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581.
Gareth K. Phoenix Bridget A. Emmett Andrea J. Britton Simon J. M. Caporn Nancy B. Dise Rachel Helliwell Laurence Jones Jonathan R. Leake Ian D. Leith Lucy J. Sheppard Alwyn Sowerby Michael G. Pilkington Edwin C. Rowe Mike R. Ashmore Sally A. Power 《Global Change Biology》2012,18(4):1197-1215
Atmospheric nitrogen (N) deposition is a global and increasing threat to biodiversity and ecosystem function. Much of our current understanding of N deposition impacts comes from field manipulation studies, although interpretation may need caution where simulations of N deposition (in terms of dose, application rate and N form) have limited realism. Here, we review responses to simulated N deposition from the UKREATE network, a group of nine experimental sites across the UK in a diversity of heathland, grassland, bog and dune ecosystems which include studies with a high level of realism and where many are also the longest running globally on their ecosystem type. Clear responses were seen across the sites with the greatest sensitivity shown in cover and species richness of bryophytes and lichens. Productivity was also increased at sites where N was the limiting nutrient, while flowering also showed high sensitivity, with increases and declines seen in dominant shrub and forb species, respectively. Critically, these parameters were responsive to some of the lowest additional loadings of N (7.7–10 kg ha?1 yr?1) showing potential for impacts by deposition rates seen in even remote and ‘unpolluted’ regions of Europe. Other parameters were less sensitive, but nevertheless showed response to higher doses. These included increases in soil %N and ‘plant available’ KCl extractable N, N cycling rates and acid–base status. Furthermore, an analysis of accumulated dose that quantified response against the total N input over time suggested that N impacts can ‘build up’ within an ecosystem such that even relatively low N deposition rates can result in ecological responses if continued for long enough. Given the responses have important implications for ecosystem structure, function, and recovery from N loading, the clear evidence for impacts at relatively low N deposition rates across a wide range of habitats is of considerable concern. 相似文献
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Stephen Y K Seah K Linda Britton David W Rice Yasuhisa Asano Paul C Engel 《European journal of biochemistry》2003,270(23):4628-4634
Through comparison with the high-resolution structure of Clostridium symbiosum glutamate dehydrogenase, the different substrate specificities of the homologous enzymes phenylalanine dehydrogenase and leucine dehydrogenase were attributed to two residues, glycine 124 and leucine 307, in Bacillus sphaericus phenylalanine dehydrogenase, which are replaced with alanine and valine in leucine dehydrogenases. As predicted, making these substitutions in phenylalanine dehydrogenase decreased the specific activity towards aromatic substrates and enhanced the activity towards some aliphatic amino acids in standard assays with fixed concentrations of both substrates. This study did not, however, distinguish effects on affinity from those on maximum catalytic rate. A fuller kinetic characterization of the single- and double-mutant enzymes now reveals that the extent of the shift in specificity was underestimated in the earlier study. The maximum catalytic rates for aromatic substrates are reduced for all the mutants, but, in addition, the apparent Km values are higher for the single-mutant G124A and double-mutant G124A/L307V compared with the wild-type enzyme. Conversely, specificity constants (kcat/Km) for the nonpolar aliphatic amino acids and the corresponding 2-oxoacids for the mutants are all markedly higher than for the wild type, with up to a 40-fold increase for l-norvaline and a 100-fold increase for its 2-oxoacid in the double mutant. In some cases a favourable change in Km was found to outweigh a smaller negative change in kcat. These results emphasize the risk of misjudging the outcome of protein engineering experiments through too superficial an analysis. Overall, however, the success of the predictions from molecular modelling indicates the usefulness of this strategy for engineering new specificities, even in advance of more detailed 3D structural information. 相似文献
586.
Is increased chromosomal diversity in house mice from Lombardy (Italy) congruent with genic divergence?
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Claudine Montgelard Josette Catalan Janice Britton‐Davidian 《Biological journal of the Linnean Society. Linnean Society of London》2016,118(2):245-261
Recent studies in metacentric (MC) populations of the house mouse, Mus musculus domesticus, singled out underdominance more so than recombination suppression as the foremost barrier to gene flow. Here, MC populations from Lombardy (Italy) were sampled to identify the nature and strength of the barriers to gene flow. The chromosomal analysis recovered the three major MC populations (abbreviated to IBIN, IGAL, both with 2n = 24 and ICRE, 2n = 22), but revealed the existence of a new one (IONE, 2n = 24) which likely derived from IGAL through a single WART (Whole‐Arm Reciprocal Translocation). This, once again, highlights the paramount role of WARTs in the chromosomal diversification of this subspecies. Contacts between MC and standard populations coincided with rivers confirming these hybrid zones as tension zones. Divergence between populations was estimated using available allozyme data. Although the overall low genetic structure globally agreed with the chromosome structure, a large variation in divergence levels was retrieved that only partially matched the underdominance degree. This disparity from expectations highlighted the additional contribution of physical barriers and geographic isolation to the differential rate of evolution of the MC populations of the house mouse. 相似文献
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Hatchery‐reared fish are commonly stocked into freshwaters to enhance recreational angling. As these fishes are often of high trophic position and attain relatively large sizes, they potentially interact with functionally similar resident fishes and modify food‐web structure. Hatchery‐reared barbel Barbus barbus are frequently stocked to enhance riverine cyprinid fish communities in Europe; these fish can survive for over 20 years and exceed 8 kg. Here, their trophic consequences for resident fish communities were tested using cohabitation studies, mainly involving chub Squalius cephalus, a similarly large‐bodied, omnivorous and long‐lived species. These studies were completed over three spatial scales: pond mesocosms, two streams and three lowland rivers, and used stable isotope analysis. Experiments in mesocosms over 100 days revealed rapid formation of dietary specializations and discrete trophic niches in juvenile B. barbus and S. cephalus. This niche partitioning between the species was also apparent in the streams over 2 years. In the lowland rivers, where fish were mature individuals within established populations, this pattern was also generally apparent in fishes of much larger body sizes. Thus, the stocking of these hatchery‐reared fish only incurred minor consequences for the trophic ecology of resident fish, with strong patterns of trophic niche partitioning and diet specialization. Application of these results to decision‐making frameworks should enable managers to make objective decisions on whether cyprinid fish should be stocked into lowland rivers according to ecological risk. 相似文献
588.
Sebastian A. Stifter Nayan Bhattacharyya Roman Pillay Manuela Flórido James A. Triccas Warwick J. Britton Carl G. Feng 《PLoS pathogens》2016,12(1)
Host control of influenza A virus (IAV) is associated with exuberant pulmonary inflammation characterized by the influx of myeloid cells and production of proinflammatory cytokines including interferons (IFNs). It is unclear, however, how the immune system clears the virus without causing lethal immunopathology. Here, we demonstrate that in addition to its known anti-viral activity, STAT1 signaling coordinates host inflammation during IAV infection in mice. This regulatory mechanism is dependent on both type I IFN and IFN-γ receptor signaling and, importantly, requires the functional interplay between the two pathways. The protective function of type I IFNs is associated with not only the recruitment of classical inflammatory Ly6Chi monocytes into IAV-infected lungs, but also the prevention of excessive monocyte activation by IFN-γ. Unexpectedly, type I IFNs preferentially regulate IFN-γ signaling in Ly6Clo rather than inflammatory Ly6Chi mononuclear cell populations. In the absence of type I IFN signaling, Ly6Clo monocytes/macrophages, become phenotypically and functionally more proinflammatory than Ly6Chi cells, revealing an unanticipated function of the Ly6Clo mononuclear cell subset in tissue inflammation. In addition, we show that type I IFNs employ distinct mechanisms to regulate monocyte and neutrophil trafficking. Type I IFN signaling is necessary, but not sufficient, for preventing neutrophil recruitment into the lungs of IAV-infected mice. Instead, the cooperation of type I IFNs and lymphocyte-produced IFN-γ is required to regulate the tissue neutrophilic response to IAV. Our study demonstrates that IFN interplay links innate and adaptive anti-viral immunity to orchestrate tissue inflammation and reveals an additional level of complexity for IFN-dependent regulatory mechanisms that function to prevent excessive immunopathology while preserving anti-microbial functions. 相似文献
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Pinder Adrian C. Britton J. Robert Harrison Andrew J. Nautiyal Prakash Bower Shannon D. Cooke Steven J. Lockett Steve Everard Mark Katwate Unmesh Ranjeet K. Walton Sam Danylchuk Andy J. Dahanukar Neelesh Raghavan Rajeev 《Reviews in Fish Biology and Fisheries》2019,29(2):417-452
Reviews in Fish Biology and Fisheries - The mahseer fishes (Tor spp.) represent an iconic genus of large-bodied species of the Cyprinidae family. Across the 16 recognised species in the genus,... 相似文献