全文获取类型
收费全文 | 817篇 |
免费 | 99篇 |
专业分类
916篇 |
出版年
2023年 | 5篇 |
2021年 | 11篇 |
2020年 | 10篇 |
2019年 | 11篇 |
2018年 | 16篇 |
2017年 | 11篇 |
2016年 | 21篇 |
2015年 | 32篇 |
2014年 | 28篇 |
2013年 | 39篇 |
2012年 | 46篇 |
2011年 | 52篇 |
2010年 | 35篇 |
2009年 | 37篇 |
2008年 | 43篇 |
2007年 | 47篇 |
2006年 | 33篇 |
2005年 | 27篇 |
2004年 | 37篇 |
2003年 | 21篇 |
2002年 | 23篇 |
2001年 | 15篇 |
2000年 | 16篇 |
1999年 | 14篇 |
1998年 | 13篇 |
1997年 | 9篇 |
1996年 | 7篇 |
1994年 | 7篇 |
1992年 | 7篇 |
1991年 | 10篇 |
1990年 | 6篇 |
1989年 | 12篇 |
1986年 | 7篇 |
1985年 | 10篇 |
1984年 | 12篇 |
1983年 | 10篇 |
1980年 | 10篇 |
1978年 | 7篇 |
1976年 | 9篇 |
1975年 | 9篇 |
1973年 | 4篇 |
1972年 | 6篇 |
1971年 | 4篇 |
1970年 | 5篇 |
1968年 | 4篇 |
1967年 | 5篇 |
1966年 | 5篇 |
1959年 | 7篇 |
1939年 | 4篇 |
1908年 | 4篇 |
排序方式: 共有916条查询结果,搜索用时 15 毫秒
81.
82.
83.
84.
85.
86.
87.
88.
A variant of KCC2 from patients with febrile seizures impairs neuronal Cl− extrusion and dendritic spine formation 下载免费PDF全文
Martin Puskarjov Patricia Seja Sarah E Heron Tristiana C Williams Faraz Ahmad Xenia Iona Karen L Oliver Bronwyn E Grinton Laszlo Vutskits Ingrid E Scheffer Steven Petrou Peter Blaesse Leanne M Dibbens Samuel F Berkovic Kai Kaila 《EMBO reports》2014,15(6):723-729
Genetic variation in SLC12A5 which encodes KCC2, the neuron-specific cation-chloride cotransporter that is essential for hyperpolarizing GABAergic signaling and formation of cortical dendritic spines, has not been reported in human disease. Screening of SLC12A5 revealed a co-segregating variant (KCC2-R952H) in an Australian family with febrile seizures. We show that KCC2-R952H reduces neuronal Cl− extrusion and has a compromised ability to induce dendritic spines in vivo and in vitro. Biochemical analyses indicate a reduced surface expression of KCC2-R952H which likely contributes to the functional deficits. Our data suggest that KCC2-R952H is a bona fide susceptibility variant for febrile seizures. 相似文献
89.
Ye JM Iglesias MA Watson DG Ellis B Wood L Jensen PB Sørensen RV Larsen PJ Cooney GJ Wassermann K Kraegen EW 《American journal of physiology. Endocrinology and metabolism》2003,284(3):E531-E540
Peroxisome proliferator-activated receptor (PPAR)alpha and PPARgamma agonists lower lipid accumulation in muscle and liver by different mechanisms. We investigated whether benefits could be achieved on insulin sensitivity and lipid metabolism by the dual PPARalpha/gamma agonist ragaglitazar in high fat-fed rats. Ragaglitazar completely eliminated high-fat feeding-induced liver triglyceride accumulation and visceral adiposity, like the PPARalpha agonist Wy-14643 but without causing hepatomegaly. In contrast, the PPARgamma agonist rosiglitazone only slightly lessened liver triglyceride without affecting visceral adiposity. Compared with rosiglitazone or Wy-14643, ragaglitazar showed a much greater effect (79%, P < 0.05) to enhance insulin's suppression of hepatic glucose output. Whereas all three PPAR agonists lowered plasma triglyceride levels and lessened muscle long-chain acyl-CoAs, ragaglitazar and rosiglitazone had greater insulin-sensitizing action in muscle than Wy-14643, associated with a threefold increase in plasma adiponectin levels. There was a significant correlation of lipid content and insulin action in liver and particularly muscle with adiponectin levels (P < 0.01). We conclude that the PPARalpha/gamma agonist ragaglitazar has a therapeutic potential for insulin-resistant states as a PPARgamma ligand, with possible involvement of adiponectin. Additionally, it can counteract fatty liver, hepatic insulin resistance, and visceral adiposity generally associated with PPARalpha activation, but without hepatomegaly. 相似文献
90.
Liquid Serial Dilution Is Inferior to Solid Media for Isolation of Cultures Representative of the Phylum-Level Diversity of Soil Bacteria 总被引:2,自引:2,他引:2 下载免费PDF全文
Liesbeth Schoenborn Penelope S. Yates Bronwyn E. Grinton Philip Hugenholtz Peter H. Janssen 《Applied microbiology》2004,70(7):4363-4366
Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified versions of the same media. We conclude that, contrary to expectation, liquid serial dilution culture is inferior to culturing on solid media for isolating representatives of many bacterial phyla from soil. 相似文献