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51.
Ruthenium red stainable surface layer on lung alveolar cells; electron microscopic interpretation 总被引:5,自引:0,他引:5
R E Brooks 《Stain technology》1969,44(4):173-177
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Robert E. Brooks 《Cell and tissue research》1970,106(4):484-497
Summary An ultrastructural comparison of mammalian, reptilian, and amphibian lung alveolar cells, and avian lung atrial cells reveals that morphologically similar cytoplasmic bodies (cytosomes) occur in these cells. The cytosomes, which appear generally as osmiophilic, lamellae-containing, membrane-bound, round bodies 0.3 to 0.5 in diameter, are also similar to bodies occurring in epithelial cells of both physoclistous and physostomatous swimbladders of fishes. Because the function of both lung alveolar and swimbladder epithelial cells is gas-handling, the possibility is raised that the morphologically similar lamellae-containing bodies of these vertebrate cells are functionally identical. One function, suggested by other investigators, is that, in mammalian lungs, these bodies supply a surface-tension lowering material (surfactant). Because several assumptions concerning this proposed function remain unproved, an alternative proposal is speculatively explored. The suggestion is offered that cytosomes contain an antioxidant needed to protect alveolar and swimbladder cells against the toxic effects of the relatively high concentration of oxygen to which these cells are exposed.Supported by a research grant from the American Cancer Society, Oregon Division, Inc. 相似文献
53.
Robert E. Brooks 《Cell and tissue research》1970,106(4):473-483
Summary Rainbow trout swimbladder epithelium consists of non-ciliated and ciliated cells in the ratio of greater than 21. Non-ciliated cells contain vesicles filled with a mucus-like material and similar material is found lining the surface of the swimbladder lumen. Morphological evidence for discharge of the vesicle contents was obtained. In addition, nonciliated cells contain osmiophilic lamellar bodies which resemble the cytosomes of lung alveolar cells of air-breathing vertebrates. The non-ciliated cells do not appear to be involved in a process of active gas secretion.Supported by a research grant from the American Cancer Society, Oregon Division, Inc. 相似文献
54.
Inversion of Transfer Modes and Sex Factor-Chromosome Interactions in Conjugation in Escherichia coli 总被引:8,自引:4,他引:4
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Brooks Low 《Journal of bacteriology》1967,93(1):98-106
A study was made of the mating properties of an unusual system of interconvertible donor strains of Escherichia coli K-12: Ra-1, Ra-2, and RaF+. The Ra-1 and Ra-2 strains are Hfr strains whose origins are widely separated on the chromosome and whose transfer modes proceed in the opposite direction from one another. When Ra-1 cells were mated with females, a small fraction of the donors transferred markers via the Ra-2 mode. This effect was enhanced by preconjugal ultraviolet (UV) treatment of the Ra-1 cells. Among the survivors of UV-treated Ra-1 cells, a few stable Ra-2 cells were found. When Ra-2 cells were used as the donors, some of them were found to mate via the Ra-1 mode, in analogy with the Ra-1 to Ra-2 alteration with inversion of F mentioned above. Related experiments suggested that the inversion occurs by detachment of the F factor from one Hfr origin locus, followed by reassociation of the F factor with the other Hfr origin locus. Both the Ra-1 and Ra-2 strains reverted spontaneously to an F+ strain, called RaF+. Cultures of RaF+ cells were found to mate primarily according to the Ra-1 and Ra-2 transfer modes, with smaller contributions also coming from transfer modes with origins elsewhere on the chromosome in a way which is similar to the transfer of markers from a normal F+ strain. The RaF+ sex factor was found to be wild type, whereas the chromosome was found to carry irregularities (sex factor affinity loci) at the locations of the Ra-1 and Ra-2 origins. Only about 10% of the donor capacity of the RaF+ strain was due to stable spontaneous Hfr cells in cultures of RaF+ cells. 相似文献
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The effects of truncating long-range forces on protein dynamics 总被引:8,自引:0,他引:8
This paper considers the effects of truncating long-range forces on protein dynamics. Six methods of truncation that we investigate as a function of cutoff criterion of the long-range potentials are (1) a shifted potential; (2) a switching function; (3) simple atom-atom truncation based on distance; (4) simple atom-atom truncation based on a list which is updated periodically (every 25 steps); (5) simple group-group truncation based on distance; and (6) simple group-group truncation based on a list which is updated periodically (every 25 steps). Based on 70 calculations of carboxymyoglobin we show that the method and distance of long range cutoff have a dramatic effect on overall protein behavior. Evaluation of the different methods is based on comparison of a simulation's rms fluctuation about the average coordinates, the rms deviation from the average coordinates of a no cutoff simulation and from the X-ray structure of the protein. The simulations in which long-range forces are truncated by a shifted potential shows large rms deviations for cutoff criteria less than 14 A, and reasonable deviations and fluctuations at this cutoff distance or larger. Simulations using a switching function are investigated by varying the range over which electrostatic interactions are switched off. Results using a short switching function that switches off the potential over a short range of distances are poor for all cutoff distances. A switching function over a 5-9 A range gives reasonable results for a distance-dependent dielectric, but not using a constant dielectric. Both the atom-atom and group-group truncation methods based on distance shows large rms deviation and fluctuation for short cutoff distances, while for cutoff distances of 11 A or greater, reasonable results are achieved. Although comparison of these to distance-based truncation methods show surprisingly larger rms deviations for the group-group truncation, contrary to simulation studies of aqueous ionic solutions. The results of atom-atom or group-group list-based simulations generally appear to be less stable than the distance-based simulations, and require more frequent velocity scaling or stronger coupling to a heat bath. 相似文献