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51.
Charles Ebikeme Jane Hubert Marc Biran Gilles Gouspillou Pauline Morand Nicolas Plazolles Fabien Guegan Philippe Diolez Jean-Michel Franconi Jean-Charles Portais Frédéric Bringaud 《The Journal of biological chemistry》2010,285(42):32312-32324
Trypanosoma brucei is a parasitic protist that undergoes a complex life cycle during transmission from its mammalian host (bloodstream forms) to the midgut of its insect vector (procyclic form). In both parasitic forms, most glycolytic steps take place within specialized peroxisomes, called glycosomes. Here, we studied metabolic adaptations in procyclic trypanosome mutants affected in their maintenance of the glycosomal redox balance. T. brucei can theoretically use three strategies to maintain the glycosomal NAD+/NADH balance as follows: (i) the glycosomal succinic fermentation branch; (ii) the glycerol 3-phosphate (Gly-3-P)/dihydroxyacetone phosphate (DHAP) shuttle that transfers reducing equivalents to the mitochondrion; and (iii) the glycosomal glycerol production pathway. We showed a hierarchy in the use of these glycosomal NADH-consuming pathways by determining metabolic perturbations and adaptations in single and double mutant cell lines using a combination of NMR, ion chromatography-MS/MS, and HPLC approaches. Although functional, the Gly-3-P/DHAP shuttle is primarily used when the preferred succinate fermentation pathway is abolished in the Δpepck knock-out mutant cell line. In the absence of these two pathways (Δpepck/RNAiFAD-GPDH.i mutant), glycerol production is used but with a 16-fold reduced glycolytic flux. In addition, the Δpepck mutant cell line shows a 3.3-fold reduced glycolytic flux compensated by an increase of proline metabolism. The inability of the Δpepck mutant to maintain a high glycolytic flux demonstrates that the Gly-3-P/DHAP shuttle is not adapted to the procyclic trypanosome context. In contrast, this shuttle was shown earlier to be the only way used by the bloodstream forms of T. brucei to sustain their high glycolytic flux. 相似文献
52.
Bringaud F Ghedin E El-Sayed NM Papadopoulou B 《Microbes and infection / Institut Pasteur》2008,10(6):575-581
Transposable elements constitute 2-5% of the genome content in trypanosomatid parasites. Some of them are involved in critical cellular functions, such as the regulation of gene expression in Leishmania spp. In this review, we highlight the remarkable role extinct transposable elements can play as the source of potential new functions. 相似文献
53.
The generation of energy in African trypanosomes is a subject of undoubted importance. In bloodstream-form organisms, substrate-level phosphorylation of glucose is sufficient to provide the energy needs of the parasite. The situation in procyclic-form trypanosomes is more complex. For many years, it was accepted that glucose metabolism followed a conventional scheme involving glycolysis, the tricarboxylic acid cycle and ATP-producing oxidative phosphorylation linked to the electron-transport chain. However, progress in sequencing the Trypanosoma brucei genome and the development of gene-knockout and RNA interference technology has provided novel insight. Coupling these new technologies with classical approaches, including NMR and mass spectrometry to analyse glycolytic intermediates and end products, has yielded several surprises. In this article, we summarize how these recent data have helped to change the view of metabolism in procyclic-form T. brucei. 相似文献
54.
Kinetoplastida are protozoan organisms that probably diverged early in evolution from other eukaryotes. They are characterized
by a number of unique features with respect to their energy and carbohydrate metabolism. These organisms possess peculiar
peroxisomes, called glycosomes, which play a central role in this metabolism; the organelles harbour enzymes of several catabolic
and anabolic routes, including major parts of the glycolytic and pentosephosphate pathways. The kinetoplastid mitochondrion
is also unusual with regard to both its structural and functional properties. 相似文献
55.
Stefan Allmann Pauline Morand Charles Ebikeme Lara Gales Marc Biran Jane Hubert Ana Brennand Muriel Mazet Jean-Michel Franconi Paul A. M. Michels Jean-Charles Portais Michael Boshart Frédéric Bringaud 《The Journal of biological chemistry》2013,288(25):18494-18505
All living organisms depend on NADPH production to feed essential biosyntheses and for oxidative stress defense. Protozoan parasites such as the sleeping sickness pathogen Trypanosoma brucei adapt to different host environments, carbon sources, and oxidative stresses during their infectious life cycle. The procyclic stage develops in the midgut of the tsetse insect vector, where they rely on proline as carbon source, although they prefer glucose when grown in rich media. Here, we investigate the flexible and carbon source-dependent use of NADPH synthesis pathways in the cytosol of the procyclic stage. The T. brucei genome encodes two cytosolic NADPH-producing pathways, the pentose phosphate pathway (PPP) and the NADP-dependent malic enzyme (MEc). Reverse genetic blocking of those pathways and a specific inhibitor (dehydroepiandrosterone) of glucose-6-phosphate dehydrogenase together established redundancy with respect to H2O2 stress management and parasite growth. Blocking both pathways resulted in ∼10-fold increase of susceptibility to H2O2 stress and cell death. Unexpectedly, the same pathway redundancy was observed in glucose-rich and glucose-depleted conditions, suggesting that gluconeogenesis can feed the PPP to provide NADPH. This was confirmed by (i) a lethal phenotype of RNAi-mediated depletion of glucose-6-phosphate isomerase (PGI) in the glucose-depleted Δmec/Δmec null background, (ii) an ∼10-fold increase of susceptibility to H2O2 stress observed for the Δmec/Δmec/RNAiPGI double mutant when compared with the single mutants, and (iii) the 13C enrichment of glycolytic and PPP intermediates from cells incubated with [U-13C]proline, in the absence of glucose. Gluconeogenesis-supported NADPH supply may also be important for nucleotide and glycoconjugate syntheses in the insect host. 相似文献
56.
Maria‐Rosa Garcia‐Silva Lauriane Sollelis Cameron Ross MacPherson Slavica Stanojcic Nada Kuk Lucien Crobu Frédéric Bringaud Patrick Bastien Michel Pagès Artur Scherf Yvon Sterkers 《EMBO reports》2017,18(11):1968-1977
Leishmania affects millions of people worldwide. Its genome undergoes constitutive mosaic aneuploidy, a type of genomic plasticity that may serve as an adaptive strategy to survive distinct host environments. We previously found high rates of asymmetric chromosome allotments during mitosis that lead to the generation of such ploidy. However, the underlying molecular events remain elusive. Centromeres and kinetochores most likely play a key role in this process, yet their identification has failed using classical methods. Our analysis of the unconventional kinetochore complex recently discovered in Trypanosoma brucei (KKTs) leads to the identification of a Leishmania KKT gene candidate (LmKKT1). The GFP‐tagged LmKKT1 displays “kinetochore‐like” dynamics of intranuclear localization throughout the cell cycle. By ChIP‐Seq assay, one major peak per chromosome is revealed, covering a region of 4 ±2 kb. We find two largely conserved motifs mapping to 14 of 36 chromosomes while a higher density of retroposons are observed in 27 of 36 centromeres. The identification of centromeres and of a kinetochore component of Leishmania chromosomes opens avenues to explore their role in mosaic aneuploidy. 相似文献
57.
Ebikeme CE Peacock L Coustou V Riviere L Bringaud F Gibson WC Barrett MP 《Parasitology》2008,135(5):585-594
SUMMARYThe lectin-inhibitory sugars D-glucosamine (GlcN) and N-acetyl D-glucosamine (GlcNAc) are known to enhance susceptibility of the tsetse fly vector to infection with Trypanosoma brucei. GlcNAc also stimulates trypanosome growth in vitro in the absence of any factor derived from the fly. Here, we show that GlcNAc cannot be used as a direct energy source, nor is it internalized by trypanosomes. It does, however, inhibit glucose uptake by binding to the hexose transporter. Deprivation of D-glucose leads to a switch from a metabolism based predominantly on substrate level phosphorylation of D-glucose to a more efficient one based mainly on oxidative phosphorylation using L-proline. Procyclic form trypanosomes grow faster and to higher density in D-glucose-depleted medium than in D-glucose-rich medium. The ability of trypanosomes to use L-proline as an energy source can be regulated depending upon the availability of D-glucose and here we show that this regulation is a graded response to D-glucose availability and determined by the overall metabolic state of the cell. It appears, therefore, that the growth stimulatory effect of GlcNAc in vitro relates to the switch from D-glucose to L-proline metabolism. In tsetse flies, however, it seems probable that the effect of GlcNAc is independent of this switch as pre-adaptation to growth in proline had no effect on tsetse infection rate. 相似文献
58.
C Vedrenne F Bringaud M P Barrett E Tetaud T Baltz 《European journal of biochemistry》2000,267(15):4850-4860
We have previously characterized, in Trypanosoma brucei, a multigene family encoding two developmentally regulated glucose transporters that are 80% identical at the amino-acid level. We report here the characterization of the homologous glucose transporters (TcoHT1 and TcoHT2) in Trypanosoma congolense, an African trypanosome responsible for disease in domestic animals. Both TcoHT isoforms, which are 92.4% identical, are encoded by a single cluster of genes containing two copies of TcoHT1 and three copies of TcoHT2 arranged alternately. Northern blot analysis revealed that TcoHT2 is expressed in all of the adaptive forms, while mRNA encoding TcoHT1 is only present in the metacyclic and bloodstream forms of T. congolense. When transfected with the TcoHT2 gene, Chinese Hamster Ovary cells express a hexose transporter with properties similar to those of the T. congolense procyclic forms (Km D-glucose = 41 microM versus 64 microM). In contrast to TcoHT2, TcoHT1 expressed in the Chinese hamster ovary cells appeared to be a relatively low affinity glucose transporter (Ki D-glucose = 0.8 mM). To determine the region(s) involved in the different apparent affinities for glucose, a chimera analysis was undertaken on the TcoHT isoforms. This study shows that amino-acid residues important for D-glucose recognition are located in the central region (between transmembrane domains 3 and 7) and in the C-terminal intracellular domain of TcoHT2. Site directed mutagenesis identified Ser193 located within transmembrane helix 4 as a key residue in relaxing the apparent affinity of TcoHT1 for glucose. 相似文献
59.
Adrian R Walmsley Michael P Barrett Fredéric Bringaud Gwyn W Gould 《Trends in biochemical sciences》1998,23(12):71-481
Sugar transport across the plasma membrane is one of the most important transport processes. The cloning and expression of cDNAs from a superfamily of related sugar transporters that all adopt a 12-membrane-spanning-domain structure has opened new avenues of investigation, including pre-steady-state kinetic analysis. Structure–function analyses of mammalian and bacterial sugar transporters, and comparisons of these transporters with those of parasitic trypanosomatids, indicate that different environmental pressures have tailored the evolution of the various members of the sugar-transporter superfamily. Subtle distinctions in the function of these proteins can be related to particular amino acid residue substitutions. 相似文献
60.
African trypanosome glucose transporter genes: organization and evolution of a multigene family 总被引:1,自引:0,他引:1
Trypanosoma brucei brucei (EATRO-164) contains a tandem array of six genes
encoding a glucose transporter, THT1 (trypanosome hexose transporter),
followed by five genes encoding a second isoform, THT2. Two distinct
clusters containing THT1 and THT2 genes have been identified in the
EATRO-164 clone and in most other African trypanosome clones analyzed.
Analysis of progeny from crosses between clones of T. b. brucei displaying
polymorphism in THT1 copy number per cluster suggests that the two clusters
of THT genes are present on homologous chromosomes. In addition, analysis
of 30 African trypanosome clones revealed a high degree of polymorphism in
THT1 copy number per cluster. Sequence comparison of five THT1 and two and
one-half THT2 unit repeats, present within a 20-kb region, provided
information about the genesis and evolution of the THT multigene family.
The most divergent regions between THT1 and THT2 unit repeats probably
arose from insertion of DNA fragments into an ancestral THT region. Genes
of each of the different families are almost identical, and there are large
regions of identity shared between THT1 and THT2 members. A mosaic copy
containing most of a THT1 gene with the 3' extremity of a THT2 gene is
found within the cluster. These results suggest that THT1 and THT2 arose by
modification (insertion, mutation, or conversion) of duplicated ancestral
genes. Functional constraints and homologous recombination may be evoked to
explain the maintenance of the conserved sequences of THT1 and THT2.
相似文献