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621.
622.
Manuela da Silva Solc Maiara Reis Arruda Bruna Martins Macedo Leite Tiago Feitosa Mota Miriam Flores Rebouas Matheus Silva de Jesus Leila Denise Alves Ferreira Amorim Valria Matos Borges Jesus Valenzuela Shaden Kamhawi Patrícia Sampaio Tavares Veras Deborah Bittencourt Moth Fraga Claudia Ida Brodskyn 《PLoS neglected tropical diseases》2021,15(2)
BackgroundReports have shown correlations between the immune response to vector saliva and Leishmaniasis outcome. We followed dogs in an endemic area for two years characterizing resistance or susceptibility to canine visceral leishmaniasis (CVL) according to Leishmania infantum diagnosis and clinical development criteria. Then, we aimed to identify a biosignature based on parasite load, serum biological mediators’ interactions, and vector exposure intensity associated with CVL resistance and susceptibility.Methodology/Principal findingsA prospective two-year study was conducted in an area endemic for CVL. Dogs were evaluated at 6-month intervals to determine infection, clinical manifestations, immune profile, and sandfly exposure. CVL resistance or susceptibility was determined upon the conclusion of the study. After two years, 78% of the dogs were infected with L. infantum (53% susceptible and 47% resistant to CVL). Susceptible dogs presented higher splenic parasite load as well as persistence of the parasite during the follow-up, compared to resistant ones. Susceptible dogs also displayed a higher number of correlations among the investigated biological mediators, before and after infection diagnosis. At baseline, anti-saliva antibodies, indicative of exposure to the vector, were detected in 62% of the dogs, reaching 100% in one year. Higher sandfly exposure increased the risk of susceptibility to CVL by 1.6 times (CI: 1.11–2.41). We identified a discriminatory biosignature between the resistant and susceptible dogs assessing splenic parasite load, interaction of biological mediators, PGE2 serum levels and intensity of exposure to sandfly. All these parameters were elevated in susceptible dogs compared to resistant animals.Conclusions/SignificanceThe biosignature identified in our study reinforces the idea that CVL is a complex multifactorial disease that is affected by a set of factors which are correlated and, for a better understanding of CVL, should not be evaluated in an isolated way. 相似文献
623.
Cristiana Araújo Gontijo Bruna Borges Macedo Cabral Laura Cristina Tibiletti Balieiro Gabriela Pereira Teixeira Walid Makin Fahmy Yara Cristina de Paiva Maia 《Chronobiology international》2019,36(1):75-84
Animal studies strongly suggest that timed feeding can have beneficial physiological effects, including protection against the obesogenic and metabolic consequences of a high-fat diet. However, the relationship between variables related to the timing of eating and diet quality in pregnancy women, which is considered as a period of nutritional vulnerability, is still poorly described in the literature. Therefore, the aim of the present study was to investigate the associations between time-related eating patterns and chronotype with diet quality of pregnant women. This cross-sectional study was conducted with 100 pregnant women in the first gestational trimester (≤12 weeks of gestation). The information regarding food intake was obtained by three 24-Hour Dietary Recall (24HR). Time-related eating patterns, i.e., the interval between the first and the last meal (eating duration), nightly fasting, time of the first and last meals, and number of meals eating on a day were determined. Chronotype was derived using the mid-sleep time on free days on weekends, with a further correction for calculated sleep debt. Diet quality was evaluated using the Brazilian Healthy Eating Index-Revised (BHEI-R), validated for the Brazilian population. Linear regression modeling analyses adjusted for confounders were used to investigate the association between time-related eating patterns and chronotype with diet quality. The BHEI-R total score was negatively associated with time of the first meal (β = ?0.355; p = 0.002; r2 adjusted = 0.141), and positively associated with eating duration (β = 0.262; p = 0.024; r2 adjusted = 0.086) and number of meals (β = 0.273; p = 0.019; r2 adjusted = 0.091). In addition, the score of total fruit component was negatively associated with chronotype (β = ?0.236; p = 0.033; r2 adjusted = 0.078), time of the first meal (β = ?0.393; p = 0.001; r2 adjusted = 0.171), and positively associated with eating duration (β = 0.259; p = 0.022; r2 adjusted = 0.087) and number of meals (β = 0.376; p = 0.001; r2 adjusted = 0.159). The score for whole fruit component was negatively associated with time of the first meal (β = ?0.388; p = 0.001; r2 adjusted = 0.152), and positively associated with number of meals (β = 0.403; p = 0.001; r2 adjusted = 0.164). A longer eating duration, earlier time of the first meal, higher number of meals and morningness tendency are associated with a better diet quality in the first gestational trimester – higher scores of the total BHEI-R and/or fruit components. We suggest that nutritional guidelines should consider time-related eating patterns and chronotype to ensure good diet quality of pregnant women since the beginning of gestation, contributing on prevention of metabolic-nutritional complications. 相似文献
624.
Carla Vignaroli Laura Di Sante Gloria Magi Gian Marco Luna Andrea Di Cesare Sonia Pasquaroli Bruna Facinelli Francesca Biavasco 《The ISME journal》2015,9(2):508-515
Five distinct cryptic lineages (clades I–V) have recently been recognized in the Escherichia genus. The five clades encompass strains that are phenotypically and taxonomically indistinguishable from Escherichia coli sensu stricto; however, scant data are available on their ecology, virulence and pathogenic properties. In this study 20 cryptic E. coli strains isolated from marine sediments were investigated to gain insights into their virulence characteristics and genetic traits. The ability to adhere to intestinal cells was highest among clade V strains, which also harbored the genes involved in gut colonization as well as the genes (pduC and eut operon) typically found in environmentally adapted E. coli strains. The pduC gene was significantly associated with clade V. Multilocus sequence typing of three representative clade V isolates revealed new sequence types (STs) and showed that the strains shared two allelic loci (adk 51 and recA 37). Our findings suggest that cryptic Escherichia lineages are common in coastal marine sediments and that this habitat may be suitable for their growth and persistence outside the host. On the other hand, detection in clade V strains of a gene repertoire and adhesion properties similar to those of intestinal pathogenic strains could indicate their potential virulence. It could be argued that there is a dual nature of cryptic clade V strains, where the ability to survive and persist in a secondary habitat does not involve the loss of the host-associated lifestyle. Clade V could be a group of closely related, environmentally adapted E. coli strains. 相似文献
625.
Rogers Ribeiro Ivana Ribeiro de Nardi Bruna Soares Fernandes Eugenio Foresti Marcelo Zaiat 《Biodegradation》2013,24(2):269-278
Because benzene, toluene, ethylbenzene, and xylenes (BTEX) and ethanol are important contaminants present in Brazilian gasoline, it is essential to develop technology that can be used in the bioremediation of gasoline-contaminated aquifers. This paper evaluates the performance of a horizontal-flow anaerobic immobilized biomass (HAIB) reactor fed with water containing gasoline constituents under denitrifying conditions. Two HAIB reactors filled with polyurethane foam matrices (5 mm cubes, 23 kg/m3 density and 95 % porosity) for biomass attachment were assayed. The reactor fed with synthetic substrate containing protein, carbohydrates, sodium bicarbonate and BTEX solution in ethanol, at an Hydraulic retention time (HRT) of 13.5 h, presented hydrocarbon removal efficiencies of 99 % at the following initial concentrations: benzene 6.7 mg/L, toluene 4.9 mg/L, m-xylene and p-xylene 7.2 mg/L, ethylbenzene 3.7 mg/L, and nitrate 60 mg N/L. The HAIB reactor fed with gasoline-contaminated water at an HRT of 20 h showed hydrocarbon removal efficiencies of 96 % at the following initial concentrations: benzene, 4.9 mg/L; toluene, 7.2 mg/L; m-xylene, 3.7 mg/L; and nitrate 400 mg N/L. Microbiological observations along the length of the HAIB reactor fed with gasoline-contaminated water confirmed that in the first segment of the reactor, denitrifying metabolism predominated, whereas from the first sampling port on, the metabolism observed was predominantly methanogenic. 相似文献
626.
Umeed Rebecca Lucchini Karen Coutinho Paula Djanira Fernandes dos Santos Paulo Jorge Parreira Borges João Carlos Gomes Normade Iran Attademo Fernanda Löffler Niemer Luna Fabia Bezerra Bruna 《Journal of Ethology》2023,41(3):243-251
Journal of Ethology - Acoustic signalling plays a crucial role in mother–calf communication. Here, we describe the acoustic signalling of three free-living Antillean manatee mother and calf... 相似文献
627.
Pereira Bruna M. Guimaraes Larissa A. Souza Nara O. S. Saraiva Mario A. P. Guimaraes Patricia M. Brasileiro Ana C. M. 《Plant Molecular Biology Reporter》2019,37(1-2):74-86
Plant Molecular Biology Reporter - Plant parasitic root-knot nematodes (RKN) have evolved sophisticated strategies for exploiting plants, and they cause devastating yield losses in many susceptible... 相似文献
628.
629.
Filho Robério Freire Veiga Sanjay Bezerra Bruna 《Primates; journal of primatology》2021,62(3):463-466
Primates - We describe predation on an adult rodent rock cavy and sharing of the carcass by a group of male bearded capuchins. Despite many studies, such an interaction has never been observed in... 相似文献
630.
Guevara Armando Vicente Vânia Aparecida de Souza Lima Bruna Jacomel F. Nery Andréia Ferreira Hagen Ferry Hahn Rosane Christine 《Mycopathologia》2022,187(4):363-374
Mycopathologia - Chromoblastomycosis and leprosy are chronic diseases with high prevalence in tropical and subtropical regions. Brazil is one of the countries with the highest incidence and... 相似文献