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21.
C Fabris D Basso L Benini T Meggiato G Del Favero G Cavallini M P Panozzo P Fogar C Angonese I Vantini 《Enzyme》1989,42(2):80-86
Serum and urine elastase 1, its renal output and clearance and urinary gamma-glutamyltransferase and ribonuclease excretions were measured in 16 patients with pancreatic cancer, 23 with chronic pancreatitis and in 22 healthy controls in order to evaluate elastase 1 plasma-urine transfer in chronic pancreatic disease and to investigate any factors that might influence the clearance of this enzyme. In an additional group of 17 patients with different pancreatic diseases the serum molecular size distribution of elastase 1 after chromatography was ascertained. An increased urinary elastase 1 output was found in 4/16 patients with pancreatic cancer and in 6/23 with chronic pancreatitis. No correlation was found between circulating elastase 1 and its urinary output; a negative correlation was detected between the serum levels of this enzyme and its clearance. The excretion of ribonuclease and gamma-glutamyltransferase was correlated with elastase 1 output and clearance. While the majority of elastase 1 in serum was accounted for by high molecular forms, probably the expression of complexes with serum inhibitors, free circulating enzyme was present in all patients with high serum elastase 1. Our findings suggest that elastase 1 urinary excretion increases in some patients with chronic pancreatic disease regardless of the neoplastic or inflammatory nature of the illness. Although the availability of different amounts of ultrafiltrable enzyme may play a role in influencing elastase 1 plasma-urine transfer, renal tubular damage appears to be the most important factor influencing the increase in the urinary output of elastase 1. 相似文献
22.
M A Francesconi A Donella-Deana V Furlanetto L Cavallini P Palatini R Deana 《Biochimica et biophysica acta》1989,999(2):163-170
Succinyl-CoA:3-hydroxy-3-methylglutarate coenzyme A transferase, previously identified in rat-liver mitochondria (Deana et al. (1981), Biochim. Biophys. Acta 662, 119-124), was purified to near homogeneity and further characterized. After the last purification steps consisting of Ultrogel AcA-44 filtration and agarose-hexane-coenzyme A chromatography, the enzyme was apparently tetrameric with a mass of 48-52 kDa determined by gel filtration on Sephadex G-75, ultracentrifugation through a sucrose gradient and SDS-gel electrophoresis. By means of a HPLC technique developed for measuring the CoA esters we could determine the enzyme activity in both forward and reverse directions and show that the kinetic constants, i.e., Km of reactants and Vmax, are not too different for the two reactions. Double-reciprocal plots of the enzyme velocities versus the concentration of one substrate at different fixed concentrations of the other substrate gave families of straight lines converging below the substrate-abscissa for both forward and backward reactions, indicating a kinetic mechanism of rapid equilibrium random Bi-Bi type. The competitive inhibition of the product succinate with respect to both reactants, 3-hydroxy-3-methylglutarate and succinyl-CoA, as well as the Haldane relationships are consistent with this conclusion. An inhibitory effect on CoA transferase activity by acetate, acetoacetate, acetyl-CoA, acetoacetyl-CoA, coenzyme A, carnitine, ZnCl2 and high concentrations of the monovalent anions ClO4-, F-, I- and Cl- was also found. 相似文献
23.
On choice of substrate and habitat in brachionid rotifers 总被引:9,自引:9,他引:0
Information on the distribution of 28 rotifers of the family Brachionidae from diverse waters in south and central Sweden was analyzed to reveal their relationships to substrate and habitat. Some brachionids are preferably planktic, others periphytic and/or benthic. Some non-planktic habitats are utilized more than others, but there is no evidence of a chemical attraction from any substrate. Instead, some substrates seem to be avoided, possibly depending on a poorer flora of periphytic algae. Besides substrate type, the following factors are found to be important for creating separate ecological niches in the brachionid family: temperature, oxygen content, trophic degree, chemical environment, food choice and sensitivity to predation. It is possible to delineate separate ecological niches for all brachionid rotifers, implying that Hutchinson's ideas about the plankton paradox are contradicted. Some species are specialists, other are generalists, the latter being characterized by a great morphological variation. The species are adapted in different ways to their preferential habitats, as regards foot, egg-carrying, protrusions and other lorical structures etc. Longer spines, for instance, are generally found in more transparent water, being a supposed protection against visual predators. 相似文献
24.
Bruno Schwarzkopf Brigitte Reuke Andreas Kiener Adelbert Bacher 《Archives of microbiology》1990,153(3):259-263
Growing cultures of Methanobacterium thermoautotrophicum were supplemented with [U-14C]adenosine or [1-14C]adenosine. 7,8-Didemethyl-8-hydroxy-5-deazariboflavin (factor F0) and 7-methylpterin were isolated from the culture medium. Hydrolysis of cellular RNA yielded purine and pyrimidine nucleotides. The ribose side chain of proffered adenosine is efficiently incorporated into cellular adenosine and guanosine nucleotide pools but not into pyrimidine nucleotides. Thus, M. thermoautotrophicum can utilize exogenous adenosine by direct phosphorylation without hydrolysis of the glycosidic bond, and AMP can be efficiently converted to GMP. Factor F0 and 7-methylpterin had approximately the same specific activities as the purine nucleotides. It follows that the ribityl side chain of factor F0 is derived from the ribose side chain of a nucleotide precursor by reduction. The pyrazine ring of methanopterin is formed by ring expansion involving the ribose side chain of the precursor, GTP.Abbreviations Factor F0
8-hydroxy-6,7-didemethyl-5-deazariboflavin
- APRT
adenine phosphoribosyltransferase
- GPRT
guanine phosphoribosyltransferase
- PRPP
phosphoribosylpyrophosphate
- HPLC
high performance liquid chromatography 相似文献
25.
Structure and function of hemoglobin in antarctic fishes and evolutionary implications 总被引:2,自引:0,他引:2
Guido di Prisco Rossana D'Avino Laura Camardella Carla Caruso Mario Romano Bruno Rutigliano 《Polar Biology》1990,10(4):269-274
Summary The hematological features of cold-adapted, red-blooded Antarctic teleosts has prompted this study on the relationship between hemoglobin molecular structure and oxygen-binding properties. The hemolysates from 21 species of 5 families contained one component (Hb 1), often accompanied by an additional, minor one (Hb 2, 5%–10% of total). On the other hand, 3 species of Zoarcidae, a non-endemic family, had 4–5 components. All purified hemoglobins from the former group, but only 1–2 of the 4–5 hemoglobins of Zoarcidae, showed a strong Root effect (pH regulation of oxygen binding). Globins from each hemoglobin have been purified and characterised with respect to molecular structure in several species. The similarity between the complete amino acid sequence of one -chain and those of non-Antarctic -chains is lower than that among the latter sequences, suggesting independent pathways of evolution.Presented at the 5th SCAR Symposium on Antarctic Biology, Hobart, Australia (August 29th-September 3rd, 1988) 相似文献
26.
Bruno W. S. Sobral Rhonda J. Honeycutt Alan G. Atherly Michael McClelland 《Plant Molecular Biology Reporter》1990,8(4):253-275
TheOryza sativa (rice) genome is small (600 to 900 megabase pairs) when compared to that of other monocotyledonous plants. Rice was the first
of the major cereals to be successfully transformed and regenerated. An RFLP map with approximately 300 markers is readily
available, and the DNA content per map unit is only two to three times that ofArabidopsis thaliana. Rice is also the main staple food for the majority of peoples in the world. We developed techniques for the preparation
of intact genomic DNA from Indica and Japonica subspecies of rice, used statistical methods to determine which restriction
endonucleases are rare-cutting, and used pulsed-field gel electrophoresis (PFE) to separate large fragments of rice DNA. Southern
hybridization to blotted rice PFE gels was used to show that the digests were complete. The long-term goal of our work is
to generate an integrated genetic/physical map for the rice genome, as well as helping to establish rice as a model for map-based
gene cloning and genome analysis. 相似文献
27.
1. The vasorelaxant effect of synthetic atrial natriuretic peptide (ANP) on the vascular response to angiotensin II (A II) and norepinephrine (NE) in aortic rings from Bufo arenarum toad was studied. 2. Pretreatment with ANP partially inhibited the vascular response to A II and NE. 3. Angiotensin converting enzyme inhibitor (ACEI) treatment partially inhibited the contractile response of angiotensin I (A I) and did not affect the A II response. 4. The inhibitory effect of ANP on vascular response to A II and NE were potentiated by pretreatment with ACEI. 5. Results suggest that the angiotensin converting enzyme present in the vascular wall from Bufo arenarum toad may be involved in the metabolism of ANP. 相似文献
28.
In a new case of interstitial del(2q), measurements of ribulose 5-phosphate 3-epimerase activity suggested that the locus for this enzyme might be localized to the subregion 2q32q33.3. 相似文献
29.
Abstract Nif− mutants of Rhodobacter capsulatus carrying mutations either in the nifR4 regulatory gene or in the nifH structural gene both outgrew the wild-type strain B10 in mixed chemostat cultures under conditions favouring nitrogenase-mediated H2 production by the wild-type (ammonia as limiting nutrient, inert argon atmosphere, light as energy source), whereas under aerobic conditions in the dark, or in batch culture, the growth of Nif− mutants was not favoured. Nitrogenase-mediated H2 production therefore appears to be detrimental to the growth of R. capsulatus in nitrogen-limited continuous culture, as may also be the case for other nitrogen fixers. 相似文献
30.
D.W. Bruno 《FEMS microbiology letters》1988,51(2-3):135-139
Abstract The cell surface hydrophobicity of Renibacterium salmoninarum strains was examined using a salt aggregation method. Those strains which were virulent in the test animal were sticky, auto-agglutinating and possessed a hydrophobic cell surface. Those strains with a low virulence were non-sticky, non-agglutinating and failed to aggregate in a high molar salt. Strains could not be distinguished using biochemical tests. There was no change in hydrophobicity following re-isolation of the bacteria from experimentally infected rainbow trout, Salmo gairdneri . 相似文献