全文获取类型
收费全文 | 1747篇 |
免费 | 200篇 |
出版年
2022年 | 20篇 |
2021年 | 31篇 |
2020年 | 16篇 |
2019年 | 26篇 |
2018年 | 33篇 |
2017年 | 23篇 |
2016年 | 34篇 |
2015年 | 61篇 |
2014年 | 63篇 |
2013年 | 82篇 |
2012年 | 79篇 |
2011年 | 100篇 |
2010年 | 59篇 |
2009年 | 48篇 |
2008年 | 73篇 |
2007年 | 83篇 |
2006年 | 57篇 |
2005年 | 60篇 |
2004年 | 63篇 |
2003年 | 64篇 |
2002年 | 69篇 |
2001年 | 48篇 |
2000年 | 50篇 |
1999年 | 53篇 |
1998年 | 20篇 |
1997年 | 19篇 |
1996年 | 19篇 |
1994年 | 13篇 |
1993年 | 15篇 |
1992年 | 31篇 |
1991年 | 25篇 |
1990年 | 30篇 |
1989年 | 39篇 |
1988年 | 30篇 |
1987年 | 23篇 |
1986年 | 21篇 |
1985年 | 19篇 |
1984年 | 20篇 |
1982年 | 19篇 |
1981年 | 25篇 |
1980年 | 14篇 |
1979年 | 16篇 |
1978年 | 12篇 |
1975年 | 14篇 |
1974年 | 17篇 |
1973年 | 21篇 |
1972年 | 15篇 |
1970年 | 14篇 |
1967年 | 16篇 |
1966年 | 16篇 |
排序方式: 共有1947条查询结果,搜索用时 31 毫秒
71.
Cristin J. Monaco Corey J. A. Bradshaw David J. Booth Bronwyn M. Gillanders David S. Schoeman Ivan Nagelkerken 《Global Change Biology》2020,26(10):5564-5573
Climate change is redistributing marine and terrestrial species globally. Life‐history traits mediate the ability of species to cope with novel environmental conditions, and can be used to gauge the potential redistribution of taxa facing the challenges of a changing climate. However, it is unclear whether the same traits are important across different stages of range shifts (arrival, population increase, persistence). To test which life‐history traits most mediate the process of range extension, we used a 16‐year dataset of 35 range‐extending coral‐reef fish species and quantified the importance of various traits on the arrival time (earliness) and degree of persistence (prevalence and patchiness) at higher latitudes. We show that traits predisposing species to shift their range more rapidly (large body size, broad latitudinal range, long dispersal duration) did not drive the early stages of redistribution. Instead, we found that as diet breadth increased, the initial arrival and establishment (prevalence and patchiness) of climate migrant species in temperate locations occurred earlier. While the initial incursion of range‐shifting species depends on traits associated with dispersal potential, subsequent establishment hinges more on a species’ ability to exploit novel food resources locally. These results highlight that generalist species that can best adapt to novel food sources might be most successful in a future ocean. 相似文献
72.
Jinger S. Gottschall Riley C. Sheehan Danielle S. Downs 《Journal of electromyography and kinesiology》2013,23(5):1237-1242
Falls are the leading cause of nonfatal injury across all age groups and a common incident for pregnant women. Thus, there is a critical demand for research to evaluate if walking strategies in pregnant women change throughout pregnancy in order to effectively intervene and minimize the incidence rate. The aim of the present study was to analyze modifications in temporal–spatial parameters as well as muscle activity during hill walking transitions in pregnant women between gestational week 20 and 32. Based upon previous literature, we hypothesized that in comparison to level walking, the transition strides of pregnant women would be distinct between trimesters in order to accommodate the physical changes within twelve weeks. Thirteen pregnant women completed a series of randomly assigned walking conditions on level and hill surfaces during gestational week 20 and 32. Our results demonstrated that pregnant women modulated their gait patterns throughout pregnancy with additional joint flexion as well as muscle activity at the ankle, knee and hip. In summary, pregnant women exaggerate cautious gait patterns by walking slower and wider with greater joint flexion and muscle activity in order to safely transition between level and hill surfaces. 相似文献
73.
Riley C. Sheehan Jinger S. Gottschall 《Journal of electromyography and kinesiology》2013,23(4):826-830
It is well established that metabolic cost is minimized at an individual’s running preferred step frequency (PSF). It has been proposed that the metabolic minimum at PSF is due to a tradeoff between mechanical factors, however, this ignores muscle activity, the primary consumer of energy. Thus, we hypothesized that during downhill running, total muscle activity would be greater with deviations from PSF. Specifically, we predicted that slow step frequencies would have greater stance activity while fast step frequencies would have greater swing activity. We collected metabolic cost and leg muscle activity data while 10 healthy young adults ran at 3.0 m/s for 5 min at level and downhill at PSF and ±15% PSF. In support of our hypothesis, there was a significant main effect for step frequency for both metabolic cost and total muscle activity. In addition, there was greater muscle activity in the stance phase during the slower step frequency while muscle activity was greater in the swing phase during the fast step frequency. This suggests that PSF is partially determined by the tradeoff between the greater cost of muscle activity in the swing phase and lower cost in the stance phase with faster step frequency. 相似文献
74.
Sally A. Cantrell Michael D. Leavell Olivera Marjanovic Anthony T. Iavarone Julie A. Leary Lee W. Riley 《Journal of microbiology (Seoul, Korea)》2013,51(5):619-626
The lipid-rich cell wall of Mycobacterium tuberculosis, the agent of tuberculosis, serves as an effective barrier against many chemotherapeutic agents and toxic host cell effector molecules, and it may contribute to the mechanism of persistence. Mycobacterium tuberculosis strains mutated in a 13-gene operon called mce1, which encodes a putative ABC lipid transporter, induce aberrant granulomatous response in mouse lungs. Because of the postulated role of the mce1 operon in lipid importation, we compared the cell wall lipid composition of wild type and mce1 operon mutant M. tuberculosis H37Rv strains. High resolution mass spectrometric analyses of the mce1 mutant lipid extracts showed unbound mycolic acids to accumulate in the cell wall. Quantitative analysis revealed a 10.7 fold greater amount of free mycolates in the mutant compared to that of the wild type strain. The free mycolates were comprised of alpha, methoxy and keto mycolates in the ratio 1:0.9:0.6, respectively. Since the mce1 operon is regulated in vivo, the free mycolates that accumulate during infection may serve as a barrier for M. tuberculosis against toxic products and contribute to the pathogen’s persistence. 相似文献
75.
Ana Villegas-Mendez Emily Gwyer Findlay J. Brian de Souza Lisa-Marie Grady Christiaan J. Saris Thomas E. Lane Eleanor M. Riley Kevin N. Couper 《PloS one》2013,8(11)
IL-27 is an important and non-redundant regulator of effector T cell accumulation in non-lymphoid tissues during infection. Using malaria as a model systemic pro-inflammatory infection, we demonstrate that the aberrant accumulation of CD4+ T cells in the liver of infected IL27R−/− (WSX-1−/−) mice is a result of differences in cellular recruitment, rather than changes in T cell proliferation or cell death. We show that IL-27 both inhibits the migratory capacity of infection-derived CD4+ T cells towards infection-derived liver cells, but also suppresses the production of soluble liver-derived mediator(s) that direct CD4+ T cell movement towards the inflamed tissue. Although CCL4 and CCL5 expression was higher in livers of infected WSX-1−/− mice than infected WT mice, and hepatic CD4+ T cells from WSX-1−/− mice expressed higher levels of CCR5 than cells from WT mice, migration of CD4+ T cells to the liver of WSX-1−/− mice during infection was not controlled by chemokine (R) signalling. However, anti-IL-12p40 treatment reduced migration of CD4+ T cells towards infection-derived liver cells, primarily by abrogating the hepatotropic migratory capacity of T cells, rather than diminishing soluble tissue-derived migratory signals. These results indicate that IL-27R signalling restricts CD4+ T cell accumulation within the liver during infection primarily by suppressing T cell chemotaxis, which may be linked to its capacity to repress Th1 differentiation, as well as by inhibiting the production of soluble, tissue-derived chemotaxins. 相似文献
76.
Kevin E. Riley Jane S. Murray Jindřich Fanfrlík Jan Řezáč Ricardo J. Solá Monica C. Concha Felix M. Ramos Peter Politzer 《Journal of molecular modeling》2013,19(11):4651-4659
In a previous study we investigated the effects of aromatic fluorine substitution on the strengths of the halogen bonds in halobenzene…acetone complexes (halo?=?chloro, bromo, and iodo). In this work, we have examined the origins of these halogen bonds (excluding the iodo systems), more specifically, the relative contributions of electrostatic and dispersion forces in these interactions and how these contributions change when halogen σ-holes are modified. These studies have been carried out using density functional symmetry adapted perturbation theory (DFT-SAPT) and through analyses of intermolecular correlation energies and molecular electrostatic potentials. It is found that electrostatic and dispersion contributions to attraction in halogen bonds vary from complex to complex, but are generally quite similar in magnitude. Not surprisingly, increasing the size and positive nature of a halogen’s σ-hole dramatically enhances the strength of the electrostatic component of the halogen bonding interaction. Not so obviously, halogens with larger, more positive σ-holes tend to exhibit weaker dispersion interactions, which is attributable to the lower local polarizabilities of the larger σ-holes. Figure
In this work we investigate the roles played by electrostatic and dispersion forces in stabilizing halogen bonding interactions. 相似文献
77.
Ganesh Kumar Agrawal Dominique Job Thomas Kieselbach Bronwyn J. Barkla Sixue Chen Renu Deswal Sabine Lüthje Ramesh Sundar Amalraj Georgia Tanou Bongani Kaiser Ndimba Rainer Cramer Wolfram Weckwerth Stefanie Wienkoop Michael J. Dunn Sun Tae Kim Yochiro Fukao Masami Yonekura Lello Zolla Jai Singh Rohila Rungaroon Waditee‐Sirisattha Antonio Masi Tai Wang Abhijit Sarkar Raj Agrawal Jenny Renaut Randeep Rakwal 《Proteomics》2013,13(21):3093-3100
The International Plant Proteomics Organization (INPPO) is a non‐profit organization whose members are scientists involved or interested in plant proteomics. Since the publication of the first INPPO highlights in 2012, continued progress on many of the organization's mandates/goals has been achieved. Two major events are emphasized in this second INPPO highlights. First, the change of guard at the top, passing of the baton from Dominique Job, INPPO founding President to Ganesh Kumar Agrawal as the incoming President. Ganesh K. Agrawal, along with Dominique Job and Randeep Rakwal initiated the INPPO. Second, the most recent INPPO achievements and future targets, mainly the organization of first the INPPO World Congress in 2014, tentatively planned for Hamburg (Germany), are mentioned. 相似文献
78.
79.
Paul J. Norman Jill A. Hollenbach Neda Nemat-Gorgani Lisbeth A. Guethlein Hugo G. Hilton Marcelo J. Pando Kwadwo A. Koram Eleanor M. Riley Laurent Abi-Rached Peter Parham 《PLoS genetics》2013,9(10)
Interactions between HLA class I molecules and killer-cell immunoglobulin-like receptors (KIR) control natural killer cell (NK) functions in immunity and reproduction. Encoded by genes on different chromosomes, these polymorphic ligands and receptors correlate highly with disease resistance and susceptibility. Although studied at low-resolution in many populations, high-resolution analysis of combinatorial diversity of HLA class I and KIR is limited to Asian and Amerindian populations with low genetic diversity. At the other end of the spectrum is the West African population investigated here: we studied 235 individuals, including 104 mother-child pairs, from the Ga-Adangbe of Ghana. This population has a rich diversity of 175 KIR variants forming 208 KIR haplotypes, and 81 HLA-A, -B and -C variants forming 190 HLA class I haplotypes. Each individual we studied has a unique compound genotype of HLA class I and KIR, forming 1–14 functional ligand-receptor interactions. Maintaining this exceptionally high polymorphism is balancing selection. The centromeric region of the KIR locus, encoding HLA-C receptors, is highly diverse whereas the telomeric region encoding Bw4-specific KIR3DL1, lacks diversity in Africans. Present in the Ga-Adangbe are high frequencies of Bw4-bearing HLA-B*53:01 and Bw4-lacking HLA-B*35:01, which otherwise are identical. Balancing selection at key residues maintains numerous HLA-B allotypes having and lacking Bw4, and also those of stronger and weaker interaction with LILRB1, a KIR-related receptor. Correspondingly, there is a balance at key residues of KIR3DL1 that modulate its level of cell-surface expression. Thus, capacity to interact with NK cells synergizes with peptide binding diversity to drive HLA-B allele frequency distribution. These features of KIR and HLA are consistent with ongoing co-evolution and selection imposed by a pathogen endemic to West Africa. Because of the prevalence of malaria in the Ga-Adangbe and previous associations of cerebral malaria with HLA-B*53:01 and KIR, Plasmodium falciparum is a candidate pathogen. 相似文献
80.
While a suite of approaches have been developed to describe the scale, rate and spatial structure of exchange among populations, a lack of mechanistic understanding will invariably compromise predictions of population-level responses to ecosystem modification. In this study, we measured the energetics and sustained swimming capacity of giant Australian cuttlefish Sepia apama and combined these data with information on the life-history strategy, behaviour and circulation patterns experienced by the species to predict scales of connectivity throughout parts of their range. The swimming capacity of adult and juvenile S. apama was poor compared to most other cephalopods, with most individuals incapable of maintaining swimming above 15 cm s−1. Our estimate of optimal swimming speed (6–7 cm s−1) and dispersal potential were consistent with the observed fine-scale population structure of the species. By comparing observed and predicted population connectivity, we identified several mechanisms that are likely to have driven fine-scale population structure in this species, which will assist in the interpretation of future population declines. 相似文献