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排序方式: 共有195条查询结果,搜索用时 15 毫秒
151.
Sophie K. Jurgensen Simon Roux Sarah M. Schwenck Frank J. Stewart Matthew B. Sullivan Jennifer R. Brum 《The ISME journal》2022,16(4):972
Microbial communities in oxygen minimum zones (OMZs) are known to have significant impacts on global biogeochemical cycles, but viral influence on microbial processes in these regions are much less studied. Here we provide baseline ecological patterns using microscopy and viral metagenomics from the Eastern Tropical North Pacific (ETNP) OMZ region that enhance our understanding of viruses in these climate-critical systems. While extracellular viral abundance decreased below the oxycline, viral diversity and lytic infection frequency remained high within the OMZ, demonstrating that viral influences on microbial communities were still substantial without the detectable presence of oxygen. Viral community composition was strongly related to oxygen concentration, with viral populations in low-oxygen portions of the water column being distinct from their surface layer counterparts. However, this divergence was not accompanied by the expected differences in viral-encoded auxiliary metabolic genes (AMGs) relating to nitrogen and sulfur metabolisms that are known to be performed by microbial communities in these low-oxygen and anoxic regions. Instead, several abundant AMGs were identified in the oxycline and OMZ that may modulate host responses to low-oxygen stress. We hypothesize that this is due to selection for viral-encoded genes that influence host survivability rather than modulating host metabolic reactions within the ETNP OMZ. Together, this study shows that viruses are not only diverse throughout the water column in the ETNP, including the OMZ, but their infection of microorganisms has the potential to alter host physiological state within these biogeochemically important regions of the ocean.Subject terms: Microbial biooceanography, Microbial ecology 相似文献
152.
M Kale R Ramsey-Goldman S Bernatsky MB Urowitz D Gladman PR Fortin M Petri E Yelin S Manzi S Edworthy O Nived S-C Bae D Isenberg A Rahman JG Hanly C Gordon S Jacobsen E Ginzler DJ Wallace GS Alarcón MA Dooley L Gottesman K Steinsson A Zoma J-L Senécal S Barr G Sturfelt L Dreyer L Criswell J Sibley JL Lee AE Clarke 《Arthritis research & therapy》2012,14(Z3):A15
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154.
Amanda J.C. Brum Luiza Loebens Cynthia P. A. Prado Sonia Z. Cechin 《Acta zoologica》2022,103(1):99-111
Studies on the reproductive biology and age of amphibians provide primary information about the life history and population demographic parameters of species. Here, we describe the reproductive cycle, size–fecundity relationships, reproductive effort, sexual dimorphism and sexual maturity of Odontophrynus americanus, the flood frog, from South Brazil. A total of 96 individuals were analysed. The reproductive cycles of males and females were described through morphoanatomical analysis of testis and ovary. Age at onset of sexual maturity and estimated longevity were determined by skeletochronology. Individuals of O. americanus presented a potentially continuous reproductive cycle with a peak of reproductive activity in the warmer months. Females presented a higher reproductive investment than males. Sexual maturity was reached at around one year of age for both sexes while longevity differed between the sexes, with females living up to six years and males up to ten years. No evidence of sexual size dimorphism was found. This study is among the few that have assessed age at sexual maturity and longevity in a Neotropical anuran. Basic aspects of life history are of paramount importance because they allow comparisons and test of hypotheses to be made, which can help to build generalizations about the evolutionary meaning of ecological strategies. 相似文献
155.
Microbes drive myriad ecosystem processes, and their viruses modulate microbial-driven processes through mortality, horizontal gene transfer, and metabolic reprogramming by viral-encoded auxiliary metabolic genes (AMGs). However, our knowledge of viral roles in the oceans is primarily limited to surface waters. Here we assess the depth distribution of protein clusters (PCs) in the first large-scale quantitative viral metagenomic data set that spans much of the pelagic depth continuum (the Pacific Ocean Virome; POV). This established ‘core'' (180 PCs; one-third new to science) and ‘flexible'' (423K PCs) community gene sets, including niche-defining genes in the latter (385 and 170 PCs are exclusive and core to the photic and aphotic zones, respectively). Taxonomic annotation suggested that tailed phages are ubiquitous, but not abundant (<5% of PCs) and revealed depth-related taxonomic patterns. Functional annotation, coupled with extensive analyses to document non-viral DNA contamination, uncovered 32 new AMGs (9 core, 20 photic and 3 aphotic) that introduce ways in which viruses manipulate infected host metabolism, and parallel depth-stratified host adaptations (for example, photic zone genes for iron–sulphur cluster modulation for phage production, and aphotic zone genes for high-pressure deep-sea survival). Finally, significant vertical flux of photic zone viruses to the deep sea was detected, which is critical for interpreting depth-related patterns in nature. Beyond the ecological advances outlined here, this catalog of viral core, flexible and niche-defining genes provides a resource for future investigation into the organization, function and evolution of microbial molecular networks to mechanistically understand and model viral roles in the biosphere. 相似文献
156.
At the early onset of the 20th century, several studies already reported that the gray matter was implicated in the histopathology of multiple sclerosis
(MS). However, as white matter pathology long received predominant attention in this disease, and histological staining techniques
for detecting myelin in the gray matter were suboptimal, it was not until the beginning of the 21st century that the true extent and importance of gray matter pathology in MS was finally recognized. Gray matter damage was
shown to be frequent and extensive, and more pronounced in the progressive disease phases. Several studies subsequently demonstrated
that the histopathology of gray matter lesions differs from that of white matter lesions. Unfortunately, imaging of pathology
in gray matter structures proved to be difficult, especially when using conventional magnetic resonance imaging (MRI) techniques.
However, with the recent introduction of several more advanced MRI techniques, the detection of cortical and subcortical damage
in MS has considerably improved. This has important consequences for studying the clinical correlates of gray matter damage.
In this review, we provide an overview of what has been learned about imaging of gray matter damage in MS, and offer a brief
perspective with regards to future developments in this field. 相似文献
157.
Carlos R. Bueno Júnior Lucas C. Pantale?o Vanessa A. Voltarelli Luiz Henrique M. Bozi Patricia Chakur Brum Mayana Zatz 《PloS one》2012,7(9)
The present investigation was undertaken to test whether exercise training (ET) associated with AMPK/PPAR agonists (EM) would improve skeletal muscle function in mdx mice. These drugs have the potential to improve oxidative metabolism. This is of particular interest because oxidative muscle fibers are less affected in the course of the disease than glycolitic counterparts. Therefore, a cohort of 34 male congenic C57Bl/10J mdx mice included in this study was randomly assigned into four groups: vehicle solution (V), EM [AICAR (AMPK agonist, 50 mg/Kg-1.day-1, ip) and GW 1516 (PPARδ agonist, 2.5 mg/Kg-1.day-1, gavage)], ET (voluntary running on activity wheel) and EM+ET. Functional performance (grip meter and rotarod), aerobic capacity (running test), muscle histopathology, serum creatine kinase (CK), levels of ubiquitined proteins, oxidative metabolism protein expression (AMPK, PPAR, myoglobin and SCD) and intracellular calcium handling (DHPR, SERCA and NCX) protein expression were analyzed. Treatments started when the animals were two months old and were maintained for one month. A significant functional improvement (p<0.05) was observed in animals submitted to the combination of ET and EM. CK levels were decreased and the expression of proteins related to oxidative metabolism was increased in this group. There were no differences among the groups in the intracellular calcium handling protein expression. To our knowledge, this is the first study that tested the association of ET with EM in an experimental model of muscular dystrophy. Our results suggest that the association of ET and EM should be further tested as a potential therapeutic approach in muscular dystrophies. 相似文献
158.
159.
Juciano Gasparotto Mario Roberto Senger Emilio Telles de S Moreira Pedro Ozorio Brum Flvio Gabriel Carazza Kessler Daniel Oppermann Peixoto Alana Castro Panzenhagen Lin Kooi Ong Marlene Campos Soares Patricia Alves Reis Giuliana Viegas Schirato Walter Csar Ges Valente Bogar Omar Araújo Montoya Floriano P. Silva Jos Claudio Fonseca Moreira Felipe Dal-Pizzol Hugo C. Castro-Faria-Neto Daniel Pens Gelain 《The Journal of biological chemistry》2021,297(2)
160.