全文获取类型
收费全文 | 447篇 |
免费 | 58篇 |
专业分类
505篇 |
出版年
2022年 | 3篇 |
2021年 | 4篇 |
2019年 | 2篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 3篇 |
2015年 | 16篇 |
2014年 | 26篇 |
2013年 | 32篇 |
2012年 | 31篇 |
2011年 | 22篇 |
2010年 | 18篇 |
2009年 | 17篇 |
2008年 | 19篇 |
2007年 | 16篇 |
2006年 | 18篇 |
2005年 | 16篇 |
2004年 | 20篇 |
2003年 | 16篇 |
2002年 | 14篇 |
2001年 | 17篇 |
2000年 | 15篇 |
1999年 | 16篇 |
1998年 | 13篇 |
1997年 | 8篇 |
1996年 | 8篇 |
1995年 | 8篇 |
1994年 | 3篇 |
1993年 | 6篇 |
1992年 | 9篇 |
1991年 | 10篇 |
1990年 | 10篇 |
1989年 | 4篇 |
1988年 | 13篇 |
1987年 | 10篇 |
1986年 | 2篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 6篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1972年 | 4篇 |
1971年 | 2篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有505条查询结果,搜索用时 9 毫秒
41.
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome 下载免费PDF全文
Harold Tjalsma Haike Antelmann Jan D.H. Jongbloed Peter G. Braun Elise Darmon Ronald Dorenbos Jean-Yves F. Dubois Helga Westers Geeske Zanen Wim J. Quax Oscar P. Kuipers Sierd Bron Michael Hecker Jan Maarten van Dijl 《Microbiological reviews》2004,68(2):207-233
Secretory proteins perform a variety of important “remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make predictions about the composition of bacterial machinery for protein secretion as well as the extracellular complement of bacterial proteomes. Recently, the power of proteomics was successfully employed to evaluate genome-based models of these so-called secretomes. Progress in this field is well illustrated by the proteomic analysis of protein secretion by the gram-positive bacterium Bacillus subtilis, for which ~90 extracellular proteins were identified. Analysis of these proteins disclosed various “secrets of the secretome,” such as the residence of cytoplasmic and predicted cell envelope proteins in the extracellular proteome. This showed that genome-based predictions reflect only ~50% of the actual composition of the extracellular proteome of B. subtilis. Importantly, proteomics allowed the first verification of the impact of individual secretion machinery components on the total flow of proteins from the cytoplasm to the extracellular environment. In conclusion, proteomics has yielded a variety of novel leads for the analysis of protein traffic in B. subtilis and other gram-positive bacteria. Ultimately, such leads will serve to increase our understanding of virulence factor biogenesis in gram-positive pathogens, which is likely to be of high medical relevance. 相似文献
42.
Stephanie J. Salyer Esther M. Ellis Corvil Salomon Christophe Bron Stanley Juin Ryan R. Hemme Elizabeth Hunsperger Emily S. Jentes Roc Magloire Kay M. Tomashek Anne Marie Desormeaux Jorge L. Mu?oz-Jordán Lesly Etienne Manuela Beltran Tyler M. Sharp Daphne Moffett Jordan Tappero Harold S. Margolis Mark A. Katz 《PLoS neglected tropical diseases》2014,8(10)
In October 2012, the Haitian Ministry of Health and the US CDC were notified of 25 recent dengue cases, confirmed by rapid diagnostic tests (RDTs), among non-governmental organization (NGO) workers. We conducted a serosurvey among NGO workers in Léogane and Port-au-Prince to determine the extent of and risk factors for dengue virus infection. Of the total 776 staff from targeted NGOs in Léogane and Port-au-Prince, 173 (22%; 52 expatriates and 121 Haitians) participated. Anti-dengue virus (DENV) IgM antibody was detected in 8 (15%) expatriates and 9 (7%) Haitians, and DENV non-structural protein 1 in one expatriate. Anti-DENV IgG antibody was detected in 162 (94%) participants (79% of expatriates; 100% of Haitians), and confirmed by microneutralization testing as DENV-specific in 17/34 (50%) expatriates and 42/42 (100%) Haitians. Of 254 pupae collected from 68 containers, 65% were Aedes aegypti; 27% were Ae. albopictus. Few NGO workers reported undertaking mosquito-avoidance action. Our findings underscore the risk of dengue in expatriate workers in Haiti and Haitians themselves. 相似文献
43.
Post-genomics of lactic acid bacteria and other food-grade bacteria to discover gut functionality 总被引:4,自引:0,他引:4
Recent years have seen an explosion in the number of complete or almost complete genomic sequences of lactic acid bacteria and other food-grade bacteria that are used in functional foods to increase the health of the consumer. These have been instrumental in the development of functional, comparative and other post-genomics approaches that provide the possibility to detect, unravel and understand their functionality in the human intestinal tract. In conjunction with other high-throughput approaches, these advances can be exploited in the functional food innovation cycle for developing new or designed probiotic and other bacterial products that impact gut health. 相似文献
44.
In mitochondria, chloroplasts, and Gram-negative eubacteria, Oxa1p(-like) proteins are critical for the biogenesis of membrane proteins. Here we show that the Gram-positive eubacterium Bacillus subtilis contains two functional Oxa1p orthologues, denoted SpoIIIJ and YqjG. The presence of either SpoIIIJ or YqjG is required for cell viability. Whereas SpoIIIJ is required for sporulation, YqjG is dispensable for this developmental process. The stability of two membrane proteins was found to be mildly affected upon SpoIIIJ limitation in the absence of YqjG. Surprisingly, the topology and stability of other membrane proteins remained unaffected under these conditions. In contrast, SpoIIIJ- and YqjG-limiting conditions resulted in a strong post-translocational defect in the stability of secretory proteins. Together, these data indicate that SpoIIIJ and YqjG of B. subtilis are involved in both membrane protein biogenesis and protein secretion. However, the reduced stability of secretory proteins seems to be the most prominent phenotype of SpoIIIJ/YqjG-depleted B. subtilis cells. In conclusion, our observations show that SpoIIIJ and YqjG have different, but overlapping functions in B. subtilis. Most importantly, it seems that different members of the Oxa1p protein family have acquired at least partly distinct, species-specific, functions that are essential for life. 相似文献
45.
46.
47.
Tjalsma H Stover AG Driks A Venema G Bron S van Dijl JM 《The Journal of biological chemistry》2000,275(33):25102-25108
Type I signal peptidases (SPases) are required for the removal of signal peptides from translocated proteins and, subsequently, release of the mature protein from the trans side of the membrane. Interestingly, prokaryotic (P-type) and endoplasmic reticular (ER-type) SPases are functionally equivalent, but structurally quite different, forming two distinct SPase families that share only few conserved residues. P-type SPases were, so far, exclusively identified in eubacteria and organelles, whereas ER-type SPases were found in the three kingdoms of life. Strikingly, the presence of ER-type SPases appears to be limited to sporulating Gram-positive eubacteria. The present studies were aimed at the identification of potential active site residues of the ER-type SPase SipW of Bacillus subtilis, which is required for processing of the spore-associated protein TasA. Conserved serine, histidine, and aspartic acid residues are critical for SipW activity, suggesting that the ER-type SPases employ a Ser-His-Asp catalytic triad or, alternatively, a Ser-His catalytic dyad. In contrast, the P-type SPases employ a Ser-Lys catalytic dyad (Paetzel, M., Dalbey, R. E., and Strynadka, N. C. J. (1998) Nature 396, 186-190). Notably, catalytic activity of SipW was not only essential for pre-TasA processing, but also for the incorporation of mature TasA into spores. 相似文献
48.
49.
Boland JL Corbeij HA Van Der Giessen W Seabra-Gomes R Suryapranata H Wijns W Hanet C Suttorp MJ Buller C Bonnier JJ Colombo A Van Birgelen C Pieper M Mangioni JA Londero H Carere RG Hamm CW Bonan R Bartorelli A Kyriakides ZS Chauhan A Rothman M Grinfeld L Oosterwijk C Serruys PW Cumberland DC 《International journal of cardiovascular interventions》2000,3(4):215-225
AIMS: The BiodivYsio trade mark stent (Biocompatibles Ltd, Farnham, UK) is coated with a phosphorylcholine (PC)-containing copolymer to confer biocompatibility. The SOPHOS (Study Of PHosphorylcholine coating On Stents) study was designed to assess the safety and efficacy of this novel coronary stent and by indirect comparison to indicate equivalence with other formal stent studies. METHODS AND RESULTS: Patients with angina and a single short (#x2A7F;12 mm) de novo lesion in a native coronary artery of >/=2.75 mm diameter were included. A total of 425 patients were allocated in 24 centers. Clinical data were collected at one-, six- and nine-month follow-up. Angiography was performed before and after the stent implantation. In addition, in the first 200 patients (SOPHOS A) angiography was routinely performed at six months. The following 225 patients (SOPHOS B) were merely followed up clinically. The primary end-point of the study, the six-month MACE-rate (MACE = Major Adverse Cardiac Events) was 13.4% (two cardiac death; five Q-wave/nine non-Q-wave myocardial infarctions (MI); nine CABG and 32 target lesion revascularization (TLR), which is similar to the calculated 15% MACE-rate in comparable reference studies. Secondary end-points included among others restenosis at six months in the SOPHOS A population. The target vessel diameter was 2.98 +/- 0.48 mm. Minimal lumen diameter pre/post procedure and at follow-up was 1.00 +/- 0.32, 2.69 +/- 0.37, 1.91 +/- 0.71 mm, respectively. The binary restenosis rate (>/=50% diameter stenosis at follow-up) was 17.7%. CONCLUSION: The coronary BiodivYsio stent is safe and effective as a primary device for the treatment of native coronary artery lesions in patients with stable or unstable angina pectoris. Clinical and angiographic results are in the statistical range of equivalence with comparable studies with other current stents. 相似文献
50.
Yvet E Noordman Patrick AM Jansen Wiljan JAJ Hendriks 《Journal of molecular signaling》2006,1(1):1-11