles. The existence of three individual-specific epigenotypes in the H19 DMR in a non-pathological situation means it is important to reconsider the diagnostic value and functional importance of the sixth CTCF binding site. 相似文献
975.
Partitioning the effects of algal species identity and richness on benthic marine primary production 总被引:6,自引:1,他引:5
John F. Bruno Sarah C. Lee Johanna S. Kertesz Robert C. Carpenter Zachary T. Long J. Emmett Duffy 《Oikos》2006,115(1):170-178
Influential research in terrestrial habitats indicates that several ecosystem processes are related to plant biodiversity, yet these links remain poorly studied in marine ecosystems. We conducted one field and one mesocosm experiment to quantify the relative effects of macroalgal species identity and richness on primary production in coral reef macroalgal communities off the north coast of Jamaica. We measured production as the net accumulation of algal biomass in the absence of consumers and as photosynthetic rate using oxygen probes in sealed aquaria. We used two recently developed techniques to attribute deviations in expected relative yield to components associated with species identity or diversity and then to further partition diversity effects into mechanistic components based on dominance, trait-dependent complementarity, and trait-independent complementarity. Our results indicate that algal identity had far greater effects on absolute net growth and photosynthesis than richness. The most diverse mixture of macroalgae did not outperform the most productive monoculture or the average monoculture in either measure of primary production (i.e. we did not find evidence of either transgressive or non-transgressive overyielding). Trait-independent complementarity effects were positive but dominance and trait-dependent complementarity were both negative and became stronger when richness was increased. Thus the potentially positive influence of species interactions and niche partitioning on production were negated by dominance and other negative selection effects. These results demonstrate that the counteracting influence of component effects can diminish the net richness effects on production. This could explain frequently observed weak net richness effects in other aquatic and terrestrial systems and suggests that life history tradeoffs greatly reduce the potential for ecologically relevant plant biodiversity effects on ecosystem properties. 相似文献
976.
Marco Caccianiga Alessandra Luzzaro Simon Pierce Roberta M. Ceriani Bruno Cerabolini 《Oikos》2006,112(1):10-20
CSR classification aims to apply CSR theory to large numbers of plants in situ, thereby allowing the investigation of communities within a functional context. However, it has only ever been applied to British vegetation, during the development of the technique, and has not yet been used to investigate specific vegetation processes. Here, a vegetation primary succession on a glacier foreland (Rutor glacier, Aosta, Italy) was used as a 'test bed' for the hypothesis that CSR classification can distinguish functional shifts during this vegetation process. Morpho-functional traits were used to calculate CSR coordinates for 45 species throughout the glacier foreland. General functional similarities between species were verified using principal components analysis (PCA). CSR classification demonstrated a functional shift from broadly ruderal pioneers towards stress-tolerance in late succession. PCA 1 correlated with S and R strategies, confirming this gradient. Till deposited at the retreating glacier terminus provides a substrate that can support faster growing species (with high foliar N contents), but is only tenable to those that can avoid physical disturbance via rapid phenological development (i.e. ruderals). Stress-tolerance and lower N contents in late succession suggest selection for efficient nutrient use. CSR classification demonstrated that competitive traits were ubiquitous but of much lesser importance than stress-tolerance or ruderalism (also correlating with PCA 2 and 3). The detailed visualization provided by CSR classification, combined with its mechanistic explanation of community change, demonstrate the promise of this methodology as a quantitative tool for comparative community ecology. 相似文献
977.
Chiappetta A Michelotti V Fambrini M Bruno L Salvini M Petrarulo M Azmi A Van Onckelen H Pugliesi C Bitonti MB 《Planta》2006,223(5):917-931
978.
Mantelin S Desbrosses G Larcher M Tranbarger TJ Cleyet-Marel JC Touraine B 《Planta》2006,223(3):591-603
Both root architecture and plant N nutrition are altered by inoculation with the plant growth-promoting rhizobacteria (PGPR)
Phyllobacterium strain STM196. It is known that NO3− and N metabolites can act as regulatory signals on root development and N transporters. In this study, we investigate the
possible interrelated effects on root development and N transport. We show that the inhibition of Arabidopsis lateral root growth by high external NO3− is overridden by Phyllobacterium inoculation. However, the leaf NO3− pool remained unchanged in inoculated plants. By contrast, the Gln root pool was reduced in inoculated plants. Unexpectedly,
NO3− influx and the expression levels of AtNRT1.1 and AtNRT2.1 genes coding for root NO3− transporters were also decreased after 8 days of Phyllobacterium inoculation. Although the mechanisms by which PGPR exert their positive effects remain unknown, our data show that they can
optimize plant development independently from N supply, thus alleviating the regulatory mechanisms that operate in axenic
conditions. In addition, we found that Phyllobacterium sp. elicited a very strong induction of AtNRT2.5 and AtNRT2.6, both genes preferentially expressed in the shoots whose functions are unknown. 相似文献
979.
Alexander S. de Resende Rogério P. Xavier Octávio C. de Oliveira Segundo Urquiaga Bruno J. R. Alves Robert M. Boddey 《Plant and Soil》2006,281(1-2):339-351
Since the 1970s the area under sugarcane in Brazil has increased from 2 million to over 5 million ha (M ha), and it is expected
to pass the 7 M ha mark in 2007. More than half of the cane is harvested to produce bioethanol as a fuel for light vehicles.
The distilleries produce approximately 13 L of distillery waste (vinasse) for each litre of ethanol produced. In the 1980s
there was considerable concern over the long-term effects of the disposal of this material (containing about 1% carbon and
high in K) on cane yields if it was applied to the field. At the same time there was a growing movement to abandon the practice
of pre-harvest burning and some research was showing that some Brazilian varieties of sugar cane were able to obtain significant
contributions of N from plant-associated biological nitrogen fixation (BNF). For these reasons an experiment was installed
on a cane plantation in the state of Pernambuco, NE Brazil to investigate the long-term effects of vinasse and N fertiliser
additions and the practice of pre-harvest burning on crop and sugar yield, soil fertility parameters, N balance and soil C
stocks. The results showed that over a 16-year period, trash conservation (abandonment of burning) increased cane yields by
25% from a mean of 46 to 58 Mg ha−1. Vinasse applications (80 m3 ha−1 crop−1) increased mean cane and sugar yield by 12 to 13% and the application of 80 kg N ha−1 as urea increased cane yields by 9%, but total sugar yield by less than 6% (from 7.0 to 7.4 Mg ha−1 crop−1). The total N balance for the soil/plant system when only the surface 20 cm of the soil was considered was positive in plots
where no N fertiliser was added. However, the data indicated that during the 16 years of the study considerable quantities
of soil organic matter were accumulated below 20 cm depth such that the N balance considering the soil to 60 cm depth was
strongly positive, except where N fertiliser was added. The data indicated that there were considerable BNF inputs to the
system, which was consistent with its low response to N fertiliser and low N fertiliser-use-efficiency. There were no significant
effects of vinasse or urea addition, or trash conservation on soil C stocks, although the higher yields proportioned by trash
conservation had potentially significant benefits for increased mitigation of CO2 emissions where the main use of the cane was for bioethanol production. 相似文献
980.
Mariac C Luong V Kapran I Mamadou A Sagnard F Deu M Chantereau J Gerard B Ndjeunga J Bezançon G Pham JL Vigouroux Y 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,114(1):49-58
Genetic diversity of crop species in sub-Sahelian Africa is still poorly documented. Among such crops, pearl millet is one of the most important staple species. In Niger, pearl millet covers more than 65% of the total cultivated area. Analyzing pearl millet genetic diversity, its origin and its dynamics is important for in situ and ex situ germplasm conservation and to increase knowledge useful for breeding programs. We developed new genetic markers and a high-throughput technique for the genetic analysis of pearl millet. Using 25 microsatellite markers, we analyzed genetic diversity in 46 wild and 421 cultivated accessions of pearl millet in Niger. We showed a significantly lower number of alleles and lower gene diversity in cultivated pearl millet accessions than in wild accessions. This result contrasts with a previous study using iso-enzyme markers showing similar genetic diversity between cultivated and wild pearl millet populations. We found a strong differentiation between the cultivated and wild groups in Niger. Analyses of introgressions between cultivated and wild accessions showed modest but statistically supported evidence of introgressions. Wild accessions in the central region of Niger showed introgressions of cultivated alleles. Accessions of cultivated pearl millet showed introgressions of wild alleles in the western, central, and eastern parts of Niger.Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users.Cedric Mariac and Viviane Luong have contributed equally to this work. 相似文献