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961.
962.
Jose Serrano LeeAnn Higgins Bruce A. Witthuhn Lorraine B. Anderson Todd Markowski Gary W. Holcombe Patricia A. Kosian Joseph J. Korte Joseph E. Tietge Sigmund J. Degitz 《Comparative biochemistry and physiology. Part D, Genomics & proteomics》2010,5(2):138-150
As part of a multi-endpoint systems approach to develop comprehensive methods for assessing endocrine stressors in vertebrates, differential protein profiling was used to investigate expression patterns in the brain of the amphibian model (Xenopus laevis) following in vivo exposure to a suite of T4 synthesis inhibitors. We specifically address the application of Two Dimensional Polyacrylamide Gel Electrophoresis (2D PAGE), Isobaric Tags for Relative and Absolute Quantitation (iTRAQ®) and LC–MS/MS to assess changes in relative protein expression levels. 2D PAGE and iTRAQ proved to be effective complementary techniques for distinguishing protein changes in the developing amphibian brain in response to T4 synthesis inhibition. This information served to evaluate the use of distinctive protein profiles as a potential mechanism to screen chemicals for endocrine activity in anurans. Regulatory pathways associated with proteins expressed as a result of chemical effect are reported. To our knowledge, this is also the first account of the anuran larvae brain proteome characterization using proteomic technologies. Correlation of protein changes to other cellular and organism-level responses will aid in the development of a more rapid and cost-effective, non-mammalian screening assay for thyroid axis-disrupting chemicals. 相似文献
963.
Stone KL Bjornson RD Blasko GG Bruce C Cofrancesco R Carriero NJ Colangelo CM Crawford JK Crawford JM daSilva NC Deluca JD Elliott JI Elliott MM Flory PJ Folta-Stogniew EJ Gulcicek E Kong Y Lam TT Lee JY Lin A LoPresti MB Mane SM McMurray WJ Tikhonova IR Westman S Williams NA Wu TL Hongyu Z Williams KR 《The Yale journal of biology and medicine》2007,80(4):195-211
964.
Classification and prediction of clinical Alzheimer's diagnosis based on plasma signaling proteins 总被引:10,自引:0,他引:10
Ray S Britschgi M Herbert C Takeda-Uchimura Y Boxer A Blennow K Friedman LF Galasko DR Jutel M Karydas A Kaye JA Leszek J Miller BL Minthon L Quinn JF Rabinovici GD Robinson WH Sabbagh MN So YT Sparks DL Tabaton M Tinklenberg J Yesavage JA Tibshirani R Wyss-Coray T 《Nature medicine》2007,13(11):1359-1362
A molecular test for Alzheimer's disease could lead to better treatment and therapies. We found 18 signaling proteins in blood plasma that can be used to classify blinded samples from Alzheimer's and control subjects with close to 90% accuracy and to identify patients who had mild cognitive impairment that progressed to Alzheimer's disease 2-6 years later. Biological analysis of the 18 proteins points to systemic dysregulation of hematopoiesis, immune responses, apoptosis and neuronal support in presymptomatic Alzheimer's disease. 相似文献
965.
966.
Christine P. Diggle Daniel J. Moore Girish Mali Petra zur Lage Aouatef Ait-Lounis Miriam Schmidts Amelia Shoemark Amaya Garcia Munoz Mihail R. Halachev Philippe Gautier Patricia L. Yeyati David T. Bonthron Ian M. Carr Bruce Hayward Alexander F. Markham Jilly E. Hope Alex von Kriegsheim Hannah M. Mitchison Ian J. Jackson Bénédicte Durand Walter Reith Eamonn Sheridan Andrew P. Jarman Pleasantine Mill 《PLoS genetics》2014,10(9)
967.
Johansson LC Arnlund D White TA Katona G Deponte DP Weierstall U Doak RB Shoeman RL Lomb L Malmerberg E Davidsson J Nass K Liang M Andreasson J Aquila A Bajt S Barthelmess M Barty A Bogan MJ Bostedt C Bozek JD Caleman C Coffee R Coppola N Ekeberg T Epp SW Erk B Fleckenstein H Foucar L Graafsma H Gumprecht L Hajdu J Hampton CY Hartmann R Hartmann A Hauser G Hirsemann H Holl P Hunter MS Kassemeyer S Kimmel N Kirian RA Maia FR Marchesini S Martin AV Reich C Rolles D Rudek B Rudenko A Schlichting I 《Nature methods》2012,9(3):263-265
X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet. 相似文献
968.
969.
David A. Mead Susan Lucas Alex Copeland Alla Lapidus Jan-Feng Cheng David C. Bruce Lynne A. Goodwin Sam Pitluck Olga Chertkov Xiaojing Zhang John C. Detter Cliff S. Han Roxanne Tapia Miriam Land Loren J. Hauser Yun-juan Chang Nikos C. Kyrpides Natalia N. Ivanova Galina Ovchinnikova Tanja Woyke Catherine Brumm Rebecca Hochstein Thomas Schoenfeld Phillip Brumm 《Standards in genomic sciences》2012,6(3):381-400
970.
Thomas F. Hourigan Frank G. Stanton Philip J. Motta Christopher D. Kelley Bruce Carlson 《Environmental Biology of Fishes》1989,24(2):105-116
Synopsis The foraging behavior and associated morphology of the feeding apparatus of three sympatric species of angelfishes, Holacanthus tricolor, Pomacanthus arcuatus and Pomacanthus paru were studied at St. Croix, U.S. Virgin Islands. All three had overlapping diets, consisting of algae and numerous species of sponges. The two Pomacanthus species also fed on gorgonians. The morphology of the dentition, jaws and gill rakers was similar in all three species. Male Holacanthus tricolor defended territories overlapping the foraging areas of two to four females. Within the male's territory, females defended smaller territories against other females of the same size, but tolerated smaller females. In contrast, both Pomacanthus spp. formed pairs which defended intraspecific feeding territories. 相似文献