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181.
Polyuronic acids produced by Pseudomonas aeruginosa 总被引:14,自引:0,他引:14
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CHROMOSOME VELOCITY DURING MITOSIS AS A FUNCTION OF CHROMOSOME SIZE AND POSITION 总被引:2,自引:1,他引:1 下载免费PDF全文
R. Bruce Nicklas 《The Journal of cell biology》1965,25(1):119-135
Chromosome velocity has been studied in living Melanoplus differentialis spermatocytes by phase contrast cinemicrography. Melanoplus chromosomes (and bivalents) differ in length by as much as 1:3.5. As expected, no size-dependent velocity differences were detected in anaphase, and this is also shown to be true for the less predictable movements during prometaphase congression. The size of the X chromosome can change during observation following x-irradiation, but this is equally without influence on velocity. However, an effect of position on velocity is found in both prometaphase and in anaphase: the chromosomes furthest from the central interpolar axis move 25 per cent faster than more central chromosomes. A simple mechanical model relating frictional resistance and mitotic forces to chromosome velocity is discussed in detail. Calculations from the model suggest that a significant difference in the force acting on a large, as compared with a small chromosome is necessary to account for the observed similarity in velocity. Therefore, it is concluded that the mitotic forces are so organized or regulated that velocity is, within limits, independent of load. The implications of velocity-load independence in relation to the molecular origin of mitotic forces are discussed. 相似文献
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The iodoacetate-nitrogen-poisoned muscle offers the possibility of studying the stoichiometry of the single muscle twitch since metabolic resynthesis by glycolysis and oxidative phosphorylation are blocked, and there remains as an energy source only the creatine phosphoryltransfer system, creatine phosphate reacting with adenosinediphosphate to give the triphosphate and creatine. It is shown, preparatory to a determination of the amount of phosphocreatine split in a single twitch, that iodoacetate does not inhibit creatine phosphoryltransferase at concentrations which block glycolysis. An analysis is developed which assumes that the transferase maintains the creatine phosphoryl transfer reaction in equilibrium following contraction, and further that the creatine phosporyltransfer reaction and the myokinase reaction are isolated in muscle. On the basis of this analysis and the data obtained, an estimate of the equilibrium constant of the creatine phosphoryl reaction in muscle is obtained which agrees with values determined in vitro. Using the estimated equilibrium constant, and the concentrations of creatine, creatine phosphate, and adenosinetriphosphate found, a value for the concentration of free adenosinediphosphate is obtained which is considerably less than that found by direct chemical analysis. 相似文献
188.
Aspects of the life cycle of marine nematodes 总被引:1,自引:0,他引:1
Summary 1. Life cycles of approximately one month are recorded for laboratory maintained cultures of chromadorid, monhysterid and oncholaimid nematodes.2. Amphimictic and parthenogenetic reproduction occurs in the species investigated. Reproduction inMonhystera parelegantula is by parthenogenesis.3. The chromadoridChromadorina epidemos and the oncholaimidViscosia macramphida are able to reproduce by either amphimixis or parthenogenesis.4.Acanthonchus cobbi, Chromadora macrolaimoides andEuchromadora gaulica are amphimitically reproducing species.
Aspekte des Lebenszyklus mariner Nematoden
Kurzfassung Bei einigen marinen Nematoden, die in Biscayne Bay (Florida) gesammelt und im Labor gezüchtet worden sind, vollzieht sich der gesamte Lebenszyklus ungefähr innerhalb eines Monats. BeiAcanthonchus cobbi, Chromadora macrolaimoides undEuchromadora gaulica ist zweigeschlechtliche Fortpflanzung festgestellt worden.Monohystera parelegantula vermehrt sich parthenogenetisch. Ein möglicherweise durch Umwelteinflüsse bedingter Wechsel von bisexueller und parthenogenetischer Fortpflanzung tritt beiChromadora epidemos undViscosia macramphida auf.相似文献
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Summary 1. Studies of the broad-leafed turtle grass,Thalassia testudinum
König, have revealed a diverse range of fungal infestation different in generic composition and dynamics of attack from that found on submerged wood. Certain of the fungi, notably the AscomyceteLindra thalassiae, initiate considerable degradation of leaf tissue and show a developmental cycle in nature related to the physiological state of the host plant.2. Use of fungal-cellulose mats as a trapping substrate has been extremely effective for discernment of ecologically significant shifts in nematode concentrations, especially those of the omnivorous species,Metoncholaimus scissus.3. Patterns of activity ofM. scissus, as well as those of various foliicolous nematodes, suggest that loci of organic material, such as fungal infested leaves and decaying plant tissue, significantly affect biological activity of these animals.4. Laboratory analysis of degraded cotton cellulose filters show a striking incidence of fungal reproduction of the ascomycetous fungusLulworthia, along with development of a considerable associated nematode fauna, especially species ofViscosia (V. macramphida) andLeptolaimus (L. plectoides). Successional patterns in nematode development are noted with continued degradation of the cotton cellulose matrix.
Contribution No. 768 from the Institute of Marine Science, University of Miami, Miami, Florida, and from the Canada Department of Agriculture, Ottawa, Canada. This work was supported at the IMS by grant GM 12842 from the National Institutes of Health, USA. 相似文献
Studien über marine Pilz-Nematoden-Assoziationen und Pflanzendegradation
Kurzfassung Untersuchungen am SeegrasThalassia testudinum König haben ergeben, daß sich hier Pilzinfektionen hinsichtlich der Komposition der beteiligten Gattungen und der Dynamik des Befalls von den am untergetauchten Holz festgestellten Infektionen unterscheiden. Bestimmte Pilze, insbesondere der AscomycetLindra thalassiae, leiten eine erhebliche Degradation des Blattgewebes ein und zeigen einen Entwicklungszyklus, welcher in Beziehung steht zum physiologischen Zustand der Wirtspflanze. Die Anwendung von Pilz-Zellulose-Matten als Einfangsubstrat war außerordentlich erfolgreich für das Erkennen ökologisch signifikanter Verschiebungen in den Nematodenkonzentrationen, insbesondere bei der omnivoren ArtMetoncholaimus scissus. Die Aktivitätsmuster vonM. scissus — ebenso wie die verschiedener foliicolöser Nematoden — deuten darauf hin, daß pilzinfizierte und zerfallende Pflanzenteile in entscheidendem Maße die biologische Aktivität dieser Tiere beeinflussen. Laboratoriumsanalysen degradierter Wollzellulosefilter lassen eine überraschend starke Vermehrung des AscomycetenLulworthia erkennen und gleichzeitig die Entwicklung einer beachtlichen Fauna assoziierter Nematodenarten, insbesondere vonViscosia macramphida undLeptolaimus plectoides. Im Verlaufe der weiteren Degradation der Wollzellulosematrix kommt es bei der Nematodenfauna zu entsprechenden Sukzessionen.
Contribution No. 768 from the Institute of Marine Science, University of Miami, Miami, Florida, and from the Canada Department of Agriculture, Ottawa, Canada. This work was supported at the IMS by grant GM 12842 from the National Institutes of Health, USA. 相似文献
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